LOCUS       NZ_AP025930             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80381 plasmid pFJMB80381_3,
            complete sequence.
ACCESSION   NZ_AP025930
VERSION     NZ_AP025930.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490892
            Assembly: GCF_023735995.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 158x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735995.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:25:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,311
            CDSs (total)                      :: 5,196
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 28 of 96
            Pseudo Genes (incomplete)         :: 72 of 96
            Pseudo Genes (internal stop)      :: 12 of 96
            Pseudo Genes (multiple problems)  :: 14 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025930.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80381"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80381_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-08-20"
     gene            440..583
                     /locus_tag="NGK75_RS26505"
                     /old_locus_tag="FJMB80381_52190"
     CDS             440..583
                     /locus_tag="NGK75_RS26505"
                     /old_locus_tag="FJMB80381_52190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1011..1238
                     /locus_tag="NGK75_RS26510"
                     /old_locus_tag="FJMB80381_52200"
     CDS             1011..1238
                     /locus_tag="NGK75_RS26510"
                     /old_locus_tag="FJMB80381_52200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1605..1790
                     /locus_tag="NGK75_RS26515"
                     /old_locus_tag="FJMB80381_52210"
     CDS             1605..1790
                     /locus_tag="NGK75_RS26515"
                     /old_locus_tag="FJMB80381_52210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1838..1917)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          45..605
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 cagaaaatga aagaaaacgc gtcaggagaa aatgtaatac aggatatatt ccgcttcctc
       61 gctcactcac tcgctgcgct cggtcgttcg gctgcggcga gcggtacggg cttacgggtg
      121 gagcagaaat ttcctagggg atgccagcta ggggatgcca ggaagatact tagcgaagaa
      181 ggcataaggc cgcgccgaag ccgtttttcc ataggctccg cccccctgac gagcatcacg
      241 aaatctgacg ctcaaatcag tggtggcgaa acccgatgcg gcttaaagat accaggcgtt
      301 tccccctgat ggctccctcg tgctcccccg cattcctgcc tttcggttta ccggtgtcac
      361 tccgctgtta tggccgcggt ttctcattcc acgcctgaca ctcggttccg ggaaggcagt
      421 tcgctccagg atggactgta tgcacgaacc ccccgttcag tccgactgct gcgccttatc
      481 cggtaactat cgtcttgagt ccaacccgga aagacacgta aaaacgccac tggcagcggc
      541 cactggtaag gggtatacaa gattactggt gcagagttct tgaagttgac gcctgactgc
      601 ggctacacta gaaggacaaa tttggtggca gcggtcttgt acaccctgtt accacggttc
      661 agaaacttgc cggtttgctg aaccttcgaa aaaccacctt gccgggtggt tttttcgttt
      721 acagggcaag agattacgcg cgaaaaaaaa ggatctcaag aagatccttt tataaattag
      781 acatccaacc tcacaaagag gaatacttaa atcatcttgg tatatgtatc ggtcggatta
      841 catcagatat aagcccgcac tgccccctcc ccgccagaca ccacacttta accataacac
      901 ctcgcaccta actcgatatg cagcccacca tcaagcctct tttcacataa aaaatgacct
      961 atagttagat ggttagtgac caaaactgat atggataaaa ggagctggct ttgaaaaaga
     1021 tttttgtttc cgtgttcgca gcggcagttg cgctttcagc cctcaccggc tgcacgcgca
     1081 caagctatgc catacacacc aatgacgggc gcactattgt cagcgacggc aaacctaaag
     1141 aatcagactc aggcctgctt ggctatacag acgcgaatgg tgtcaaacag cagatcaata
     1201 aaaccgacgt taaagaagtc tccgaaattc cgcactgagc atcttctccg aaaaaggccg
     1261 ctaatgtggc ctttttcttt acctggggtt ctccgccaaa atcccagcaa attgcaccac
     1321 caaagctaaa agcttccagg gttgtctaaa aagcacaaga cattagagga agtgcggttt
     1381 ttataatcaa gccccccaag aatttaccat aacatccgtt gcccgcaccg cctcagacgc
     1441 actcagcgct tttctgacgg aaccatgacc aaaaaccaca acaaaccacc ccggaacccg
     1501 ccagaaacgc tcctgttaaa ttttaacggc atgcatgact atgcaccaga atgacggcat
     1561 gctcttttca cgcaaaaatc atcaccagac ggggaaaatc accagtgacc agacaggaat
     1621 ccgccgccct caacatggcc aaatttatcc gctcacagac actcctcctc ctcgagcggc
     1681 tcgaacagat ggatctggat gaggctgccg gctgctgtga gcacctgcac gatcaggccg
     1741 aagcacttta caccatgctg aacgcacaga caggcgagga agatgcgtga aaatcggtga
     1801 acgggtgcgc aattcagtgc gcggccgtga ggcgatggcg gggtgtcggg gcgcagccct
     1861 gacctgggta tttgtgatgc cggcgcgtgc gcggtattac aaatacacat cctgtcctga
     1921 gtggttcccc gggaacaggc aaaaaaaaac cggggagctg cccggaaggt aagtcaagtt
     1981 agattagatt actttcactc atccataaca gcatcatgca acgacgacca tcgtccgtga
     2041 cggccgcctc gtttaagtat ggacagaaat acagaaaatg ctcaggacga aatgtaatga
     2101 atgcgaacgg attcaagaaa ttcgagcatg accgtcctta cggccggttc agtttcagac
     2161 aaaatctgcc ggtatgcatc cagcatcatg gctccggcat ccccccggca cgccggagcc
     2221 agcccgaaac aacagacaca aacaggtgtc gctcatcatc aagagtcatc aggtctccgg
     2281 aagaaaaacc aaacatggct tacctcctgt taacggcaac gcagaagaaa ggtttacagg
     2341 gtagcgggca ggaaaataaa aaaattgcag tcagcccgga acggctgctg aaccggtaca
     2401 ggcgggttcc gggcgcagcg ccggaccagt cactcagcgc tagcggagtg tatactggct
     2461 aaaatgtaac accgcagtaa cgcatagcat aactgctaca taagacaggc g
//
