LOCUS       NZ_AP026416             3076 bp    DNA     circular CON 16-APR-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM644
            plasmid pKAM644_9, complete sequence.
ACCESSION   NZ_AP026416
VERSION     NZ_AP026416.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446297
            Assembly: GCF_024347795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 189x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024347795.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 09:13:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,149
            CDSs (total)                      :: 6,034
            Genes (coding)                    :: 5,821
            CDSs (with protein)               :: 5,821
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 99 of 213
            Pseudo Genes (incomplete)         :: 134 of 213
            Pseudo Genes (internal stop)      :: 36 of 213
            Pseudo Genes (multiple problems)  :: 46 of 213
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026416.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM644"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM644_9"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(576..962)
                     /locus_tag="P4I75_RS30665"
                     /old_locus_tag="KAM644c_59710"
     CDS             complement(576..962)
                     /locus_tag="P4I75_RS30665"
                     /old_locus_tag="KAM644c_59710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            1129..2064
                     /locus_tag="P4I75_RS30670"
                     /old_locus_tag="KAM644c_59720"
     CDS             1129..2064
                     /locus_tag="P4I75_RS30670"
                     /old_locus_tag="KAM644c_59720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            2067..2597
                     /locus_tag="P4I75_RS30675"
                     /old_locus_tag="KAM644c_59730"
     CDS             2067..2597
                     /locus_tag="P4I75_RS30675"
                     /old_locus_tag="KAM644c_59730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     Typing          1130..2061
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagaatacca tattagggca aagaaagcct aatgcccccg tggtgggtac accagaaaac
       61 ggggagcgaa gcgaccagca tcaggccgtt cgtatgctgc ttcgattttg tgggtgttgc
      121 gccagacctt ggcaatcacg cttcaccgca accgctgcct tcactctcac tttatcaggt
      181 acgttattgg ttaacgtgag accgggtaga gccagcttct ttcccgagct ggtcaggttc
      241 ccaccgcttg cctgtttatc ctgccggagt ccggggcgta gacctgagag tcaaggtgca
      301 gatatgggtt taatgtcgtt gaatggtatt aagctggtag tgacattcct agccagccct
      361 gctaggattt gattgtgacg tctgatcctg gcaggtatct gcaagaatta gagtactcaa
      421 gccccgtgat tctgtcacgg ggcttttgta catctggagc aagaaaattc atgctcctat
      481 ccgaaaatcc ctaccgctcg ccgcagccga acgaccgagc gacgcgagtc agtgagcgag
      541 gaagcggcat ttctccggcc ttgcagaatg cacgttcacg ccgatgtttc cagatcgtag
      601 cgtacccgtt tcagcgcagt cagtaatttt tcgtggtcaa aattcaactc acccgttttt
      661 gtatcaacca gtcccgcgtc aatcatcaga ccagccgcca gaaacagtct gtgattgcgt
      721 tccctgcggt ttttctgccc tgaaatccgc gcctgaaccg cctttttctg tgcggcatcc
      781 cgttcctgca acgcgttttt ttcagcggtt gccagaacgt ggagcagtcg tctttcagtc
      841 tcactgagcg aagcttcatc atccagcaga tcgataatct gccgttgccg tgccgttttt
      901 tctcccagct ttctgatgaa ggctatgcgc tttttaagcc attcgacgtt actgatccgc
      961 atcgtttaac ttccccatcc cgatatttcc gacggggaaa gtatataact tgcggtcaga
     1021 agtcgcatga cttatcttta ctcataattc tttaaattag agcaaagcgc acttatatgt
     1081 ttctccctgc gggcagaaac agtgcgccct gccgggggct gacacaacat ggcaacgtat
     1141 catctcagcg tgaaatttgg cggcaagggt aaagcccttg ctcatgccag ttacattacg
     1201 cgggaagata agttcagcca gcgccaggat ctcgagcaca ccgaacacgg caatatgccg
     1261 gaatgggcga gagatgaacc cgcccatttc tggcaggctg cggatgcgtt cgaacgtgcc
     1321 aacggttcaa cgtacaggga aattgagatc gctctacccc gcgagctgaa tgaagcgcag
     1381 cggctggcgc ttgtccggga ttttgtgaag caggaaacgg gcgacaaaca tgcatggacg
     1441 tttgcgatcc acaatccgaa agccagcatt gacggcggcg aacagccgca cgcccatatc
     1501 atgatgagcc agcgcgtcaa tgacggtatt gagcgcagtc ccgaacagta tttcaggcgc
     1561 tataacgcca aatttccggc gcgtggcggc gcgcgcaagg actcagggca taatttaacc
     1621 cctacagaac aaaaacaggc actgaaagcc ctgcgggagc gctgggagcg tacgcacaac
     1681 gcgcatatga aacgatatgg ctttgagcag ggttttattg atagccggtc actgaaaaca
     1741 cagggtatcg accggaaacc ggaacagcat ctcggttttg aagcagccgg acgactggac
     1801 gacagccaga ggcaggatat taaagacagc cggacgtttt cagtcgtgga tgtgcctgaa
     1861 caggcgaaac atatcgacca cagcgaccgt agctggatga atgccagcgg tatcgaggta
     1921 aaccggaagg aactggtcag atctctgacg catgatatct cgcagcagta cgggcgggag
     1981 atggctgcac gggtcacaaa acagggaaat gttgaaatag agcgcgtgaa atccctgtca
     2041 cgcggatttt ccataagcag gtaactatgg cgagcaccga agaaaaactt ctcacactcc
     2101 tgctgtcggc ggagcatctg aaagaggccg caaaagagca gcagaaagcc gtggcggatt
     2161 cgctggcggt ggcaagggaa accctgaaag gctaccggga aatatcggac agtgtggcac
     2221 agcaggtaag ggatgaagtc cggcaggaag tgcagaaaat ggatgttgcg gggctgattg
     2281 gcgagcgtat cagcgctgat tttcagcgtc tggaaaaatc cgtcgggaac atgaaaatca
     2341 atgcggacgc gctggacaag gaaatttcca ttatccgcag taacatcatc gcggagtaca
     2401 acacgctgcg gggttacagc tggaagtggt ttgctggggt tgcggttgcc tttgtcgtac
     2461 tgatatttgc cacaagctgg tttgtgaaaa cggaactaca ggacaattac aacgcgtctg
     2521 ctgccgtcta tcagaaagtg gcagaccttg aaaagctgct gacaacggaa cagaaaccgg
     2581 aaaagaaaaa gaactgacgc gctgcgcttg tccaacgcct cacgttcgca caataaattg
     2641 cgcacccgtt caccgttttc agccgagttt ggggcgtctt ttgagacttc cggcctgaat
     2701 tcaggccgga agttctcgcc agtcagtcag gtgcatggtt atgcatgggg ctgaaaatca
     2761 cggtatgcga ttctgagcgg ttttcggtga gaagccgggg gtttttatgg ctcaggacgt
     2821 aaaaccgccc tgagcgcgtt tcagcggtgc gcgtaacgga tgttatggta aatccggtgg
     2881 ctttgacgtg cctgccgttg tgtaccgtcg tgagtgaaaa taattatatt gttcgaagaa
     2941 cagcgctttt tggccggaga aaagcagttc tgcaaacgat ggtatatcga tatgagttct
     3001 tttggcatta atccaggcaa gacgccgggc aataagcgta atattccgga tgttacttat
     3061 ttccatttct gtcaaa
//
