LOCUS       NZ_AP026381             7393 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Aeromonas caviae strain KAM497 plasmid pKAM497_6, complete sequence.
ACCESSION   NZ_AP026381
VERSION     NZ_AP026381.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446293
            Assembly: GCF_024347715.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 03:51:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,577
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,269
            CDSs (with protein)               :: 4,269
            Genes (RNA)                       :: 159
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 123
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 51 of 149
            Pseudo Genes (incomplete)         :: 105 of 149
            Pseudo Genes (internal stop)      :: 15 of 149
            Pseudo Genes (multiple problems)  :: 20 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026381.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM497"
                     /db_xref="taxon:648"
                     /plasmid="pKAM497_6"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            1..708
                     /locus_tag="OCZ21_RS22860"
                     /old_locus_tag="KAM497c_44290"
     CDS             1..708
                     /locus_tag="OCZ21_RS22860"
                     /old_locus_tag="KAM497c_44290"
                     /inference="COORDINATES: protein motif:HMM:NF015076.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_261950824.1"
                     /translation="MVKQAIQADEFVERLPLRPYCTDDPAQGLHIRPQATALAFRHIQH
                     NPPPHVSCIVFDVDRKPYEQRREGYQEWRERGLPAPHWIAINPENGNYHLGYLLAAPVA
                     RTNAARLKPLRYLAAIEHVLAKKLGADMGYVGLITKNPVHSDWWTIWHNHVPYSLDYLA
                     EFCPDADLAAYNRRSGKEASGLGRNVTVFDNVREWGYCAVREYWRPNGYEAWAEAVRAA
                     CESANAFGREQGG"
                     /name="replication initiation protein"
     gene            701..1000
                     /locus_tag="OCZ21_RS22865"
                     /old_locus_tag="KAM497c_44300"
     CDS             701..1000
                     /locus_tag="OCZ21_RS22865"
                     /old_locus_tag="KAM497c_44300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_261950825.1"
                     /translation="MASEIKATAKSIARWIWRHLTPTSFADYVDRTHTTEIQARRGAKG
                     GKVSKGGGRPSKAADLLPAVLELKAQGYSNRDIAEDLGIGSASVSRYLSKISPE"
                     /name="LuxR C-terminal-related transcriptional regulator"
     gene            complement(1152..1442)
                     /locus_tag="OCZ21_RS22870"
                     /old_locus_tag="KAM497c_44310"
     CDS             complement(1152..1442)
                     /locus_tag="OCZ21_RS22870"
                     /old_locus_tag="KAM497c_44310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128318465.1"
                     /translation="MKDNADKGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRAKQQENN
                     VTVTINRGLLEGLDAQMQAIRDGGSAVVLTSEQAGEILRALRTAENKQLRG"
                     /name="hypothetical protein"
     gene            1640..1978
                     /locus_tag="OCZ21_RS22875"
                     /old_locus_tag="KAM497c_44320"
     CDS             1640..1978
                     /locus_tag="OCZ21_RS22875"
                     /old_locus_tag="KAM497c_44320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_128318466.1"
                     /translation="MPTEQPKRLKTIKVRVTETEWASLRAACPKAQLAEWMREHCLGVV
                     PQQRRTPPPTIDPALLRQVAGIGNNLNQIARRINASPLTTLDKVEYLAALAAVARELEG
                     LRSDRQNP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2178..>2612
                     /locus_tag="OCZ21_RS22880"
                     /pseudo=""
     CDS             2178..>2612
                     /locus_tag="OCZ21_RS22880"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2665..3084
                     /locus_tag="OCZ21_RS22885"
                     /old_locus_tag="KAM497c_44330"
     CDS             2665..3084
                     /locus_tag="OCZ21_RS22885"
                     /old_locus_tag="KAM497c_44330"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_234932339.1"
                     /translation="MAKTFEQTSKQQADDIESSLKPEFEKHEKAIKKALDSSRQSISDA
                     IQDQQKRIGWLLLKSWGVMLISALLLLVATSGTLWYQGNLIAERQATLEELAKKGGKLI
                     LSDRCKDENGKHRFCIRMNVDEGKFGDDYMIPKGY"
                     /name="MbeB family mobilization protein"
     gene            3088..3321
                     /locus_tag="OCZ21_RS22890"
                     /old_locus_tag="KAM497c_44340"
     CDS             3088..3321
                     /locus_tag="OCZ21_RS22890"
                     /old_locus_tag="KAM497c_44340"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_113740815.1"
                     /translation="MTELERHLLNALETLQSEQEQQWRSLQTACDSLLGLYETTKKENA
                     SLQGEVRALRQQCEHLSKQVSSLATQVASLKA"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            3650..5548
                     /locus_tag="OCZ21_RS22895"
                     /old_locus_tag="KAM497c_44360"
     CDS             3650..5548
                     /locus_tag="OCZ21_RS22895"
                     /old_locus_tag="KAM497c_44360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="WP_223956766.1"
                     /translation="MHKRKFTTLAFYSLALVLLLGLGLGLHQYYIYKVTREAHKTTVDR
                     NLSLMHMRADGYLERLALDGEFIASTPILRMYLENPSAETLAGAQDALRTLASLRPYLD
                     QLRYIDQQGMERIRVNQRGGPPQLATELQNKSDRDYVQRGLALTAGGVYFSPIDLNIEH
                     GQIERPFHPILRSVAKVMVNGSPAGMVVQNANVDELEKRLRTELPTAVAQPVVLNSSGG
                     WIMGGGEKDWLFSAKPDAPDALLSTQEPALWEKIQASSSGQFEYRGECHYYAWYQFKQQ
                     QWQAERWLVAQRSAGQACGHLTASAVKTWATQLVVMSTFTVPLLVLWLLSRWNARELRR
                     GLRETNSQLELITSEADVALLMVDHECRVCWINPESERLLGWKTDELVGANLHERTHIR
                     NGEDLHSGPCPTLQALQTGQRYRNDRDRVLAKSGEILNVSMRVSPFGEGEARNAIVTIA
                     DVGDAVDREQRLTRLATTDPLTGALNRRSIMERLQDMVEHQKMPPCVLMGDIDFFKKVN
                     DTYGHAAGDEVLKTFTNTIRQLLRKGDFLGRLGGEEFVVALENTELPNAQTMAERLRLA
                     IANSQTPNDGGIPIAITASFGLALYSRDESVDAWLARADAALYRAKQSGRNRVEIA"
                     /name="diguanylate cyclase"
     gene            complement(6008..6436)
                     /locus_tag="OCZ21_RS22900"
                     /old_locus_tag="KAM497c_44370"
     CDS             complement(6008..6436)
                     /locus_tag="OCZ21_RS22900"
                     /old_locus_tag="KAM497c_44370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_128318468.1"
                     /translation="MHNTKPRTVLSLKKAPPQPEITQAAQQSTAIPTKRKIPAAFPCLQ
                     SHWPELFNLKHPTPLKIGILAPVIASLKQQDEPYTDTQIGKAIGWYCRRFEYLKALTNA
                     PHRMGIDGNTFPISDQDRQNAMKQIKARIKEARSCPKR"
                     /name="ProQ/FINO family protein"
     gene            6577..6828
                     /locus_tag="OCZ21_RS22905"
                     /old_locus_tag="KAM497c_44380"
     CDS             6577..6828
                     /locus_tag="OCZ21_RS22905"
                     /old_locus_tag="KAM497c_44380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="WP_128318469.1"
                     /translation="MPTVLIIGGWRVVIYPNDHRPAHVHVIGGGCEAVFNLSGDQGGPE
                     LRENYGFDSKALKRIWAELQGNQAHLISQWEVIHGTNR"
                     /name="DUF4160 domain-containing protein"
     gene            6812..7234
                     /locus_tag="OCZ21_RS22910"
                     /old_locus_tag="KAM497c_44390"
     CDS             6812..7234
                     /locus_tag="OCZ21_RS22910"
                     /old_locus_tag="KAM497c_44390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="WP_128318470.1"
                     /translation="MELTDEMIAQANKRGSEARKRSPVATSVRYDRRIGRVVIALSSGL
                     ELAFAPHLAQGLEAAKPSDLQEIEISPSGLGVHFPRLDADLYLPSLLEGLLGSENWAAS
                     KMGAKGGKAKTPAKVAAARANGAKGGRPRKNVPAAA"
                     /name="DUF2442 domain-containing protein"
     Typing          2..943
                     /name="rep_cluster_1010"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.034"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          2500..3515
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.64"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atggtaaaac aagcgattca agccgacgaa ttcgtcgaac ggctccccct ccgcccctac
       61 tgcaccgacg atccggcgca gggtctgcat atccgcccgc aggcaactgc gctggccttc
      121 cgtcacattc agcacaaccc accgccgcat gtctcctgca ttgtgtttga tgtggacaga
      181 aagccctatg agcagcggcg agaagggtat caagagtggc gagagcgtgg cttgcctgcg
      241 ccgcattgga tcgcgatcaa ccccgaaaat ggcaattacc accttggcta tctgctggcc
      301 gcgccagtgg cgcgtaccaa tgcagccagg ttaaagccac tgcgctatct agcggctata
      361 gagcacgtgc tggcgaagaa gctgggcgct gacatggggt atgttgggtt gatcaccaaa
      421 aaccccgtac acagcgattg gtggacgata tggcataacc atgtgcccta ttccctcgac
      481 tatctcgcgg agttctgccc agatgctgat ttagcggcct acaaccgccg tagcggtaaa
      541 gaggcgagcg ggctggggcg taacgtcaca gtgttcgata acgtgcggga atggggctac
      601 tgcgcggtgc gggagtattg gcggcccaat ggctatgaag catgggctga ggctgtccgt
      661 gccgcttgtg agagcgctaa tgcgtttgga cgcgagcaag gtggctagtg agatcaaggc
      721 cactgccaag agcatcgccc gttggatctg gcggcacctt accccgacct cgtttgcgga
      781 ttacgttgat cggacacata ccaccgagat ccaagcgcgg agaggggcta agggcggcaa
      841 ggtatccaag ggcgggggga gaccgagcaa ggccgctgat cttctgcctg cggtgttaga
      901 actcaaggcg cagggctaca gtaaccgcga cattgccgag gatttaggga tcggttcggc
      961 aagtgttagc cgctatctct ccaaaatctc gccagagtga tccaaaacaa gcctatatca
     1021 gataacagcg cccgaagggc gcttcttgtc tctagagaat cattcaagag agaggatctt
     1081 ggcgaggggg gtaacggcga tcccgtaggg ctgtccatca ggccatgtgt tcggcctgtg
     1141 ggcagtagct gttatccccg gagctgtttg ttctctgctg ttcgtagcgc ccgcaagatc
     1201 tccccggcct gttccgaggt cagtaccacc gctgaacccc cgtctctgat cgcctgcatc
     1261 tgggcatcca gtccttccaa tagccctcta tttatcgtca cagtaacgtt attttcctgc
     1321 tgttttgccc ggtaggcagc ttgtcgttct gcccctgttt tggctgatcc ggttttgggt
     1381 ctaccccgtc cgcgcttgat gccaaacatg tctccggttc ccttatctgc gttgtctttc
     1441 atcgtccaaa ttcccgtcac agtgaccata aatattattt gtcactgtga cacaaaaagc
     1501 attcatccgc accctcaaaa ttcaacccat gttaaagtca taagcaagga cacacagggg
     1561 gcccgcttcg cggtctatcc cctgtggcct tgttcagggg gcaagccccc cgaaaccccc
     1621 gaaaaaacgg aggcaactga tgccgacaga gcagccaaaa cggctcaaga ctatcaaggt
     1681 cagagtcacc gaaaccgagt gggccagctt gcgggcggcc tgccccaaag cccagttggc
     1741 ggagtggatg cgcgagcact gcttgggggt tgttccccag cagcggcgca ccccaccgcc
     1801 aaccatcgat ccggcattgc tccggcaggt ggccgggatc ggtaacaacc tcaaccagat
     1861 cgcccggcgc atcaatgcca gcccgctgac cacattggac aaggtggaat accttgctgc
     1921 gttagccgcc gtggcccgag aactggaggg gctacgcagt gatcgtcaaa atccatagcc
     1981 ggggagtcgg tcgcggatct ggtccggtgg aatacctgct tggcaaagac gggaaccggg
     2041 acggggccac gctggatcgc ggcgaccccg agatggtcaa ggagttgata gacggcagcc
     2101 attacgccca gcggtacacc agcggcgtgt tgtcgttcca cgaaccggac ttgccgaggg
     2161 agaccaaaga gcggttgatg gacgagttcg agcaagccct gttgcccgga ctggacgccg
     2221 atcagtatgc ggtgctgtgg gtcgagcacc gggacaaaga ccgcctagag ctgaatttcg
     2281 tggtgccgaa cgtcgaactg ttgtccggca aacgccttca gccgtatttc cacggggcgg
     2341 acggccaccg gatcaacgca tggagaacgg ccatcaatgg cgagttggga ttgcacgacc
     2401 cggacgaccc gatcaatcgg caggccgtaa ccacggccaa agatctcccc cggaacaagc
     2461 tggaagccga acaagccatt acaggcggct tgttgggcat ggctgcggcg gggctcgtca
     2521 aaaaccggga ggacgttctc aagaccctga ccggggcggg gttcgacgtt accaggacaa
     2581 ccaagaacag catcagcatt gccgaccccg aagggggccg gaatatccgc ctaaaggggg
     2641 cgatctatga gcaagatttt agacatggca aagaccttcg agcagacatc gaagcagcaa
     2701 gccgacgata tagagagcag tctgaagccc gagttcgaga agcacgagaa agctatcaaa
     2761 aaggcgttgg actcaagcag gcagagcatc agcgacgcca tccaagacca gcagaagcgg
     2821 atcggctggc tcctgctcaa gagctgggga gtgatgctga tctcggcctt actgttgctg
     2881 gtcgcgacga gcgggacgct gtggtatcag gggaacctga tcgccgagcg gcaggccacc
     2941 ctagaggagt tagccaagaa gggggggaaa ttgatcctca gcgacaggtg caaggacgag
     3001 aacgggaagc accggttttg catcaggatg aacgtggacg aagggaagtt cggggacgac
     3061 tacatgatac caaagggcta ctaaacgatg accgagctag aacggcactt attgaacgcg
     3121 ctagagacct tgcagagcga gcaagagcag caatggcgca gcttgcaaac agcgtgcgac
     3181 agcttgctgg ggctgtacga gacgacgaaa aaagaaaacg cgagtttgca gggagaagtg
     3241 agagccttgc gccagcagtg cgagcacttg agcaagcagg tcagcagctt ggcaacgcag
     3301 gtcgccagct tgaaggcgtg atcgcgcagc agacggcgca gacgcaggaa ggtttgttgg
     3361 acgaatacca gcaggcccac gcagagcgga acctgtattt caatcggaag catgagcaga
     3421 aagagatctc cggcttgccg atagacaagg ccagagagta cctacaggaa ggactggagg
     3481 ggcggataga gcggcagcag gcccgcgatt cgggattgga aggccgcgct gaaccgattt
     3541 accattgagt tcggagaccg tatctgcgtc aattgaagtc cgaaccgttt acacaaaaat
     3601 tcggacacgc cctgtgtgag tcctcaaaat ctccggaaag acaacgttca tgcacaaacg
     3661 aaaatttacc actctcgcct tctattcgtt agcgttggtg cttttgctgg gactgggtct
     3721 cggcttgcac cagtactaca tctacaaggt cactcgtgag gcgcataaaa ccacggttga
     3781 tcgcaatctg tcgttgatgc atatgcgggc tgacggctat ctggaacggt tggcactgga
     3841 tggagaattc attgcgagca cccccatcct gcgcatgtat cttgaaaacc cctcggcaga
     3901 aacactggcg ggcgcgcagg acgctctccg gactcttgct tcgctgcgtc cctatctgga
     3961 tcaactgcgc tacatcgatc agcagggcat ggagcggatt cgggtgaatc aacgaggtgg
     4021 gcctcctcaa ttggccacag agctacaaaa caaatctgac cgtgattacg ttcagagagg
     4081 cttggcactc acggctggcg gcgtctattt ctcgccaatt gatctgaaca ttgaacacgg
     4141 acagatcgag cgccccttcc accccatact gcgttcggtg gccaaggtca tggtcaacgg
     4201 cagccctgcg ggcatggtgg tgcagaatgc caatgtggat gaactggaaa aacggttgcg
     4261 cacggaactg ccaacggcgg tggcgcaacc ggtggtgctc aacagcagcg gcggctggat
     4321 catgggcggt ggtgagaagg actggctgtt ttcggcaaag cctgatgccc cggacgctct
     4381 gctatccaca caagagcccg cgctgtggga aaaaatccag gccagttcgt caggtcaatt
     4441 tgagtatcgt ggggaatgcc actattacgc ttggtaccaa ttcaagcagc agcaatggca
     4501 ggcagagcgc tggctggtag ctcagcgctc tgccggtcaa gcctgcggac acttgaccgc
     4561 ttcggcagtt aagacgtggg ccacgcaact ggtggtaatg tcgactttca ccgttccgct
     4621 gttggtgttg tggctgttgt cccgctggaa cgcgcgcgaa ctacggcgtg gacttagaga
     4681 gacgaactcc cagctagaac tcatcactag cgaagctgac gttgccctgt tgatggtcga
     4741 ccatgagtgc cgcgtctgct ggattaaccc tgaatccgaa cgcctattgg gctggaagac
     4801 cgacgagttg gttggtgcaa acttgcatga aagaacccac atacggaacg gggaggatct
     4861 gcactccggg ccctgtccga cgctgcaggc gcttcaaaca ggacagcgtt accgcaatga
     4921 tcgtgaccga gtgctcgcga agtctggcga aattttgaat gtaagcatgc gtgtgagtcc
     4981 gtttggtgag ggtgaagcgc gcaatgccat cgtgacgatt gccgacgtag gggacgcagt
     5041 ggaccgagag cagcgtttga cacggcttgc cacgactgat ccattgaccg gcgcactcaa
     5101 ccgtcgatcc atcatggagc gtctgcaaga catggtcgaa caccaaaaga tgccaccttg
     5161 cgtgttgatg ggcgatatcg attttttcaa gaaagtcaac gacacctatg gacacgctgc
     5221 gggcgatgag gtactcaaaa ctttcaccaa cacgatacgt cagttgctgc gcaagggtga
     5281 ctttttaggt cggctcggcg gtgaagagtt cgtggtggcc ttggagaaca ccgagctgcc
     5341 aaacgcccaa accatggccg aacgcctgcg cctagccatt gcaaatagcc aaaccccaaa
     5401 cgatggcggt attcccatcg caatcaccgc cagctttggc ttggcgctgt acagccgtga
     5461 cgaatcggtg gatgcgtggc tggcccgcgc agatgctgca ctgtaccgcg ccaaacaatc
     5521 ggggcgaaac cgggtggaaa tagcttagcg ctgctggggt gctgtttcgc aggatgccct
     5581 gcagacgcag atcacgccca ttcggccgga aatcctcgcg cctatcgcat ccgacggaaa
     5641 tctacctgct cgcattcatt gcgattgcgt ttccacacag cctcggtcgg gtgccgtcgc
     5701 gtgcgcagtg tcaaagctga catgacgaat acgtgagaag ggctgatgct cgccgtccgg
     5761 tcaagctgtt gagtcgcccc gggttctacc ctgttttcac ggacacatct aaaagaggca
     5821 taatgtgcct gtaaggagtg ttcatgacca agcgaagaaa attcagtcca gagttcaagc
     5881 gagaagctgt caaactgacc cagcagccgg gaacgggggc ggggctttga gatgtagctg
     5941 ccgcgctgcg cttgccgact cccaaacgaa gcgcgacaga ctgcgctagt cgctcttgtt
     6001 tgggagttca tcgtttgggg caggatctag cctctttgat gcgcgccttg atttgtttca
     6061 tcgcgttttg gcggtcttgg tcgctgatgg ggaatgtgtt gccatcaatc cccattcgat
     6121 gaggagcatt ggtgagggct ttgaggtact caaatctacg gcaataccag ccaatggcct
     6181 tcccaatctg agtatcggtg taaggctcgt cctgttgctt caggctggcg atgacaggag
     6241 ctaggattcc gattttaagt ggagtgggat gtttgaggtt gaatagctcg ggccagtgcg
     6301 attgtaggca agggaaagcc gctgggattt ttctcttcgt aggtatcgcg gtggactgtt
     6361 gtgctgcttg ggttatctca ggctgtggtg gggccttttt aagactcagt actgtacggg
     6421 gcttggtgtt gtgcatggac gtatacctgc ggaaatgatg tttgcaggca agttatcgaa
     6481 acccccatca ggatgcaaaa acagttgaac tatcgataat gcacccattc gagtgtatta
     6541 ttgcgtttat agaaaccaaa acagttaggt tgtttgatgc caacggtgtt gatcattggt
     6601 ggctggcgag tggtgattta tccaaacgac catcgtccgg cgcacgtaca cgtgattggg
     6661 ggcggctgcg aggcggtgtt taatctctcg ggtgatcaag gcgggcctga gctgcgggaa
     6721 aactacggat ttgatagtaa agcgctgaaa cggatctggg cagagttgca aggcaatcaa
     6781 gctcacctga taagccaatg ggaggtgatc catggaacta accgatgaaa tgatcgcaca
     6841 agcaaataaa cgcggttctg aagcgcgtaa gcgtagtcct gttgctacga gcgtgcgtta
     6901 tgaccggagg attggacggg tcgttatcgc cttgtcttct ggtttggaac tggcctttgc
     6961 ccctcatctg gcacaggggt tggaggccgc gaagcctagc gacttacagg agatcgagat
     7021 ctctccgtca gggttgggag tgcactttcc gaggttggat gcagatctct acttaccttc
     7081 cctgctggaa gggttgttgg gctctgagaa ctgggcggca tccaagatgg gggcgaaggg
     7141 cggcaaggca aaaacccccg ctaaagtcgc agcggcgcgg gccaacggcg ctaaaggtgg
     7201 gcgtccaaga aaaaacgtgc ctgcggcggc ctgagtgcgg taacgtactg cctagaaaag
     7261 acaaaccccg aatctctgtt catcgacttg gcggaagagc acagagagtt cggggccgtt
     7321 cacgaagaac agagaaagca tatcacatga ctgacctcac acaagactgc accgccaata
     7381 agcgcggtga cgg
//
