LOCUS       NZ_AP025929             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80381 plasmid pFJMB80381_2,
            complete sequence.
ACCESSION   NZ_AP025929
VERSION     NZ_AP025929.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490892
            Assembly: GCF_023735995.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 158x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735995.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:25:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,311
            CDSs (total)                      :: 5,196
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 28 of 96
            Pseudo Genes (incomplete)         :: 72 of 96
            Pseudo Genes (internal stop)      :: 12 of 96
            Pseudo Genes (multiple problems)  :: 14 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025929.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80381"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80381_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-08-20"
     gene            join(4664..4760,1..365)
                     /locus_tag="NGK75_RS26470"
     CDS             join(4664..4760,1..365)
                     /locus_tag="NGK75_RS26470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(620..730)
                     /locus_tag="NGK75_RS26475"
     CDS             complement(620..730)
                     /locus_tag="NGK75_RS26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            1374..1697
                     /locus_tag="NGK75_RS26480"
                     /old_locus_tag="FJMB80381_52160"
     CDS             1374..1697
                     /locus_tag="NGK75_RS26480"
                     /old_locus_tag="FJMB80381_52160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1906..>2340
                     /locus_tag="NGK75_RS26485"
                     /pseudo=""
     CDS             1906..>2340
                     /locus_tag="NGK75_RS26485"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2375..2860
                     /locus_tag="NGK75_RS26490"
                     /old_locus_tag="FJMB80381_52170"
     CDS             2375..2860
                     /locus_tag="NGK75_RS26490"
                     /old_locus_tag="FJMB80381_52170"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2864..3079
                     /locus_tag="NGK75_RS26495"
                     /old_locus_tag="FJMB80381_52180"
     CDS             2864..3079
                     /locus_tag="NGK75_RS26495"
                     /old_locus_tag="FJMB80381_52180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3350..4264
                     /gene="lpxO"
                     /locus_tag="NGK75_RS26500"
     CDS             3350..4264
                     /gene="lpxO"
                     /locus_tag="NGK75_RS26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     Typing          1087..1270
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(621..1104)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          1688..3177
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcaagttat gggggatttt tcgacagagt cgcctagctt tgaaacgcca atgtttgggc
       61 gatattttga tacctttcaa gctgctatac atgttgcaaa agctttccca gggagtacgt
      121 tgcagccatc agagtacgaa gataaaactg ggatgctaat acgtcattat tatgtggatt
      181 gtcatccgta ttatgatcga ctccctcctg aaaaaagcga taattttcag tatgatgagt
      241 acccttctgc tattcatgtg gctgcgtgta ttgatcaagc tagcaaagaa ctcgacgaac
      301 tattgtttgg tggtggatat ggggaaaatg gtgaggggca tgcaagcccg ataattttat
      361 attagtgtta ggggcggagg gatattatcc aatttgtaaa atgatcttct tgagatcgtt
      421 ttgctctgcg cgtaatctct tgctctgaaa acgaaaaaac cacctgggga ggtggttttt
      481 cgaaggttca gtaagttggg gaacttctga accgtggtaa cagtcttgtg cgagacgtgt
      541 caccaaatct gtccttccag tgtagccgta gtccggccac cacttcaaga actctcgata
      601 catctctcgc acatcctgct taccagtggc taccgccaat ggcgattttg tcgtgtcttt
      661 ccgggttgga ctcaagttga tagttaccgg aatgggcgtg gtagtcggac tgaacggggg
      721 ggtcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      781 gaatgagaca aacgcggcca cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
      841 caggagagcg cacgagggag ctaccagggg gaaacgcctg ggatctttat agtcctgtcg
      901 ggtttcgccg ccactgattt gagcgtcata tttcgtgatg cttgtcaggg gggcggagcc
      961 tatggaaaaa cggcaacgcc gcatcctttt cccagccctt cctgatttga gcgccatctc
     1021 tttatcccgc cattcgtgag ccggtaacgc ccgccgcagt ctcacggcag gagcgtagcg
     1081 accgagtgag cgaggaggcg tcatttcatc gtccccagca tttcgcactt acgccccaca
     1141 gacgcatttt ttacctgcca catgaagcac ttccctgaat tcccacttcg atttaccata
     1201 ttaagccagt atacactccg ctagcgctac gtgactggtt cagggctgcg ccccgaaacc
     1261 cgctaaaacc actgacgcgc ctgcggcttg tccaacaccg cgccgaccgg gaaaagattt
     1321 cccgcccgtc ccggcgttat caggaggccg gattggcaga caaacgcagc aaaatgctca
     1381 cgatgtgggt gaccgaggat gagcaccggc gtctgctgga gcgctgcgac ggtaaacagc
     1441 ttgcagcctg gatgcgccag acgtgcctgg acgagaaacc cgcccgcgcc ggcaagcttc
     1501 cctcgatctc gccggcgctg cttcgtcagc ttgccggcat ggggaacaac cttaaccaga
     1561 ttgcccggca ggttaacgcc ggtggtggca gcgggcacga ccgcgtgcag gtcgtcgccg
     1621 cgctgatggc catagatgcc ggactcgagc ggctgcggca tgccgtgctg gaaaagggtg
     1681 cagacgatga tcgttaagtt tcacccgcga gggcgcggcg gtggcgccgg tccggtggat
     1741 tatctgctgg ggaaagaccg acagcgcgag ggcgccaccg ttctgcaggg gaagccggaa
     1801 gaagtcaggg agcttatcga cgcctcaccc tacgcaaaaa agtacacatc cggcgtcctg
     1861 tcctttgccg aaaaggattt accgcccggg cagcgcgaaa agctgatggc gagctttgaa
     1921 cgggttctga tgcccggact cgacaaagac cagtacagcg tgctgtgggt tgagcaccag
     1981 gataaggggc ggctggagct gaacttcctg atcccgaaca cggagctgct gactggcaag
     2041 cgtctccagc cgtattacga ccgcgccgac cgtccgcgca ttgatgcctg gcagaccatc
     2101 gtgaacggca ggctggggct gcatgacccg aacgcgcctg aaaaccggcg ggcgctggtc
     2161 acgccgtccg cgctgccgga aacgaagcag gaagccgctc aggcaattac acggggttta
     2221 ctggccttgg cctcgtccgg ggagctgaaa acccgccagg acgtcactga ggcgctggaa
     2281 agcgcaggtt ttgaggtggt acgcaccaca aaaagcagca tcagcattgc cgacccggac
     2341 ggggggcgaa acatccgact gaagggagcc atctatgaac agtcttttaa cgctggcgaa
     2401 ggacttagag cagaaatcga aagcgcagca gcagagtacc ggcgagatgc tgaaagccgc
     2461 attcagcgag cacgagaagt ctgtcagagc ggaactgagc gaaagcgaga agagaatcag
     2521 cgccgccatc ctcgaccacg accggaagct gtcctcagcc atgagccagc gtacgaaagg
     2581 gatgctgcgc atggtcagcc agacgtggct gaccatcgtc ctggtctccg tgctgctgat
     2641 cgcctcgaac gcggccattc tgtggtggca gagtcagcag atactcgaca attacgtgag
     2701 catccgggag cagaagagca cgcaggcgat gctgtccgag cggaacagcg gcgtgcagct
     2761 ctcgacctgc ggcgagcaga gacgccgctg cgtgagggtg aacccggaag cgggacggtt
     2821 cggagaggat tcgagctgga tgatactggc ggggaaatag cacatgacag agctggaaaa
     2881 acagttgctg agcgcattag agcagctaca gcaggactac tcgaaaaggc tggacgagtg
     2941 ggagagcgcc ttcgcggaat ggcggacgat gtctggtctt atgcaacggg agaacgcggc
     3001 gctgagcgag cgcgtcacgg gcttgagcag gcaggtgcag agtttgagcg atcagctgcg
     3061 ccggttgtcg agcggctgaa tgccgtcgag ctgcagcagg tgcgggaggc gcggcatcag
     3121 aaagcgctgg agctcgaacg atcacagcga cagcgggtat atcacggtcc gtccctttga
     3181 ttcatttcag cataattcct gctgaaatcc tctctgcctg tgtagtagat atgttctggt
     3241 gaagcttcga ttatactatt gataaatagc atttagatta taatttaagt gcattcatta
     3301 tgttatcttt aagagatttt tattattggc gtgaacaagg aatatacaca tgctgtcact
     3361 tattattatt tttgtttttc ttatcagtgt attgtatgcg cattctcgcg gtaaaataaa
     3421 acaaaagttt tccaggcagt tatttgatca ttcaacattt atggctccga taaatatgtt
     3481 tatgacaggc ttttcgaagc tgccgtcaaa gcaggcatgg tttcccccag aaacatttcc
     3541 tgaacttcaa ccattaattg ataactggca ggttataaga gacgaagctg ttaatttgca
     3601 agggcacatt aaatcatctg aaaaaaataa tgatgcagga tttaatacat tttttaagcg
     3661 cggttggaag cgcttttacc tcaagtggta tggagatgct catccatcag cggagcggct
     3721 ttgcccagta acaacccaac tggttagctc tattccctgt attaaagcag cgatgtttgc
     3781 tgaattgcct tcaggaagcc atttaggaaa gcatcgtgac ccttatgccg gttcagttcg
     3841 ttatcattta ggattatcaa caccaaatga tgaacggtgt tttattgaag ttgatggtgt
     3901 acgccgcagt tggcatgatg gtgaagtgat aatttttgac gaaacttttg ttcactgggc
     3961 tcagaatgat accgagcaga cacgtattat tttgttctgt gatatagaaa ggccgatgaa
     4021 gtggcggtgg tcacaaaaaa taaataggtg gataggtggg gtgctaatgt cagcggcagc
     4081 atcacccaac gataataatg acaggactgg tgctataaat catttattta aatatgttaa
     4141 tgctattcgt gatgcaggtc agaagctaaa aaaaaggaat cgaaaactat attatttgat
     4201 taaaaatgga gttatttctt tgatttttgt ttggcttgct gtctcagtgt ttagatggct
     4261 gtgatttgtt tttttctatt gagtcataca taaatttggg tcttctatgc tcaatgtatg
     4321 gttatagata aaaatcgcct gctaaaaaat tctattgaaa tctttttagt aagaaaggcc
     4381 cgaatctgac gctcagcggg cctttcgtga agaagtgcgg cagtagacag atttcatctg
     4441 atcacgttca tgtgctgctg tgcaattaag cgtttctttc ggagctttca cggtatttcc
     4501 ttcacgaaac cggccgccat tcactcttgt gcatagccat gcagccgcgc agaaattatc
     4561 aggggcgttt ttcagggttt gggccgggga aaggtgccgg aaacggccct caggccgcag
     4621 aaacgcgctg aggggctctc agggggtgcg gccaagaagc gttatggtaa atacatatta
     4681 tggttattta ttaggagtaa ctctgggaga gtgggctatg ggatcagatg ttcaatggca
     4741 gatagataat ggttatttgg
//
