LOCUS       NZ_AP026415             4730 bp    DNA     circular CON 16-APR-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM644
            plasmid pKAM644_8, complete sequence.
ACCESSION   NZ_AP026415
VERSION     NZ_AP026415.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446297
            Assembly: GCF_024347795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 189x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024347795.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 09:13:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,149
            CDSs (total)                      :: 6,034
            Genes (coding)                    :: 5,821
            CDSs (with protein)               :: 5,821
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 99 of 213
            Pseudo Genes (incomplete)         :: 134 of 213
            Pseudo Genes (internal stop)      :: 36 of 213
            Pseudo Genes (multiple problems)  :: 46 of 213
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026415.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM644"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM644_8"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            join(4533..4730,1..234)
                     /locus_tag="P4I75_RS30635"
     CDS             join(4533..4730,1..234)
                     /locus_tag="P4I75_RS30635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086073960.1"
                     /translation="MDWYDVKWFFGDVCDSVSEVAGNIYDTAADGVAAVTDFIEENPKT
                     SIAIGALVGIATAGIAAPAIAATIGATGALGTTAGGTVIANLSGAALSSSSLAALGGGS
                     IAAGGAGMAGGTCVVAAGGGVVAGSVSAGGVAATKAIKS"
                     /name="hypothetical protein"
     gene            complement(322..666)
                     /locus_tag="P4I75_RS30640"
                     /old_locus_tag="KAM644c_59660"
     CDS             complement(322..666)
                     /locus_tag="P4I75_RS30640"
                     /old_locus_tag="KAM644c_59660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032431017.1"
                     /translation="MANSFNVTIAEAALWSLQDAESFKSNYTDSVQAALWADDLLASSI
                     KAISQDPDRYRYNAMLADKGISIRERLDPDNQYRCLYDFDEQNGVIEILLFVSTKQDLE
                     KMLYRYLVVK"
                     /name="hypothetical protein"
     gene            complement(681..950)
                     /locus_tag="P4I75_RS30645"
                     /old_locus_tag="KAM644c_59670"
     CDS             complement(681..950)
                     /locus_tag="P4I75_RS30645"
                     /old_locus_tag="KAM644c_59670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024167138.1"
                     /translation="MSITMSQKSAREGLGNPELFQGGVYVTKNGLAELFVQTAEEREAE
                     LRERERERQSNALLKLVMAAKREIKNGRGMSPEDAMRRLREART"
                     /name="hypothetical protein"
     gene            complement(1990..2412)
                     /locus_tag="P4I75_RS30650"
                     /old_locus_tag="KAM644c_59680"
     CDS             complement(1990..2412)
                     /locus_tag="P4I75_RS30650"
                     /old_locus_tag="KAM644c_59680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2412..2603)
                     /locus_tag="P4I75_RS30655"
                     /old_locus_tag="KAM644c_59690"
     CDS             complement(2412..2603)
                     /locus_tag="P4I75_RS30655"
                     /old_locus_tag="KAM644c_59690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3023..3136
                     /locus_tag="P4I75_RS30805"
     CDS             3023..3136
                     /locus_tag="P4I75_RS30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071785923.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            3544..4215
                     /locus_tag="P4I75_RS30660"
     CDS             3544..4215
                     /locus_tag="P4I75_RS30660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223364945.1"
                     /translation="MLIVILSLYYTITEPFKSELIKNLSYSLIATFIFDTGLNFSKENI
                     TKSIVSRKWHNSLYFAFERLRAIHEIFTAQNKLEITKELASDMASSILMENGAVISKKH
                     IKTMWNPSSGEPLTYQDIVIKEGDNLYFASMMFIRDDYEFLHSIYFDENIFLSFPQLLK
                     PAQRCYRSLAMIQHNINSKQKFNVTKKSLERDLINYLELRSTLLMKMEDVLGHYEQRAP
                     Q"
                     /name="hypothetical protein"
     Typing          2725..3136
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.22"
                     /coverage="99.0"
                     /evalue="2.13e-170"
ORIGIN
        1 gcaacaattg gagcaaccgg agcgctagga actacagctg gaggtaccgt aatagcaaac
       61 ttaagtggag cagcattatc tagctcgtct ttagcagcgt tgggtggagg ttccatcgca
      121 gctggaggag ccggtatggc tggcggaaca tgtgtagtag ccgcgggagg aggagtggtc
      181 gctggaagcg tttctgctgg aggagtggca gcaacaaaag cgataaaaag ctaacaagct
      241 tccatttacc ataacgtcga catcttaaag tttctaccat ataaaaagcc cactataaaa
      301 gcgggctttt gcatttaaca tctatttaac aacaagatat ctgtaaagca ttttctcaag
      361 atcttgctta gtgctaacaa aaagcaggat ctcaatgaca ccattttgtt catcaaaatc
      421 atataaacaa cgatactggt tatcggggtc caaacgctca cgaatcgata ttcctttatc
      481 agccaacata gcattgtagc gataacgatc aggatcttga gaaatggcct ttatcgatga
      541 agccaaaaga tcatcagccc ataacgcagc ttgaacacta tccgtgtagt ttgatttaaa
      601 ggattctgca tcctgcagag accatagtgc cgcttccgcg atcgttacat tgaatgaatt
      661 tgccaagttg gctccttata ttaagttcgc gcttcacgta acctccgcat agcatcttcg
      721 ggggacattc cacgaccatt ttttatctct ctctttgcag ccatgactag cttcaaaaga
      781 gcatttgatt gccgttctct ttctctctca cgcagctcgg cctctctctc ttctgcggtc
      841 tgcacgaaca gctctgctaa accatttttc gtaacataaa cgcctccttg gaacagttca
      901 gggttgccaa gaccttcacg ggctgacttc tgagacatag ttatagacat acttatacct
      961 ttaattaaaa cagccactta cacgcaagat taagcataag cgactagatc ctgaatgaaa
     1021 aagaggtcaa aaagacctca catcttgctg ccacttaggg tgaatggcag ttatattgta
     1081 tcaaaactca caggggagta caaatttatc ataaatatat acaccaccat tcaaatataa
     1141 gattctggag cttcgaccaa cctcctatta aacgaaaatt acgcgcatca gtaaggcatc
     1201 cccagctcag gaacaatccc cattcttcta ctatcgagat tttttcgtct gttatttaac
     1261 ataaagccat gattgtacgc accgctaaaa cggccagggg gcgtttctga cgggaaaccg
     1321 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     1381 gcatgcatga gtatgcgcgc aagtgcatag cgcggaaatt cacgccattc agccagggta
     1441 agtcctgttt ccggcgttct tctgatccga aaacggtgaa cggggacgca atttattgcg
     1501 tggccgtgag gcgttgccgg cgcagccgga aaaccggttt tcagcacgtt caggaatcgg
     1561 gttcgtcgac ggccggcaat cacgttatgg caagtcgccg gacgactcac cacacgattc
     1621 accggcgatt cgaatcgtga attgaatcaa gagtcaccat gaacaaaaac gcccaaattg
     1681 atgaattgaa tcaagagtcg attcatcata acaacatgat aataaagcgt gattcacata
     1741 tgaatcgtgg gcaattcgta aacgattcgc catagagtcg tccacgactt ggatctgcac
     1801 tttgtaacac atcactgcac attaattttt cattaacaaa ttgttttaaa taaggaatct
     1861 gcaaaatttt aattttgcgt agtgtgtggg tatttttagt ggtgaggagc gttcagagat
     1921 ctggcgattt tttggaaggt tcaggaactc cccggaatgg ccggagagga aaactgaagc
     1981 atgacggggt caggattttt tccagaattt ccaccacggg gaatggggtg gtgaagaagg
     2041 ttcgtgcttg tgctccagaa gctgcatagc ttgacgaagg gaatcaatat gctcatctcg
     2101 agccttaaca gcccctttaa gaccatcgtt ttcagcttct agtaaagcga ttctatgttg
     2161 taaatcatgt aaaccaacgc tgttaacacc tgtagattct gcgtcacttt ttacatcata
     2221 ttttacagta tcgatgacac catcaagaga gccaaacact cgaataagct ctgaagtatc
     2281 aataacttta atagccttcc cgtgctttac aacagaggtg taacttaact tcccactgtt
     2341 aatcatcttg taaattgtgg ggcgactaac cttaacaagc cgtgccgctt cactaataga
     2401 cactctggcc atcagttacc atcctgtaat tcatccagtc tcgaggacag tgtacggaaa
     2461 agatgctccg catcatcgtg gagtttctca caaaggtcgg cctcaacatc aagatcaagc
     2521 tcgttaagct tctccagcag gagcagagac tgattctgga tgaatttagc catgttcagc
     2581 gctgttttct gttgtttcgt cataagaggc ctcagagcgt cagtgctcag tgtagggatg
     2641 ttctcttttc cgcttcctcg ctcactcagt cgctacgctc ctgccgttcg gctgcggcga
     2701 gcggcaggag catgcgtaga aacgctcgag aaaaccgagc aatcgcatgg aggaaagaag
     2761 gaaaggccgc gcagcggccg tttttccata ggctccgccc ccccccctga caagcatcac
     2821 agaaactgac gctcaaatca gtggtggcga aacccgacag gactataaag atatcaggcg
     2881 tttccccctg atggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     2941 attccgctgt tatggccgcg tttatctcat tccacgcctg acactcagtt ccgggtaggc
     3001 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctt
     3061 atccggtaac tatcgtcttg agtccaaccc agaaagacac gacttatcgc cactggcagc
     3121 agccactggt aactgattta gaggagttag tcttgaagtt atgcgccagt taaggctaaa
     3181 ctaaacgaac agttttggtg actgtgctcc tctaactagc cagttaccta ggttcaacga
     3241 gcgccaactc ttagaacctt cgaaaaaacc gccctgcaag gcggtttttt cttattagca
     3301 gggaagagat tacgatgact tgctgaagat ctcaagaaaa tcattcatca ccgatatcga
     3361 ggagtgataa gattggttaa ctttatatta ttcattatat tattcacata cacatgtatt
     3421 ttttgtttca cccccccgag gagacaatat aaatcaatat ctctaattcc tcaaaaaata
     3481 aaagcaccat taaatagata tcgtttatac atatattcat ctggtgcaat acgacaatca
     3541 ctaatgttaa ttgttatatt atctctctat tatacaatta cagaaccatt taaatcagaa
     3601 ttaattaaaa acttatccta ttcattaatc gcaactttta tttttgacac cggcttaaac
     3661 ttcagcaaag aaaatataac aaaaagtata gtatccagga aatggcacaa ttctttatac
     3721 tttgcatttg aaagattaag agcaatacat gaaatattca cagcacaaaa caaactagag
     3781 atcacaaaag aactagccag cgacatggcg tcatccatac taatggaaaa tggagctgtt
     3841 atatctaaaa aacacataaa aacaatgtgg aacccttctt caggagaacc acttacgtat
     3901 caagacatag taataaaaga aggggataac ttatactttg cgtctatgat gttcatcaga
     3961 gatgattatg aattcctaca cagtatttat tttgatgaaa atatatttct ctccttccct
     4021 caactactaa aaccagccca aagatgttac cgatcactag caatgataca gcataatata
     4081 aacagcaaac aaaaatttaa cgtcacaaaa aaatcattag aaagagatct aattaattat
     4141 ttagaactga gatctacatt attaatgaaa atggaagatg ttcttggcca ctatgaacaa
     4201 agagcaccac aataatatca atgtcacttt catctttaaa tttaaaaaag gcaatgagat
     4261 aagtaagatt gaacataaag aaatgattgt gcataaataa aaaacgtttg gcgtcccccc
     4321 ccctgaaagc tatttgtagc aactttcaat ccaatctatc gaaccatcga agaatttacc
     4381 aacataacca gttccactca ccataattat gcatgggcct gcgatcattt tattgccttt
     4441 aaaaaacatt gcttttaata tgtgtattat atcttccgtg caatcaaaaa agattaatta
     4501 cttaataaat catatggtta agagggtagc atatggactg gtacgacgtt aaatggtttt
     4561 tcggagacgt ctgtgactct gtttcagagg tggcaggcaa tatttacgat actgcagctg
     4621 atggggtagc ggctgtaact gatttcattg aagaaaatcc aaaaacatcc attgcgatag
     4681 gagcccttgt aggtattgcg acagcaggca tagcagctcc ggcaatagcg
//
