LOCUS       NZ_AP026390             2705 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter braakii strain KAM621 plasmid pKAM621_8, complete
            sequence.
ACCESSION   NZ_AP026390
VERSION     NZ_AP026390.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446294
            Assembly: GCF_024347735.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Citrobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 112x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:27:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,612
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 88 of 228
            Pseudo Genes (incomplete)         :: 146 of 228
            Pseudo Genes (internal stop)      :: 40 of 228
            Pseudo Genes (multiple problems)  :: 41 of 228
            Pseudo Genes (short protein)      :: 1 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026390.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="KAM621"
                     /db_xref="taxon:57706"
                     /plasmid="pKAM621_8"
                     /geo_loc_name="Japan"
                     /collection_date="2018"
     gene            join(2580..2705,1..3)
                     /locus_tag="V0Z23_RS28030"
     CDS             join(2580..2705,1..3)
                     /locus_tag="V0Z23_RS28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_326978238.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDLTPLAAAIGKRIHGA"
                     /name="replication initiation protein"
     gene            <270..371
                     /locus_tag="V0Z23_RS28035"
                     /pseudo=""
     CDS             <270..371
                     /locus_tag="V0Z23_RS28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            complement(560..934)
                     /locus_tag="V0Z23_RS28040"
                     /old_locus_tag="KAM621c_55130"
     CDS             complement(560..934)
                     /locus_tag="V0Z23_RS28040"
                     /old_locus_tag="KAM621c_55130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061335866.1"
                     /translation="MNGMSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSL
                     LGQGFSLPGGDKDTHSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKE
                     LNPDFTTYEIGRFLFKHLQG"
                     /name="hypothetical protein"
     gene            1263..1451
                     /locus_tag="V0Z23_RS28045"
                     /old_locus_tag="KAM621c_55140"
     CDS             1263..1451
                     /locus_tag="V0Z23_RS28045"
                     /old_locus_tag="KAM621c_55140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1499..1699)
                     /locus_tag="V0Z23_RS28050"
                     /old_locus_tag="KAM621c_55150"
     CDS             complement(1499..1699)
                     /locus_tag="V0Z23_RS28050"
                     /old_locus_tag="KAM621c_55150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080484700.1"
                     /translation="MNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRHIVSDTNQAR
                     RACMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     Typing          2200..2695
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.97"
                     /coverage="82.0"
                     /evalue="0.0"
ORIGIN
        1 tgactgcggc tacactggaa ggacagtttt ggtatctgcg ctccgtgaaa gccagttacc
       61 ttcggaagaa agagttggta gctcttgatt ccggcaaacg aaccaccgtt ggtagcggtg
      121 gtttttttgt ttacaggaca tgagattacg acgagagtaa aaggatcaca agaagatccg
      181 gctgtttacc agtgatttgg cttcagtatt ggtttccgcc tagcggggcc ttatacgtaa
      241 aaattccaca cctgacttat cacacagggg agctccgcaa gtaccagaga gcactgaata
      301 gcaggccacg gaaatgtctt ggattccggc aaccttcagt ggtctttgct gagctaagac
      361 aggcggctta gcgttgcact tcgaacttga atccgcccag aagcattcag cagaagtcgc
      421 taacggtttg cttgcaaatg ctatcaataa tgctttgcct aaagcaggaa taaaaaagcc
      481 acctacacgg tggctttttt tctgtccggg aactagctga attgaaccta aaatcccggc
      541 cacgaactgg tagacacctt tatccctgaa gatgtttgaa gagaaaacgg cctatttcat
      601 aggtcgtaaa gtcagggttt agctctttat catccatccc tacagcgctc cctgcctcga
      661 tcaatacctt cttcatgttg ctaactttaa gcccgtcttt ggtaaccaag atctttcctt
      721 ttggaagctt atgaatcacg taatccttga aggaatgtgt gtccttatct ccaccaggca
      781 aagaaaagcc ctgacctaat aacgatttta atgaattatt ctcttcaatt aagttcagga
      841 caacatcatc aaccgtcatg ccaaagtcat tcacgtactc ttttaggctc tcaatagttg
      901 atttctttag tcttacgtca gacataccat tcatattatt attaccccac cgctctaatt
      961 tgaagaaata atacaacaaa atacaaatta tacaaataaa tattgttctg aatacaacaa
     1021 gcggtctgta ggtgcgatga taactatgcc gcaccgccag attgaacata acatccgttg
     1081 cgtgcaccgc ctgagaggct ctcagggcgt ttctacgacc tcacctgcaa aagaacacat
     1141 cttaccccct gaaaaccgct cagaaacgcg ctgatgattt ctgcgtacct gcatggctat
     1201 gcactcaagt gaatgaagta agatttcagt ctgcgatcac cataaaaccg ggaaaaagcc
     1261 gtatgacaaa acaggaatca gccgcactga acatggcaaa atttatccgg gcgcagtcgc
     1321 tgctgctgct cgaaaaactc gacgtgctcg atcttgatga cgaggcagcc gactgtgagc
     1381 gaatgcacga acaggcagaa gcgctttatc agaagctgag cgcacgcttc ggtatacagg
     1441 acggcgaata aagagacgaa aagaacgctc acttagacga aaagcgtatc cagggcaact
     1501 atgtatatcc accgtgtaca tagtcatgcc tgacgctttc gagcaggtcg agcatgcagg
     1561 cacgacgagc ttgattggta tcggacacaa tatggcgata cgcctcaaac ataacagcac
     1621 ccgcatcacc gcccgaacgg cgaagcatag cggaaatcat ccgcacaaag agcagacgtt
     1681 catcggggca aaggttcata agttctcctg gtgaaaaacc aaacatgaaa gcacccatct
     1741 attaacgtca gctgtgaacg cttcattaga agaaaatagg taaacccggg aaaaagatcc
     1801 aaacgttaac atcacaggta aacagaaacg tcgggtcgat cgggaaattc tttcccggac
     1861 ggcgcggggt tgggcaagcc gcaggcgcgt cagtgctttt agcgggtgtc ggggcgcagc
     1921 cctgaaccag tcacgtagcg ctagcggagt gtatactggc taaacttcag agatgccaag
     1981 gaagggacag gatatgtttt gtagcaccgc caccacggtg gcgatactcc aaaacgcctg
     2041 gtcagggcac agccccgaca ccccagagcc gactgtaggt cactggacga acgcggcgga
     2101 atgatgtcgg atgacgtcat tttgcggcgt ttgccctatc ctgcatcgta ttaaaaaatg
     2161 cagctatggc gcgtacggtg ccaaagataa gcccggagat attccgcttc ctcgctcact
     2221 gactcgctcc gctcggtcgt tcggctgcgg cgagcggtac cggcttactg gcggggcgga
     2281 aatttcctgg catcttccag gaagaagctt aacagggaag caagaaggcc acggagaagc
     2341 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
     2401 ggtggcgaaa cccgacagga cttaaagata ccaggcgttt ccccctggtg gctccctcgt
     2461 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     2521 atctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     2581 tgcacgaacc ccccgttcag tccgaccgct gcgccttgtc cggtaactat cgtcttgagt
     2641 ccaacccgga aagacacgac ttaacgccat tggcagcagc cattggtaag aggattcacg
     2701 gagca
//
