LOCUS       NZ_AP026380             8528 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Aeromonas caviae strain KAM497 plasmid pKAM497_5, complete sequence.
ACCESSION   NZ_AP026380
VERSION     NZ_AP026380.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446293
            Assembly: GCF_024347715.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 03:51:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,577
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,269
            CDSs (with protein)               :: 4,269
            Genes (RNA)                       :: 159
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 123
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 51 of 149
            Pseudo Genes (incomplete)         :: 105 of 149
            Pseudo Genes (internal stop)      :: 15 of 149
            Pseudo Genes (multiple problems)  :: 20 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026380.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM497"
                     /db_xref="taxon:648"
                     /plasmid="pKAM497_5"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            join(7100..8528,1..134)
                     /locus_tag="OCZ21_RS22795"
     CDS             join(7100..8528,1..134)
                     /locus_tag="OCZ21_RS22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_261950822.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFTE
                     EELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPIAK
                     NGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSA
                     MAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGR
                     TDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQ
                     IATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQAAQ
                     VIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAA
                     RTQQSTTDIQSMISALQERAQSAVTVMEQSSRQADTSVAHAEEAATALDGIGQLVNEIT
                     DMNAQIATAVEQQGAVSEVINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELAKQFWE
                     KRG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(429..629)
                     /locus_tag="OCZ21_RS22800"
                     /old_locus_tag="KAM497c_44170"
     CDS             complement(429..629)
                     /locus_tag="OCZ21_RS22800"
                     /old_locus_tag="KAM497c_44170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128274297.1"
                     /translation="MSDRFPGLMLWHTVAVMAAVFAAKALLYWAADWLGLGPLYRALAG
                     PSWDVAAWIAALALLLRWMRV"
                     /name="hypothetical protein"
     gene            complement(637..771)
                     /locus_tag="OCZ21_RS22805"
                     /old_locus_tag="KAM497c_44180"
     CDS             complement(637..771)
                     /locus_tag="OCZ21_RS22805"
                     /old_locus_tag="KAM497c_44180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_261950823.1"
                     /translation="MNDDDGSRWIVIDTEYLRQHVAHYALLSADTWRQRLLDAGVEVD"
                     /name="hypothetical protein"
     gene            complement(1025..1366)
                     /locus_tag="OCZ21_RS22810"
                     /old_locus_tag="KAM497c_44190"
     CDS             complement(1025..1366)
                     /locus_tag="OCZ21_RS22810"
                     /old_locus_tag="KAM497c_44190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011116720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_202979152.1"
                     /translation="MTELERLLTAQFERMEQRHQDETKALRAQLDAQQQSLEGLQTQLQ
                     STLGEYETHCASLHTAFVALEKSTRRELASMGRSLDQTNRATGAALGSLNSSVSIVKSS
                     LESLNKAQS"
                     /name="mobilization protein"
     gene            complement(1367..2068)
                     /locus_tag="OCZ21_RS22815"
                     /old_locus_tag="KAM497c_44200"
     CDS             complement(1367..2068)
                     /locus_tag="OCZ21_RS22815"
                     /old_locus_tag="KAM497c_44200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202979151.1"
                     /translation="MEKGGELKTRLSERLSQRQAEELAEVATLTEANLTQLSERLTKHV
                     SAELSTTRDAIRQQSEQITTLLGSQQSALEQQQATVDALKRLSLKDLEQIQKGLNDAIT
                     LAQGKAIEQAIDPLRTALEQLKMDGEALSRVTFRGWLKPLLISMAVLLGAGGSTWGVAA
                     YLAKEITGQLTELQNLNQQIGTAQRTLDSLPQGVTFVRDRDGRYIVARQIDTPFKMDEG
                     TYKGLMAVGVK"
                     /name="hypothetical protein"
     gene            complement(<2094..2783)
                     /locus_tag="OCZ21_RS22820"
                     /pseudo=""
     CDS             complement(<2094..2783)
                     /locus_tag="OCZ21_RS22820"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2773..3174)
                     /gene="mobC"
                     /locus_tag="OCZ21_RS22825"
                     /old_locus_tag="KAM497c_44210"
     CDS             complement(2773..3174)
                     /gene="mobC"
                     /locus_tag="OCZ21_RS22825"
                     /old_locus_tag="KAM497c_44210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_128274291.1"
                     /translation="MTKPELKTTTASLRLDPATLAKWRKGACDEGLTLSDWLRAKVDAD
                     QQTHIAPARSRRERDPRHLADPALMRQLAALGSNLNQIARAVNECRLVGSPVYLVELLA
                     LLRSIEEAAGTLLPQLPPPPNREGDGHDD"
                     /name="mobC"
     gene            3387..3677
                     /locus_tag="OCZ21_RS22830"
                     /old_locus_tag="KAM497c_44220"
     CDS             3387..3677
                     /locus_tag="OCZ21_RS22830"
                     /old_locus_tag="KAM497c_44220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128274289.1"
                     /translation="MKDTKDQGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRAKQQDNN
                     VTVTINRALVEGLDAQMQVIRDGGSAVVLTPEQAGEILRALRTAENKQLRG"
                     /name="hypothetical protein"
     gene            complement(3827..4771)
                     /locus_tag="OCZ21_RS22835"
                     /old_locus_tag="KAM497c_44230"
     CDS             complement(3827..4771)
                     /locus_tag="OCZ21_RS22835"
                     /old_locus_tag="KAM497c_44230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_042650711.1"
                     /translation="MNRAQLDLFRERLPRKPYHTDEFTTGLSIADVSRALGARYIQPNG
                     PTHRHWLVFDVDHAAATLSWDDVGAPAPNITVTNRANGHAHLIYGLETPIRTAPDGKVA
                     PLRYAAAIEAALREKLGADLGYSGLICKNPLHTHWLVQVWEPASYDLSWLADYLDLSPY
                     NGRKQLPAYGLGRNCTLFEKTRQWAYKAIRQGWPGYEAWLSAVIDRAIGYNVQFEPPLP
                     ANEVRHIAKSIAKWTHRNLTPAGFSAVQAARGAKGGKVSKGGGRPSNSGKARADLLPEV
                     LRLKGMGYSNRDIGEDLGISSSTVSLYLKRESE"
                     /name="replication initiation protein"
     gene            complement(4981..5265)
                     /locus_tag="OCZ21_RS22840"
                     /old_locus_tag="KAM497c_44250"
     CDS             complement(4981..5265)
                     /locus_tag="OCZ21_RS22840"
                     /old_locus_tag="KAM497c_44250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017411299.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_042650712.1"
                     /translation="MQVKWLRKAALNLEDAHDHLAKENSRVAQEFVLEVYRLVHLLVDQ
                     PAMGRPGRVPGTRELVLQPYPFLIPYRVKGEEIQILRVFHTRLRLPSHW"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5255..5494)
                     /locus_tag="OCZ21_RS22845"
                     /old_locus_tag="KAM497c_44260"
     CDS             complement(5255..5494)
                     /locus_tag="OCZ21_RS22845"
                     /old_locus_tag="KAM497c_44260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005740683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_016946643.1"
                     /translation="MSVMSVRLPDEVEQQLGQLAQSTGRTKSWLANQAIQDYLAREAWQ
                     ITEVEAALREADAGDFVPEKEMMAKFNRWGINAG"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5735..5875)
                     /locus_tag="OCZ21_RS22850"
     CDS             complement(5735..5875)
                     /locus_tag="OCZ21_RS22850"
                     /inference="COORDINATES: protein motif:HMM:NF013752.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_002431002.1"
                     /translation="MIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERS
                     H"
                     /name="IS5 family transposase"
     gene            complement(5814..6716)
                     /locus_tag="OCZ21_RS22855"
                     /old_locus_tag="KAM497c_44270"
     CDS             complement(5814..6716)
                     /locus_tag="OCZ21_RS22855"
                     /old_locus_tag="KAM497c_44270"
                     /inference="COORDINATES: protein motif:HMM:NF033581.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_261950821.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGSGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLADRRAPQGKNLETASTQEQNGHQHRIHESQHPGQGGAPISH
                     HQATVRLRESQIQGVAEKR"
                     /name="IS5 family transposase"
     Typing          complement(3828..4764)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.659"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttatcaatat acgcgatgct gctgatacca atgtacagac cgggcagaat aatttgcaaa
       61 gtgcgaaatc tgtcgctcag ttaactagcg ctctgagcga actggcaaaa cagttttggg
      121 aaaaacgagg ataacgcttt tcagtatctc gacagggata acttacttta ccatcggtat
      181 cccttttagc cgcacaaatt ttagccatgg cttttcagga agtcagggct atcagaatgg
      241 ccttagaaag cctagtcaaa gagctgtcac gagaacaccg ttagcttagc gtacgatttt
      301 ttccgaattc tgcggttccc ccggatcctg cagggtgttt ctggatcagg ccacactccc
      361 gaacggggac gactagcgca gtctgtcgtg cccccttcgg gagtcgccaa gcgcagcgcg
      421 gcaggcagtt agacccgcat ccagcgcagc agcagggcca gcgcagcaat ccatgccgcc
      481 acgtcccagg aaggccctgc caaggcgcgg tagagcggac caaggcccaa ccagtccgca
      541 gcccagtaca gcagggcttt cgccgcaaac acggcggcca ttaccgccac cgtatgccac
      601 aacatcagcc ccggaaaccg atcactcaat cgcacctcaa tccacctcca cgcctgcatc
      661 gagcaaccgt tgccgccatg tatcggcgga caacagggca tagtgggcca catgctggcg
      721 caggtattca gtatcaatga cgatccagcg gctgccgtcg tcatcgttca tccagtcgat
      781 ggtcgcatcg aggaggtcga tcagcggttc actatccgcc catagccatg gggagccttg
      841 atagctcgcc agttcatact caaccggggg ggaactggcg aatatgcgcc accgtgtcag
      901 gagtacccaa ccgacctgat ttcatacccg atctccttaa ttattgtcac tgtgacatta
      961 tattacatgc tcaacccgct actctggctc tgctcttttc gcagcaccgc ggccctatcg
     1021 acgatcaact ctgcgccttg ttcaagctct ccagcgagct tttgactatg ctcacactgc
     1081 tgttcaatga cccaagtgct gctccggtag cccggttggt ctggtccaaa ctccggccca
     1141 tgctggctag ctcgcgcctg gtgctcttct ccaaggccac gaaggcggtg tgtaagctgg
     1201 cgcagtgcgt ctcgtactcg cccaatgtgc tctgcaactg cgtctgtaag ccctccaaag
     1261 attgctgttg cgcgtcgagc tgcgcccgca aggctttcgt ctcgtcctgg tgccgttgtt
     1321 ccatccgctc gaattgagct gtcagcagcc gctctaactc ggtcatctac ttcaccccca
     1381 cggccatcag gcccttgtac gtcccttcgt ccatcttgaa cggtgtatcg atctgcctcg
     1441 ccacgatgta ccgcccatct cggtcgcgca cgaacgtcac cccttggggc aagctgtcca
     1501 gggtccgttg ggcagtgccg atctgctggt ttaggttctg aagctcggtc agttgcccgg
     1561 tgatctcctt cgccaggtac gctgccaccc cccaggtact gcccccggct cccaggagca
     1621 ccgccatcga tataagcagc ggtttgagcc atccccggaa cgtcacgcga ctcagggcct
     1681 ccccgtccat cttgagttgc tctagggccg tcctgagcgg atcgatcgcc tgctcgattg
     1741 ccttcccctg ggcgagggtg atcgcgtcgt ttaacccctt ctggatctgc tccaagtcct
     1801 tcaagctcaa gcgtttgagc gcgtccacgg tcgcctgttg ttgttccaag gcgctctgct
     1861 gtgaacctaa caacgtggtg atctgctcgc tctgctgccg aatagcgtcc ctggttgtac
     1921 tcagctcggc gctcacatgc ttcgtcaaac gctcgctgag ctgtgtcaga ttggcctcgg
     1981 tcaacgtggc cacctcggcc agctcctcgg cttgccgctg cgatagtctc tcgctcaagc
     2041 gtgttttcag ctcgccaccc ttctccatac agctctcctt tgagccggat gccctgcggg
     2101 tatccttccg gcctgatcgt gatgtagtcc ttgccctggc gggtgatctc gaattcgagc
     2161 acctcgccca ggtactcgag taattcgccc cggtcgcgga tcagcccctg agccaccgcc
     2221 ccaagcaggt gaccccctat ctgccgcttg atggcagtcc tgtccttctg gcccttgtag
     2281 gcgtcaaagg agggctgtat tagccgtgcc cggagcgggt cgtctggtct ggcccaaccc
     2341 ttgccgtagt tccatttatc tcgcagggca tcaaagtcct ttcgatgtcc gggcggggca
     2401 atgttcagag atttgcccgt gcccaggtga acacggggta ccaagatgtg gacgtggaca
     2461 ccgcctcccg cttcccggtg ctgcacggcg gtcaggtgta cgtcctgcct atccagtccg
     2521 gcaaaggaca gcgcctcgaa gtcgtccagc accgcgccga tctctgcgtc actgggggcc
     2581 tcttcctccg accaggagat caccgcactc cggtatcgat gctgaaaatc gagcgcgtcc
     2641 gccacggcgg cgaactgctg tggatcaccc cgcagcaccg ccaccccggc ccgttcctcg
     2701 cccttgtggt cgtgactgtc cagcaaatag gtcgccgcac tactgccctt gccggtgcca
     2761 tgagccagga acttaatcat catgcccgtc accctcccgg ttcggcggcg gcggaagctg
     2821 cggcagcaac gttccggctg cctcctcgat gctgcgtagc aacgccagca gctccaccag
     2881 gtaaaccggg ctccccacca ggcggcattc gttcaccgcc ctggcgatct ggttgaggtt
     2941 cgaccccaac gccgcgagct ggcgcattag cgccggatct gccaggtggc gggggtctcg
     3001 ttcccgccgc gaccgggcgg gggcaatgtg ggtttgctgg tcggcatcca ccttcgctct
     3061 cagccagtcg gagagggtca ggccctcgtc acaggcaccc ttgcgccact tggctagggt
     3121 ggcaggatcg aggcgaaggc tggccgttgt tgtctttagc tctggctttg tcattggctc
     3181 tccaagcagc gcagcggcgc agggtttgaa ggggcggagc ccctcaccag cccccgttta
     3241 cgttgagccg cgaagcgggc gaaaacgtaa tacggggtcg gctggcttaa taccagtaca
     3301 gcaaaaaaac agggggtcgg caacttgcca attatcgtca cagtgacaca taatcaatta
     3361 ccgttacagt gacaggaaat aacaccatga aggacaccaa ggatcagggg actggggaca
     3421 tgtttggcat caagcgcgga cggggtaggc caaaaaccgg atcagccaaa acaggggcag
     3481 aacgacaagc agcctaccgt gcaaagcagc aggacaataa cgttactgtg acgataaatc
     3541 gggcattggt agaggggctg gatgcccaga tgcaggttat ccgggatgga gggtcggcgg
     3601 tggtactgac cccggagcag gccggggaga tcctgcgggc actgcgaacg gcagagaaca
     3661 aacagctccg gggataagac ctactgccca caggccaaac acatggcctg atggacagcc
     3721 cttcgggatc ggccttaccc ccctcgcacc aatcctctct cttgaatgat tttaagggct
     3781 caaagaaccc cctccggggg ctactatctg ataaggcttg tttcgatcac tcagattcgc
     3841 gtttcaggta caggctgacc gtactggacg agatcccaag atcctcacct atgtcgcggt
     3901 tgctgtaccc cataccctta agcctgagca cctccggtaa gaggtctgca cgagccttac
     3961 cgctattact gggcctgccc ccacccttgg atactttccc acccttggcc cctctggcgg
     4021 cctgtacggc gctgaaccct gcgggggtta ggttacggtg cgtccacttc gcaatggact
     4081 tagcgatgtg cctgacctcg ttggccggga gcggcggctc gaactggacg ttgtacccga
     4141 tcgcccgatc tataacggca gagagccaag cctcataccc cggccacccc tgcctaatcg
     4201 ccttgtaagc ccactggcgc gttttctcga acaaggtgca gttacgcccc aacccgtaag
     4261 ctggaagctg tttgcggcca ttgtaggggc ttaaatcgag gtagtcggcc agccagctca
     4321 gatcgtagga ggcgggctcc catacctgga caagccagtg ggtatgcagg gggttcttgc
     4381 agatcagccc ggagtagccc agatccgccc ccagtttctc ccggagggcg gcctcgatgg
     4441 cggcggcata ccgcagtggg gccaccttgc cgtccggggc agtgcggatc ggggtctcca
     4501 gtccgtagat caggtgggcg tggccgttgg ccctgttcgt caccgtgatg ttcggtgccg
     4561 gggcgcctac gtcatcccag gataacgtag cggcagcatg gtctacgtca aacacgagcc
     4621 aatgccgatg cgttggcccg ttcggctgga tgtaacgagc cccaagggca cggctgacat
     4681 ccgcaatgga cagacccgtg gtaaattcgt cggtgtggta gggcttccgg ggcaggcgct
     4741 cccggaacag gtcaagctgt gctcgattca atgaccaccg cgcatattgg cggcgctaaa
     4801 gggttgtctg gtgtgatcca tgtgatatat ttcacctatt gcataagtac ggccccgaac
     4861 tctctgtaat ctcccgccaa gttgatcaac agaagttcgg ggtttgtctt ttctaggcag
     4921 tacgttaccg cacgaccccc tccatacgca aacgcatacc gccctaccta caagcaccct
     4981 tcaccagtgg cttggtagcc gcaagcgggt atgaaacacc ctcagaattt ggatctcctc
     5041 gcccttcact cggtatggaa tgaggaacgg atagggttgc agcacaagct ctcgtgtacc
     5101 aggaacgcgc cctggccgtc ccattgctgg ctgatctacc aacaagtgaa ccaagcgata
     5161 cacctctaat acaaactcct gggccaccct ggagttctct tttgctaagt gatcgtgagc
     5221 atcctcaagg ttcagtgcag ccttacgcag ccatttaacc tgcattgata ccccaccgat
     5281 tgaacttcgc catcatctcc ttttcaggca caaagtcgcc cgcatccgct tcacgcaatg
     5341 cggcttcaac ttccgtgatc tgccaggctt cacgcgccaa ataatcctgg atagcctgat
     5401 tcgccaacca cgatttggtt cttcctgttg attgggccag ttgccccaac tgttgctcta
     5461 cctcatccgg taagcgaacg gacataacac tcatatacac ctcacaaaac cacgatgttt
     5521 acatgatact acaccttgac tgaatgacag gcacggacaa caacccagag tctcgttgtc
     5581 cgacctgggg aaggtgcgaa taagcgggga aattcttctc ggctgactca gtcatttcat
     5641 ttcttcatgt ttgagccgat tttttctccc gtaaatgcct tgaatcagcc tatttagacc
     5701 gtttcttcgc catttaaggc gttattccca gtttttagtg agatctctcc cactgacgta
     5761 tcatttggtc cgcccgaaac aggttggcca gcgtgaataa catcgccagt tggttatcgt
     5821 ttttcagcaa ccccttgtat ctggctttca cgaagccgaa ctgtcgcttg atgatgcgaa
     5881 atgggtgctc caccttggcc cggatgctgg ctttcatgta ttcgatgttg atggccgttt
     5941 tgttcttgcg tggatgctgt ttcaaggttc ttaccttggg gggctcggcg atcagccagc
     6001 cagtccacat ccacctcggc cagctcctcg cgctgtggcg ccccttggta gccggcatcg
     6061 gctgagacaa attgctcctc tccatgcagc agattaccca gctgattgag gtcatgctcg
     6121 ttggccgcgg tggtgactag gctgtgggtc aggccactct tggcatcgac accaatgtgg
     6181 gccttcatgc caaagtgcca ctgattgcct ttcttggtct gatgcatctc cggatcgcgt
     6241 tgctgctctt tgttcttggt cgagctgggt gcctcaatga tggtggcatc gaccaaggtg
     6301 ccttgagtca tcatgacgcc tgcttcggcc agccagcgat tgatggtctt gaacaattgg
     6361 cgggccagtt gatgctgctc cagcaggtgg cggaaattca tgatggtggt gcggtccggc
     6421 aaggcgctat ccagggataa tcgggcaaac agacgcatgg aggcgatttc gtacagagca
     6481 tcttccatcg cgccatcgct caggttgtac caatgctgca tgcagtgaat gcgtagcatg
     6541 gtttccagcg gataaggtcg ccggccacta ccagccttgg ggtaaaacgg ctcgatgact
     6601 tccaccatgt tttgccatgg cagaatctgc tccatgcggg acaagaaaat ctcttttctg
     6661 gtctgacggc gcttactgct gaattcactg tcggcgaagg taagttgatg actcatgatg
     6721 aaccctgttc catggctcca gatgacaaac atgatctcat atcagggact tgttcgcacc
     6781 ttccttaggg tatagcctag attgacatcc gcgatgcaac ccttaacttg cttgcaccta
     6841 tcgtttccat gctaaatagc tgttccgaat agtagatcac tgtactcgta ccgcttctac
     6901 aggtgacatg gagcacacat cgtcgctgag ccttgtcact tcaggcgttg ccgtcagatt
     6961 gcacaaattc cttacgccac aaggcttccg gcaagatgta gcactattag gatcagttat
     7021 ttagctttat cgtaacgctc aagaaattgg gcttaatgcc gaaaacaata aaataaaaca
     7081 gccaaccctt gaggttctta tgcgccagaa tttaccagtg acgggtcgaa acttagaact
     7141 cccaaaagat gccaatattc tttcgactac ctcccctcaa agccatatca cgtacgttaa
     7201 tcctgacttc attaaaatca gtggtttcac tgaggaagaa ctattaggcc agcctcacaa
     7261 catcgtaaga cacccagata tgccgcctgc tgcatttgag catatgtgga gtacattaaa
     7321 atctggccgc tcatggatgg ggctagtaaa aaatcgctgt aaaaatggcg accactattg
     7381 ggtaagtgct tatgtaacgc caatagctaa gaatggttcg attgttgaat accagtctgt
     7441 aaggaccaag cctgaacctg agcaggtttt ggctgcggaa aaattatatg ctcaattgag
     7501 aagcgggaag gccgcgaggc cgaaattggc tgctagcttt tccgtgaaaa tactcttgct
     7561 catatggggt agtattatat caagcgcaat ggctgccggc atgcttactg atacatcaat
     7621 aagcagctta ttgttagcca ctttaatgtc aggaagctta agctctgtta gtgttttggc
     7681 tattctctct cctcttggaa gactggttga aagagccagg aatatttcca ataacccatt
     7741 aagtcaatcc ctctacactg ggcgcaccga tgagtttggc caaatagagt ttgctttacg
     7801 aatgatgcaa gctgaaacag gcgccatagt aggtcgcata ggtgatgcat caaatcggct
     7861 tagcgaacac acccgaggcc tactaaagga tattgagtca agcaatgtac ttacagttga
     7921 gcagcaggca gagacagatc aaatagcaac ggcagtaaac caaatggtgg caagcattca
     7981 agaggttgcg agcaatgcac agcatgctgc agatgcggcc ggaagagcag acactgagac
     8041 ggcatctggc cagcgtctgg tagcccacac aagccagtca atcactgccc ttgaaggtga
     8101 aattaggcaa gccgctcagg ttattcatga gcttgaaggt caaagtaacg agatatcaaa
     8161 agttcttgac gttatacgag ggatcgccga gcaaacgaac ttgttggcac tcaacgcagc
     8221 aattgaggcc gcgcgtgctg gtgagcaggg gcgtggtttt gctgttgtcg ccgatgaggt
     8281 tcgcagtctt gctgctcgca cacagcaatc gacaacggat attcaaagca tgatcagcgc
     8341 tctacaagag cgagcgcaat ccgctgttac agtcatggag caaagtagtc ggcaagcgga
     8401 cacgagtgta gctcacgcag aggaagcagc tacagctctt gatggaattg gccaactcgt
     8461 taacgaaatt accgacatga acgcgcaaat agcgactgcg gtcgagcagc agggagcagt
     8521 aagtgaag
//
