LOCUS       NZ_AP025935             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80382 plasmid pFJMB80382_4,
            complete sequence.
ACCESSION   NZ_AP025935
VERSION     NZ_AP025935.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490893
            Assembly: GCF_023736015.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 283x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023736015.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:19:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,258
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 5,050
            CDSs (with protein)               :: 5,050
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 25 of 93
            Pseudo Genes (incomplete)         :: 71 of 93
            Pseudo Genes (internal stop)      :: 12 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            Pseudo Genes (short protein)      :: 1 of 93
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025935.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80382"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80382_4"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-08-20"
     gene            809..952
                     /locus_tag="NGK50_RS26235"
                     /old_locus_tag="FJMB80382_51560"
     CDS             809..952
                     /locus_tag="NGK50_RS26235"
                     /old_locus_tag="FJMB80382_51560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1380..1607
                     /locus_tag="NGK50_RS26240"
                     /old_locus_tag="FJMB80382_51570"
     CDS             1380..1607
                     /locus_tag="NGK50_RS26240"
                     /old_locus_tag="FJMB80382_51570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1974..2159
                     /locus_tag="NGK50_RS26245"
                     /old_locus_tag="FJMB80382_51580"
     CDS             1974..2159
                     /locus_tag="NGK50_RS26245"
                     /old_locus_tag="FJMB80382_51580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2207..2286)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          414..974
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gccggttcag tttcagacaa aatctgccgg tatgcatcca gcatcatggc tccggcatcc
       61 ccccggcacg ccggagccag cccgaaacaa cagacacaaa caggtgtcgc tcatcatcaa
      121 gagtcatcag gtctccggaa gaaaaaccaa acatggctta cctcctgtta acggcaacgc
      181 agaagaaagg tttacagggt agcgggcagg aaaataaaaa aattgcagtc agcccggaac
      241 ggctgctgaa ccggtacagg cgggttccgg gcgcagcgcc ggaccagtca ctcagcgcta
      301 gcggagtgta tactggctaa aatgtaacac cgcagtaacg catagcataa ctgctacata
      361 agacaggcgc agaaaatgaa agaaaacgcg tcaggagaaa atgtaataca ggatatattc
      421 cgcttcctcg ctcactcact cgctgcgctc ggtcgttcgg ctgcggcgag cggtacgggc
      481 ttacgggtgg agcagaaatt tcctagggga tgccagctag gggatgccag gaagatactt
      541 agcgaagaag gcataaggcc gcgccgaagc cgtttttcca taggctccgc ccccctgacg
      601 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgatgcgg cttaaagata
      661 ccaggcgttt ccccctgatg gctccctcgt gctcccccgc attcctgcct ttcggtttac
      721 cggtgtcact ccgctgttat ggccgcggtt tctcattcca cgcctgacac tcggttccgg
      781 gaaggcagtt cgctccagga tggactgtat gcacgaaccc cccgttcagt ccgactgctg
      841 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacacgtaa aaacgccact
      901 ggcagcggcc actggtaagg ggtatacaag attactggtg cagagttctt gaagttgacg
      961 cctgactgcg gctacactag aaggacaaat ttggtggcag cggtcttgta caccctgtta
     1021 ccacggttca gaaacttgcc ggtttgctga accttcgaaa aaccaccttg ccgggtggtt
     1081 ttttcgttta cagggcaaga gattacgcgc gaaaaaaaag gatctcaaga agatcctttt
     1141 ataaattaga catccaacct cacaaagagg aatacttaaa tcatcttggt atatgtatcg
     1201 gtcggattac atcagatata agcccgcact gccccctccc cgccagacac cacactttaa
     1261 ccataacacc tcgcacctaa ctcgatatgc agcccaccat caagcctctt ttcacataaa
     1321 aaatgaccta tagttagatg gttagtgacc aaaactgata tggataaaag gagctggctt
     1381 tgaaaaagat ttttgtttcc gtgttcgcag cggcagttgc gctttcagcc ctcaccggct
     1441 gcacgcgcac aagctatgcc atacacacca atgacgggcg cactattgtc agcgacggca
     1501 aacctaaaga atcagactca ggcctgcttg gctatacaga cgcgaatggt gtcaaacagc
     1561 agatcaataa aaccgacgtt aaagaagtct ccgaaattcc gcactgagca tcttctccga
     1621 aaaaggccgc taatgtggcc tttttcttta cctggggttc tccgccaaaa tcccagcaaa
     1681 ttgcaccacc aaagctaaaa gcttccaggg ttgtctaaaa agcacaagac attagaggaa
     1741 gtgcggtttt tataatcaag ccccccaaga atttaccata acatccgttg cccgcaccgc
     1801 ctcagacgca ctcagcgctt ttctgacgga accatgacca aaaaccacaa caaaccaccc
     1861 cggaacccgc cagaaacgct cctgttaaat tttaacggca tgcatgacta tgcaccagaa
     1921 tgacggcatg ctcttttcac gcaaaaatca tcaccagacg gggaaaatca ccagtgacca
     1981 gacaggaatc cgccgccctc aacatggcca aatttatccg ctcacagaca ctcctcctcc
     2041 tcgagcggct cgaacagatg gatctggatg aggctgccgg ctgctgtgag cacctgcacg
     2101 atcaggccga agcactttac accatgctga acgcacagac aggcgaggaa gatgcgtgaa
     2161 aatcggtgaa cgggtgcgca attcagtgcg cggccgtgag gcgatggcgg ggtgtcgggg
     2221 cgcagccctg acctgggtat ttgtgatgcc ggcgcgtgcg cggtattaca aatacacatc
     2281 ctgtcctgag tggttccccg ggaacaggca aaaaaaaacc ggggagctgc ccggaaggta
     2341 agtcaagtta gattagatta ctttcactca tccataacag catcatgcaa cgacgaccat
     2401 cgtccgtgac ggccgcctcg tttaagtatg gacagaaata cagaaaatgc tcaggacgaa
     2461 atgtaatgaa tgcgaacgga ttcaagaaat tcgagcatga ccgtccttac g
//
