LOCUS       NZ_AP026373             5009 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Aeromonas caviae strain KAM471 plasmid pKAM471_3, complete sequence.
ACCESSION   NZ_AP026373
VERSION     NZ_AP026373.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446292
            Assembly: GCF_024347695.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 02:42:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,435
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,123
            CDSs (with protein)               :: 4,123
            Genes (RNA)                       :: 160
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 124
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 33 of 152
            Pseudo Genes (incomplete)         :: 116 of 152
            Pseudo Genes (internal stop)      :: 13 of 152
            Pseudo Genes (multiple problems)  :: 7 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026373.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM471"
                     /db_xref="taxon:648"
                     /plasmid="pKAM471_3"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(join(4204..5009,1..139))
                     /locus_tag="OCX46_RS22115"
     CDS             complement(join(4204..5009,1..139))
                     /locus_tag="OCX46_RS22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_261921579.1"
                     /translation="MTNLAQLDLFRERLPRKPYYTDELTTGLHIADIARALGSRYIQPN
                     GPHKHWLVFDVDHSAATLSWDDVGAPAPNITATNRDNGHAHLIYGLEIPVRTAPDGKVA
                     PLRYAAAIEAALRELLGADLGYSGLICKNPLHSHWLVQVWEPQTYTLDWLADYLDLSPY
                     NGRKHLPAYGLGRNCTLFEKTRLWAYKAIRQGWPGFDTWQAAVIERASGYNVQFEQPLP
                     ANEVRHTAKSIAKWTHRNLTPAGFSAVQAARGAKGGRIGGKVSKGGGRPSKAAELLPEV
                     LRLKEMGYSNRDIAEDLQISAGSVSNYLRRDHE"
                     /name="replication initiation protein"
     gene            complement(290..598)
                     /locus_tag="OCX46_RS22120"
                     /old_locus_tag="KAM471c_42560"
     CDS             complement(290..598)
                     /locus_tag="OCX46_RS22120"
                     /old_locus_tag="KAM471c_42560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_202212061.1"
                     /translation="MQFTIYRNKGNARDYPYLLNVQSDIIGELHTRMVIPLVSLADFPG
                     RPAQRLNPVITVEGGKYLVLTHEMAAVRLAQLGEEVTDVQESRQLIKGAIDFLLDGV"
                     /name="CcdB family protein"
     gene            complement(598..837)
                     /locus_tag="OCX46_RS22125"
                     /old_locus_tag="KAM471c_42570"
     CDS             complement(598..837)
                     /locus_tag="OCX46_RS22125"
                     /old_locus_tag="KAM471c_42570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_202212060.1"
                     /translation="MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLATALQHEING
                     SEAERWKRENRAAIQTLNRMVEERGLFSDDHRTF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            complement(929..1411)
                     /locus_tag="OCX46_RS22130"
                     /old_locus_tag="KAM471c_42580"
     CDS             complement(929..1411)
                     /locus_tag="OCX46_RS22130"
                     /old_locus_tag="KAM471c_42580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202212059.1"
                     /translation="MRMKLTGLLCCVALTGCATAYQPYSYFGGGGYSDVQLAENVFQVT
                     VEANGFTTSTEAADLALLRSADLTLERGFKYFIIGSTADHSYSAGYTTPSTTTLNATSY
                     GNTIYGTAQTTGGQTYVFNFPTPSMTITCFSDQPALNGTVYDAAITSRSLRAKLGI"
                     /name="hypothetical protein"
     gene            complement(1592..1900)
                     /locus_tag="OCX46_RS22135"
                     /old_locus_tag="KAM471c_42590"
     CDS             complement(1592..1900)
                     /locus_tag="OCX46_RS22135"
                     /old_locus_tag="KAM471c_42590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_223918476.1"
                     /translation="MTELERMLLDRLERIETAHRQQTAALEQQLQQQARSLNELQTACT
                     SALASCGTLCSELQHEFETLRNGVDRSNRATTTALGSLSRSVNDLSKALDALHRAQR"
                     /name="mobilization protein"
     gene            complement(1901..2437)
                     /locus_tag="OCX46_RS22140"
                     /old_locus_tag="KAM471c_42600"
     CDS             complement(1901..2437)
                     /locus_tag="OCX46_RS22140"
                     /old_locus_tag="KAM471c_42600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="WP_223938704.1"
                     /translation="MEHLGPQKTRLSERLQQQQEQALGEVETLAKAVLEALKNDLQQRV
                     NGELDTTVTAIRKGIEGLPKQITDSSQPLQAALEQAQSEANTLKLVSSVWLKSLLIGLC
                     TAVGIGLGSWGVLSWMGSEVQRLNSEISSAKATLAQLPKGVSYAKGEDGKGYILAPSMG
                     TPFETKSGKMAVEVN"
                     /name="DUF1640 domain-containing protein"
     gene            complement(<2466..3152)
                     /locus_tag="OCX46_RS22145"
                     /pseudo=""
     CDS             complement(<2466..3152)
                     /locus_tag="OCX46_RS22145"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3142..3552)
                     /locus_tag="OCX46_RS22150"
                     /old_locus_tag="KAM471c_42620"
     CDS             complement(3142..3552)
                     /locus_tag="OCX46_RS22150"
                     /old_locus_tag="KAM471c_42620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_202212057.1"
                     /translation="MSKAPRTTTVSLRIDPATAAEWRERAAAAGLSVSDWIRGGVDAGQ
                     QTNLPTPRQRPVRVRDTSNDADPVLMRQLAGMSNNLNQIARALNECRLAGSPVQLVEVL
                     ALLRSIEAEAGRLLPQLPPPPKPTAKEASDAH"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3762..4052
                     /locus_tag="OCX46_RS22155"
                     /old_locus_tag="KAM471c_42630"
     CDS             3762..4052
                     /locus_tag="OCX46_RS22155"
                     /old_locus_tag="KAM471c_42630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202212056.1"
                     /translation="MKDTKDQGTGDMFGTKRGRGRPKTGAAKSGAERQAAYRAKQQDNN
                     VTVTINRALMEGLAAQMQAIRDGGSAVVLTQEQAGEILRALRTAETKQLRG"
                     /name="hypothetical protein"
     Typing          complement(1534..3152)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.741"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcccgttcgg ctggatgtag cgtgagccaa gggcacgggc aatatccgca atgtgcaggc
       61 ccgtggtgag ctcatcggtg taatagggtt tccggggcag gcgctcccgg aacagatcaa
      121 gctgggctag attcgtcaat gaccaccgcg cttactggcg gcttggggtt gtctcaagtg
      181 atccatgtgg tatcattttt ccataggttc gcgcctagcc ccgaatgctc tgagtcttcc
      241 gccaagttga acagcagagc ttcggggttt gtcttttctg gacagtacgt tacacgccat
      301 ccagcaggaa atctattgcc cccttgatga gctgacgcga ctcctgaaca tcggtcactt
      361 cctcccccag ctgcgccagt cgcaccgccg ccatttcgtg ggttaacacc agatacttgc
      421 caccctcgac ggtgatcacc ggattaagtc gctgtgcagg ccgtccgggg aagtccgcca
      481 gcgagaccaa ggggatcacc atccgggtgt gcagttcgcc gatgatgtcg ctctgcacat
      541 tcagcaaata ggggtagtcg cgggcattgc ccttgttgcg gtagatcgtg aactgcatca
      601 aaacgtcctg tgatcgtcag aaaacaggcc gcgttcttcg accatccggt taagagtctg
      661 tattgcggct ctgttctcac gcttccagcg ctcggcctct gagccattga tttcatgctg
      721 tagcgcggta gccagagtgg cggacagatt gatccccgcc gctctggctc gttccagcaa
      781 gctgggctcc agtgtgacgc tgaccgttct cttactgcgc attgctgctg cgaccatgat
      841 gacctccaat aaattaacac gtgctatagc acgtgttaaa tggtatcacg cccctgcaac
      901 gggtgcgact agcgcagcac ggccgacctt atattcctag ctttgctcgc agggatctgg
      961 atgtgattgc agcatcataa acggttccat ttaatgctgg ttgatcagaa aaacaggtga
     1021 ttgtcataga cggtgttggg aaattaaata cataggtttg cccaccagtt gtttgcgctg
     1081 ttccgtaaat agtattgccg tagctagtgg catttaatgt ggtcgttgac ggtgttgtat
     1141 atcctgccga gtacgaatga tcagctgtag aaccgataat gaaatatttg aatcctcttt
     1201 caagcgttag gtcagctgat cgcaacaaag caaggtcagc ggcctcggtg ctggtcgtaa
     1261 atccatttgc ttctacggtt acctggaata cattttctgc aagctgcacg tcagagtacc
     1321 caccgccgcc gaaatagctg tagggctgat aggctgttgc gcaaccagtc aacgccacgc
     1381 aacataacaa ccccgtcaat ttcattctca tttaatgttc cttacgttga aggctaattt
     1441 tcagcgacca gtgtgccaaa gcattaaccc tgaaactgat cattcaatcg cacttcaatc
     1501 tacaaattta gtgtcacagt gacgacatat cacatgctca atccatgatc tagccgctgc
     1561 tcttttcgca gctccacagc cttgtcggca atcaacgctg cgccctgtgc aaggcgtcca
     1621 gcgccttgct caagtcgttg acactgcggc tcaagctgcc aagcgctgtc gtggtcgctc
     1681 tgttcgaccg atcgacgccg ttccgcagtg tctcgaactc gtgctgtagc tcgctgcata
     1741 gcgtcccgca gctcgccagc gcgctggtgc aagcggtctg caactcgttc aaagagcgcg
     1801 cctgctgctg caattgctgc tccaaagccg ctgtttgctg ccggtgcgcc gtctctatcc
     1861 gctccagcct gtcgagcagc atccgctcca gttcggtcat tcagttcacc tccacggcca
     1921 tcttgccgct cttcgtttcg aatggtgtcc ccatgctggg ggcgaggatg tagcccttgc
     1981 catcctcgcc cttggcgtag ctcaccccct tgggaagctg cgccagcgtg gctttcgcgc
     2041 tgctgatctc gctgttcagc cgctgcactt cgctgcccat ccagctcaac accccccaac
     2101 tccccagccc gatccccacg gcggtacata gcccgatcaa cagggatttg agccagacgc
     2161 tggagaccag cttgagcgtg ttcgcttcgc tctgggcctg ctccaatgcc gcctgcaacg
     2221 gctgggacga gtcggtgatc tgcttgggca agccctcgat ccctttcctg atagcggtca
     2281 cggttgtatc cagctcgccg ttcacacgct gctgcaagtc gttcttgagc gcttcgagca
     2341 ccgctttcgc taacgtctcc acctcgccca atgcctgctc ttgctgctgc tgtagcctct
     2401 cgctcaagcg tgttttttgc ggtccaagat gctccataga gttccccttt caaccggacg
     2461 gccttcgggt agccctccgg cttgatgctg atgaagtccg ctcccgtgcg cgtgatctcg
     2521 cacccgagcg atgtctgtag gtattcccgc agctcggcgg cattggtgat tacaccctgc
     2581 acggcagcac cgatcaggtg atcggtgatc tgttttttga tggcggtctt gtcgttgttg
     2641 cccttgtacg cctcgaaatt tggccgcagc aaccgcgccc tgagcgggtc atctggccgc
     2701 gcccagccct tttcgtggtt gtgtttgtcg cgcacggcgt cgaagtcttt gcgttgacct
     2761 ggtggggctg gattgaaaga cttccctgtg cccaggtgaa cgcgggggat catgatgtgg
     2821 agatgtaccc cgccatccgg ttcttcatgc tgaacggctg tcaggtggac gtcctcccga
     2881 tccagtccgg caaaggccag cgcttcgaag tcgtccagta ccgcgctgat ttcctccggc
     2941 cgcggcgctt cttcaacagc ccatgagatc gccgcactgg aatagcgatg ctggaacggc
     3001 aacgagtcag ctaccgccgc gaacagcacc ggatcccccc gcaggaccgt cacaccggcc
     3061 cgttctgccc cggtgtggtc gagcggcccc atcaggtagg ccgaggcacg ggcagcactg
     3121 cccgtgccgt gagccaagaa tttaatgtgc atcgctggcc tccttggcgg tcggtttggg
     3181 gggcggcggc aactggggca gcaagcggcc tgcctctgcc tcgatgctgc gcagcagggc
     3241 cagcacctcc accaattgca ccggcgagcc ggccagccgg cactcgttca gggcgcgggc
     3301 aatctggttg aggttgttgc tcatcccggc caattggcgc atcaacaccg gatcggcgtc
     3361 gttgctggtg tcacggacac ggacagggcg ctgacggggc gtgggcaaat tcgtctgctg
     3421 gcctgcatcc accccccccc tgatccagtc cgacacactg agcccggcag cagcagcccg
     3481 ctcgcgccat tcggcggcag tggccggatc gatccgtagg ctgacggtgg tcgttcttgg
     3541 agctttggac acgatgcctc cggcggtggt gtgtggggga ttgaaggggc aacgcccctc
     3601 accagccccg tgttactggg agtccgcgaa gcgggcgaaa cagtaatacg ggtaggctgg
     3661 ctaaatacca gtacagcaaa aaaagagggg ggcggcaact tgctaattat cgtcacagtg
     3721 acacataatc aattaccgtt acagtgacag gaaatagcac catgaaggac accaaggatc
     3781 agggtactgg ggatatgttc ggcaccaagc gggggcgggg tagacccaag accggagcag
     3841 ccaaatccgg ggcagaacga caagcggcat accgggcaaa acagcaagac aataacgtta
     3901 ccgtgacgat aaatagggcg ctgatggagg ggctggctgc ccagatgcag gcgatcaggg
     3961 atgggggatc ggcggtggtg ctgacacagg agcaagccgg ggagatcctg cgggcactgc
     4021 gaacagcaga gaccaaacag ctccggggat aacagctact gcccacaggc caaacacacg
     4081 gcctgatgga cagccctacg ggatcgcagt tacccccctc gccaagccgc tctctatagg
     4141 gactctttta aggcttaacc aaccccctgc gggggctatt atctgataag gctttgtttt
     4201 tgatcactca tgatcacggc ggaggtaatt ggacacgcta cctgccgata tttgcagatc
     4261 ctcggcaatg tcgcggttgc tgtagcccat ctctttgagt cgtagaacct ccggcagcag
     4321 ctctgccgct ttgctgggcc tgccccctcc cttcgatacc ttgccgccaa tgcgcccgcc
     4381 cttagcccct ctggcagcct gcacggcgct gaatcctgcg ggagttaggt tccggtgcgt
     4441 ccacttggcg atgctcttgg ctgtatgcct aacctcgttg gccgggagcg gctgctcgaa
     4501 ctggacgttg tacccggatg cccgctcgat cacggcggcc tgccaagtat cgaaccccgg
     4561 ccacccctgc cgaatggcct tgtaagccca caagcgcgtt ttctcgaaca gggtgcagtt
     4621 acgcccaagt ccataggccg gaagatgttt gcggccattg taggggctca aatcgaggta
     4681 atcagccaac caatctagcg tataggtttg aggttcccag acttgcacca gccaatggct
     4741 gtgcaggggg ttcttgcaga tcaaaccgga gtagcccaga tccgccccca gcagttcccg
     4801 aagtgccgcc tcgatggcag cggcataacg caacggggcc accttgccat ccggggctgt
     4861 gcggacaggg atttccagcc cgtagatcag atgggcatgg ccgttatccc tgttcgtggc
     4921 cgtgatgttg ggcgccgggg cacctacgtc gtcccaggat agagtcgcgg cggaatggtc
     4981 tacgtcaaac acgagccagt gcttgtgcg
//
