LOCUS       NZ_AP026084             6675 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Escherichia coli strain F765 plasmid pF765_2, complete sequence.
ACCESSION   NZ_AP026084
VERSION     NZ_AP026084.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00495867
            Sequence Read Archive: DRR381603, DRR381752
            Assembly: GCF_025995255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Miyata,T., Taniguchi,I., Nakamura,K., Gotoh,Y., Yoshimura,D.,
            Itoh,T., Hirai,S., Yokoyama,E., Ohnishi,M., Iyoda,S., Ogura,Y. and
            Hayashi,T.
  TITLE     Alteration of a Shiga toxin-encoding phage associated with a change
            in toxin production level and disease severity in Escherichia coli
  JOURNAL   Microb Genom 9 (2) (2023)
   PUBMED   36821793
  REMARK    DOI:10.1099/mgen.0.000935
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Hayashi,T. and Miyata,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2022) Contact:Tatsuya Miyata Kyushu University
            Graduate school of medical sciences, Department of Bacteriology;
            Maidashi 3-1-1, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3
            Genome Coverage                   :: 67x
            Sequencing Technology             :: MinION; Illumina Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025995255.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:48:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,449
            Genes (coding)                    :: 5,138
            CDSs (with protein)               :: 5,138
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 121 of 311
            Pseudo Genes (incomplete)         :: 179 of 311
            Pseudo Genes (internal stop)      :: 67 of 311
            Pseudo Genes (multiple problems)  :: 45 of 311
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026084.1.
            This clone was obtained at our laboratory.
            Please visit our website
            http://www.bact.med.kyushu-u.ac.jp
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F765"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF765_2"
                     /geo_loc_name="Japan"
     gene            complement(join(5494..6675,1..912))
                     /gene="cda"
                     /locus_tag="OO934_RS27775"
     CDS             complement(join(5494..6675,1..912))
                     /gene="cda"
                     /locus_tag="OO934_RS27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            1176..1421
                     /locus_tag="OO934_RS27780"
                     /old_locus_tag="CL8F765_53240"
     CDS             1176..1421
                     /locus_tag="OO934_RS27780"
                     /old_locus_tag="CL8F765_53240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            complement(1911..2144)
                     /locus_tag="OO934_RS27785"
                     /old_locus_tag="CL8F765_53260"
     CDS             complement(1911..2144)
                     /locus_tag="OO934_RS27785"
                     /old_locus_tag="CL8F765_53260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137424.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVLRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2151..2711)
                     /locus_tag="OO934_RS27790"
                     /old_locus_tag="CL8F765_53270"
     CDS             complement(2151..2711)
                     /locus_tag="OO934_RS27790"
                     /old_locus_tag="CL8F765_53270"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001345151.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2746..3426)
                     /locus_tag="OO934_RS28045"
                     /pseudo=""
     CDS             complement(<2746..3426)
                     /locus_tag="OO934_RS28045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3389..3712)
                     /locus_tag="OO934_RS27800"
                     /old_locus_tag="CL8F765_53280"
     CDS             complement(3389..3712)
                     /locus_tag="OO934_RS27800"
                     /old_locus_tag="CL8F765_53280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3780..3971
                     /locus_tag="OO934_RS27805"
                     /old_locus_tag="CL8F765_53290"
     CDS             3780..3971
                     /locus_tag="OO934_RS27805"
                     /old_locus_tag="CL8F765_53290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4123..4311)
                     /locus_tag="OO934_RS27810"
                     /old_locus_tag="CL8F765_53300"
     CDS             complement(4123..4311)
                     /locus_tag="OO934_RS27810"
                     /old_locus_tag="CL8F765_53300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177148.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     gene            complement(5018..5164)
                     /locus_tag="OO934_RS27815"
                     /old_locus_tag="CL8F765_53320"
     CDS             complement(5018..5164)
                     /locus_tag="OO934_RS27815"
                     /old_locus_tag="CL8F765_53320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            complement(5234..5497)
                     /locus_tag="OO934_RS27820"
                     /old_locus_tag="CL8F765_53330"
     CDS             complement(5234..5497)
                     /locus_tag="OO934_RS27820"
                     /old_locus_tag="CL8F765_53330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     BGC             1..5912
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4057..4140)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="1.15e-36"
     Typing          complement(4602..4789)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="100.0"
                     /evalue="2.18e-82"
     Typing          complement(1805..3399)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3772..3974)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.85e-12"
ORIGIN
        1 tgctttattt tcggcttcct gacgctgact cagaccatta ctgtccagaa tctctacagt
       61 tgaaatatat aacggagaaa caccactgcc atcaggaaaa cgaacaatga catcatgatt
      121 attactggac atcgtataac cagcctcaac ataattacct gtctcagcaa taactccttc
      181 ttcctgaatt tcgggattag acagaaccgg agtattcatc tgacccacac ttaataccag
      241 gtccggtaga tttggtgata caggggcact gtaaacatct ttaatatttg ttcccggacg
      301 actccggtcc ggaactggaa cggcatcaac taccggcact gtcatcggaa tacctccgat
      361 aatagccata tattgcgtac cgttctgcat aaggtccatc agacgatatt tcaccggtac
      421 attattaccc gctgctggta actggctggc cggaatatcc gttacctgtt ccagttgctg
      481 tgtaaccata agacgaataa ttttttgctc atttccggaa gcaggctcca gacgggcaaa
      541 agaaacagca gccgggcgag acggttctgg caaaccacca cgtaaaccat tctcaagaac
      601 tgttcgtgca gcggaaactt tctcgtttcc ccaagggcta tgcacactga cagaccatcc
      661 cgaccagtct ccctcttcaa gttccttatc cagaataatc cggcgaataa ttcccggaaa
      721 aagaacactt tgtacaagtg cgataccctg tgggctgtaa tccccccatc ccggaggcat
      781 ggatggagcg atacctgatg agcctcctgg tttcacatca ccacccgaaa tcagtcctgt
      841 tgacggccac acgaccatcg agtgcccata atcaatacct tctgtcggac cactaccttc
      901 gtaatcactc ataaaaacac ctctttgact tttaaaacaa tgcgttaaaa acaacaaact
      961 gtacataaaa ccactggttt tatgtacagt aaaaagttac gtcacctcgt aaatcctgtc
     1021 aacccactgg atttacgttt ttgtgaaaac gctcaagaaa aacaaccaat cattcttaaa
     1081 ctcaatggca acaggcatga aagaaggctg attagcataa ggagagagcc aacagttaaa
     1141 ctgttatgac accagagaaa cacccggaga ccaccatgaa acgccataga ctgaccgaag
     1201 ccgaagagcg ccgactggca gaagccattg ccgctgaagc tcgtgccatg gggctgacga
     1261 aaaaggacaa aatgccgcag gaaacaaagc gacgcctccg ggcacggata cttaaagcca
     1321 ttcttgatga acgaacggcc agcaaaaatc gagaagccac actgactgcc ggacaggacc
     1381 cggatgacac cttcacctgg caaccactgc gccgccgcta agctaagggt tatttaacat
     1441 aaccccttat tgtacgcacc gctgaaatgc gttcagcgcg atcacggctg ctgacaggta
     1501 aaaatggcaa caaaccaccc gaaaagttgc cgcgatcgca cctgataaat tttaaccgta
     1561 tgcatagcta ttcagccatg tgaataacgc tggttttgcc tgcgtaaacc tcatgacact
     1621 gttttttttc catcttttca gttgatgaca tacgcagaca tcgcgggatg aggctgagga
     1681 atgagcgcga tctggcaaag aggcaaaaca cagcaacaaa aacgacacgc cagaatcgcg
     1741 cccggatgcg tttttaacgc gttccggtac catctggcaa cctcccggaa caactcaccg
     1801 tcacatcccc attgacgggc cacgccatac ccgtgcttcc cgttcctgct cttcatgcca
     1861 ggaccgcgca cgctcccgtt ccaggcgtgc ctgcctttcc tgttcatccc ttatctgctg
     1921 ttcgtgataa ataaccgact caagcggtcc acctgcccgg ctaatctcag cacctgctga
     1981 ctcaagtcgt cgcactgttc cctcagttgc ccgttctcct gtcgtgtcag ctcgaacata
     2041 tgctgcaaat ccgtgaaggc gctctcccag tctttcagcc gctgcgtata gtcctgctgc
     2101 aattgctcta aggcgttcag taagtgcatt tccagctctg tcatactcac ttactccctg
     2161 accagtctta ctgcgttctt cttctccacc gtccagttgt tttccccctt caccccggac
     2221 ggcaacacca gaaatttccc gttcctgccc tcgtgatacg tcacacccca tgttttttcc
     2281 cggagtttcg ccagcgtctc ttcctggtcc ctgatagcca ggatgttcgc cgcaatacgg
     2341 ctttcctgcc actgaatcag cccccatgac gccagaaaaa tcccgaccgt gacgcaaagc
     2401 gccgtcagcg acgggtacag tatccgccct ttgaccagct tccagagcag ctcttcctgc
     2461 cgccgggcaa gttcgttctc tgtggcgctg aactgcgcgt tcacggcact gttcagcgtc
     2521 tccagttgtt ctttcaccgc tgctgtgtgt gcgctgatag cgtctctgat tttctgcccg
     2581 tttaagttca gttccctgtc tacagacgct tcgagcttcc tgaactcgct gttcagcatg
     2641 ttctctgtag agacggcacg ctctttcagt ttcttctcga agtctgtccc catttgtaaa
     2701 agattgctca tacagcgccc ctttcagcct gagattacgc ccgccctccg ggtcggcgat
     2761 actgatactg ctcctggttg tcctcaccac ctcaaaacct gccgctgtaa gcgcctcagt
     2821 gacatcctga cgcgttttta gcgctccggc atggtaaaga gcctccagtc ccctcgtaat
     2881 cgcttctgcg gcctcctgtt tcgctttcgg cagattattc ggggtgacaa gtgtccgcct
     2941 gttctccggt gcgttcgggt cgtgcagccc gtaatggtga ttcaccagtg tctgccaggc
     3001 attgattcgc ggacggtccg cccggtcgta atagggctgg agccgttttc cgctcgccag
     3061 ctccatattc gggatgacaa aattcagctc aagacgcccc ttgtcctggt gctccaccca
     3121 caggatgctg tactgatttt tttcaagacc gggcatcagt acccgctcaa agctctccat
     3181 caccctttca cgctctcccg gtggcagtgt ctgctctgca aaagacagaa cccccgaggt
     3241 gtattttttc gcaaacggcg tggcatcgat gagttcccgc acctcttcgg gagcaccccg
     3301 cagaactctc gccccttccc ggttacgctc ccggcccagc aggtaatcaa ccggaccact
     3361 gccaccgcct tttcccctgg catgaaactt aactatcatc gcgttctccc tgtttacgga
     3421 cctcatccct cagctcactc agttcacgtc cgatggccat cagtgcagcc accacatgaa
     3481 cccggtcatg ccccgaccac tgtccactgt ttatcttccg ggctatctga ttcaggttat
     3541 tgccgaccga agcgaactgg cgcaacagcg gcggtgccag tgtcggaaga cctgacgttt
     3601 tcgatggcgg tgcccccagg cagaccttac gcatccatga cgcaagttgt tttccctcac
     3661 aacgtgccag cagccgcgca tgttcctcat ccgtgacccg tatcgtgagc atcctttcgc
     3721 gtttcaccgg tatcattaaa aacctccgac agactcccca cacatggaga aacagaactg
     3781 tgactaaaca ggaaaaaacc gcccttaaca tggcccgctt catcagaagc cagacgctga
     3841 ccctgctgga aaaactgaat gaactggacg ccgacgacca ggctgacatc tgcgaagcgc
     3901 ttcacgacca cgctgacgag ctttaccgca gctgcctcgc acgcttcggg gatgacggtg
     3961 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgcctgtga gcggatgccg
     4021 ggagcagaca agcccgtcag ggcgcgtcag cgggttttag cgggtgtcgg ggcgcagcca
     4081 tgacccagtc acgtagcgat agcggagtgt atactggctt aatcatgtgg catcagtgag
     4141 gatggtatga aaagtgcacc atgccgggtg tgaaatgccg cacagatgcg taaggagaaa
     4201 atgctcgtcc aggcgctttt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     4261 gctgcggcga gcggtatctg cccactcaaa agcggtgata gtgttatcca cagaatcagg
     4321 ggataacgcc ggaaagaaca tgtgagcaag aaacgaaaac cccgaaaaag gccgcgccgg
     4381 aggcgctttt tccataggct ccgcccccct gacggacttc acaaaacaga cactttttcg
     4441 gtgcatctgt gggagccgtg aggctcaacc atgaaatccg ccagcggtgg cgaaacccga
     4501 cgtggcttaa agatcatcac cgtttccggt gaatcgctcc ctcctgcgcc cccacgttcc
     4561 cgccatatcg attaccggat accttttccg cctttctccc ttgcgggaca cgtggcgctt
     4621 tctcatagct cacgtcttct ggtatctcgg ggcgacttag gcagttcgct ccgggctggg
     4681 ctgtatgcag gaaccccccg ttcagcccga ctgctgcacc ttctccggta actgttctct
     4741 tggggccaac ccggaaaaga cacgacttaa cgccactggc agcagccatt ggtaactgat
     4801 ttagaggagt tgctcttgaa gttatgaacc agttaaggct aaactgaaaa gacagttttg
     4861 gtgactgtgc tcctccaagc cagttaccac cgccgattag aagaggtcga agacttctga
     4921 tcggacggaa ggacccgcca caaggcgggt tttttcgttt acaggcagca gattacgcgc
     4981 agaaaaaaag gatctcaaga agatccttta atatttttca ctgaaccgcc accccgatta
     5041 gtttcgaaga tgatgatgga gcaattgttc caccctgaac atcccttaca tagttagctt
     5101 ggcaagcagt cagacacaaa acagcaagaa cgagaatcat agatccccct atcttttttt
     5161 tcatgacatc tccttgtcat gagtgaatat ctgacattta tacttaaatg ccagatatta
     5221 aaatcactca tgattataat ttaaattttt ccaagataga tcggacctca accttaagtc
     5281 cattatcatc aatttcataa tcagccctat ctgcatcagg ctcaaatcgc tcagcagcca
     5341 taaacaactc ttccccacaa tttaatatat tcggagacaa atccttaaca ccatacaatc
     5401 ttctccgttc aacacaaatt cgctctgaaa actcaattgc tgtaattttc gaagctaaaa
     5461 ataatttcgc caaatcgatc attgccatct tgttcatagt tcacccgtct ctaaataaat
     5521 tcgaccatta tccgcatctg gatttatttt ccacccagat aagaactcac catttgactt
     5581 aattataacc acattcatcg tattaggatt aaaataaact tttgaacctt tttctcttcg
     5641 gtatgttcct ttctctactg tatccttatc cgataaatgc tcctcaatag catccctgaa
     5701 tttagtaaga gtttcacgat tcttttttgt atcactaata ccaaaatcac cagcatgttt
     5761 atatttttta tcaagttgtt tacgcgaaaa tcgccctgaa tccagaggat cattaagcat
     5821 cacataaaca gcttcaacac cagtcccagc agcatcagga cgccagtaaa taaaatctcg
     5881 tagtccacca acatcagaaa caggagtaat cgtcgttact ggcatttcga ccggttttac
     5941 ttccgttcct gtatgcactg gtgtaaccgg aacgggttga tgattacctg tatctgttgg
     6001 caccgaaggt tgtggtaccg gattaaccag aatcgtccgg gaaggtgcgc ctactactgc
     6061 tggtaacgtg attttatcca gtcctgttgc tgtatcacgg acagcattaa gaacaggaac
     6121 cgcagctggg atactttcat tccctgtttt cagtaaatcc agagctaatt gcccgttact
     6181 gtttatcaat tgtccacgaa caggcagatt tacactcgtc gcaccaggtt caatgaccac
     6241 gccctgtcca gccaacatct gagcactcag cgaaaacatc gcctccagat ttcgccccgg
     6301 cacgatatca cttcctgctc cagcttcttc tgaatgaaac cacagagcac caagagaaat
     6361 ccgcataagc aggggactca cttcgggtat ggttactttc tccagagcat tactgaaaaa
     6421 cttgcttatg ttgcctgcaa tatcaccagc agcagtccag acaccagttc cggtcatcgc
     6481 cagttgtaca gaactggcag tgttattcag aacaagagct ccctgctcat tcattactga
     6541 attagccctt tccatcattt cctgtgtata tacaggaggc tggttcacac ctgctttact
     6601 aaataactcg gcttttgctt tttcagcttc agcccgggct accgcttctt ctttcttgac
     6661 tctgaaatca tcctt
//
