LOCUS       NZ_AP026400             3076 bp    DNA     circular CON 17-APR-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622
            plasmid pKAM622_8, complete sequence.
ACCESSION   NZ_AP026400
VERSION     NZ_AP026400.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446295
            Assembly: GCF_024347755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024347755.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 10:36:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,129
            CDSs (total)                      :: 6,017
            Genes (coding)                    :: 5,593
            CDSs (with protein)               :: 5,593
            Genes (RNA)                       :: 112
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 424
            CDSs (without protein)            :: 424
            Pseudo Genes (ambiguous residues) :: 0 of 424
            Pseudo Genes (frameshifted)       :: 118 of 424
            Pseudo Genes (incomplete)         :: 273 of 424
            Pseudo Genes (internal stop)      :: 123 of 424
            Pseudo Genes (multiple problems)  :: 78 of 424
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026400.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM622"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM622_8"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            join(3042..3076,1..826)
                     /locus_tag="P4I30_RS30585"
     CDS             join(3042..3076,1..826)
                     /locus_tag="P4I30_RS30585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_276086061.1"
                     /translation="MPVTLRGKISSATEHGNMPEWARDEPAHFWQAADAFERANGSTYR
                     EIEIALPRELNEAQRLALVRDFVKQETGDKHAWTFAIHNPKASIDGGEQPHAHIMMSQR
                     VNDGIERSPEQYFRRYNAKFPARGGARKDSGHNLTPTEQKQALKALRERWERTHNAHMK
                     RYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGRLDDSQRQDIKDSRTFSVVDVPEQAK
                     HIDHSDRSWMNASGIEVNRKELVRSLTHDISQQYGREMAARVTKQGNVEIERVKSLSRG
                     FSISR"
                     /name="MobA/MobL family protein"
     gene            829..1359
                     /locus_tag="P4I30_RS30590"
                     /old_locus_tag="KAM622c_60090"
     CDS             829..1359
                     /locus_tag="P4I30_RS30590"
                     /old_locus_tag="KAM622c_60090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            complement(2434..2820)
                     /locus_tag="P4I30_RS30595"
                     /old_locus_tag="KAM622c_60100"
     CDS             complement(2434..2820)
                     /locus_tag="P4I30_RS30595"
                     /old_locus_tag="KAM622c_60100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     Typing          3..823
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.255"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 caccgaacac ggcaatatgc cggaatgggc gagagatgaa cccgcccatt tctggcaggc
       61 tgcggatgcg ttcgaacgtg ccaacggttc aacgtacagg gaaattgaga tcgctctacc
      121 ccgcgagctg aatgaagcgc agcggctggc gcttgtccgg gattttgtga agcaggaaac
      181 gggcgacaaa catgcatgga cgtttgcgat ccacaatccg aaagccagca ttgacggcgg
      241 cgaacagccg cacgcccata tcatgatgag ccagcgcgtc aatgacggta ttgagcgcag
      301 tcccgaacag tatttcaggc gctataacgc caaatttccg gcgcgtggcg gcgcgcgcaa
      361 ggactcaggg cataatttaa cccctacaga acaaaaacag gcactgaaag ccctgcggga
      421 gcgctgggag cgtacgcaca acgcgcatat gaaacgatat ggctttgagc agggttttat
      481 tgatagccgg tcactgaaaa cacagggtat cgaccggaaa ccggaacagc atctcggttt
      541 tgaagcagcc ggacgactgg acgacagcca gaggcaggat attaaagaca gccggacgtt
      601 ttcagtcgtg gatgtgcctg aacaggcgaa acatatcgac cacagcgacc gtagctggat
      661 gaatgccagc ggtatcgagg taaaccggaa ggaactggtc agatctctga cgcatgatat
      721 ctcgcagcag tacgggcggg agatggctgc acgggtcaca aaacagggaa atgttgaaat
      781 agagcgcgtg aaatccctgt cacgcggatt ttccataagc aggtaactat ggcgagcacc
      841 gaagaaaaac ttctcacact cctgctgtcg gcggagcatc tgaaagaggc cgcaaaagag
      901 cagcagaaag ccgtggcgga ttcgctggcg gtggcaaggg aaaccctgaa aggctaccgg
      961 gaaatatcgg acagtgtggc acagcaggta agggatgaag tccggcagga agtgcagaaa
     1021 atggatgttg cggggctgat tggcgagcgt atcagcgctg attttcagcg tctggaaaaa
     1081 tccgtcggga acatgaaaat caatgcggac gcgctggaca aggaaatttc cattatccgc
     1141 agtaacatca tcgcggagta caacacgctg cggggttaca gctggaagtg gtttgctggg
     1201 gttgcggttg cctttgtcgt actgatattt gccacaagct ggtttgtgaa aacggaacta
     1261 caggacaatt acaacgcgtc tgctgccgtc tatcagaaag tggcagacct tgaaaagctg
     1321 ctgacaacgg aacagaaacc ggaaaagaaa aagaactgac gcgctgcgct tgtccaacgc
     1381 ctcacgttcg cacaataaat tgcgcacccg ttcaccgttt tcagccgagt ttggggcgtc
     1441 ttttgagact tccggcctga attcaggccg gaagttctcg ccagtcagtc aggtgcatgg
     1501 ttatgcatgg ggctgaaaat cacggtatgc gattctgagc ggttttcggt gagaagccgg
     1561 gggtttttat ggctcaggac gtaaaaccgc cctgagcgcg tttcagcggt gcgcgtaacg
     1621 gatgttatgg taaatccggt ggctttgacg tgcctgccgt tgtgtaccgt cgtgagtgaa
     1681 aataattata ttgttcgaag aacagcgctt tttggccgga gaaaagcagt tctgcaaacg
     1741 atggtatatc gatatgagtt cttttggcat taatccaggc aagacgccgg gcaataagcg
     1801 taatattccg gatgttactt atttccattt ctgtcaaaat gcatcccccc ccacgccgga
     1861 gaataccata ttagggcaaa gaaagcctaa tgcccccgtg gtgggtacac cagaaaacgg
     1921 ggagcgaagc gaccagcatc aggccgttcg tatgctgctt cgattttgtg ggtgttgcgc
     1981 cagaccttgg caatcacgct tcaccgcaac cgctgccttc actctcactt tatcaggtac
     2041 gttattggtt aacgtgagac cgggtagagc cagcttcttt cccgagctgg tcaggttccc
     2101 accgcttgcc tgtttatcct gccggagtcc ggggcgtaga cctgagagtc aaggtgcaga
     2161 tatgggttta atgtcgttga atggtattaa gctggtagtg acattcctag ccagccctgc
     2221 taggatttga ttgtgacgtc tgatcctggc aggtatctgc aagaattaga gtactcaagc
     2281 cccgtgattc tgtcacgggg cttttgtaca tctggagcaa gaaaattcat gctcctatcc
     2341 gaaaatccct accgctcgcc gcagccgaac gaccgagcga cgcgagtcag tgagcgagga
     2401 agcggcattt ctccggcctt gcagaatgca cgttcacgcc gatgtttcca gatcgtagcg
     2461 tacccgtttc agcgcagtca gtaatttttc gtggtcaaaa ttcaactcac ccgtttttgt
     2521 atcaaccagt cccgcgtcaa tcatcagacc agccgccaga aacagtctgt gattgcgttc
     2581 cctgcggttt ttctgccctg aaatccgcgc ctgaaccgcc tttttctgtg cggcatcccg
     2641 ttcctgcaac gcgttttttt cagcggttgc cagaacgtgg agcagtcgtc tttcagtctc
     2701 actgagcgaa gcttcatcat ccagcagatc gataatctgc cgttgccgtg ccgttttttc
     2761 tcccagcttt ctgatgaagg ctatgcgctt tttaagccat tcgacgttac tgatccgcat
     2821 cgtttaactt ccccatcccg atatttccga cggggaaagt atataacttg cggtcagaag
     2881 tcgcatgact tatctttact cataattctt taaattagag caaagcgcac ttatatgttt
     2941 ctccctgcgg gcagaaacag tgcgccctgc cgggggctga cacaacatgg caacgtatca
     3001 tctcagcgtg aaatttggcg gcaagggtaa agcccttgct catgccagtt acattacgcg
     3061 ggaagataag ttcagc
//
