LOCUS       NZ_AP026401             2290 bp    DNA     circular CON 17-APR-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622
            plasmid pKAM622_9, complete sequence.
ACCESSION   NZ_AP026401
VERSION     NZ_AP026401.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446295
            Assembly: GCF_024347755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024347755.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 10:36:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,129
            CDSs (total)                      :: 6,017
            Genes (coding)                    :: 5,593
            CDSs (with protein)               :: 5,593
            Genes (RNA)                       :: 112
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 424
            CDSs (without protein)            :: 424
            Pseudo Genes (ambiguous residues) :: 0 of 424
            Pseudo Genes (frameshifted)       :: 118 of 424
            Pseudo Genes (incomplete)         :: 273 of 424
            Pseudo Genes (internal stop)      :: 123 of 424
            Pseudo Genes (multiple problems)  :: 78 of 424
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026401.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM622"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM622_9"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            169..612
                     /locus_tag="P4I30_RS30600"
                     /old_locus_tag="KAM622c_60110"
     CDS             169..612
                     /locus_tag="P4I30_RS30600"
                     /old_locus_tag="KAM622c_60110"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_276086063.1"
                     /translation="MKDLLIHYERPVDSFDHNFKIESAKAFSGYITKISKKLWPRKTYH
                     ASAYSFRHAKATELKNSDYDKEKIAQIMGHASVRSQQSYGRKNKKSKGGFNDIEDIKTN
                     VKPRGADRLLRFKIASKNQAAAKIADTSTPSSPPPAPVRRFKM"
                     /name="tyrosine-type recombinase/integrase"
     gene            1159..1443
                     /locus_tag="P4I30_RS30605"
                     /old_locus_tag="KAM622c_60120"
     CDS             1159..1443
                     /locus_tag="P4I30_RS30605"
                     /old_locus_tag="KAM622c_60120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_276086062.1"
                     /translation="MEKYDLTPEQKVAVYEFHAKAYKKNKTLVISETKENKFKSISEGV
                     EYVHLFPKNLKILIKKYGLNTNELLVLTEIMESMLSHGNLYIFTRKFIN"
                     /name="hypothetical protein"
     gene            1478..1798
                     /locus_tag="P4I30_RS30610"
                     /old_locus_tag="KAM622c_60130"
     CDS             1478..1798
                     /locus_tag="P4I30_RS30610"
                     /old_locus_tag="KAM622c_60130"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_276086064.1"
                     /translation="MNKSTMCKTFKTLKQKQCLIEKNGHIYLNSVIFMKGLPHKLFMQF
                     RDHFLNSISYKLDDDEEFEKVFDDNFIKAYEKNLKEIKKKKQQIKEKKISKALDKNIKS
                     IR"
                     /name="hypothetical protein"
     Typing          673..857
                     /name="Col(IRGK)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="1.6600000000000002e-73"
ORIGIN
        1 acactggggt taaaataaat tacgataaac aaaaaaatga aattagatta acaaaaaaat
       61 gaaaaaaact taatcgaaga atgaaaagag gtattggaat tcgtgagttt tcagtaaaaa
      121 tcaataatga taatgaaaga tttttcaaag gaattattga tctaataaat gaaagacctg
      181 ttgattcatt atgaaagacc tgttgattca ttcgatcata attttaaaat tgaaagtgca
      241 aaggcatttt ccggatatat tacaaaaata agcaaaaagc tatggccccg aaaaacttat
      301 catgcttctg catattcttt caggcatgca aaagcaactg agttgaaaaa ttcagattat
      361 gataaagaaa aaatagccca aataatgggc catgcgtcag ttagatctca gcaaagttac
      421 ggtagaaaaa acaaaaaaag caaaggtggg tttaacgaca ttgaagatat aaaaactaat
      481 gttaaacccc gtggtgcaga tagattgcta aggttcaaaa ttgccagcaa aaaccaagca
      541 gcggcaaaaa ttgccgatac ttccaccccc agcagtcctc caccggctcc cgttcgtcgc
      601 tttaaaatgt gaaccgtgag cagttcagga ggtcccctcc tggactgtga agggttggcc
      661 tggcggcgag ccggttttct agcaaaatcc tccatattgc gaagcagggc ccggaaccgg
      721 gtaactggat ggttttcccc tgtgggggat tgatctgtta cttgaaaacc aatgatctta
      781 aaaaccatct caaaagttga aaatttcacc cccttagtgt tcttaaaatt cttagatgtt
      841 cttaggggtt taaaaactac tctccaacca ttgatctaac tgacttttta aaaaaggcag
      901 ttgtcaaaaa cttcaaccgt agttgtcaaa ttcgtcaact ccagttgtca aattcgtcaa
      961 ctgaggttgt caaatccgac aactctagcc agtttccaag tagaaactac agtttcaaaa
     1021 ctgcaacttt ttctactttt tgcaacttaa tctattgact agttttatat aaatgttaaa
     1081 acatatatat agaaataaat aaaaaaagga ggtttctatg gatttaggaa atttgattac
     1141 gaaaaattag atttgttttt ggaaaaatat gatttaacac cagaacaaaa agttgcagtt
     1201 tatgaatttc acgcaaaagc ttataaaaaa aataaaactt tagttatttc tgaaacaaaa
     1261 gaaaataaat ttaaatctat ttccgaaggt gttgaatacg tgcatttatt cccaaaaaat
     1321 ttaaaaattt taattaaaaa atatggttta aatacaaacg aattattggt tttaacggaa
     1381 ataatggagt caatgctttc acacggaaat ttatatattt tcacacggaa atttattaat
     1441 taatttttcg caaaaggcac tttgcgaatt aacaggaatt aataaatcta caatgtgtaa
     1501 aacatttaaa accctcaaac aaaagcagtg tttaattgag aaaaacggac atatttattt
     1561 aaattctgtg atatttatga aagggttacc tcataaattg tttatgcaat ttagagatca
     1621 ttttttaaat tctatctcat ataaattaga tgatgatgaa gaatttgaaa aagtcttcga
     1681 cgataatttt attaaagcat acgaaaaaaa tctcaaagag attaaaaaga aaaagcaaca
     1741 aataaaagaa aagaaaatat caaaagcatt agataaaaat atcaaaagca ttagataatt
     1801 ttgaaaaaga aatctcgaaa gaatggaaag aaaagtttaa agacgaagag gaaaatttcg
     1861 aatttggttt tgaatcggaa atataaaacc gccctcgccg ggcaggccaa tcccttactg
     1921 aaatagaata aattctattc cactaaggga tttttttatt cattgtttct ccacatttgc
     1981 aatattgaca ttaacttcca cccggatata acagtagtat aagttgttgt ttcaacccgt
     2041 ctttttgggt ggaacaacaa ggcattttat gggtaaagcg aagcgaaccc gtaaaactgc
     2101 cacccccaac cgggggtcgt tgttcgattt tgagcgacag cgaaaaaata gaacataagg
     2161 ggggagggtt tgggttttac ggtattttaa atatgagcaa agcgaatttt taaaatgtcc
     2221 ggttctttaa attttgcaat gaggaaaaac tattatgggt aattcaaaaa gaaatataaa
     2281 taaattaaat
//
