LOCUS       NZ_AP026379             8534 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Aeromonas caviae strain KAM497 plasmid pKAM497_4, complete sequence.
ACCESSION   NZ_AP026379
VERSION     NZ_AP026379.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446293
            Assembly: GCF_024347715.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 03:51:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,577
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,269
            CDSs (with protein)               :: 4,269
            Genes (RNA)                       :: 159
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 123
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 51 of 149
            Pseudo Genes (incomplete)         :: 105 of 149
            Pseudo Genes (internal stop)      :: 15 of 149
            Pseudo Genes (multiple problems)  :: 20 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026379.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM497"
                     /db_xref="taxon:648"
                     /plasmid="pKAM497_4"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<102..281)
                     /locus_tag="OCZ21_RS22725"
                     /old_locus_tag="KAM497c_44040"
                     /pseudo=""
     CDS             complement(<102..281)
                     /locus_tag="OCZ21_RS22725"
                     /old_locus_tag="KAM497c_44040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /name="SRPBCC domain-containing protein"
     gene            469..906
                     /locus_tag="OCZ21_RS22730"
                     /old_locus_tag="KAM497c_44050"
     CDS             469..906
                     /locus_tag="OCZ21_RS22730"
                     /old_locus_tag="KAM497c_44050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353700.1"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]; GO:0008080 - N-acetyltransferase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_174501508.1"
                     /translation="MIRVMRETDIPQLAQLYLKGRRQTFHWVDPSHFRLEDFGEHTRGE
                     QLWVAEQGGTPCGFVSIWGDESFVHHLYVSADWHGQGLGRALLTQGLAACRKPASLKVA
                     THNTSALAFYHRLGWQNTEETGHCEITGPWRRLIPSSTLRG"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(1065..1421)
                     /locus_tag="OCZ21_RS22735"
                     /old_locus_tag="KAM497c_44060"
     CDS             complement(1065..1421)
                     /locus_tag="OCZ21_RS22735"
                     /old_locus_tag="KAM497c_44060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_174501507.1"
                     /translation="MEPERFKRTMKARGHWQDGELTSAEPAVLVDDRGSVTLNEPAKGY
                     WVDGAPVWPAYNPVTGEDLSDQLREVLEANHKKAIILTLADAAATLKDMAKELAVERLF
                     IRPTTWQNSLVIME"
                     /name="hypothetical protein"
     gene            1730..2707
                     /locus_tag="OCZ21_RS22740"
                     /old_locus_tag="KAM497c_44070"
     CDS             1730..2707
                     /locus_tag="OCZ21_RS22740"
                     /old_locus_tag="KAM497c_44070"
                     /inference="COORDINATES: protein motif:HMM:NF023605.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="WP_110198772.1"
                     /translation="MAKKPKDITPRFGIGEWFGFNLTQLSAGERRQLAAEVLKPKKERT
                     PQPCPFQARKQDAICSKDGGVCSLRLYSYDTHQDNGRAVGVPVEGKQGDIRATCPYRFH
                     DELDVFKWVGETILGDPDPQLVGEVGFLEAGASTDSEGGDDVGRIDMVLVSSKTPDGAP
                     MNWAALEIQAVYFSGNAMKGEFEAFNDPSVDWVIFPAGRRRPDYRSSGPKRLMPQLQIK
                     VPTLRRWGKKMAVVVDRAFFDSIGEMDNVADISNADIAWFVVRFEEVEGQKRRRIVRDE
                     VRYTTLERSVEGLTGGKPVPLPVFETRITDKIVFPAPIIGPAQE"
                     /name="NotI family restriction endonuclease"
     gene            2841..3869
                     /locus_tag="OCZ21_RS22745"
                     /old_locus_tag="KAM497c_44080"
     CDS             2841..3869
                     /locus_tag="OCZ21_RS22745"
                     /old_locus_tag="KAM497c_44080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="WP_110198773.1"
                     /translation="MTTDATALQRQNRRIERLKEQGVTRATVLVHNECKPALEGLRHHF
                     VDPDKAQALASLVEQLHDKKAPTNVAQVRQLSPFRYPGGKTWLVPEVRKWLMASKVTPS
                     VFVEPFAGGAMAGLSTAAERLADHVFLGELDDDVAAVWQTIFHGKPADVEWLCKKIINF
                     DVNLENVRKILDGNPTSIRGKAFRTVVKNRMQRGGIMAAGAGLVKAGEAGRGLHSRWYP
                     ETLARRIEALQAMRERVTFEQADAFEVVQRYANDANAFFFVDPPYTAGGKKAGSRLYTH
                     NEVDHEGLFSLMASVRGSVMLTYDDAPEVRLMAELHGFRIEPVPMKNTHHQVIRELLIL
                     KP"
                     /name="DNA adenine methylase"
     gene            complement(4106..4330)
                     /locus_tag="OCZ21_RS22750"
                     /old_locus_tag="KAM497c_44090"
     CDS             complement(4106..4330)
                     /locus_tag="OCZ21_RS22750"
                     /old_locus_tag="KAM497c_44090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_110198774.1"
                     /translation="MTELERQLLKALESLEGGYKAQHAAWESAYSDLRLMFEATSTENR
                     ALRENVGSLSKQVLHLSEQVERLSQKLKP"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4331..4804)
                     /locus_tag="OCZ21_RS22755"
                     /old_locus_tag="KAM497c_44100"
     CDS             complement(4331..4804)
                     /locus_tag="OCZ21_RS22755"
                     /old_locus_tag="KAM497c_44100"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_223930706.1"
                     /translation="MSKILELAKTFEESSKQQASDIETSVKNAFEPHEKAILAALESSA
                     RRLNTAIDAQNRRWGWLVLKGWVFPLIGVAFLLGISWVVVWYQGKVIAENWVEISRQNA
                     TLEQLAEKGGKLELSTCGKEKRLCVKVDLKEQPYGRDEDTKKDEYPWMIPEGY"
                     /name="MbeB family mobilization protein"
     gene            complement(<4842..5492)
                     /locus_tag="OCZ21_RS22760"
                     /pseudo=""
     CDS             complement(<4842..5492)
                     /locus_tag="OCZ21_RS22760"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5489..5662)
                     /locus_tag="OCZ21_RS22765"
                     /old_locus_tag="KAM497c_44120"
     CDS             complement(5489..5662)
                     /locus_tag="OCZ21_RS22765"
                     /old_locus_tag="KAM497c_44120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_174501506.1"
                     /translation="MMNECGVAEYDYTLIRLPGEQGWSLRLLKDGQEVSGEVYQEHDEA
                     LSVATVWLCSES"
                     /name="hypothetical protein"
     gene            complement(5659..6000)
                     /locus_tag="OCZ21_RS22770"
                     /old_locus_tag="KAM497c_44130"
     CDS             complement(5659..6000)
                     /locus_tag="OCZ21_RS22770"
                     /old_locus_tag="KAM497c_44130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_110198770.1"
                     /translation="MTPTKRERVLKIRVTDDELARLKGLSGDERLAEWMRSQCLGNDKA
                     AGRRRTPVPKADPALLRQIAGIGNNLNQVARRVNSGEWGAVERVQVIAVLMAMERALQA
                     LSAPSTEVT"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5997..6302)
                     /locus_tag="OCZ21_RS22775"
                     /old_locus_tag="KAM497c_44140"
     CDS             complement(5997..6302)
                     /locus_tag="OCZ21_RS22775"
                     /old_locus_tag="KAM497c_44140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110198771.1"
                     /translation="MKDERDDQTLDLLPTGKRRGRPPTGKALSPAAKQAAYRARQREKT
                     VTVTLNRPDCGELEIFLRNLRDGRTSILDPEVVARLHEAVRSAWRGQLYSGNGDQK"
                     /name="hypothetical protein"
     gene            complement(7325..7711)
                     /locus_tag="OCZ21_RS22780"
                     /old_locus_tag="KAM497c_44150"
     CDS             complement(7325..7711)
                     /locus_tag="OCZ21_RS22780"
                     /old_locus_tag="KAM497c_44150"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223930707.1"
                     /translation="MVHVTYGTFNNISDGRDVIEINVIGLWFPVGMIGAGLFTIASPAV
                     ALITGRRSDSVWGRKGIIAGNFISASFAILGVISAVFFYQRMMTELDAKGYSYCRPLTT
                     FSAMGRYEVYVAKPELCVKPDKIP"
                     /name="hypothetical protein"
     gene            7703..7963
                     /locus_tag="OCZ21_RS22785"
     CDS             7703..7963
                     /locus_tag="OCZ21_RS22785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223930708.1"
                     /translation="MNQPPPNTPHYNIYEYSREKCGSLSDALIKGERINSNEWHFAGMA
                     EQLSADGYIVKGIEADEGFVLNSKRRWISWLLDDKPALNCR"
                     /name="hypothetical protein"
     gene            complement(8011..>8337)
                     /locus_tag="OCZ21_RS22790"
                     /old_locus_tag="KAM497c_44160"
                     /pseudo=""
     CDS             complement(8011..>8337)
                     /locus_tag="OCZ21_RS22790"
                     /old_locus_tag="KAM497c_44160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /name="SRPBCC domain-containing protein"
     Typing          complement(4027..5492)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.047"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcaaagctc agcttctgct cgcagaatcc ctggccgcat actttccgga aatggtgttc
       61 acagattccg gaatcactgt tcaacttcac cggaatacgc aacgttcacc cagcggggca
      121 tcgggcctga aatagatctc gaagggacca ttcggttcgg gcaccatcag gcattccggc
      181 gccaggaagc tgcgcaggcc gctctcggtt gtccaggcac gccagacatc gtcgatatgg
      241 gccggaacaa agatgttacc ttctatctgc ttgttatcca taaatcagcg agcctcaaac
      301 catccataca tgggactcag gcagtatagc cccatgggcg ataaaatgga cagaggtgtg
      361 tcacacccgt gccatgggtc ggccgttatc ctaaccatga tatttgacac tgtgtggcga
      421 tccatccgac agtgttgcca agtgataaca gtgacgaagg aaggcctgat gatcagagtc
      481 atgcgagaga cggatattcc ccaattggcg cagctgtacc tgaaggggcg gcgtcagacc
      541 tttcactggg tcgacccttc ccactttcgg cttgaggatt tcggcgagca tacccggggc
      601 gagcagctct gggtggctga acagggtggc accccttgcg ggtttgtttc catctggggg
      661 gatgagagct tcgtgcatca tctctacgtg tcggccgact ggcatggtca ggggctgggg
      721 cgggcactgc tgacccaggg gttggcggct tgtaggaagc ctgcctcact caaggtggcg
      781 acccacaata cctctgctct ggccttttat catcgcctcg gctggcagaa tacggaggaa
      841 acgggtcact gcgagatcac aggcccctgg cgtcgcttga tccctagctc gactttgagg
      901 ggttaagcct aactggacaa ccttataatt attaagccga tatttgtgcc cattaaattt
      961 aattctgacg ccattaacca atgcatggcg cattacttta tctccgttat ttttggttgt
     1021 tatcgaaata tactctttgc cttttgaagt aaatacttct gcctttactc cataatcacc
     1081 aaactgtttt gccaagtcgt aggccttata aacagccgct ccacagccag ctccttggcc
     1141 atgtctttca aggtcgctgc ggcgtcagcc agcgtcaaaa tgatcgcctt cttgtggttt
     1201 gcctccagta cttcacgcaa ctgatcgctc aggtcttcac ccgtaacagg gttgtatgca
     1261 ggccagactg gtgcgccatc gacccaatac cctttggcag gctcgttcag cgtcacgctc
     1321 ccccgatcat caactaacac cgcaggctct gccgatgtca gctcgccatc ctgccaatgc
     1381 cctcgcgcct tcatcgtccg tttgaatcgt tctggttcca tgaccacacc tcactgacta
     1441 ctcgaagccg ccattctgcc acagggagca atcgaggcca aaaaagaggc agcctaggcg
     1501 gcgattacag cgatttcggc acccacacca aggcgatgac ataccccaaa taaaaaacgc
     1561 gcctaagcgc gtttgagacg tggatccaga gaccttgtta taccattgct gcgatcacgc
     1621 catccagcaa gggaggggac accgcaacca tagatagttg taaaccgtgt tacctatttt
     1681 taggctcgcg cctcggacgt aacattccaa tatggtattt tagggaacta tggcaaaaaa
     1741 acccaaagac ataacgcctc gattcggaat tggggaatgg ttcggcttca atttgacaca
     1801 gctttccgct ggggagcgcc ggcaacttgc ggccgaagtc cttaaaccga agaaagagcg
     1861 aactccacaa ccctgtccct tccaagctcg caaacaagac gctatctgct ccaaggatgg
     1921 cggagtttgc tcactccgcc tctactccta cgatacacac caagacaacg gacgagcagt
     1981 aggtgtccct gttgagggta agcaagggga tatccgcgcc acttgtccat atcgcttcca
     2041 cgatgagctt gatgtgttca agtgggtagg agaaacaatc cttggtgacc cagacccgca
     2101 gcttgttggt gaagttggct ttcttgaagc cggcgcttcg actgacagcg agggtggcga
     2161 tgacgtagga cgcatcgaca tggttttggt tagctccaaa acgccagatg gtgccccaat
     2221 gaattgggca gctcttgaaa ttcaagctgt ttacttcagc ggtaacgcca tgaagggtga
     2281 gtttgaggcc ttcaacgatc catctgtaga ttgggttatt ttcccggcag gtcgtcgccg
     2341 tcccgattac cgaagcagtg gcccgaaacg cttgatgcca cagcttcaaa tcaaagttcc
     2401 aactctacga cgatggggta agaaaatggc cgttgtcgtt gatcgagctt tcttcgattc
     2461 aatcggagaa atggataacg tagcggatat ctccaatgca gacattgcat ggtttgttgt
     2521 tcgttttgaa gaggttgaag gccagaaacg tagacgtatc gtgcgggatg aggtcaggta
     2581 caccactctt gagcggtcgg ttgaagggtt gacgggagga aagccggttc ccttaccggt
     2641 gtttgagact cgtatcactg acaagattgt ctttcctgcc cctatcattg gcccagctca
     2701 agaatgagtt attgtttttt cgttgaagat gaccttagtc gggcatcttc aatgctttca
     2761 tttccgtgat cattcacgga agttgacggt atccctccca catagctaaa ctccgtgaat
     2821 gttcacggag aaaaataaat atgacaaccg acgcaacagc actgcaaagg cagaaccgcc
     2881 gcatagaacg cctgaaagag caaggcgtta cccgtgccac agtacttgtt cataacgaat
     2941 gcaaacctgc ccttgaagga ctaaggcatc actttgttga tcccgacaaa gcacaagctc
     3001 ttgcctcgct agtagaacaa cttcacgaca aaaaggctcc gactaatgtt gcacaagttc
     3061 gccagctcag tccgtttcgc taccctggtg gaaagacatg gcttgtacca gaagtgcgaa
     3121 agtggttgat ggcttctaaa gtaacaccgt ctgtatttgt agagccattt gctggcgggg
     3181 caatggctgg tttatccacc gctgcggaac ggttagcaga tcacgtcttt cttggtgaat
     3241 tggacgatga tgtggccgct gtctggcaaa caattttcca cggtaagccc gctgacgttg
     3301 agtggctttg taagaaaatt attaacttcg atgtgaacct tgagaacgtc cgtaagatac
     3361 ttgacggcaa cccgaccagc attcgtggaa aggccttccg tacagtcgtg aagaacagaa
     3421 tgcaacgcgg cggtatcatg gccgctggtg ctggtcttgt taaggccggt gaggctgggc
     3481 gtggcctgca ttcccgctgg tacccagaaa ctctggctcg tcgcattgag gcattacagg
     3541 ccatgcggga gcgggtaacc tttgaacagg ctgatgcctt cgaggtggtg cagcgttatg
     3601 cgaacgatgc taatgcgttt ttctttgttg atccgccgta caccgctggc ggaaagaagg
     3661 ctggctctcg gctctataca cacaatgaag ttgaccatga aggacttttc tctctcatgg
     3721 cctcggtgcg tgggtcagtg atgttgacat atgacgacgc tcctgaagtg cgcttgatgg
     3781 cagaactcca cggtttccgt attgaaccgg tgccgatgaa gaatacacat caccaagtta
     3841 tccgtgagtt gttgatcttg aaaccatgaa ataaatgcgt gacctatgtg atggtaacaa
     3901 ccacaaaaac tcaccctaag ccgactcctg cgacctcaca acgatctacc caaatgagcc
     3961 aaggtgcccc tgtcatgagg tgacaggggt tatccaccac aaagtggggc cactggcccc
     4021 actcaatcac cgtattcccg atcaccatac tctcgatact cacgcccgag gctcatacca
     4081 ccacgccgcg gcgctcgctc gatgctcagg gcttcaattt ttggctcaat cgctctactt
     4141 gctcgctcaa gtgcaacacc tgcttgctca agctccccac gttctcccgc aatgcccggt
     4201 tctcggtaga ggtggcctcg aacatcagcc gcaaatcgct gtaggcgctc tcccaagccg
     4261 cgtgctgcgc tttgtaccct ccttccaagc tctctaacgc tttcaataac tgccgttcga
     4321 gctctgtcat tcagtaaccc tccgggatca tccagggata ttcgtctttc ttggtgtcct
     4381 cgtcgcggcc atagggctgc tccttcaagt ccaccttcac gcacagccgt ttctccttcc
     4441 cgcacgtcga cagttccaat tttccgccct tctccgccaa ctgctccaat gtcgcgttct
     4501 ggcgtgagat ctctacccag ttctctgcaa tgaccttccc ctggtaccac accacgaccc
     4561 agctgatccc cagcagaaac gccaccccga tcaacggaaa gacccacccc ttcaacacca
     4621 gccagcccca cctccggttc tgggcgtcga tagcggtgtt caatctccgc gcgcttgatt
     4681 ccaacgccgc gagaatagct ttctcgtgcg gctcgaacgc gttcttcacg ctcgtttcga
     4741 tatcgctcgc ctgctgcttc gagctctctt cgaaggtctt ggccagctct aaaatcttgc
     4801 tcatacaggg ccccttttag ccggatattc cgtccccctt ccgggtcggc aatgctgata
     4861 ctcttgcggg tctcccgcac cacctcaaag ccgtgctgct tcaacgtggc caccacgtct
     4921 ccccggtcgg ccagctcacc ggccgccgcc agtgaaagca gaccgtcagt gatcgcttgt
     4981 gccgcgtcct gtttggcttt tggcaagttg ctgggggtca tcaatgcccg tttgttcaag
     5041 ggatcatccg ggtcatgcaa gcccaactgg gcgttgacca ccaccttcca cgcatcgatg
     5101 cggggccggt cggcccggtc gaaatagggt tgcagccgtt tgccactcag cagctcgata
     5161 ttcgggatca cgaagttgag ctcaagccgt tctttatccc ggtgctccac ccacaggcag
     5221 gcgtactgat cccgatccag ccccggcaat aaggcacgct cgaagctgtc catcagcttg
     5281 tccttgtcct ccctgggcaa atccgcttcc tggaacgaca gcacgccaga ggtgtacttt
     5341 ttggcgtagg ggctgctgtc gatcagcgcc tcgatgagac tcggatcgcc acggtcaagg
     5401 gtcgccccct cccgatcccg ctgcggcccc agcaggtaat cgatcgggcc gctaccgcgc
     5461 cccacgccac gggcatgaaa tttgacgatc atgactcgct gcacagccag acggtggcca
     5521 cactgagcgc ttcatcgtgc tcctggtaaa cctccccgct gacctcctgg ccgtccttca
     5581 acaaccgcaa gctccacccc tgttcgccgg gcaaccggat cagggtgtag tcatactcgg
     5641 ctacgccgca ttcgttcatc atgttacctc cgttgagggt gcagagaggg cctgcaaagc
     5701 acgctccatc gccatcagca ccgcgatcac ctggacacgc tcaaccgcgc cccactcccc
     5761 gctattcact cgccgcgcta cctgattgag gttgttgccg atcccggcaa tctgcctcag
     5821 cagggccgga tcggctttgg gtaccggcgt tcgccgccga ccggctgctt tgtcgttccc
     5881 aagacactgg ctccgcatcc attcggccag ccgttcatcg ccactcaagc ccttaagccg
     5941 ggccaattca tcgtcggtga cccggatctt gagcactcgt tcgcgtttgg tcggggtcat
     6001 ttttggtcac cgttaccgga ataaagctgc ccccgccatg ccgaccgcac agcctcatgc
     6061 agcctggcca ccacctcggg gtccaggatc gaggtgcggc catcccgtag gttccgcagg
     6121 aagatctcca gctcgccaca atccggacga tttaaggtaa cggtgaccgt tttttcccgt
     6181 tgccgtgccc ggtaagcggc ttgcttggcc gctgggctca acgccttacc ggtaggtggc
     6241 cgacctcggc gcttgccagt cggcagcaag tccaaagtct ggtcgtctcg ttcgtccttc
     6301 atccgatcac ctcaattacg gtaactgtga ccatatacct atcctgtccg ccgacctggg
     6361 aaagcccgga acggggtttt tcggggcggt ggaggcaggg ggtttcgggg gcggcacgcc
     6421 cctgaaccag cttcacagag gaaccgaaga cgcgaagcgg ctaagggccc tctgtgtatg
     6481 ctggcttaga gtcaagtttg acatagccgg aataggttgt aaccacctgt tggtgatact
     6541 atttaaagta acagtgaccg taattagagg gcttcgagga taggtagccg gtaggaggca
     6601 aaaatagatc aaggggggta atgatgcgtg ccctggggaa gcaaaaaaac gggcgaaaag
     6661 gatgttggca agccgccgca gtttgccggg ctctcctatc tttcgctgcg cttcaagata
     6721 ggagagccct cctacagacc cacatcgatt tagggtcttt tacaggataa gctcgccttg
     6781 ctccggacta tcgagactgc ttgcggcagc tccctctcac gcgctccaga gaacacaccc
     6841 cgtcccgctg cactctgctt tagcccccgc ctgttggtcg ggctgccgcg ccgtttttta
     6901 agagctggcc gtggttggtt ccccgttacg ctctacttgc agatgcccca gggttgcgag
     6961 ccctgggagg ctgtggtgcg cgattgcacg cttgggcagt atgtgagatt tccccgttct
     7021 gcccgacctt cacacccgct acggcaacaa tcacttgcgt ccaatccgag cgaatacgac
     7081 aaagcgccaa aggtgaaagc ctttggtaac agggttcgag caggggtttg atacttgaac
     7141 agtgggccgc acgcggctaa actgaggggg tacttaacgg tatccgcttc ctgctcgaag
     7201 ctgttaccca ccgggttcta aggtcgccaa accgaagata tccgggcaaa taacccacct
     7261 tacggtgggt tttttgtttc tggcgagcag gttacgccgg atctgaggat ccgatcaaga
     7321 tcatttacgg gatcttgtct ggcttcacac acaattcagg cttggccacg tagacctcat
     7381 accgccccat agcggagaac gtcgttaacg gacggcaata tgaatatcct tttgcatcaa
     7441 gctccgtcat cattctttga taaaagaata cggctgaaat tacaccaaga atagcaaacg
     7501 atgcagatat gaaattccca gcaataatgc ccttacgccc ccaaacagaa tcagaacgcc
     7561 taccagtaat taatgctaca gctggagacg cgattgtaaa caaaccagca ccaatcatgc
     7621 ctacaggaaa ccataggcca atcacattta tttcaatcac atcacgacca tctgatatat
     7681 tattaaatgt tccataagta acatgaacca accaccacca aataccccac actataatat
     7741 ttacgaatac tccagagaaa aatgcggttc cctaagtgac gccttgatca agggcgagcg
     7801 tattaatagc aacgaatggc attttgccgg gatggcagaa cagttgtcag cagatggcta
     7861 catagtaaaa ggcattgagg cagatgaagg gtttgttcta aattcaaaac ggcgctggat
     7921 ttcctggttg ctggatgaca aacccgcgtt gaactgccgc taattgaata taaaaaacac
     7981 cgccagctgg cggtgttttg atatgtcggc ttattgagcc agtgatttca gttgctgctt
     8041 gtccggcggg ttattccagt cgatggggcc gtgggtgaag cgatactgca ggcggggcag
     8101 gctggtttcc agccactctt cccgaaaata ggcgtagccc tgatcccagt cgggatcgtt
     8161 tgcccagccg ctttgcagga agtagaggcg agtaccctga ccttcaggca caaaacgcag
     8221 gctgacctga gttttttgca gacgaatatg gggcagggag ggcggaaagt tccagctgaa
     8281 gctgagcagg tcgtggggca tgacggccat cacccgacag ccttcgctac cacgtttgca
     8341 tattccggtc gaaaatgaac acctattccg gtttaaaatg aacgctgatt ccggcttgcg
     8401 tgtacaccat ttccggacac ctcagagcac gaactctgat gtcagatgaa ccgtcatgct
     8461 ccaatgacct ggatccacaa ctgccgcgct gcgcttggct gctggcttgt tcggtgagtc
     8521 gagaagatgc ctcc
//
