LOCUS       NZ_AP026378             9757 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Aeromonas caviae strain KAM497 plasmid pKAM497_3, complete sequence.
ACCESSION   NZ_AP026378
VERSION     NZ_AP026378.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446293
            Assembly: GCF_024347715.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 03:51:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,577
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,269
            CDSs (with protein)               :: 4,269
            Genes (RNA)                       :: 159
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 123
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 51 of 149
            Pseudo Genes (incomplete)         :: 105 of 149
            Pseudo Genes (internal stop)      :: 15 of 149
            Pseudo Genes (multiple problems)  :: 20 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026378.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM497"
                     /db_xref="taxon:648"
                     /plasmid="pKAM497_3"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            1..879
                     /locus_tag="OCZ21_RS22655"
                     /old_locus_tag="KAM497c_43900"
     CDS             1..879
                     /locus_tag="OCZ21_RS22655"
                     /old_locus_tag="KAM497c_43900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_009873352.1"
                     /translation="MALDIMAAFTNEPPELDFIWPGFLAGTVGALVAPGATGKSFFALE
                     AAMSIACSVAGGDLVGLAPAHTGRVVYLAGEDPQPALVRRVHAIGQHLNQSAREAIAEN
                     LMLEPIMGKRLNVMDEAHLRRVIDYSAGARLIVLDTLSRIHSLDENSNGDMAHLVSVLE
                     HIAATTGAAVLYLHHVNKGSARDGQTDQQQAARGASALIDNARWCGYVAKMTEQEAERL
                     SDRGFDRAPIGNERRGLFVRFGVSKQNYDATPLDRWYQRHTGGVLLPVELQEIKRESGN
                     KGGKGYDSAAY"
                     /name="helicase RepA family protein"
     gene            860..1852
                     /gene="repC"
                     /locus_tag="OCZ21_RS22660"
                     /old_locus_tag="KAM497c_43910"
     CDS             860..1852
                     /gene="repC"
                     /locus_tag="OCZ21_RS22660"
                     /old_locus_tag="KAM497c_43910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_009873351.1"
                     /translation="MTQQRIKPAYAKFDPAHVFDGLFVPTKGKKRGRLYVPPRRFGDSE
                     ISFQGFEQLGADDQSILLAVSAQLGIDGLMIEAKPPGEIGQQLRLALDFDNDDGRPLKT
                     KKTSLRSLLIDAGYDDPESGAALARIKESLNRMGNAQIREINHATGWDYRCNLLSSRFN
                     HKTGEIFVAVNPRLTGAVFREQHVKVSLFERNELESEAAKLLHCWLCSNIRLGRSLGNG
                     NGAHIDTLAPHVWGKAAWEEASTKVRSVRRGQLRDALDEISDRTRGLQGGMGWAIDQTS
                     SGLVLVSRPKELPFLEGEHDMRPSEYHDLMSQPPMQEPDLFDPQDWTPD"
                     /name="repC"
     gene            complement(1788..2468)
                     /gene="tetR(C)"
                     /locus_tag="OCZ21_RS22665"
                     /old_locus_tag="KAM497c_43920"
     CDS             complement(1788..2468)
                     /gene="tetR(C)"
                     /locus_tag="OCZ21_RS22665"
                     /old_locus_tag="KAM497c_43920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(C)"
                     /protein_id="WP_173782116.1"
                     /translation="MNKLQREAVIRTALELLNDVGMEGLTTRRLAERLGVQQPALYWHF
                     KNKRALLDALAEAMLTINHTHSTPRDDDDWRSFLKGNACSFRRALLAYRDGARIHAGTR
                     PAAPQMEKADAQLRFLCDAGFSAGDATYALMAISYFTVGAVLEQQASEADAEERGEDQL
                     TTSASTMPARLQSAMKIVYEGGPDAAFERGLALIIGGLEQVRLSPASSPAGRTNLVLAL
                     AAGS"
                     /name="tetR(C)"
     gene            2561..>2782
                     /locus_tag="OCZ21_RS22670"
                     /pseudo=""
     CDS             2561..>2782
                     /locus_tag="OCZ21_RS22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(3087..3839)
                     /locus_tag="OCZ21_RS22675"
                     /old_locus_tag="KAM497c_43940"
     CDS             complement(3087..3839)
                     /locus_tag="OCZ21_RS22675"
                     /old_locus_tag="KAM497c_43940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OfxX fusion product"
                     /protein_id="WP_261950820.1"
                     /translation="MRIKIKGEITAERLAEALHAAAEKYEAVRPGHKVYGANLYLTTFD
                     ADGLPFDLVDHRGEPLSITIEAKSGELVKPALTAEGEAHRQKAKEEARRQAEEAEAEAQ
                     RRHRQTLDEYEQERQKRRKKEAEARKQFEDANAITAELLKTMPERFIDELNKTVQGVWD
                     DLKPTETQGKKKGQPKAPPVFSIHADGLVLSVETWKNPRRVLNPLCTLQHGEIAPFWMH
                     EAWLEAMRRIVDLLDTLTAAPAEALESQ"
                     /name="OfxX fusion product"
     gene            complement(3836..4474)
                     /locus_tag="OCZ21_RS22680"
                     /old_locus_tag="KAM497c_43950"
     CDS             complement(3836..4474)
                     /locus_tag="OCZ21_RS22680"
                     /old_locus_tag="KAM497c_43950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6753 family protein"
                     /protein_id="WP_009873359.1"
                     /translation="MSDLDDSFAKLLGRQPSDAERQSLYRVRDALGLKNNDALWLVLMA
                     LQHYQGQYEKFPQAIAQAAKDTLVNFKVTADATVKASAEAAKADLAQAVAAAAQEVAHN
                     TSAKQMWQWAAGCIAVAFLCVGLFGWYMHSSGKDSGYQAGYGAGYTEAKDEKAAAAWAN
                     TPEGRLAYRFAQTGSLASLAKCDRPGWYVEKGVCYVKPASDGTYGWRLP"
                     /name="DUF6753 family protein"
     gene            complement(4467..5150)
                     /locus_tag="OCZ21_RS22685"
                     /old_locus_tag="KAM497c_43960"
     CDS             complement(4467..5150)
                     /locus_tag="OCZ21_RS22685"
                     /old_locus_tag="KAM497c_43960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mobD"
                     /protein_id="WP_009873358.1"
                     /translation="MAKSIYLIGGSKGGVGKSLVTMATVDYLQERGESVLLIESDTSNP
                     DVWKAYKESTETELINLDEADGWIQLVNLCDSKPDSVVVINTAARNNKGVSAYGETLNS
                     TLEELKRKLVTLWVINRQRDSLELLKEYMDAIPNADVHVVRNGHFGEEKKFELYNGSKL
                     RTAVEERGGKSVTFPDMADRVSDDIYSKRMSIAVALKELPIGNRAELTRWRNEAKKVLE
                     AVVHE"
                     /name="protein mobD"
     gene            complement(5163..5519)
                     /locus_tag="OCZ21_RS22690"
                     /old_locus_tag="KAM497c_43970"
     CDS             complement(5163..5519)
                     /locus_tag="OCZ21_RS22690"
                     /old_locus_tag="KAM497c_43970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873357.1"
                     /translation="MKYTTEQLEAIASKLREMPPVEKKKQEHSKQEAVRVLSKEIAALQ
                     KRGYTLDQISETLRGEGLSIATPTLKSYLQRAKPAKKAPVQAPGDTPPPRPADKKPADT
                     SKATFTPKPDSDDI"
                     /name="hypothetical protein"
     gene            5835..6146
                     /locus_tag="OCZ21_RS22695"
                     /old_locus_tag="KAM497c_43980"
     CDS             5835..6146
                     /locus_tag="OCZ21_RS22695"
                     /old_locus_tag="KAM497c_43980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003869.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_009873356.1"
                     /translation="MPFETQGPEPLDAVINVRLTAAEKARLKEDADLAGLSMSELVRRR
                     YFGRPIIANADAVMLKELRRIGGLLKHIHNESGGVYSKETAGALVALKDYFRKLNDDR"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            6136..6351
                     /locus_tag="OCZ21_RS22700"
                     /old_locus_tag="KAM497c_43990"
     CDS             6136..6351
                     /locus_tag="OCZ21_RS22700"
                     /old_locus_tag="KAM497c_43990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_261950818.1"
                     /translation="MIVKKVPNPKKSASKAQRIGQLTSYVRSPESESPQEKCLYAGARG
                     FMMDDPKSQTAEMIALSQEAVRITTS"
                     /name="hypothetical protein"
     gene            6348..8792
                     /gene="traI"
                     /locus_tag="OCZ21_RS22705"
                     /old_locus_tag="KAM497c_44000"
     CDS             6348..8792
                     /gene="traI"
                     /locus_tag="OCZ21_RS22705"
                     /old_locus_tag="KAM497c_44000"
                     /inference="COORDINATES: protein motif:HMM:NF041893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI/MobA(P) family conjugative relaxase"
                     /protein_id="WP_261950819.1"
                     /translation="MSWREGEQPSPEQVEEAVSIFMDELGWKDHQAIYGLHSDTDNIHL
                     HIVINRVHPETLKIVEKNRGFDIELAHKAIARIEHAQGWQREQNGRYQVLENGELGRAP
                     YDPEKPRQPDQKKRDMENRTGEKSAHRIAIEDGAAIIKQAQTWEQLHRELAAKGMRYEK
                     TGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQQVAPREPEPIKPDVPGW
                     KDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDELMRGNWKGKGEVLNAMR
                     SVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQKSPELAEQWRHRASEPQ
                     RIEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVSFVDKGKSIDIHDWRNRD
                     STLAALQLSAQKWGSFTVMGNDEYKAMCGKLAAEHGFKITNPELQESIQQERQRIQQER
                     VQAMKSEQLKQFERYAEAVGAERYRVTSIKMREDGSKQTFILDKKDGITRGFTPQEIEQ
                     RTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIRDGYQPAAVLESSPGNYQ
                     AIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSY
                     PEVRLLKAERRECIKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
                     YQRHYRDVLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRD
                     WNDYAQRTARYAYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGM
                     SL"
                     /name="traI"
     gene            8859..9083
                     /locus_tag="OCZ21_RS22710"
                     /old_locus_tag="KAM497c_44010"
     CDS             8859..9083
                     /locus_tag="OCZ21_RS22710"
                     /old_locus_tag="KAM497c_44010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="WP_043149934.1"
                     /translation="MATAHVNQRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQC
                     LLVAQADSADTAMQQFMDEALADVDGWTE"
                     /name="antitoxin MazE family protein"
     gene            9080..9406
                     /locus_tag="OCZ21_RS22715"
                     /old_locus_tag="KAM497c_44020"
     CDS             9080..9406
                     /locus_tag="OCZ21_RS22715"
                     /old_locus_tag="KAM497c_44020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303585.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_009873354.1"
                     /translation="MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTLN
                     AAPLLRVTVQPSAENGLQKPSQVMVDKAMTVLRSKVGPAFGRIDADALLEVERCLAVFL
                     GIAK"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            9403..9729
                     /locus_tag="OCZ21_RS22720"
                     /old_locus_tag="KAM497c_44030"
     CDS             9403..9729
                     /locus_tag="OCZ21_RS22720"
                     /old_locus_tag="KAM497c_44030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873353.1"
                     /translation="MKTIYTIPAPDSLGAMIEVYGEPENAWYEWRIIDGGRTVRDTGTE
                     GHSAFQGRQYGQAEIALRDALMFASGLPIDGDTPTDAASLEAGYAAKAKAEAARQGAGT
                     PGSA"
                     /name="hypothetical protein"
     ARG             2562..2806
                     /name="tet(C)"
                     /gene="tet(C)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="20.45"
     Typing          2..879
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6310..8789
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.912"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctttag acattatggc ggccttcacc aatgagccgc cagaacttga tttcatctgg
       61 cccggattct tggccggaac cgtgggcgca cttgttgcac ctggcgcaac tggcaagagc
      121 ttttttgctc ttgaagcggc catgtcaatc gcttgcagtg tggcaggcgg cgaccttgta
      181 ggactggccc cggcgcacac cgggcgcgtg gtctatctcg ctggcgaaga tccacagccc
      241 gcccttgtgc ggcgtgtcca cgccatcggc cagcacctca accagtcggc ccgcgaagcc
      301 atcgctgaga acctgatgct tgagccgatc atgggcaagc ggttgaacgt gatggacgag
      361 gcgcacttgc gccgcgtcat cgactacagc gcaggggcgc ggctgattgt gctggacaca
      421 ttgagccgca ttcacagcct cgacgagaac agcaacggcg acatggccca ccttgtttcc
      481 gtgttggaac acatcgcggc aaccaccggg gcggctgtcc tgtacctgca ccacgtcaac
      541 aagggcagcg cccgcgacgg ccagaccgac cagcagcagg cagcgcgggg cgcgtctgcc
      601 ctgatcgaca acgccagatg gtgcggctat gtcgccaaga tgacggagca ggaagccgag
      661 cgcctgagcg accggggctt tgatcgtgcg cccatcggca acgagcggcg cggccttttt
      721 gtccgctttg gcgtgagtaa gcagaactac gacgcgaccc cgctagaccg ctggtatcag
      781 cgacacaccg gcggggtgtt gctgccggta gagctacagg agatcaagcg tgagagcggc
      841 aacaaggggg gcaagggcta tgactcagca gcgtattaaa ccggcctatg ccaaattcga
      901 tcccgctcat gtgtttgacg ggcttttcgt ccccacgaag gggaagaagc gcgggcgact
      961 gtatgtgccg ccgcgccgct tcggcgatag tgaaatcagc tttcaagggt tcgagcagct
     1021 tggagctgat gaccaatcaa tcttgcttgc agtaagcgcc cagcttggca ttgacggctt
     1081 gatgatcgag gcaaaaccac ccggcgaaat aggccagcaa ttgcgccttg ctttggactt
     1141 tgacaacgat gatggaaggc cgctgaaaac caagaaaacc agtttgcgca gcctgctgat
     1201 tgatgctgga tatgacgacc cagaaagcgg ggcggcactt gccagaatca aggaaagcct
     1261 gaaccgaatg ggtaacgcgc agatacggga aatcaatcac gctaccggct gggattaccg
     1321 ctgcaatctc ctttcgagtc ggttcaatca caagactggg gaaatctttg ttgccgtcaa
     1381 tccgcgcctg accggggcgg tttttcgaga gcaacacgtc aaggtttcgc ttttcgagcg
     1441 caacgagctg gagagtgaag ccgccaagct gctgcactgt tggctgtgct caaacatccg
     1501 gcttggccgt tcgcttggca atggcaacgg ggcgcacatc gacacgctag cgccccacgt
     1561 atggggcaag gcggcgtggg aagaagcatc aacgaaggtt cgatcagtca ggcggggtca
     1621 actacgcgat gctttagacg aaatctcaga ccgcacacgc ggactgcaag gcggtatggg
     1681 atgggccatc gaccagacca gcagcgggct tgtgctggta agtcgtccca aggaactgcc
     1741 gttccttgag ggtgagcacg acatgcgccc cagcgaatac cacgacctca tgagccagcc
     1801 gccaatgcaa gaaccagatt tgttcgaccc gcaggactgg acgccggact gagccgcacc
     1861 tgttcaagac cgccgatgat gagagccagg cctcgctcga atgccgcgtc cggaccgcct
     1921 tcgtagacga ttttcatcgc gctctgtagg cgcgccggca tcgtagacgc tgaggtggtc
     1981 aactgatctt cgccccgctc ctcggcgtct gcctcgctag cttgctgctc aagaacagcg
     2041 ccgacggtga agtagctgat tgccatcaac gcataggtcg cgtcacctgc cgaaaagcca
     2101 gcatcgcaaa ggaagcgaag ctgcgcgtcg gctttttcca tctgcggcgc ggctggccgc
     2161 gtcccggcat gaatacgcgc gccatcgcga taagcgagca acgcccgtcg aaaactgcat
     2221 gcattgccct tcaggaacga acgccagtcg tcgtcatccc ttggcgtcga atgcgtgtga
     2281 tttatcgtca gcatggcttc ggcaagtgcg tcgagcaacg cacgcttgtt cttgaaatgc
     2341 cagtagagcg ctggctgttg caccccgagg cgctcagcca gtcggcgcgt cgttagacct
     2401 tccatgccca cgtcgttaag cagttcgagc gcggttcgga tcacggcctc gcgttggagc
     2461 ttgttcattc gcgaattctc atgtttgaca gcttatcatc gataagcttt aatgcggtag
     2521 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct
     2581 catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt
     2641 actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt
     2701 gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc
     2761 cgaccgcttt ggccgccgcc cagtccaccc ccggcgcagg tggtaaccac ccccggcgca
     2821 ggtggtaacc acccccggcg caggtggtaa cctgacaagc cacaaaaacg cccgcaaacc
     2881 cttgtcagta ctgagttttc gcgtttttcc aaaattccgg tctaagaagt tctaaagaac
     2941 ttctaccatg cgcggctttc agccgccgct ttaggcgtcg tctgaagcct tgccttcggg
     3001 cttcgccctg cgcgggctaa agcccttgca gacacacccc cgcctccgcc ccgccgctac
     3061 ccccttcctt gcaggcgaaa acgcctctac tggctttcta gggcctctgc cggggctgcg
     3121 gtgagcgtat ccagcagatc gactatccgc ctcatggctt ctaaccacgc ttcgtgcatc
     3181 cagaatgggg ctatttcgcc gtgctggagt gtgcaaagcg gattgagaac ccggcgcggg
     3241 ttcttccatg tctcgacgga aagcaccagc ccgtccgcat ggatggaaaa gactggtggc
     3301 gctttcggct ggcccttctt cttgccttgc gtttcggttg gcttgaggtc gtcccaaacg
     3361 ccttgcacgg ttttgttcag ttcgtcgatg aagcgttccg gcatggtttt caagagttcg
     3421 gcagtgattg cgttcgcgtc ctcaaattgc tttcttgctt cggcttcctt tttccgtcgt
     3481 ttttgccgct cctgttcgta ttcgtctaag gtttgcctgt gtctgcgctg tgcttcagct
     3541 tccgcttctt cagcttgcct tcgtgcttcc tctttggctt tctgtcggtg tgcttcaccc
     3601 tctgctgtga gtgctggttt taccagttcg ccagacttgg cctcgatggt gatcgaaagc
     3661 ggttcgccgc gatggtctac cagatcgaac ggcagaccat cagcatcgaa ggtggttagg
     3721 tagagattgg ccccatagac cttgtgcccc ggcctcacag cttcgtattt ttctgctgct
     3781 gcgtgcagtg cttccgctag gcgttctgcc gtgatttctc ctttgatctt gattctcatg
     3841 gcaacctcca tccgtaggtg ccatccgatg cgggcttgac gtagcacacg cctttttcga
     3901 cgtaccaccc cggcctatcg cacttcgcca agctggcgag gcttcccgtt tgcgcgaagc
     3961 ggtaggccaa gcgcccctct ggcgtgttgg cccatgccgc cgccgctttc tcgtccttgg
     4021 cttcggtgta tcccgcgccg tatccggcct gatagcccga atccttgccg ctgctgtgca
     4081 tgtaccagcc gaacaggccg acgcacagga aggccaccgc gatgcagccc gccgcccact
     4141 gccacatctg ctttgccgat gtgttgtgcg cgacctcttg cgctgctgct gctaccgctt
     4201 gcgccaaatc cgccttggct gcttccgctg atgctttcac ggttgcatcc gccgttacct
     4261 tgaaattgac caaggtatct ttggccgctt gtgctatcgc ttgcgggaac ttctcatact
     4321 ggccttggta gtgttgcaag gccatgagca ccagccacag cgcatcattg tttttcaggc
     4381 cgagcgcatc ccgaacccgg taaagacttt gccgctccgc gtcactaggt tgcctgccga
     4441 gcagctttgc aaagctgtcg tctaggtcac tcatggacta cggcctccaa tacctttttg
     4501 gcctcgttgc gccaccgcgt aagctccgca cggttgccaa tgggcagttc cttgagtgct
     4561 accgcaatgc tcatgcgctt gctgtaaatg tcatcgctca cacggtcggc catgtccggg
     4621 aatgtcaccg atttcccgcc gcgttcctct accgccgtgc gcagcttgga gccgttgtaa
     4681 agctcgaact tcttttcttc cccgaagtgt ccattgcgta ccacatgaac atccgcattc
     4741 ggaattgcgt ccatgtattc ctttagcagt tcgaggctat cccgttggcg gttaatcacc
     4801 cacagcgtga ccagcttgcg cttgagttct tcaagcgtgc tattcagtgt ttcaccgtag
     4861 gcgctcacgc ccttgttatt ccgtgctgcc gtgttgatta ccacgacgct atccggcttg
     4921 ctatcgcaca ggttcactag ctgaatccat ccgtccgcct catccagatt gataagctcc
     4981 gtttccgtgc tttctttata ggctttccat acgtccgggt tagaggtgtc agactcaatc
     5041 agcaagacgc tttcgccgcg ctcttgcagg taatcaaccg ttgccatcgt aacaagcgac
     5101 ttgccaacgc cgcccttgct gccaccgatc aggtaaatgc ttttcgccat gttgcttact
     5161 ccttaaatgt cgtcgctgtc aggtttcggg gtgaatgtcg ccttcgatgt atccgcaggc
     5221 ttcttatcgg cggggcgggg cgggggtgtg tctcccggtg cttgcaccgg ggcttttttc
     5281 gctggctttg ctctctgcaa gtagctcttg agcgttgggg ttgcgatgct cagaccttcg
     5341 ccgcgcagcg tctcggaaat ctggtcaagc gtgtatccac gcttttgcaa agcggcaatc
     5401 tctttggata gcacgcgcac ggcctcttgc ttgctgtgct cctgcttctt cttttcgact
     5461 ggcggcatct cacgaagctt tgaagcgata gcctctagct gctctgttgt gtatttcatt
     5521 ctgctaactc ctttgctcac tggttgcgga gttgacagca tagcgccttt tatgcttgtt
     5581 gcaaagtgcg tcccgtgcac ctaaactgct gatatgatag tagagtggtt gagcaataca
     5641 ggatgcggat tgtcatacac atgctacgca tgttcctgcc aatccccgcc tgcccattcg
     5701 gcgcttcgct tctcacggtc tggctcatcc tgcaaggggc aagccccttg accccggaca
     5761 gtgcggcgct ttcgcttctt actgtccaag acacaccccg cccccgcccc cgcccccggc
     5821 gagaaggaga catgatgcca tttgaaacgc aggggccgga accgctggac gccgttatca
     5881 atgtgcggct gaccgcagcg gagaaggcca gattgaaaga ggacgccgac cttgccgggt
     5941 tgagcatgtc cgagcttgtc cgccgccgct acttcggcag gccgatcatt gccaacgccg
     6001 acgccgtgat gctcaaggag ctgcggcgca tcggtggatt gctgaagcac atccacaacg
     6061 agagcggagg cgtttacagc aaggaaacag ccggggcgct tgttgcgctc aaggactact
     6121 tcaggaagct aaacgatgat cgttaagaag gttccgaatc cgaagaagtc cgcgagcaag
     6181 gcgcagcgca tcggccagct taccagctat gtccgttcgc cggagtcgga gagtccgcag
     6241 gaaaagtgtc tgtatgccgg ggcgcggggt ttcatgatgg acgaccccaa gagccagacg
     6301 gcagagatga ttgcactctc acaagaagcc gtgcgaatca ctacgtcttg agttggcgcg
     6361 aaggcgaaca gccaagcccg gagcaagtag aggaagcggt tagcatcttc atggatgagc
     6421 ttgggtggaa agaccaccaa gccatctatg gccttcattc cgacacggac aatatccacc
     6481 tgcacatcgt catcaatcgg gtgcatcccg aaacgctcaa gattgttgag aagaaccgtg
     6541 gatttgatat tgaactggca cacaaagcca ttgcacggat tgagcacgcc caaggctggc
     6601 agcgtgagca aaacgggcgc tatcaggtgt tggagaacgg ggagctaggc cgagcgccat
     6661 acgacccgga aaagccacgc cagcccgacc agaagaagcg ggacatggag aaccgcaccg
     6721 gggagaagtc agcccaccgc atcgccatcg aggacggcgc agccatcatc aagcaggcgc
     6781 agacgtggga gcagttgcac cgcgagttag cagccaaggg gatgcgctac gaaaagaccg
     6841 gcagcggggc gaccgtgttt gtcggggatg ttggcgtgaa ggccagcgat gtagaccgca
     6901 acgccagcct tgccaagatg cagaagcggc taggtgaata ccagccagcg ccgcagcgtc
     6961 agcaggtagc gccgcgcgag ccggagccga tcaagccaga cgtgccggga tggaaggact
     7021 acatcaccgg gcgcaaggca cactacgccg agaagaacgc ggacaagctg gcacaggaca
     7081 agcggcagga gcaggagcgc aagcaacttg ccgagcagca gaaggcccgc cgcgatgaac
     7141 tcatgcgcgg aaactggaaa ggcaagggcg aagtgctcaa cgccatgcgc agcgtgattg
     7201 ccgccgagca ggccgcagag aaagcggcct tgaaggagaa gcaccagaag gagcgcgagc
     7261 agcaccggca gcggttccga ccctatcccg acctggagca gtggcagcgg atgcagaaaa
     7321 gcccggagct tgccgagcag tggcgacacc gggccagcga gccgcagcgc atcgagggcg
     7381 accgcagcga gccgccgacg ccgcgagaca ttcgcgccta tcagcctgaa atcgtgggcc
     7441 agcaggtgca ttacagccgg aaggaagaag cgggccgggg cgggggtgtg tctttcgtgg
     7501 acaagggcaa aagcatcgat attcacgact ggcgcaaccg ggacagcacg cttgccgcgt
     7561 tacagctatc ggcgcagaag tggggcagct tcaccgtgat ggggaacgac gaatacaagg
     7621 ccatgtgcgg gaagttagca gcagagcacg gcttcaagat cacaaatcca gagcttcagg
     7681 agagcatcca gcaggagcgg cagcggatac agcaggaaag ggtgcaggcg atgaaatcgg
     7741 agcagctaaa gcagttcgag cggtacgcgg aagcggtagg cgcagagcgt taccgggtaa
     7801 cgtctatcaa gatgcgcgag gacggcagca agcaaacctt catcctcgac aagaaggacg
     7861 gcatcacgcg gggcttcaca ccgcaggaga tcgagcagcg cacgccggag atgcagcgcc
     7921 tacagcgccg gggcgaaaac ctctactaca cgccgctatc ggacaagaag catcacatcc
     7981 tcatcgacga catgaaccgg gagaagctgg agcggcttat ccgcgacggc taccagcccg
     8041 ccgccgtgct tgaatccagc cccggcaact atcaggccat catcaccgtg ccgaagctgg
     8101 ggacggccca cgataaggac gtgggcaacc ggctgagcga tgccctgaac cgtgaatacg
     8161 gcgacccgaa gctatcggga gccatccacc cgcaccgtgc acccggctac gagaaccgca
     8221 agcccaagca ccagcgggag gacggcagct atcccgaggt gcgcttgctc aaggccgagc
     8281 gccgggagtg catcaaggcg ctggccttgt ccagccagat cgacgccgag tatcagcggc
     8341 aagcggcctt gaaggcgcag cagcccgagc gcagcaaagc caagcccgcc ttggagcttg
     8401 cagcggccag cggcagcgcg atcgacgcct accagcggca ttatcgcgac gtgctcaagc
     8461 ggcagcgtgg cggcgaggtg gacttgtccc gcttggattc catgattgcc gtgcgtatgc
     8521 gcgtcaccgg ccacgatcaa gccgccatcg agggcgctat ccgccagtgc gccccggcca
     8581 cccggcagaa ggacgagggc cgcgactgga acgactacgc gcagcgcacc gcccgctatg
     8641 cctacagcgc cgcaggcgac cgccaagccg ccgagcttgg gaagtaccgc cagcagtggg
     8701 agaagctgga agggcgcgag cctgtacgcc agcaggagca ggccaaggcg cagaagatcg
     8761 agcgcgataa ctcgccgggg atgagtcttt aaatacctgt tgcatgtaac ttgcgaggtg
     8821 cgtacaatgt tgcatgtaac ttaattggag gcttggaaat ggcaacagca cacgttaatc
     8881 agcgggttca gaagcatcgc gatgcattac gcatggcggg gcttcgtccg gttcaaatct
     8941 gggtgccgga tacgcgccgc cccgacttcg cggaggagtg ccgccgccaa tgcctgcttg
     9001 tggctcaggc cgacagcgcc gacaccgcca tgcagcagtt catggatgaa gccttggcag
     9061 acgtggacgg ctggacggaa tgatgcgcgg cgaccttgtg accatcgcca tgcagggcga
     9121 ctttggcaag ccacggcctg cgcttgtggt tcaggccaac cagttcagcg agcacggcag
     9181 cgttaccgtg ctgcctgtca ccagcacact taatgccgcg ccgttgctgc gcgtcacggt
     9241 tcagccgagc gcagagaacg gtttgcagaa gccatcgcag gtgatggtcg acaaggcgat
     9301 gacggtgctg cgcagcaagg tagggccagc tttcgggcgc atcgacgcgg acgcactgct
     9361 tgaggttgag cgttgcttgg ccgtgttctt ggggattgcg aaatgaaaac gatctacacc
     9421 attcccgccc cggattcctt gggcgcgatg atcgaggtct acggcgagcc ggaaaacgct
     9481 tggtacgagt ggcgcatcat cgacggcggg cgcaccgtgc gggacaccgg cacggaaggc
     9541 cacagcgcct ttcagggacg ccagtacggg caagcagaga ttgccctacg tgatgcgctc
     9601 atgttcgctt ctggcctgcc catcgacggc gacacgccga ccgatgccgc cagtctggaa
     9661 gcgggctatg ccgccaaggc gaaggcagaa gcggcgcggc agggtgcggg aacacccggc
     9721 agcgcctaac cataaccgtt ctacacagga gaacagc
//
