LOCUS       NZ_AP025934             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80382 plasmid pFJMB80382_3,
            complete sequence.
ACCESSION   NZ_AP025934
VERSION     NZ_AP025934.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490893
            Assembly: GCF_023736015.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 283x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023736015.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:19:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,258
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 5,050
            CDSs (with protein)               :: 5,050
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 25 of 93
            Pseudo Genes (incomplete)         :: 71 of 93
            Pseudo Genes (internal stop)      :: 12 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            Pseudo Genes (short protein)      :: 1 of 93
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025934.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80382"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80382_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-08-20"
     gene            65..175
                     /locus_tag="NGK50_RS26200"
     CDS             65..175
                     /locus_tag="NGK50_RS26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(430..891)
                     /locus_tag="NGK50_RS26205"
     CDS             complement(430..891)
                     /locus_tag="NGK50_RS26205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(1291..2205)
                     /gene="lpxO"
                     /locus_tag="NGK50_RS26210"
     CDS             complement(1291..2205)
                     /gene="lpxO"
                     /locus_tag="NGK50_RS26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(2476..2691)
                     /locus_tag="NGK50_RS26215"
                     /old_locus_tag="FJMB80382_51530"
     CDS             complement(2476..2691)
                     /locus_tag="NGK50_RS26215"
                     /old_locus_tag="FJMB80382_51530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2695..3180)
                     /locus_tag="NGK50_RS26220"
                     /old_locus_tag="FJMB80382_51540"
     CDS             complement(2695..3180)
                     /locus_tag="NGK50_RS26220"
                     /old_locus_tag="FJMB80382_51540"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3215..3919)
                     /locus_tag="NGK50_RS26320"
                     /pseudo=""
     CDS             complement(<3215..3919)
                     /locus_tag="NGK50_RS26320"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3858..4181)
                     /locus_tag="NGK50_RS26230"
                     /old_locus_tag="FJMB80382_51550"
     CDS             complement(3858..4181)
                     /locus_tag="NGK50_RS26230"
                     /old_locus_tag="FJMB80382_51550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4283..4469)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          2..392
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.415"
                     /coverage="98.0"
                     /evalue="6.65e-97"
     Typing          4449..4754
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.379"
                     /coverage="92.0"
                     /evalue="5.09e-93"
     Typing          complement(2379..3868)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga
       61 ctgtatgcac gacccccccg ttcagtccga ctaccacgcc cattccggta actatcaact
      121 tgagtccaac ccggaaagac acgacaaaat cgccattggc ggtagccact ggtaagcagg
      181 atgtgcgaga gatgtatcga gagttcttga agtggtggcc ggactacggc tacactggaa
      241 ggacagattt ggtgacacgt ctcgcacaag actgttacca cggttcagaa gttccccaac
      301 ttactgaacc ttcgaaaaac cacctcccca ggtggttttt tcgttttcag agcaagagat
      361 tacgcgcaga gcaaaacgat ctcaagaaga tcattttaca aattggataa tatccctccg
      421 cccctaacac taatataaaa ttatcgggct tgcatgcccc tcaccatttt ccccatatcc
      481 accaccaaac aatagttcgt cgagttcttt gctagcttga tcaatacacg cagccacatg
      541 aatagcagaa gggtactcat catactgaaa attatcgctt ttttcaggag ggagtcgatc
      601 ataatacgga tgacaatcca cataataatg acgtattagc atcccagttt tatcttcgta
      661 ctctgatggc tgcaacgtac tccctgggaa agcttttgca acatgtatag cagcttgaaa
      721 ggtatcaaaa tatcgcccaa acattggcgt ttcaaagcta ggcgactctg tcgaaaaatc
      781 ccccataact tgcgccaaat aaccattatc tatctgccat tgaacatctg atcccatagc
      841 ccactctccc agagttactc ctaataaata accataatat gtatttacca taacgcttct
      901 tggccgcacc ccctgagagc ccctcagcgc gtttctgcgg cctgagggcc gtttccggca
      961 cctttccccg gcccaaaccc tgaaaaacgc ccctgataat ttctgcgcgg ctgcatggct
     1021 atgcacaaga gtgaatggcg gccggtttcg tgaaggaaat accgtgaaag ctccgaaaga
     1081 aacgcttaat tgcacagcag cacatgaacg tgatcagatg aaatctgtct actgccgcac
     1141 ttcttcacga aaggcccgct gagcgtcaga ttcgggcctt tcttactaaa aagatttcaa
     1201 tagaattttt tagcaggcga tttttatcta taaccataca ttgagcatag aagacccaaa
     1261 tttatgtatg actcaataga aaaaaacaaa tcacagccat ctaaacactg agacagcaag
     1321 ccaaacaaaa atcaaagaaa taactccatt tttaatcaaa taatatagtt ttcgattcct
     1381 tttttttagc ttctgacctg catcacgaat agcattaaca tatttaaata aatgatttat
     1441 agcaccagtc ctgtcattat tatcgttggg tgatgctgcc gctgacatta gcaccccacc
     1501 tatccaccta tttatttttt gtgaccaccg ccacttcatc ggcctttcta tatcacagaa
     1561 caaaataata cgtgtctgct cggtatcatt ctgagcccag tgaacaaaag tttcgtcaaa
     1621 aattatcact tcaccatcat gccaactgcg gcgtacacca tcaacttcaa taaaacaccg
     1681 ttcatcattt ggtgttgata atcctaaatg ataacgaact gaaccggcat aagggtcacg
     1741 atgctttcct aaatggcttc ctgaaggcaa ttcagcaaac atcgctgctt taatacaggg
     1801 aatagagcta accagttggg ttgttactgg gcaaagccgc tccgctgatg gatgagcatc
     1861 tccataccac ttgaggtaaa agcgcttcca accgcgctta aaaaatgtat taaatcctgc
     1921 atcattattt ttttcagatg atttaatgtg cccttgcaaa ttaacagctt cgtctcttat
     1981 aacctgccag ttatcaatta atggttgaag ttcaggaaat gtttctgggg gaaaccatgc
     2041 ctgctttgac ggcagcttcg aaaagcctgt cataaacata tttatcggag ccataaatgt
     2101 tgaatgatca aataactgcc tggaaaactt ttgttttatt ttaccgcgag aatgcgcata
     2161 caatacactg ataagaaaaa caaaaataat aataagtgac agcatgtgta tattccttgt
     2221 tcacgccaat aataaaaatc tcttaaagat aacataatga atgcacttaa attataatct
     2281 aaatgctatt tatcaatagt ataatcgaag cttcaccaga acatatctac tacacaggca
     2341 gagaggattt cagcaggaat tatgctgaaa tgaatcaaag ggacggaccg tgatataccc
     2401 gctgtcgctg tgatcgttcg agctccagcg ctttctgatg ccgcgcctcc cgcacctgct
     2461 gcagctcgac ggcattcagc cgctcgacaa ccggcgcagc tgatcgctca aactctgcac
     2521 ctgcctgctc aagcccgtga cgcgctcgct cagcgccgcg ttctcccgtt gcataagacc
     2581 agacatcgtc cgccattccg cgaaggcgct ctcccactcg tccagccttt tcgagtagtc
     2641 ctgctgtagc tgctctaatg cgctcagcaa ctgtttttcc agctctgtca tgtgctattt
     2701 ccccgccagt atcatccagc tcgaatcctc tccgaaccgt cccgcttccg ggttcaccct
     2761 cacgcagcgg cgtctctgct cgccgcaggt cgagagctgc acgccgctgt tccgctcgga
     2821 cagcatcgcc tgcgtgctct tctgctcccg gatgctcacg taattgtcga gtatctgctg
     2881 actctgccac cacagaatgg ccgcgttcga ggcgatcagc agcacggaga ccaggacgat
     2941 ggtcagccac gtctggctga ccatgcgcag catccctttc gtacgctggc tcatggctga
     3001 ggacagcttc cggtcgtggt cgaggatggc ggcgctgatt ctcttctcgc tttcgctcag
     3061 ttccgctctg acagacttct cgtgctcgct gaatgcggct ttcagcatct cgccggtact
     3121 ctgctgctgc gctttcgatt tctgctctaa gtccttcgcc agcgttaaaa gactgttcat
     3181 agatggctcc cttcagtcgg atgtttcgcc ccccgtccgg gtcggcaatg ctgatgctgc
     3241 tttttgtggt gcgtaccacc tcaaaacctg cgctttccag cgcctcagtg acgtcctggc
     3301 gggttttcag ctccccggac gaggccaagg ccagtaaacc ccgtgtaatt gcctgagcgg
     3361 cttcctgctt cgtttccggc agcgcggacg gcgtgaccag cgcccgccgg ttttcaggcg
     3421 cgttcgggtc atgcagcccc agcctgccgt tcacgatggt ctgccaggca tcaatgcgcg
     3481 gacggtcggc gcggtcgtaa tacggctgga gacgcttgcc agtcagcagc tccgtgttcg
     3541 ggatcaggaa gttcagctcc agccgcccct tatcctggtg ctcaacccac agcacgctgt
     3601 actggtcttt gtcgagtccg ggcatcagaa cccgttcaaa gctcgccatc agcttttcgc
     3661 gctgcccggg cggtaaatcc ttttcggcaa aggacaggac gccggatgtg tacttttttg
     3721 cgtagggtga ggcgtcgata agctccctga cttcttccgg cttcccctgc agaacggtgg
     3781 cgccctcgcg ctgtcggtct ttccccagca gataatccac cggaccggcg ccaccgccgc
     3841 gccctcgcgg gtgaaactta acgatcatcg tctgcaccct tttccagcac ggcatgccgc
     3901 agccgctcga gtccggcatc tatggccatc agcgcggcga cgacctgcac gcggtcgtgc
     3961 ccgctgccac caccggcgtt aacctgccgg gcaatctggt taaggttgtt ccccatgccg
     4021 gcaagctgac gaagcagcgc cggcgagatc gagggaagct tgccggcgcg ggcgggtttc
     4081 tcgtccaggc acgtctggcg catccaggct gcaagctgtt taccgtcgca gcgctccagc
     4141 agacgccggt gctcatcctc ggtcacccac atcgtgagca ttttgctgcg tttgtctgcc
     4201 aatccggcct cctgataacg ccgggacggg cgggaaatct tttcccggtc ggcgcggtgt
     4261 tggacaagcc gcaggcgcgt cagtggtttt agcgggtttc ggggcgcagc cctgaaccag
     4321 tcacgtagcg ctagcggagt gtatactggc ttaatatggt aaatcgaagt gggaattcag
     4381 ggaagtgctt catgtggcag gtaaaaaatg cgtctgtggg gcgtaagtgc gaaatgctgg
     4441 ggacgatgaa atgacgcctc ctcgctcact cggtcgctac gctcctgccg tgagactgcg
     4501 gcgggcgtta ccggctcacg aatggcggga taaagagatg gcgctcaaat caggaagggc
     4561 tgggaaaagg atgcggcgtt gccgtttttc cataggctcc gcccccctga caagcatcac
     4621 gaaatatgac gctcaaatca gtggcggcga aacccgacag gactataaag atcccaggcg
     4681 tttccccctg gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     4741 attccgctgt tatgtggccg
//
