LOCUS       NZ_AP026372             8281 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Aeromonas caviae strain KAM471 plasmid pKAM471_2, complete sequence.
ACCESSION   NZ_AP026372
VERSION     NZ_AP026372.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446292
            Assembly: GCF_024347695.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 02:42:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,435
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,123
            CDSs (with protein)               :: 4,123
            Genes (RNA)                       :: 160
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 124
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 33 of 152
            Pseudo Genes (incomplete)         :: 116 of 152
            Pseudo Genes (internal stop)      :: 13 of 152
            Pseudo Genes (multiple problems)  :: 7 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026372.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM471"
                     /db_xref="taxon:648"
                     /plasmid="pKAM471_2"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            1..513
                     /locus_tag="OCX46_RS22070"
                     /old_locus_tag="KAM471c_42470"
     CDS             1..513
                     /locus_tag="OCX46_RS22070"
                     /old_locus_tag="KAM471c_42470"
                     /inference="COORDINATES: protein motif:HMM:NF015076.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_261921577.1"
                     /translation="MVKQAIQADEFVERLPLRPYCTDDPAQGLHIRPQATALAFRHIQH
                     NPPPHVSCIVFDVDRKPYEQRREGYQEWRERGLPAPHWIAINPENGNYHLGYLLAAPVA
                     RTNAARLKPLRYLAAIEHVLAKKLGADMGYVGLITKNPYTAIGGRYGITMSPIPSTISR
                     SSAQMLI"
                     /name="replication initiation protein"
     gene            641..988
                     /locus_tag="OCX46_RS22075"
                     /old_locus_tag="KAM471c_42480"
     CDS             641..988
                     /locus_tag="OCX46_RS22075"
                     /old_locus_tag="KAM471c_42480"
                     /inference="COORDINATES: protein motif:HMM:NF020291.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_261921578.1"
                     /translation="MGQCGVRAACESANAFGREQGGPLPHSEIKATAKSIARWVWNRFT
                     PAGFSQVQAHRGAKGGRISKRPTKNGKARADLLPEVLELKAQGYSNRDIAEDLGIGSAS
                     VSRYLSKISPE"
                     /name="LuxR C-terminal-related transcriptional regulator"
     gene            1319..2869
                     /gene="mobQ"
                     /locus_tag="OCX46_RS22080"
                     /old_locus_tag="KAM471c_42490"
     CDS             1319..2869
                     /gene="mobQ"
                     /locus_tag="OCX46_RS22080"
                     /old_locus_tag="KAM471c_42490"
                     /inference="COORDINATES: protein motif:HMM:NF015354.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_167557709.1"
                     /translation="MAIYHCSVKTFSRRNGQSSTAAAAYRSGEKLLDENTGELHDYRRK
                     GGVISATIILPSHAPAWASDRARLWNEAERAETRINSTVAREFEIALPHELSPNDRERL
                     AHEFARELVEKHGFAADVAIHAPPEFQKGGTGKVIDNQNHHAHILLSTRRLGPNGFTEK
                     TREFDARSNERDDDSYLFGPALIEHWRERFALLQNLYLARAGVAAQVDHRSNKARGLDA
                     VPTRHLGPAVVGFERRMGIDSEKRQREALAVRAVDERVQELDAEVRGELAELDAQEAVI
                     AAELAENLRYVAMDDAELTEVISKTQPADIDAQVASSWEVSKAKSDVTELEGERDSVAR
                     RITSWEEGHGFRTWLNSKGIGGAELDELRTELEQLGNRITKAIRRVRKATEAASARLWP
                     EYNRRITLFDELIELRKAKRQRKILQVQKAQSLDFTTIALQRSLGAFGWADGGREWNAC
                     PKPLQRLIEDYLSTSGGERETMSAEIGRSRDVRDWLAQQKRGIDPAPAPKRNHGPRLG"
                     /name="mobQ"
     gene            complement(3479..4135)
                     /locus_tag="OCX46_RS22085"
                     /old_locus_tag="KAM471c_42500"
     CDS             complement(3479..4135)
                     /locus_tag="OCX46_RS22085"
                     /old_locus_tag="KAM471c_42500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS2"
                     /protein_id="WP_012537714.1"
                     /translation="METYRHTYRHHSFSHQDLSDITFTACTFIRCDFRRANLRDATFIN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIELRECDLKGANFSRANF
                     ANQVSNRMYFCSAFITGCNLSYANMERVCLEKCELFENRWIGTHLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIASWQQEQLLEALGIVVFPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS2"
     gene            complement(4634..4906)
                     /locus_tag="OCX46_RS22090"
                     /old_locus_tag="KAM471c_42510"
     CDS             complement(4634..4906)
                     /locus_tag="OCX46_RS22090"
                     /old_locus_tag="KAM471c_42510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139743096.1"
                     /translation="MSNENHQLFTTEQIRQRLEEGGESARWLAQAVVSYYRPAAPAPSL
                     FSFSTKLDEEAQGMFFQMLLIRSKPDWSDDQLAALVRECEQYLAN"
                     /name="hypothetical protein"
     gene            complement(4927..5310)
                     /locus_tag="OCX46_RS22095"
                     /old_locus_tag="KAM471c_42520"
     CDS             complement(4927..5310)
                     /locus_tag="OCX46_RS22095"
                     /old_locus_tag="KAM471c_42520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139743097.1"
                     /translation="MGGRSVEAFFISCDDHYLAKNLSSIRDEVMAEGEALTNYVRGHII
                     QRRKWGEFDKERARELWGDAEGIEITNSYCSNPGLMTAVVGDEWWYDLPMVDNPDYTYL
                     CRIIQAVRDGLREYTKSTPAAAA"
                     /name="hypothetical protein"
     gene            complement(5663..5911)
                     /locus_tag="OCX46_RS22100"
                     /old_locus_tag="KAM471c_42530"
     CDS             complement(5663..5911)
                     /locus_tag="OCX46_RS22100"
                     /old_locus_tag="KAM471c_42530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182939827.1"
                     /translation="MITPTTETAKEVLRGYGRANEAALASLMDVQDWQRIARQELERRA
                     TSIVQALDDATLKAIAAGELDMRAMCLQVTNEIHQTA"
                     /name="hypothetical protein"
     gene            complement(5929..6882)
                     /locus_tag="OCX46_RS22105"
                     /old_locus_tag="KAM471c_42540"
     CDS             complement(5929..6882)
                     /locus_tag="OCX46_RS22105"
                     /old_locus_tag="KAM471c_42540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000287 - magnesium ion binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="WP_167557713.1"
                     /translation="MNNQNDYIEAAQQIIISLGLPRAQQNERSALCLLALLNLTPGKAW
                     ADAENPLVGITPIMDWAREHYGKEYAPNTRETFRRQSMHQFCDAGVALYNPDKPDRPVN
                     SPKAVYQIEPAALALLRTFGTPAWHDSLTTYLAERETLVARYAKEREQNRIPVEIAPGQ
                     QITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGDKWGYFDAALLTGLGVDVDSHG
                     KMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELAKLFAGSTAGLVYVTAFPNRAIMG
                     RYLGEIAWETEVWVADAPSHLIHFNGVRFLGPYPTE"
                     /name="BsuBI/PstI family type II restriction endonuclease"
     gene            complement(6879..8030)
                     /locus_tag="OCX46_RS22110"
                     /old_locus_tag="KAM471c_42550"
     CDS             complement(6879..8030)
                     /locus_tag="OCX46_RS22110"
                     /old_locus_tag="KAM471c_42550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]; GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="WP_261921576.1"
                     /translation="MTPRILAGDYIELATGEGLLDRGYTHVILNPPYKKINSQSAHRLA
                     LRTVGIETVNLYSAFVALAVGETAPGGQIVAIIPRSFCNGPYYRPFRDFILERAAIRHM
                     HLFESRNKAFRDDEVLQENIIIRLERGGQQGPVTVTTSTDDSFSDLTTHEHPFDRIVFP
                     DDQERFIHVPTTTDKSAIELSPAVRCSLADIGVKVSTGPVVDFRLKAHLRDMPEPGTVP
                     LIYPGHLSMTGTVWPVAGLKKPNAIMRNDETEKWLYPNGFYCLVRRFSSKEEKRRVVAS
                     LVEPTAFGDHSVLGFENHMNLFHEGKHGLPEALARGLVVFLNTTAVDEHFRRFNGHTQV
                     NATDLKQMKYPSRDTLIDLGKWAMQQGVLTQEEIDGKLGTLTA"
                     /name="Eco57I restriction-modification methylase
                     domain-containing protein"
     ARG             complement(3483..4135)
                     /name="qnrS2"
                     /gene="qnrS2"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS2"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..825
                     /name="rep_cluster_1069"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.024"
                     /coverage="82.0"
                     /evalue="1.94e-164"
ORIGIN
        1 atggtaaaac aagcgattca agccgacgaa ttcgtcgaac ggctccccct ccgcccctac
       61 tgcaccgacg atccggcgca gggtctgcat atccgcccgc aggcaactgc gctggccttc
      121 cgtcacattc agcacaaccc accgccgcat gtctcctgca ttgtgtttga tgtggacaga
      181 aagccctatg agcagcggcg agaagggtat caagagtggc gagagcgtgg cttgcctgcg
      241 ccgcattgga tcgcgatcaa ccccgaaaac ggcaattacc acctcggcta tctgctggcc
      301 gcgccagtgg cgcgtaccaa tgcagccagg ttaaagccac tgcgctatct ggcggctata
      361 gagcacgttc tggcgaagaa gctgggcgct gacatggggt atgttgggtt gatcaccaaa
      421 aacccgtaca cagcgattgg tggacgatat ggcataacca tgagccctat tccctcgact
      481 atctcgcgga gttctgccca gatgctgatt tagcggccta caaccgccgt agcggcaaag
      541 aggcgagcgg gctggggcgt aacgtcacgg tgttcgataa cgtgcgggaa tggggctaca
      601 gcgcggtgcg ggagtattgg cggcccaatg gctatgaagc atgggccaat gcggtgtccg
      661 tgctgcttgt gagagcgcta atgcgtttgg acgcgagcag ggcggcccgt tgccccatag
      721 tgagatcaag gccacggcca agagcatcgc ccgttgggta tggaaccgat tcacccccgc
      781 agggtttagc caggtgcagg cgcatcgagg ggctaaaggc gggcgaatat ccaaacgtcc
      841 aactaagaac ggcaaggctc gggcggactt gttgcccgag gtgttagaac tcaaggcgca
      901 gggctacagt aaccgcgaca ttgccgagga tttagggatc ggttcggcaa gtgttagccg
      961 ctatctctcc aaaatctcgc cagagtgatc caaaacaagc ctatatcaga taacagcgcc
     1021 cgaagggcgc ttcttgtctc tagagaatca ttcaagagag aggatcttgg cgaggtgggt
     1081 aactgcgccc gtccgtccat caggggcttg ccccctgtgg gttttttcga gattttcaat
     1141 tcgttgctca atgaccgtca tgctgtttac tcccctcttg tttacccata gcttaccagc
     1201 aataaattag ccaaaaaatg ccgcctaggt gttaatcttt aacccgtaag ggcgcactta
     1261 ggcaaactcc gtttgcgtgc ggcctccggc ggtaaaacat caagacaaga ggcgctgcgt
     1321 ggctatctat cactgctctg ttaaaacatt cagccgacgc aatggtcagt catccactgc
     1381 tgcggctgcc taccgctctg gcgaaaaact acttgatgaa aataccggtg agctgcacga
     1441 ctacaggagg aaaggtgggg tcatttcagc gaccataatc ctaccaagcc atgcccctgc
     1501 atgggcctcc gacagggcgc gtctttggaa tgaagccgag cgcgcagaga cccgcatcaa
     1561 ctcgacggtg gcgcgtgaat ttgagatcgc tctaccccac gaactttccc ccaatgaccg
     1621 cgaacgactg gcccacgaat ttgctcgtga gctggtcgag aaacatggct ttgcagccga
     1681 tgtggcgatt catgccccac cggaattcca gaagggcggc actgggaagg tgatcgacaa
     1741 tcaaaatcat cacgctcata tcctgctcag tacgcgccga ctggggccga acgggttcac
     1801 cgaaaaaacc cgagagtttg atgcgcgatc caatgaacgt gatgacgact cctacctgtt
     1861 cgggccagcc cttattgagc actggcgtga gcgtttcgct cttctccaaa acctgtattt
     1921 agctcgtgca ggcgtggccg ctcaggttga tcaccggagc aacaaggccc gtggcctcga
     1981 tgcggtgcct acccgccatc taggccctgc tgtggttggt ttcgagcggc gtatggggat
     2041 cgacagcgag aagcgccagc gcgaagcgtt ggcggtcagg gcggttgatg agcgtgtgca
     2101 ggagttggat gcagaggtgc ggggggagtt ggccgagctg gatgcacagg aagccgtgat
     2161 cgccgccgaa ctggcggaga atctacgcta tgtcgcgatg gatgatgcgg agttgacaga
     2221 ggtgatctcg aagacccaac cagcggacat tgatgcccag gtggcgtctt cctgggaggt
     2281 tagcaaggcg aaaagtgacg tcaccgagtt ggagggggaa cgcgatagcg tggcaaggcg
     2341 gatcacgtcg tgggaggaag gccacggctt cagaacatgg ctcaacagca agggcattgg
     2401 cggggccgag ttggacgagt tgcgcactga gcttgaacaa cttggcaacc ggatcacgaa
     2461 ggcgattaga cgggtcagga aagcgacgga agctgcgtct gctcgcctct ggccggaata
     2521 caacaggcgg ataaccctat tcgacgagct gatcgagctg cgtaaggcga agcggcagcg
     2581 caagattctt caggttcaga aggcgcagtc gttggatttc accactatcg ccctgcaacg
     2641 ctctttgggc gcgttcgggt gggcggacgg gggaagggag tggaatgcct gccctaagcc
     2701 gcttcagagg ctcatagagg actacctttc aacgtctggg ggggagaggg agaccatgag
     2761 cgcggaaatt ggcaggagca gggatgtgcg cgattggctg gctcagcaga agaggggcat
     2821 agaccctgct cctgcaccga aacgcaacca tggcccaaga ctggggtaag ccgttacagc
     2881 taaaggctac aagtagatga ggagcggcca aatcaaaacg ccaattcgaa atgtcaaaat
     2941 cgcaaattga ttttgaggtg cggggagggg gtgaaaaact ggggccggtg gccccatttt
     3001 cccgccatcg aggcggctta tctgatcggg tgcacctcgt gtgggatgta gatggacaga
     3061 tgccgccttt taggcggcat cttgttcaca ttcacgagca aagtatcatc cttgtgtcgg
     3121 ggtgatgggg tctctggcca gatcctgatg aaaatagagt ttcatcctct ctcaaccttc
     3181 gtgtaggaga ggaataaagt ttcatactaa gcagtcaagt aaccctgttt gtaccgttct
     3241 aaatgatact cacccagaaa taaagattca tactaattcc tactacaaaa tctgagtcac
     3301 gctacaactg agtaataaag tatcatactg gttgtcttga gcatccacag attgattggc
     3361 cccattcaaa cacgtttctg gacaagaatg agctagaagt attgatctag ctctggtgat
     3421 ttgaattaga aaatgtatta atgttgtgta tagtttatca gcaggtattc gtaagcatct
     3481 agtcaggaaa aacaacaata cccaacgctt cgagaagctg ttcttgttgc cagctggcaa
     3541 ttttgatacc tgatgtatcg acttttcgag gatctaaacc gtcgagttcg gcgtgacata
     3601 aattagcacc ctgtaggcta aactgtcccc agacatcttc agaaaaaaca cctcgactta
     3661 agtctgactc tttcagtgat gcgcccgcga ggtgagtccc tatccagcga ttttcaaaca
     3721 gctcacattt ttctaaacag acccgctcca tattggcata agacaggtta catccagtaa
     3781 taaaggctga gcaaaagtac atacgattac tcacttgatt ggcaaagttt gctcgggaaa
     3841 agttggcccc ttttaaatca cactcacgta actcaatacc gtagcaattg gcgttactaa
     3901 agtttgccat cgcaagctgg cattgttgga aacttgcatc gcgaaggtct gcgacatcaa
     3961 aatggcaacc ttcgatatca ccctgttcaa tgaacttgca gttaataaat gtcgcatcac
     4021 gcaagttagc acgtcgaaaa tcgcatcgga taaaggtgca agcagtgaaa gtaatatcac
     4081 ttagatcttg atgtgaaaaa ctgtggtgtc gatatgtgtg acggtaggtt tccatgttga
     4141 ctcctatgat gaactggacg caacactacc atcaaagcca catcacatca ccaaaataaa
     4201 aacactaaaa atcaagatct taaaaaccct cattttgcct attttaaggg ggtaaaatag
     4261 cgccctgagc ctctatgtta acgttcaaag tacgcggcac cattgaaaaa agttatccgc
     4321 ttaaaaagcc acagagaaaa ggaacccaat agattactga tgtccgccat taggtaaagt
     4381 aaccttaaac ggaagcctgc aatttgaagt agatacaagg tctaagaagg aatggggcat
     4441 aaacgtttta gaaatggcag tgtactctga tcgctgccat ttaagtatga atctttaata
     4501 ctcagtatga ttctttatta cttagtaacg aaactttatt ctactcacac acttcgatca
     4561 ccacagccgc agcataaaga acccctttct gcaactctgt aagctttgcc ttagtcatgg
     4621 cttttttcct ctatcagttg gcgaggtact gctcgcactc gcgcaccagg gcggccagct
     4681 ggtcatcaga ccaatccggc ttgctgcgga tcaacagcat ttgaaaaaac attccctgcg
     4741 cctcttcgtc cagcttcgta gagaaggaaa acaggctagg ggctggtgct gcaggccggt
     4801 agtaggagac aacagcctga gccaaccagc gggcagattc gcccccttcc tcaaggcgct
     4861 ggcggatctg ctcggtggtg aatagctgat gattttcgtt actcatgagt ccgtttttcc
     4921 tctaactcag gccgctgcgg ccggggtgct cttggtgtac tcgcgcagac catcacggac
     4981 ggcctgaatg atgcggcaca ggtaggtgta atcagggtta tcgaccatcg gcaagtcgta
     5041 ccaccactca tcccctacga cagcagtcat cagccctgga ttactgcaat atgaattggt
     5101 gatctcaatc ccctccgcat caccccacaa ctcgcgggcc cgctctttat caaactcacc
     5161 ccactttcga cgctggatga tatggcccct gacatagttg gtcagcgcct ccccctcggc
     5221 cattacttca tcccggatcg aactcaggtt cttggccagg taatggtcat cacaggagat
     5281 aaagaacgcc tccacactgc ggccgcccat tgcgccccat ggttagggtg agactttatt
     5341 ttcaaggatg aaactctatt ttcggggatg acactttatt ttcatcccac acctcgacgg
     5401 gcggagcaag atatcgaaac aggccgcagg atgcaaaaac aggccgaaac cataccccgc
     5461 aggatctggc cctcaaaccg cctcagaagc gttgcaaact ttcaggcagt aaatgccctg
     5521 tcttgtgtgt cgaagcgccc caaaacgcga tacaggcggc ctgtttgccg ccagcgcccc
     5581 gttttagccg ctaaaattcg cggtttaaca taaaaggcgt tatgcgcacc tcagaacacc
     5641 cttcgattcg ccagttttgg gcttaggcgg tctgatggat ctcgttggta acctgtaggc
     5701 acatagcccg catgtctagc tctcccgctg cgatggcctt aagggtcgca tcatccagag
     5761 cctgcacgat gctggtagcc cggcgctcaa gctcctgccg ggcgatgcgc tgccagtctt
     5821 gcacgtccat cagggacgcc agcgcggcct cgttggcgcg tccgtagccg cgcagcacct
     5881 cttttgctgt ctcggtggtg ggtgtaatca tggtctggcc tcctgtgctt attcggtggg
     5941 gtaggggcca aggaaccgaa ccccgttgaa gtggatcagg tgagaggggg catcggctac
     6001 ccatacttcg gtttcccatg cgatttcccc gaggtagcgg cccatgatgg cccggttcgg
     6061 gaaggcggtc acatagacaa gcccggcagt cgatccggca aacagcttgg caagctcggc
     6121 gtgccgcttt ccatcgaccg gcccgtgact ggtgacggac tccaccagta gcagccagtt
     6181 tttcgcggtg aagtgcagca ccacgtcagg catcttgccg tgtgaatcca catcgacacc
     6241 cagcccggtc agcagggctg cgtcgaagta gccccatttg tcgcccgtgt cgccggcata
     6301 gaccagcacg ctacccggcg caaagcgcgg tgcgaagtcc tcgatgatgg cccggattag
     6361 ttcgctatgc tcgccggggc tgagggtgat ttgctggccc ggtgcaatct caaccggaat
     6421 gcggttctgc tcgcgctcct tggcgtagcg ggcaaccagc gtttcacgct cggccagata
     6481 ggtggtgagg ctgtcatgcc atgcgggggt gccgaaggta cgcagcaggg ccagcgcggc
     6541 gggttcgatc tgataaaccg ccttggggct attcaccggg cggtcgggct tgtccgggtt
     6601 gtagagggct acgccagcgt cgcagaactg gtgcatggac tgacggcgga atgtctcgcg
     6661 ggtgttgggc gcgtactcct tgccgtagtg ctcccgtgcc caatccatga tgggcgtgat
     6721 gcccacaagc gggttttccg cgtcggccca tgccttgccc ggcgtgaggt tcaggagggc
     6781 cagcaggcac agggcagaac gctcgttctg ctgcgctcgg ggcaggccca aagaaatgat
     6841 gatttgctgt gcggcctcaa tgtagtcgtt ttggttgttc atgcggtcag ggttcctagc
     6901 ttgccgtcga tctcttcttg cgtgagaact ccttgctgca tggcccactt gccaagatct
     6961 atcagagtgt cacggctcgg gtacttcatc tgctttaggt cggttgcatt gacctgcgta
     7021 tggccgttga atcgccggaa atgttcatct actgcggttg tgttcaggaa cacgactagt
     7081 ccacgtgcca gcgcctctgg caagccgtgc ttgccctcgt gaaacaggtt catgtggttc
     7141 tcaaaaccca acacggaatg atcgccaaag gcggtaggtt caaccaagct tgccaccacg
     7201 cggcgctttt cctcttttga ggaaaatcgg cgcactaggc aataaaaccc attcgggtaa
     7261 agccactttt ccgtctcgtc gttgcgcatg atcgcgttgg gcttcttcaa gcccgccacg
     7321 ggccagacgg tgccggtcat gctcaggtgg ccggggtaga tcaggggcac ggtgcccggc
     7381 tctggcatgt cgcgcaggtg cgctttcagc cggaaatcga ctaccggccc ggtcgatacc
     7441 ttcacgccaa tgtcggccag cgagcagcgc acggcgggcg atagctcgat ggcgctttta
     7501 tcggtggtgg tcggcacatg aatgaaccgt tcctggtcat ccgggaacac gatccggtcg
     7561 aacgggtgtt cgtgggtggt caggtctgaa aagctgtcat ctgtcgaagt cgtgaccgtg
     7621 acaggcccct gttggccgcc acgctccaag cggatgatga tgttttcctg cagcacctcg
     7681 tcatccctga aagccttgtt gcgtgactcg aacaaatgca tgtggcggat cgccgcccgc
     7741 tcaaggatga agtcacggaa cgggcggtaa tacggcccgt tgcagaagct gcgcgggatt
     7801 atggccacga tctgcccgcc cggtgctgtc tcgcccacgg ccagcgcgac aaaggcggaa
     7861 tacagattca ctgtctcgat accgacagtg cgcagagcca gccggtgggc tgactggctg
     7921 ttgatcttct tgtaaggtgg attgaggatc acatgggtat agcctcgatc cagcagacct
     7981 tctccggtcg ccagctcgat gtaatcgccc gcgaggatgc ggggcgtcac gcggtattgt
     8041 atccgtccaa tcgctggagc aactttaagc ctcatgtatt tgcgagtgtt aaccggtgcg
     8101 ggataagttg tttgatgctg taagtttgcg gtaacttatg gcccagaaaa gtaccccgaa
     8161 tctctgttca tcgacttggc ggaagagcac agagagttcg gggccgttca cgaagaacag
     8221 agaaagcata tcacatgact gacctcacac aagactgcac cgccaataag cgcggtgacg
     8281 g
//
