LOCUS       NZ_AP026387             4873 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter braakii strain KAM621 plasmid pKAM621_5, complete
            sequence.
ACCESSION   NZ_AP026387
VERSION     NZ_AP026387.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446294
            Assembly: GCF_024347735.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Citrobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 112x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:27:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,612
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 88 of 228
            Pseudo Genes (incomplete)         :: 146 of 228
            Pseudo Genes (internal stop)      :: 40 of 228
            Pseudo Genes (multiple problems)  :: 41 of 228
            Pseudo Genes (short protein)      :: 1 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026387.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="KAM621"
                     /db_xref="taxon:57706"
                     /plasmid="pKAM621_5"
                     /geo_loc_name="Japan"
                     /collection_date="2018"
     gene            complement(join(4515..4873,1..718))
                     /locus_tag="V0Z23_RS27940"
     CDS             complement(join(4515..4873,1..718))
                     /locus_tag="V0Z23_RS27940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_326978235.1"
                     /translation="MAIYHLSFSILKRSDGKSSCYLSAYNTRSKIVDNRTGDVWDYTKK
                     SGLDYHEVLLPKNGDKKYLDPAVLWNHLEDIEKRKDAQLSKYFDLAIPVELSREEKINL
                     VKEYCHQNFVSKNIVCDISFHDLESDNPHAHVMTTLRPMINGKFDKKLRNFQDKDLINR
                     HRENWADIANKYLTPYNKKISHKTLDNQRIEHLEKSEECFSKNEPISAVAHLIIAEGLD
                     RDPMFRINRKFWNTPEAQKFDEAKAFASNFVDIKNIAKKAGTFEVFKNWMKKSLLEIFD
                     NIRKQNKKKPLYNKQAFTFKTLDQRKEEVLNNASKTPSIASRDDLATKLSKHIAYNKKI
                     AQEASEQVDLKTGLPKLK"
                     /name="MobA/MobL family protein"
     gene            1037..1312
                     /locus_tag="V0Z23_RS27945"
                     /old_locus_tag="KAM621c_54970"
     CDS             1037..1312
                     /locus_tag="V0Z23_RS27945"
                     /old_locus_tag="KAM621c_54970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080917370.1"
                     /translation="MDYLIFEIRNMTKYEMKLFIEALKEDQDKKEFFELFKKYYVKTNY
                     SSGKNLFKTERKEQRIANYKIARLLNTIKTINIDNIREAFYVGETK"
                     /name="hypothetical protein"
     gene            1293..1577
                     /locus_tag="V0Z23_RS27950"
                     /old_locus_tag="KAM621c_54980"
     CDS             1293..1577
                     /locus_tag="V0Z23_RS27950"
                     /old_locus_tag="KAM621c_54980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080917373.1"
                     /translation="MSEKLNKKTLDQNINKLELELQKLKSKKKNIRRKEIEHKKFVLGG
                     LVAKYFSVDVDLEFIEDLLKQASEQLNGTNKRINEENLNSNDIAWKDLE"
                     /name="hypothetical protein"
     gene            1574..2125
                     /locus_tag="V0Z23_RS27955"
                     /old_locus_tag="KAM621c_54990"
     CDS             1574..2125
                     /locus_tag="V0Z23_RS27955"
                     /old_locus_tag="KAM621c_54990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142485371.1"
                     /translation="MKKRYKFLYFDFVKKKNENEYEFKQSKEQIKKFKDDYLINITSHI
                     DLCIPKESEDRVSYFAKFAQLLGANTKRFRSELSYALCSVFFEIYEYDKTYHSAQNFIF
                     DLTGKKLTREIALKEFSNVDNLSGNFSRSESSIFHKKNKQKVEENKKEFLSVFPYKSIS
                     YSMDSVINEAKELVKNKAKK"
                     /name="hypothetical protein"
     gene            2291..2608
                     /locus_tag="V0Z23_RS27960"
                     /old_locus_tag="KAM621c_55000"
     CDS             2291..2608
                     /locus_tag="V0Z23_RS27960"
                     /old_locus_tag="KAM621c_55000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080917355.1"
                     /translation="MAISNIDRMALLKEEMKLLRKQQKEEKQKKVENLIFMIGQSLVEQ
                     SGINAKKEEEISSLLYYIKNIENKPEGFVFDKTKFEKHLDKKPTPRKTTNTTTKPQQKT
                     E"
                     /name="hypothetical protein"
     gene            2689..3021
                     /locus_tag="V0Z23_RS27965"
                     /old_locus_tag="KAM621c_55010"
     CDS             2689..3021
                     /locus_tag="V0Z23_RS27965"
                     /old_locus_tag="KAM621c_55010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142257174.1"
                     /translation="MDKQSIMNLIDDLNVGKGFLIAIGDLYYQTDNTELVSSFLKSKID
                     EVYTNYFRDVFVDLSNQEFKDHAFYVAIDNLLKLKTKNIENIREILAIVSAIDEMIEDY
                     KQALDI"
                     /name="hypothetical protein"
     gene            complement(3070..3336)
                     /locus_tag="V0Z23_RS27970"
                     /old_locus_tag="KAM621c_55020"
     CDS             complement(3070..3336)
                     /locus_tag="V0Z23_RS27970"
                     /old_locus_tag="KAM621c_55020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="WP_142485369.1"
                     /translation="MTAKIQRKRNMSAKEAAEIKGCHVRTVKRYIAQSREDYEQEAQEK
                     RSLAFHLRSSGLKWREVAEKMNTTENAAIAYYRRYLALDLKTH"
                     /name="plasmid replication protein"
     gene            complement(3340..4197)
                     /locus_tag="V0Z23_RS27975"
                     /old_locus_tag="KAM621c_55030"
     CDS             complement(3340..4197)
                     /locus_tag="V0Z23_RS27975"
                     /old_locus_tag="KAM621c_55030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005975789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_326978234.1"
                     /translation="MILEDNIYNIDRYKNQIQRYALCTDNFNDGVYRNPKEKALLKKYI
                     GFNNKSFVNGLVFDIDHEKGALAWDFADLPKPNVIIQNTANGHAHLLYALKNPVLKTDS
                     AKIKPLKLASIVQCGFTERLNADRAYADILMKNPANINEWRTTWTNTEAYDLEYLADFV
                     PETITTEKVKKGGIYGLGRNVNLFEDLRHSAYKEVLKYKANKSEYDFYNDMLSRAVMLN
                     NYCNPTDPLSFNEVKQVCQSISKWTWRNFSTEKFSAIQSYRASKKRSCKKSKDTEDFFK
                     NLGV"
                     /name="replication initiation protein"
ORIGIN
        1 gtgcttctgg tgtattccaa aactttctat taattctaaa cataggatca cgatctaaac
       61 cttctgcaat gattaaatgt gcaacggctg aaattggttc atttttagaa aagcactctt
      121 ccgatttttc taaatgctcg atcctctggt tatccaaagt tttatgagat attttcttgt
      181 tatatggtgt taaatattta ttagcaatat cagcccaatt ttctctatgt ctatttatta
      241 aatctttatc ctgaaagttt cgtaacttct tatcaaactt tccgtttatc atgggtctta
      301 aagtagtcat aacatgagca tgaggattat cactttctaa atcgtgaaat gaaatatcgc
      361 aaacaatatt tttactgaca aaattttgat ggcaatattc tttgacgagg tttatttttt
      421 cttctcgtga caactcaacg ggaattgcta gatcgaaata tttcgatagt tgagcatcct
      481 tccttttttc tatgtcctct aaatggttcc acaaaacggc aggatctaaa tattttttgt
      541 cgccgttttt gggtagtaaa acctcgtgat aatccaaccc tgattttttt gtataatccc
      601 atacatcacc agttctatta tcaacgattt tagaacgggt attatatgcg gataaatagc
      661 aagaactctt gccgtcactt ctttttaaaa tgctaaaaga taaatgatat atagccataa
      721 atttactcct aatttgtcgt tatattttgc cccgcttttt tggtgttggg gttatgtttc
      781 aaatcgcaag atttggaacg gttttagcct cgcagagacc acacgttgcg aagcaatgta
      841 taagtgggca ttctactatg gttgtttttc gcttcgctca aaccaacaca agtagaattg
      901 aagtttttct tttgctgaat agaaccgttc tcctaaatat attttaaaac taatatagaa
      961 attagcaata atatatttga caatataaaa tataaattgt atttaaaaag aataataata
     1021 atagtgaggt tttaatatgg actatttaat ctttgaaata agaaacatga ctaaatacga
     1081 aatgaaactt tttattgaag cattaaaaga agatcaagac aaaaaagagt ttttcgagtt
     1141 attcaaaaag tattatgtga aaacaaatta tagttcaggc aagaatttat ttaaaacaga
     1201 aagaaaagaa cagaggattg caaactataa aattgcaaga cttctaaaca ccattaaaac
     1261 aataaatata gataatataa gagaggcttt ttatgtcgga gaaactaaat aaaaaaacat
     1321 tagatcagaa tataaataaa cttgaattag aattacagaa attaaagagc aaaaaaaaga
     1381 acataagaag aaaagaaatt gaacataaaa aattcgttct cggtggtttg gttgctaaat
     1441 atttttccgt tgatgttgat ttagaattta ttgaagattt attaaagcag gcaagcgaac
     1501 aattgaacgg aacaaataag aggataaatg aagaaaatct aaatagtaat gatattgctt
     1561 ggaaggattt agaatgaaga aaagatataa atttttatac ttcgatttcg taaagaaaaa
     1621 gaatgagaat gaatatgagt ttaaacaatc aaaagaacaa ataaaaaagt ttaaagatga
     1681 ttacttaata aatatcactt ctcatataga tctttgtatt ccgaaggagt cagaagatcg
     1741 ggtttcttat tttgctaaat ttgctcaatt gcttggtgca aatactaaaa gatttagatc
     1801 tgaattatca tatgcattat gtagtgtttt ttttgaaata tatgaatatg ataaaactta
     1861 tcactcagcc caaaatttta tatttgattt aacgggtaaa aaactaacaa gagaaatagc
     1921 ccttaaagag ttttcaaatg tagataattt gtctggaaat ttcagcagat cagaaagtag
     1981 tattttccac aaaaaaaaca agcaaaaagt ggaagaaaac aaaaaagagt ttttgagtgt
     2041 gtttccatat aaatcaatta gttacagtat ggattctgtg ataaatgaag caaaagaatt
     2101 agtgaaaaac aaggcgaaaa agtagagttt ttatgctcgg tttttcatag ataaaagata
     2161 tatcttttaa ctcggcggcg ggtggaacat agcaaacttg tttgctgtgt tacagccgtc
     2221 tattcttttg actttaccga atctgtgcaa tgtgataaaa aatggcttaa aatcgcatac
     2281 aggaggtttt atggctattt caaatattga tcgcatggct cttctaaaag aagaaatgaa
     2341 actgttgaga aaacagcaaa aagaagagaa acaaaagaaa gttgagaatt tgatttttat
     2401 gattggtcaa tctctggttg aacaatcagg cattaatgca aaaaaagaag aagaaatttc
     2461 ttcactgctt tattacatta aaaatattga aaacaagcca gaaggttttg tttttgataa
     2521 aactaaattt gaaaagcacc ttgataaaaa gccaacacct cgaaaaacca caaatacaac
     2581 aacaaaacca caacaaaaaa ctgaatgata ttgattggtg gcactctttc tttgagtgcc
     2641 tttttataga taatagatat atcttttaac tatcaaatag gggctgtaat ggataagcaa
     2701 tcaataatga atttgattga tgatctcaat gtgggaaagg gttttttaat tgctattggt
     2761 gatttgtatt atcaaacaga caatacagaa cttgtgtcat catttttgaa atcaaaaatt
     2821 gatgaggttt acacaaatta ttttcgtgat gtttttgttg atttatccaa tcaagaattt
     2881 aaagatcatg ctttttatgt ggcaatagat aatttgctaa aactcaaaac aaaaaatatt
     2941 gaaaatataa gagagatttt ggcgattgtt tcagccattg atgaaatgat tgaagactac
     3001 aagcaggcat tggatattta aagataatag atatatcttt taactatcaa atagccctgc
     3061 aaagggctat caatgagttt ttaaatctaa tgctaaatat cttctgtaat atgcaattgc
     3121 tgcattctct gttgtgttca ttttctctgc aacttctctc cattttaaac ccgaagatct
     3181 taaatggaaa gcaaggcttc ttttttcctg tgcttcttgc tcataatctt cccttgattg
     3241 ggctatgtat ctttttactg ttctaacatg acagcctttt atttctgcgg cttcttttgc
     3301 tgacatattc ctttttcttt gtatttttgc tgtcattttt taaactccta aattcttaaa
     3361 aaaatcttct gtatcttttg actttttaca gcttcttttt ttgctggctc tgtatgattg
     3421 tatcgctgag aatttttctg ttgagaagtt tctccatgtc cattttgaaa tgctctggca
     3481 aacctgcttt acttcattaa atgataacgg gtctgtcgga ttacaataat tatttaacat
     3541 aacagctctt gaaagcatat cattataaaa atcatactcg cttttattag ctttatattt
     3601 caaaacttct ttatatgctg aatgtctcaa atcttcaaat aaattaacat ttcttcctaa
     3661 accataaata ccaccttttt ttactttttc ggttgttatg gtttcaggaa caaaatcggc
     3721 taaatattct aaatcatagg cttctgtatt agtccaagtt gttcgccatt catttatgtt
     3781 tgctgggttc ttcatgagaa tatcagcata tgccctatct gcattcaatc gttctgtaaa
     3841 accacactga actattgaag ctaattttaa aggctttatc tttgctgaat cagtctttaa
     3901 aacagggttt ttaagtgcat acaataaatg agcatgacca ttagccgtgt tttgaattat
     3961 cacattgggt tttggtagat ctgcaaaatc ccatgctaaa gctccttttt cgtggtctat
     4021 atcgaaaact aaaccattaa caaaagactt attattaaag cctatgtatt tttttaaaag
     4081 tgccttttct tttggatttc tatatacacc gtcattaaag ttatcagtgc ataaagcata
     4141 tctttgaatt tgatttttat atctatctat gttatatata ttgtcttcaa gtatcatttt
     4201 atattccttt ccttatagct cctgttttcg cggtcgccaa actttgaaaa agcaggggct
     4261 ttattttttg tttttatatt tatttccaac cttgccaatt gataacagat cttgaaacct
     4321 tgtcaagtgt gacatttagc catatcagat attagggtgt ttttgggtcg gttttgacct
     4381 tacggaaacc tatgcttgta atccataggt gccctttatc aatggcactc gttcaaatca
     4441 tttgaaaaac tatgattgag ctatgtctgg tgtaactaca caagataagc aaaacagatc
     4501 gccgaaaaat atttttactt caactttggt aatccagtct tcaaatcgac ttgctcagaa
     4561 gcctcctgag cgattttttt gttataggca atatgtttgc ttaacttggt agctaaatcg
     4621 tctctggaag cgattgacgg cgttttagag gcattgttca aaacttcttc ttttcgttga
     4681 tcgagagtct taaaggtaaa tgcctgcttg ttataaagag gcttcttctt attctgtttt
     4741 cttatattgt cgaagatctc aagtaaactt ttcttcatcc agtttttgaa cacttcaaaa
     4801 gtgcctgctt tttttgcaat gttctttata tcaacaaagt tacttgcaaa tgcttttgcc
     4861 tcgtcaaatt ttt
//
