LOCUS       NZ_AP026399             4438 bp    DNA     circular CON 17-APR-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622
            plasmid pKAM622_7, complete sequence.
ACCESSION   NZ_AP026399
VERSION     NZ_AP026399.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446295
            Assembly: GCF_024347755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024347755.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 10:36:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,129
            CDSs (total)                      :: 6,017
            Genes (coding)                    :: 5,593
            CDSs (with protein)               :: 5,593
            Genes (RNA)                       :: 112
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 424
            CDSs (without protein)            :: 424
            Pseudo Genes (ambiguous residues) :: 0 of 424
            Pseudo Genes (frameshifted)       :: 118 of 424
            Pseudo Genes (incomplete)         :: 273 of 424
            Pseudo Genes (internal stop)      :: 123 of 424
            Pseudo Genes (multiple problems)  :: 78 of 424
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026399.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM622"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM622_7"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(95..304)
                     /locus_tag="P4I30_RS30545"
                     /old_locus_tag="KAM622c_60050"
     CDS             complement(95..304)
                     /locus_tag="P4I30_RS30545"
                     /old_locus_tag="KAM622c_60050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_118998865.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLNEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(308..793)
                     /locus_tag="P4I30_RS30550"
                     /old_locus_tag="KAM622c_60060"
     CDS             complement(308..793)
                     /locus_tag="P4I30_RS30550"
                     /old_locus_tag="KAM622c_60060"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_119002157.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYMTIREQKSTQAMLSERNSGVQLTTCGEQRRRCVRVNPEGGTFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<828..1508)
                     /locus_tag="P4I30_RS30805"
                     /pseudo=""
     CDS             complement(<828..1508)
                     /locus_tag="P4I30_RS30805"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1471..1794)
                     /locus_tag="P4I30_RS30560"
                     /old_locus_tag="KAM622c_60070"
     CDS             complement(1471..1794)
                     /locus_tag="P4I30_RS30560"
                     /old_locus_tag="KAM622c_60070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_198661910.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAVWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2434..2592
                     /locus_tag="P4I30_RS30565"
                     /old_locus_tag="KAM622c_60080"
     CDS             2434..2592
                     /locus_tag="P4I30_RS30565"
                     /old_locus_tag="KAM622c_60080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_118998265.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAAIGNWELVDLDI
                     ESLEVMA"
                     /name="replication initiation protein"
     gene            complement(2835..3101)
                     /locus_tag="P4I30_RS30570"
     CDS             complement(2835..3101)
                     /locus_tag="P4I30_RS30570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_341723485.1"
                     /translation="MICRFGGEEFVVFLPNTSVLTAECVAERIRSVIESTVFPNNLRVT
                     VSAGVATQDDPLGGIDFLLKNADDALYQAKGEGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     gene            complement(3041..3616)
                     /locus_tag="P4I30_RS30575"
     CDS             complement(3041..3616)
                     /locus_tag="P4I30_RS30575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_276086058.1"
                     /translation="MEWDFFKSDTEISIVSDDGTVIFNNNNSAVGRPMVMSDDLKSKLT
                     ESDRGEGDFFSSGNRYLLGYAHMKNTAWIGMLYMIALKTTTILITYAKSVLVLLIVVII
                     AFSLLSYFIASRISIPLEKLAISTNTKDIGASLVYIKAINAWYAEADRLKKALFTHIKV
                     MILVAVVKILFVGLVAKSLLFFCRIHLC"
                     /name="hypothetical protein"
     gene            complement(3691..4221)
                     /locus_tag="P4I30_RS30580"
     CDS             complement(3691..4221)
                     /locus_tag="P4I30_RS30580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="WP_276086059.1"
                     /translation="MGNYIEMAQSELAYGAKKIESTNDIDVLKNEADRLRLQSGMFNSV
                     LVVSNGAVVLATSPESLGLVGVHLNSSASKLAIEGKKPFVSAPYKSATGNLIVLLSHPI
                     YDKNGNYIGYVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNNNSAVGRP
                     NWEFDDLKSKLTV"
                     /name="PDC sensor domain-containing protein"
     Typing          complement(1904..2083)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.363"
                     /coverage="83.0"
                     /evalue="3.48e-51"
     Typing          2030..2546
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.101"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(7..1481)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.902"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tatgtccgct gccgctgcga tcgttcaagc tccagcgctt tctgatgctg cgccgcgcgc
       61 tcctgctccc ggtgtgcttc aatgccgtcc agtctcatga caaccggcgc agctgctcgt
      121 tcaaactctg cacctgcctg ctcaagcccg tgacgcgctc gctcagcgcc gcgttctccc
      181 gttgcataag accagacatc gtccgccatt ccgcgaaggc gttctcccac tcgtccagcc
      241 ttttcgagta gtcctgctgt agctgctcta atgcgctcag caactgtttt tccagctccg
      301 tcatgtgcta tttccccgcc agtatcatcc agctcgaatc ctctccgaac gtcccccctt
      361 ccgggttcac cctcacgcag cggcgtctct gctctccgca ggtcgtgagc tgcacgccgc
      421 tgttcctctc ggacagcatg gcctgcgtgc tcttctgctc ccggatggtc atgtaattgt
      481 cgagtatctg ctgcccctgc caccacagaa tgcccgcgct cgaggcgatc agcagcgcgg
      541 agaccagcac gatggtcagc cacgtctggc tgaccatgcg cagcatccct ttcgttcgct
      601 ggctcatggc tgacgacagc ttccggtcgt ggtcgaggat ggcggcgctg attctcttct
      661 cgctttcgct cagttccgct ctgacagact tctcgtgctc gctgaatgcg gctttcagca
      721 tctcgccggt agtctgctgc tgcgctttcg atttctgctc taagtccttc gccagcgtta
      781 aaagactgtt catagatggc tcccttcagt cggatgtttc gccccccgtc cgggtcggca
      841 atgctgatgc tgctttttgt ggtgcgcacc acttcaaaac ccgcgctttc cagcgcctca
      901 gtgacgtcct gacgcgtttt cagctccccg gacgaggcta aggccagtaa accccgcgtg
      961 atcgcctgag ctgcctcctg cttcgcttcc ggcagcgcgg aaggcgtgac cagcgcccgc
     1021 cggttttccg gcgcgttcgg gtcgtgcagc cccatcctgc cgttcacgat ggtctgccag
     1081 gcatcgatgc gcggacggtc agcgcggtcg taatacggct ggagccgttt gccggtcagc
     1141 agctccgtgt tcggtaccag aaaattaagc tccagacggc ccttgtcctg gtgttcaacc
     1201 cacagcacgc tgtactggtc tttatcgagt ccgggcatca gaacccgctc gaaactcgcc
     1261 atcagcgttt cacgctggcc gggcggtaag tcctgttcgg caaaggacag gacgccggag
     1321 gtgtactttt ttgcataagg cgaggcgtcg ataagctccc ggacttcctc cggcttcccc
     1381 tgcagaacgc tggcaccttc gcgctggcgg tcttttccca gcagataatc caccggaccg
     1441 gcgccgccgc cacgtccccg aggatgaaac ttaacgatca tcgtctgcac ccttttccag
     1501 cacggcatgc ctcagccgct cgagtccggc atcgattgcc atcagcgcgg cgacgacctg
     1561 tacccggtcg tgcccgctgc cgccaccggc gttaacctgc cgggcaatct gattgaggtt
     1621 gttccccatg ccggcaagct gacgaagcag cgccggcgag agcgacggaa gtttgccggc
     1681 acgagcgggc ttctcgtcca ggcacgtctg ccgcatccag accgcgagct gcttaccgtc
     1741 gcagcgctcc agcagacggc ggtgctcatc ctcggtaacc cacatcgtga gcattttgct
     1801 gcgtttatct gccaatccgg cctcctgata acgccgggac gggcgggaaa tcttttcccg
     1861 gtcggcgcgg cgttggacaa gccgcaggcg cgtcagtggc ttttagcggg tttcggggcg
     1921 cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggcttacta tgataaatcg
     1981 gagtggtgat tcagggaaat gctttatgta gcagggggaa aatgcagctg cggggcgtaa
     2041 gtgcaaaatg caggtggcga tgaaatgacg cctcctcgct cactcggtcg ctgcgctcct
     2101 gccgtgagac tgcggcgggc gttacggact tacaaataat gggagagcag atggcgagaa
     2161 gtcaggaaga ccagaagaag gatgcggcgt tgccgttttt ccataggctc cgcccccctg
     2221 acaagcatca cgaaatctga cgctcaaatc agtggcggcg aaacccgaca ggactataaa
     2281 gatcccaggc gtttccccct ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt
     2341 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
     2401 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     2461 tgctgcgctt tatccggtaa ctatcgtctt gagtccaacc cggaaagaca cgccaaagcg
     2521 ccactggcag cagccattgg taactgggaa ttagtggatt tagatatcga gagtcttgaa
     2581 gtgatggcct gactacggct acactgaaag gacagtattt ggtatctgcg ctccacttaa
     2641 gccagttacc ttcggaagaa agagttggta gctctgaatt ccggcaaacg aaccaccgtt
     2701 ggtagcggtg gtttttttgt ttacagggca tgagcttacg acgacatcga agggataaca
     2761 agaggatccg cttcttgaac cctcttcccc acaaaaaaaa cgacacacta gcgcgtaaag
     2821 cgtcatcaaa aaactcaaat agcgctatac acaatcactt tattacgccc ctctcctttg
     2881 gcttgataaa gagcatcgtc agcatttttc agcaaaaaat cgatcccacc aagaggatca
     2941 tcttgtgttg ccacaccagc agatacggtg acccgcaagt tattagggaa aacagtactt
     3001 tcaataacac tcctaatacg ctccgcaaca cactctgctg ttaacacaga tgtattcggc
     3061 agaaaaacaa caaactcttc gccaccaaac cgacaaataa tatcttcact acggcaacaa
     3121 gaatcattac cttaatatgc gtaaaaagtg cctttttaag tcgatcagct tcggcatacc
     3181 aagcattgat agctttaata taaactagcg aagctcctat atctttagta ttggtagata
     3241 tagcaagctt ttctaaagga attgaaatac gggaggcaat gaaatatgaa agaagtgaaa
     3301 atgcaataat tactacaatt agcagaacaa gtacactttt agcataagta ataagaatag
     3361 tagtcgtttt taaagcgatc atataaagca taccaatcca agcagtattt ttcatatgag
     3421 cataacccaa aagatatcta ttccccgaag aaaaaaagtc cccctcacct ctatcagact
     3481 cagttaactt tgatttcaaa tcatcagaca taaccatggg ccgaccaaca gcactgttat
     3541 tgttattaaa gatcacagtt ccatcatcac taacaatgct aatttcagtg tcgctcttga
     3601 aaaaatccca ttccaagcag tatttttcat atgagcataa cccaaaagat atctattccc
     3661 cgaaaaaaaa aagtccccct cacattctta tcatacagtt aactttgatt tcaaatcatc
     3721 aaattcccag ttgggccgac caacagcact gttattgtta ttaaagatca cagttccatc
     3781 atcactaaca atgctaattt cagtgtcgct cttgaaaaaa tgcctgctca aaacatcgct
     3841 aaacaaacta tgtttcttta gatatataga cccgccgaca taccctatgt aattgccatt
     3901 cttatcataa ataggatgtg acaacaaaac aatcaaattc ccagtagctg atttgtaagg
     3961 tgccgatacg aatggttttt tgccctcaat cgccaatttg ctggcacttg aattcaagtg
     4021 gacaccaact aaaccaagtg attctggcga agtcgccaga acaacagctc cattactaac
     4081 aacaagaact gaattaaaca taccagactg caatcttaac ctatccgcct catttttaag
     4141 tacatcaatg tcattagttg attctatttt ttttgcacca tatgccaatt cactttgagc
     4201 catttcaata taattaccca ttacatctga cattttcata gcgtacgatg aattagcatc
     4261 aagcaagtct tattctatat tttcgcgctg aaaaaccatc ccgaaaacca tccattctgc
     4321 acctgataaa tttcaccgga ttgcatagcc atgcagcagg gtgaatggca ccggctttcg
     4381 gcgcagatac ccgccggaag cctcgtttta tccgaattgt tacagcgacg gtccattg
//
