LOCUS       NZ_AP025926             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80380 plasmid pFJMB80380_3,
            complete sequence.
ACCESSION   NZ_AP025926
VERSION     NZ_AP025926.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490891
            Assembly: GCF_023735975.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 143x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735975.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:16:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,184
            CDSs (total)                      :: 5,069
            Genes (coding)                    :: 4,980
            CDSs (with protein)               :: 4,980
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 28 of 89
            Pseudo Genes (incomplete)         :: 65 of 89
            Pseudo Genes (internal stop)      :: 12 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            Pseudo Genes (short protein)      :: 1 of 89
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025926.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80380"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80380_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-07-11"
     gene            complement(803..988)
                     /locus_tag="NGK76_RS25865"
                     /old_locus_tag="FJMB80380_51050"
     CDS             complement(803..988)
                     /locus_tag="NGK76_RS25865"
                     /old_locus_tag="FJMB80380_51050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1355..1582)
                     /locus_tag="NGK76_RS25870"
                     /old_locus_tag="FJMB80380_51060"
     CDS             complement(1355..1582)
                     /locus_tag="NGK76_RS25870"
                     /old_locus_tag="FJMB80380_51060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(2010..2153)
                     /locus_tag="NGK76_RS25875"
                     /old_locus_tag="FJMB80380_51070"
     CDS             complement(2010..2153)
                     /locus_tag="NGK76_RS25875"
                     /old_locus_tag="FJMB80380_51070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     Typing          677..756
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(1989..2511)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.689"
                     /coverage="85.0"
                     /evalue="5.38e-168"
ORIGIN
        1 gagcgcagcg agtgagtgag cgaggaagcg gaatatatcc tgtattacat tttctcctga
       61 cgcgttttct ttcattttct gcgcctgtct tatgtagcag ttatgctatg cgttactgcg
      121 gtgttacatt ttagccagta tacactccgc tagcgctgag tgactggtcc ggcgctgcgc
      181 ccggaacccg cctgtaccgg ttcagcagcc gttccgggct gactgcaatt tttttatttt
      241 cctgcccgct accctgtaaa cctttcttct gcgttgccgt taacaggagg taagccatgt
      301 ttggtttttc ttccggagac ctgatgactc ttgatgatga gcgacacctg tttgtgtctg
      361 ttgtttcggg ctggctccgg cgtgccgggg ggatgccgga gccatgatgc tggatgcata
      421 ccggcagatt ttgtctgaaa ctgaaccggc cgtaaggacg gtcatgctcg aatttcttga
      481 atccgttcgc attcattaca tttcgtcctg agcattttct gtatttctgt ccatacttaa
      541 acgaggcggc cgtcacggac gatggtcgtc gttgcatgat gctgttatgg atgagtgaaa
      601 gtaatctaat ctaacttgac ttaccttccg ggcagctccc cggttttttt ttgcctgttc
      661 ccggggaacc actcaggaca ggatgtgtat ttgtaatacc gcgcacgcgc cggcatcaca
      721 aatacccagg tcagggctgc gccccgacac cccgccatcg cctcacggcc gcgcactgaa
      781 ttgcgcaccc gttcaccgat tttcacgcat cttcctcgcc tgtctgtgcg ttcagcatgg
      841 tgtaaagtgc ttcggcctga tcgtgcaggt gctcacagca gccggcagcc tcatccagat
      901 ccatctgttc gagccgctcg aggaggagga gtgtctgtga gcggataaat ttggccatgt
      961 tgagggcggc ggattcctgt ctggtcactg gtgattttcc ccgtctggtg atgatttttg
     1021 cgtgaaaaga gcatgccgtc attctggtgc atagtcatgc atgccgttaa aatttaacag
     1081 gagcgtttct ggcgggttcc ggggtggttt gttgtggttt ttggtcatgg ttccgtcaga
     1141 aaagcgctga gtgcgtctga ggcggtgcgg gcaacggatg ttatggtaaa ttcttggggg
     1201 gcttgattat aaaaaccgca cttcctctaa tgtcttgtgc tttttagaca accctggaag
     1261 cttttagctt tggtggtgca atttgctggg attttggcgg agaaccccag gtaaagaaaa
     1321 aggccacatt agcggccttt ttcggagaag atgctcagtg cggaatttcg gagacttctt
     1381 taacgtcggt tttattgatc tgctgtttga caccattcgc gtctgtatag ccaagcaggc
     1441 ctgagtctga ttctttaggt ttgccgtcgc tgacaatagt gcgcccgtca ttggtgtgta
     1501 tggcatagct tgtgcgcgtg cagccggtga gggctgaaag cgcaactgcc gctgcgaaca
     1561 cggaaacaaa aatctttttc aaagccagct ccttttatcc atatcagttt tggtcactaa
     1621 ccatctaact ataggtcatt ttttatgtga aaagaggctt gatggtgggc tgcatatcga
     1681 gttaggtgcg aggtgttatg gttaaagtgt ggtgtctggc ggggaggggg cagtgcgggc
     1741 ttatatctga tgtaatccga ccgatacata taccaagatg atttaagtat tcctctttgt
     1801 gaggttggat gtctaattta taaaaggatc ttcttgagat cctttttttt cgcgcgtaat
     1861 ctcttgccct gtaaacgaaa aaaccacccg gcaaggtggt ttttcgaagg ttcagcaaac
     1921 cggcaagttt ctgaaccgtg gtaacagggt gtacaagacc gctgccacca aatttgtcct
     1981 tctagtgtag ccgcagtcag gcgtcaactt caagaactct gcaccagtaa tcttgtatac
     2041 cccttaccag tggccgctgc cagtggcgtt tttacgtgtc tttccgggtt ggactcaaga
     2101 cgatagttac cggataaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc
     2161 atcctggagc gaactgcctt cccggaaccg agtgtcaggc gtggaatgag aaaccgcggc
     2221 cataacagcg gagtgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag
     2281 ccatcagggg gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg
     2341 agcgtcagat ttcgtgatgc tcgtcagggg ggcggagcct atggaaaaac ggcttcggcg
     2401 cggccttatg ccttcttcgc taagtatctt cctggcatcc cctagctggc atcccctagg
     2461 aaatttctgc tccacccgta agcccgtacc gctcgccgca gccgaacgac c
//
