LOCUS       NZ_AP026389             3819 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter braakii strain KAM621 plasmid pKAM621_7, complete
            sequence.
ACCESSION   NZ_AP026389
VERSION     NZ_AP026389.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446294
            Assembly: GCF_024347735.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Citrobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 112x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:27:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,612
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 88 of 228
            Pseudo Genes (incomplete)         :: 146 of 228
            Pseudo Genes (internal stop)      :: 40 of 228
            Pseudo Genes (multiple problems)  :: 41 of 228
            Pseudo Genes (short protein)      :: 1 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026389.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="KAM621"
                     /db_xref="taxon:57706"
                     /plasmid="pKAM621_7"
                     /geo_loc_name="Japan"
                     /collection_date="2018"
     gene            join(3120..3819,1..317)
                     /locus_tag="V0Z23_RS28010"
     CDS             join(3120..3819,1..317)
                     /locus_tag="V0Z23_RS28010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_326978237.1"
                     /translation="MIHKHIGGKKNQRSVKNSLDYLLRTKKPEEQKFVEVLSQTNRSDI
                     ENFNEYIKDKNFKNPFITGVLSFEEKDIDPELKTKIMNDFEDMMFSGIEPENRPPVIWI
                     QHKDKNRLELNYTTFNSLMNKKHFQCYYHLSDKKLFNSFCEKINYENNISSVLDVQEHK
                     ARNTLVNTLNNKIPEEKKAVVEKLQEEILSKIITEEINNREELISFIQSKNIIINRVRE
                     NAISIKFDKADNMNRTEITKLIEQNQQSITEQISSMSKTNSDTIEKILTQSLKKDTAEI
                     ELVLSQHRAKIRKMLNTELKKQMTVKMLDLKYQTIIMILLGMNLILIFFIIIKLSRM"
                     /name="hypothetical protein"
     gene            321..758
                     /locus_tag="V0Z23_RS28015"
     CDS             321..758
                     /locus_tag="V0Z23_RS28015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032645814.1"
                     /translation="MSKISQMIKQNQEKKNQQNLIYQEKLKQLIHNNNSFVQELKRLEE
                     INSNSESRLIELRNDLEYVEDLYIQLFTILEDTSERFQSIQDRMKEDKKSNTDKKSSLK
                     SSKPSKKKTIKEVSSEVDLNRIRTVLNTLLTHYDKTLTVNK"
                     /name="hypothetical protein"
     gene            1098..2501
                     /locus_tag="V0Z23_RS28020"
                     /old_locus_tag="KAM621c_55110"
     CDS             1098..2501
                     /locus_tag="V0Z23_RS28020"
                     /old_locus_tag="KAM621c_55110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127713719.1"
                     /translation="MSEKSIEEMLAEKQIKEFNESKFGQFFNNKKLKEQELTYLNNKAL
                     DIVNEEDLFEIMSLIPQPFITDENKNVDVLSLIYNNFLEHTEIPLTLPIFSFLSFLSAF
                     CVKNEITYTIPLSDEKSPLDTWITILAPSGSGKSFSNSQMKKVIPTGIDGKKVIEPNFV
                     KPNGPAKFIEELSNLPTTKNGENQFGFWIEDEVSQMFKQVEQIGSPLSEIKEYLLKAYD
                     HEKLTRKSKKESIETKGIILTLLFINTFESYANNISNESMTDGLMRRFNLIYTEKDIER
                     DFTDYYLYNLKKIRDDRLQDEIADLFFSIKPDQNFTFSDNCFKLYGKYFKIFWHRKYKN
                     ILTDKENFYRTYMMQSWKFAVFYHLILKREGTEIQGDCLIYGMKISLLFANSMKKFFDF
                     KIRKNVPDKKIEKIKNDLTKYIVFLENNPEATLRDFCRKYNIRKIDANSILKSLQCANI
                     QHKLIEELE"
                     /name="hypothetical protein"
     gene            2783..3130
                     /gene="mobC"
                     /locus_tag="V0Z23_RS28025"
                     /old_locus_tag="KAM621c_55120"
     CDS             2783..3130
                     /gene="mobC"
                     /locus_tag="V0Z23_RS28025"
                     /old_locus_tag="KAM621c_55120"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_048982277.1"
                     /translation="MSYRERYQRKNFISLCLSDEELSEIENIADRLNMKRAAAAREILV
                     TNSKRLKSQIKKNDNSEILFLYSKISNNINQIAKKMNTNLDKFLSGNGEEFSLLIEEIF
                     EDLERLKNNDT"
                     /name="mobC"
ORIGIN
        1 atatgaacag aacagagatt acaaaactta tagaacagaa tcaacaatcg atcacagagc
       61 agatcagcag tatgtcgaaa accaactcag acaccataga gaaaattttg acacagagct
      121 taaaaaaaga cacagcagaa atagaactcg ttttaagcca acacagagca aagatcagaa
      181 aaatgttgaa tacagagtta aaaaagcaaa tgacagtaaa aatgttagat ctgaaatacc
      241 agacaataat aatgatcctg ttaggaatga acctgatatt gatttttttc ataataataa
      301 aactatcgag gatgtaagca atgagcaaaa tttcacaaat gataaaacaa aaccaagaga
      361 agaagaacca gcaaaatttg atctatcaag agaaattgaa acagctaata cacaacaaca
      421 attcattcgt tcaagagttg aagaggttag aagaaatcaa cagcaattca gaaagtcgct
      481 tgatagaact caggaacgat ctggagtatg tagaagatct ctacattcaa ttattcacaa
      541 tattagaaga tacgtcagaa cgttttcaat ccatacaaga cagaatgaaa gaagacaaaa
      601 aatcgaacac agacaaaaaa tcttccttga aaagctcaaa acccagcaag aaaaaaacaa
      661 tcaaagaagt gtcgtcagaa gtcgatttaa accgtattag aaccgttctg aacactcttc
      721 taacccacta tgacaaaacc ttaaccgtga acaagtgaaa gccccacata gtggggtttt
      781 tgcttgtgag gggttggcct gccggcgagg cggttttgtc gtttgttttt ttatttttta
      841 tgacaggtaa aaaataaata tgtttcaaaa tatttcaaaa actccaagtg atttgatttt
      901 gtcaaaactt tgtcaaagat ttgtcaaaac caaaaaaaat ggtttttcct ttaaaaacaa
      961 aggctttatt gatttgtcaa agtgtcatag tgtcaaaaag aagcgcgaaa aaaaagtcat
     1021 atttttttta aaaaatattt gacacattta aaaatgtatt atatatattt tatataatca
     1081 aaataggaga attaattatg agtgaaaaat caattgaaga aatgttagct gaaaagcaaa
     1141 taaaagaatt taacgaatca aaatttggtc agttttttaa taataaaaaa ctaaaagaac
     1201 aagaactaac ataccttaac aataaagcat tagacatcgt aaatgaagaa gacttattcg
     1261 aaataatgtc attaatacct caacctttta ttactgatga aaataaaaat gtagatgtat
     1321 taagtcttat atataataat tttttagaac atacagaaat accattaaca cttcccatct
     1381 tttcgttttt aagtttttta agtgcttttt gtgttaaaaa tgaaatcaca tatacaattc
     1441 cattgagcga cgaaaaatcg ccgcttgata cttggatcac aattttagcc ccatcaggta
     1501 gcggaaaatc attctcaaat tcacaaatga aaaaggttat tcctactgga atagatggaa
     1561 aaaaagttat tgaaccgaac tttgtaaaac ctaacggtcc cgcaaaattt atcgaagaac
     1621 tttcaaattt acccacaaca aaaaatggcg aaaatcaatt cggattttgg attgaagatg
     1681 aagtttcaca aatgttcaaa caagtagaac aaattggatc tccgctctct gaaattaaag
     1741 aatatttatt aaaagcatat gaccatgaaa aattaaccag aaaaagcaaa aaagaatcaa
     1801 tagaaaccaa aggcattatt ttaactttat tatttataaa tacatttgaa agttatgcga
     1861 ataacatttc aaatgaatct atgacagacg gattaatgcg tagatttaat ttaatataca
     1921 cagaaaaaga tattgaacga gatttcactg attattattt atataattta aagaaaataa
     1981 gagatgatag attacaagat gagattgctg atttgttttt ttctataaaa ccagatcaaa
     2041 atttcacctt ttctgataat tgttttaaat tatatggcaa gtattttaaa attttttggc
     2101 atagaaaata taaaaatata ctcacagata aagaaaattt ttatagaact tatatgatgc
     2161 aaagctggaa gtttgctgta ttttatcatt taattttaaa aagagaagga actgaaattc
     2221 agggtgattg tttaatatat gggatgaaaa tatcattact ttttgctaac agtatgaaaa
     2281 agttttttga ctttaaaata agaaaaaacg ttccagataa aaaaatagaa aaaataaaaa
     2341 atgatttaac aaaatatatt gtttttttag aaaataaccc agaagctaca ttgagagatt
     2401 tttgcagaaa atataacatc agaaaaatag atgctaattc tatattaaaa agtcttcaat
     2461 gtgctaatat tcaacacaaa cttattgagg aactggagta attaacaagc gaattttaaa
     2521 ttgtgccata ttcaaatata ttttcgcttg ttcaacccct caaaaataat aaagccctga
     2581 cgggcttttt tattttctca cggttttata aaaggtcaaa ttatattcag ttttgccctt
     2641 gacttaatgt tttaatttgt tatctatgta atagatagca agttagagta atacttcgta
     2701 ttcctttact tgtgaggggt tcccccttca atcccctaaa aaccaaatca aaatcaaaac
     2761 cttaaaccca aaggagttaa atttgagtta ccgtgaacga taccagagaa aaaattttat
     2821 ctcattgtgt ttgagtgatg aagaattatc agagattgaa aatattgctg ataggttaaa
     2881 tatgaaacga gcagccgcag ctcgtgaaat attagtcaca aactcaaaga gactaaaatc
     2941 acaaataaag aaaaatgata attctgaaat attgttttta tattctaaaa tctcaaacaa
     3001 tataaatcag atcgcaaaaa agatgaacac taatcttgat aaattcttat caggaaacgg
     3061 agaagaattt tcactactca ttgaagaaat attcgaagat ttagagaggt taaaaaataa
     3121 tgatacataa acatatcgga ggaaagaaaa atcagcgaag tgttaaaaat tcacttgatt
     3181 atttattaag aactaaaaaa ccagaagaac agaaatttgt tgaagtttta tctcaaacaa
     3241 acagatctga tattgaaaat ttcaacgaat acataaaaga caaaaacttt aaaaacccct
     3301 tcataactgg tgttttatct tttgaagaaa aagacatcga tccagaatta aaaacaaaaa
     3361 taatgaatga ttttgaagat atgatgtttt caggaataga accagaaaac agacctccgg
     3421 tcatttggat acaacacaaa gataaaaaca gacttgaatt aaactataca acttttaatt
     3481 ccctaatgaa taaaaaacat tttcaatgtt attatcattt gtcagataaa aaattattca
     3541 actcattttg cgaaaaaata aactatgaaa ataatatcag ttctgttttg gatgttcagg
     3601 aacataaagc aagaaatacc ctcgttaaca ctttaaataa taaaatacca gaagaaaaaa
     3661 aggcagttgt tgagaaatta caagaagaga ttttatcgaa aataatcaca gaagaaataa
     3721 acaacagaga agaattaata tcatttattc aatctaaaaa cataataata aacagggtta
     3781 gagaaaatgc tatatcaata aaatttgata aggctgata
//
