LOCUS       NZ_AP026391             1853 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter braakii strain KAM621 plasmid pKAM621_9, complete
            sequence.
ACCESSION   NZ_AP026391
VERSION     NZ_AP026391.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446294
            Assembly: GCF_024347735.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Citrobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 112x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:27:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,612
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 88 of 228
            Pseudo Genes (incomplete)         :: 146 of 228
            Pseudo Genes (internal stop)      :: 40 of 228
            Pseudo Genes (multiple problems)  :: 41 of 228
            Pseudo Genes (short protein)      :: 1 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026391.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="KAM621"
                     /db_xref="taxon:57706"
                     /plasmid="pKAM621_9"
                     /geo_loc_name="Japan"
                     /collection_date="2018"
     gene            join(1768..1853,1..820)
                     /locus_tag="V0Z23_RS28055"
                     /old_locus_tag="KAM621c_55160"
     CDS             join(1768..1853,1..820)
                     /locus_tag="V0Z23_RS28055"
                     /old_locus_tag="KAM621c_55160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_326978239.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVWSTLKETGETRLRLRE
                     AHFCRVRHCPVCQWRRSLMWQARFYQSLPRIVADYPDARWMFLTLTVRNCAIGELGETL
                     NRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGSDGSAHPHFHTLMMVPPSMFTRDYVKHD
                     RWVELWRECLRVNYDPNVDVRAVKPRKPKDGESLASATAELVRGAVAETLKYSTKPADM
                     VADPEWFLELTKQTHKRRFVATGGALKDILKLDQETDADMVIGDDIPEGDDDGSRIAFE
                     WKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     gene            1479..1781
                     /locus_tag="V0Z23_RS28060"
     CDS             1479..1781
                     /locus_tag="V0Z23_RS28060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     ARG             1769..1851
                     /name="PEDO-3"
                     /gene="PEDO-3"
                     /gene_name="subclass B1 PEDO beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem"
                     /identity="100.0"
                     /coverage="11.81"
     Typing          complement(547..735)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 ttggtctacc ctcaaagaaa ctggagaaac tcgccttagg ctacgtgagg cgcatttctg
       61 ccgtgtccgg cactgtcctg tctgccagtg gaggcgatcc ctgatgtggc aagcccgttt
      121 ttatcagtcc ctgccgcgta ttgttgcgga ttatccagat gcacgctgga tgttcctgac
      181 cctcacagtg cgaaactgcg cgatcgggga attgggtgag acacttaacc gaatgaacat
      241 ggccttccag cgacttaagg atcgcaaaga gtttcgacct gttcagggct ggatccgcac
      301 cacggaagtt acacgtggaa gtgacggtag tgcgcacccg cactttcaca ctctgatgat
      361 ggttcctccc tcaatgttca cgcgagatta cgttaaacat gaccgctggg tagagctttg
      421 gcgagagtgt ttgcgtgtga attacgatcc gaatgttgat gttcgagcag taaaaccccg
      481 caagcctaag gacggagaat cattggcttc tgcaactgcg gaacttgtac ggggcgcagt
      541 ggctgaaacc ctgaaatatt ccacgaagcc ggctgacatg gttgctgatc ctgaatggtt
      601 cctcgaattg actaagcaga cacataagcg ccgatttgtg gctaccggtg gcgcactcaa
      661 agacatactg aaactcgatc aggaaactga tgccgatatg gtaatcgggg atgatattcc
      721 cgaaggcgat gatgatggtt cacgcattgc ttttgagtgg aaaaccgaat ccaaaaagta
      781 ccgtcgctca ccgtcaaaag ataaagctga atcagactga cagggtgtcg gggcgcagcc
      841 ctgaccacga cgtttttgag aggacgccgc ttgtcggcgg tactccaaaa acacatcttg
      901 tcctattggt tctactccga tgcaggatag ggctaacgcc gcaaagtgac gtcactcaat
      961 tactgagttc ggtttccgaa ctcagttggg acaagatgtg tttttggtac accgccatca
     1021 cgatgtcggt gccaaaaacg attcgtcagg gctgcgcccc gacaccctgc caacacctca
     1081 cgcagtcaga ttaaaatctg ccagcgctcg ccgtttccga aatcgtcaaa ctctaaatcg
     1141 tggggtcccg ctggcttgcc agtgggttcc gatttagtga ttgacgacat tgctgattga
     1201 aaaattggct gaggtggttg gattcacgaa gtgaggccaa ccacctggaa gacggttttt
     1261 aattgtctgg taccggatac aaccagaacg aatggagttg gttctgtcgt gatgcgaaaa
     1321 agtatgggtt cgcaaatcac agactaaata gatttaccca attccattcg agtgaggttg
     1381 ttatgttttt tgaggttctt aatcatgttg atagtgcgtt gagcgttgct gtttcgctcc
     1441 tggcgttgtg gcagtttttg gtggcgggtg ggcgttgaat ggctggttcg atccgcgcgc
     1501 gatccttgta tttatactta agggataact ctggcgataa aaaatggcat caagccttgc
     1561 gtaccaaggc ttcggggctt atttgcgttg tagaaggttt tggaagtatt ctgttgggag
     1621 tggtcgcctt aaaagcgacc cccgcaacga cggccatcgt tcgggggtgt gaccacctgc
     1681 agaaattcga ttcgaagcga caaccaacag gtgatacggc aatgataaca cgtcaaatga
     1741 gccatcgccc agcatgggac ggtgtgcatg caaaacccta aactccagaa cctcacggat
     1801 tactccccct cagacgcgcc ctgggacgct cacaaaagcg tttctgacga cgt
//
