LOCUS       NZ_AP025925             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80380 plasmid pFJMB80380_2,
            complete sequence.
ACCESSION   NZ_AP025925
VERSION     NZ_AP025925.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490891
            Assembly: GCF_023735975.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 143x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735975.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:16:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,184
            CDSs (total)                      :: 5,069
            Genes (coding)                    :: 4,980
            CDSs (with protein)               :: 4,980
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 28 of 89
            Pseudo Genes (incomplete)         :: 65 of 89
            Pseudo Genes (internal stop)      :: 12 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            Pseudo Genes (short protein)      :: 1 of 89
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025925.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80380"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80380_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-07-11"
     gene            join(4617..4760,1..318)
                     /locus_tag="NGK76_RS25830"
     CDS             join(4617..4760,1..318)
                     /locus_tag="NGK76_RS25830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(573..683)
                     /locus_tag="NGK76_RS25835"
     CDS             complement(573..683)
                     /locus_tag="NGK76_RS25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            1327..1650
                     /locus_tag="NGK76_RS25840"
                     /old_locus_tag="FJMB80380_51010"
     CDS             1327..1650
                     /locus_tag="NGK76_RS25840"
                     /old_locus_tag="FJMB80380_51010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1859..>2293
                     /locus_tag="NGK76_RS25845"
                     /pseudo=""
     CDS             1859..>2293
                     /locus_tag="NGK76_RS25845"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2328..2813
                     /locus_tag="NGK76_RS25850"
                     /old_locus_tag="FJMB80380_51020"
     CDS             2328..2813
                     /locus_tag="NGK76_RS25850"
                     /old_locus_tag="FJMB80380_51020"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2817..3032
                     /locus_tag="NGK76_RS25855"
                     /old_locus_tag="FJMB80380_51030"
     CDS             2817..3032
                     /locus_tag="NGK76_RS25855"
                     /old_locus_tag="FJMB80380_51030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3303..4217
                     /gene="lpxO"
                     /locus_tag="NGK76_RS25860"
     CDS             3303..4217
                     /gene="lpxO"
                     /locus_tag="NGK76_RS25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     Typing          1040..1223
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(574..1057)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          1641..3130
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccaatgtttg ggcgatattt tgataccttt caagctgcta tacatgttgc aaaagctttc
       61 ccagggagta cgttgcagcc atcagagtac gaagataaaa ctgggatgct aatacgtcat
      121 tattatgtgg attgtcatcc gtattatgat cgactccctc ctgaaaaaag cgataatttt
      181 cagtatgatg agtacccttc tgctattcat gtggctgcgt gtattgatca agctagcaaa
      241 gaactcgacg aactattgtt tggtggtgga tatggggaaa atggtgaggg gcatgcaagc
      301 ccgataattt tatattagtg ttaggggcgg agggatatta tccaatttgt aaaatgatct
      361 tcttgagatc gttttgctct gcgcgtaatc tcttgctctg aaaacgaaaa aaccacctgg
      421 ggaggtggtt tttcgaaggt tcagtaagtt ggggaacttc tgaaccgtgg taacagtctt
      481 gtgcgagacg tgtcaccaaa tctgtccttc cagtgtagcc gtagtccggc caccacttca
      541 agaactctcg atacatctct cgcacatcct gcttaccagt ggctaccgcc aatggcgatt
      601 ttgtcgtgtc tttccgggtt ggactcaagt tgatagttac cggaatgggc gtggtagtcg
      661 gactgaacgg gggggtcgtg catacagtcc agcttggagc gaactgccta cccggaactg
      721 agtgtcaggc gtggaatgag acaaacgcgg ccacataaca gcggaatgac accggtaaac
      781 cgaaaggcag gaacaggaga gcgcacgagg gagctaccag ggggaaacgc ctgggatctt
      841 tatagtcctg tcgggtttcg ccgccactga tttgagcgtc atatttcgtg atgcttgtca
      901 ggggggcgga gcctatggaa aaacggcaac gccgcatcct tttcccagcc cttcctgatt
      961 tgagcgccat ctctttatcc cgccattcgt gagccggtaa cgcccgccgc agtctcacgg
     1021 caggagcgta gcgaccgagt gagcgaggag gcgtcatttc atcgtcccca gcatttcgca
     1081 cttacgcccc acagacgcat tttttacctg ccacatgaag cacttccctg aattcccact
     1141 tcgatttacc atattaagcc agtatacact ccgctagcgc tacgtgactg gttcagggct
     1201 gcgccccgaa acccgctaaa accactgacg cgcctgcggc ttgtccaaca ccgcgccgac
     1261 cgggaaaaga tttcccgccc gtcccggcgt tatcaggagg ccggattggc agacaaacgc
     1321 agcaaaatgc tcacgatgtg ggtgaccgag gatgagcacc ggcgtctgct ggagcgctgc
     1381 gacggtaaac agcttgcagc ctggatgcgc cagacgtgcc tggacgagaa acccgcccgc
     1441 gccggcaagc ttccctcgat ctcgccggcg ctgcttcgtc agcttgccgg catggggaac
     1501 aaccttaacc agattgcccg gcaggttaac gccggtggtg gcagcgggca cgaccgcgtg
     1561 caggtcgtcg ccgcgctgat ggccatagat gccggactcg agcggctgcg gcatgccgtg
     1621 ctggaaaagg gtgcagacga tgatcgttaa gtttcacccg cgagggcgcg gcggtggcgc
     1681 cggtccggtg gattatctgc tggggaaaga ccgacagcgc gagggcgcca ccgttctgca
     1741 ggggaagccg gaagaagtca gggagcttat cgacgcctca ccctacgcaa aaaagtacac
     1801 atccggcgtc ctgtcctttg ccgaaaagga tttaccgccc gggcagcgcg aaaagctgat
     1861 ggcgagcttt gaacgggttc tgatgcccgg actcgacaaa gaccagtaca gcgtgctgtg
     1921 ggttgagcac caggataagg ggcggctgga gctgaacttc ctgatcccga acacggagct
     1981 gctgactggc aagcgtctcc agccgtatta cgaccgcgcc gaccgtccgc gcattgatgc
     2041 ctggcagacc atcgtgaacg gcaggctggg gctgcatgac ccgaacgcgc ctgaaaaccg
     2101 gcgggcgctg gtcacgccgt ccgcgctgcc ggaaacgaag caggaagccg ctcaggcaat
     2161 tacacggggt ttactggcct tggcctcgtc cggggagctg aaaacccgcc aggacgtcac
     2221 tgaggcgctg gaaagcgcag gttttgaggt ggtacgcacc acaaaaagca gcatcagcat
     2281 tgccgacccg gacggggggc gaaacatccg actgaaggga gccatctatg aacagtcttt
     2341 taacgctggc gaaggactta gagcagaaat cgaaagcgca gcagcagagt accggcgaga
     2401 tgctgaaagc cgcattcagc gagcacgaga agtctgtcag agcggaactg agcgaaagcg
     2461 agaagagaat cagcgccgcc atcctcgacc acgaccggaa gctgtcctca gccatgagcc
     2521 agcgtacgaa agggatgctg cgcatggtca gccagacgtg gctgaccatc gtcctggtct
     2581 ccgtgctgct gatcgcctcg aacgcggcca ttctgtggtg gcagagtcag cagatactcg
     2641 acaattacgt gagcatccgg gagcagaaga gcacgcaggc gatgctgtcc gagcggaaca
     2701 gcggcgtgca gctctcgacc tgcggcgagc agagacgccg ctgcgtgagg gtgaacccgg
     2761 aagcgggacg gttcggagag gattcgagct ggatgatact ggcggggaaa tagcacatga
     2821 cagagctgga aaaacagttg ctgagcgcat tagagcagct acagcaggac tactcgaaaa
     2881 ggctggacga gtgggagagc gccttcgcgg aatggcggac gatgtctggt cttatgcaac
     2941 gggagaacgc ggcgctgagc gagcgcgtca cgggcttgag caggcaggtg cagagtttga
     3001 gcgatcagct gcgccggttg tcgagcggct gaatgccgtc gagctgcagc aggtgcggga
     3061 ggcgcggcat cagaaagcgc tggagctcga acgatcacag cgacagcggg tatatcacgg
     3121 tccgtccctt tgattcattt cagcataatt cctgctgaaa tcctctctgc ctgtgtagta
     3181 gatatgttct ggtgaagctt cgattatact attgataaat agcatttaga ttataattta
     3241 agtgcattca ttatgttatc tttaagagat ttttattatt ggcgtgaaca aggaatatac
     3301 acatgctgtc acttattatt atttttgttt ttcttatcag tgtattgtat gcgcattctc
     3361 gcggtaaaat aaaacaaaag ttttccaggc agttatttga tcattcaaca tttatggctc
     3421 cgataaatat gtttatgaca ggcttttcga agctgccgtc aaagcaggca tggtttcccc
     3481 cagaaacatt tcctgaactt caaccattaa ttgataactg gcaggttata agagacgaag
     3541 ctgttaattt gcaagggcac attaaatcat ctgaaaaaaa taatgatgca ggatttaata
     3601 cattttttaa gcgcggttgg aagcgctttt acctcaagtg gtatggagat gctcatccat
     3661 cagcggagcg gctttgccca gtaacaaccc aactggttag ctctattccc tgtattaaag
     3721 cagcgatgtt tgctgaattg ccttcaggaa gccatttagg aaagcatcgt gacccttatg
     3781 ccggttcagt tcgttatcat ttaggattat caacaccaaa tgatgaacgg tgttttattg
     3841 aagttgatgg tgtacgccgc agttggcatg atggtgaagt gataattttt gacgaaactt
     3901 ttgttcactg ggctcagaat gataccgagc agacacgtat tattttgttc tgtgatatag
     3961 aaaggccgat gaagtggcgg tggtcacaaa aaataaatag gtggataggt ggggtgctaa
     4021 tgtcagcggc agcatcaccc aacgataata atgacaggac tggtgctata aatcatttat
     4081 ttaaatatgt taatgctatt cgtgatgcag gtcagaagct aaaaaaaagg aatcgaaaac
     4141 tatattattt gattaaaaat ggagttattt ctttgatttt tgtttggctt gctgtctcag
     4201 tgtttagatg gctgtgattt gtttttttct attgagtcat acataaattt gggtcttcta
     4261 tgctcaatgt atggttatag ataaaaatcg cctgctaaaa aattctattg aaatcttttt
     4321 agtaagaaag gcccgaatct gacgctcagc gggcctttcg tgaagaagtg cggcagtaga
     4381 cagatttcat ctgatcacgt tcatgtgctg ctgtgcaatt aagcgtttct ttcggagctt
     4441 tcacggtatt tccttcacga aaccggccgc cattcactct tgtgcatagc catgcagccg
     4501 cgcagaaatt atcaggggcg tttttcaggg tttgggccgg ggaaaggtgc cggaaacggc
     4561 cctcaggccg cagaaacgcg ctgaggggct ctcagggggt gcggccaaga agcgttatgg
     4621 taaatacata ttatggttat ttattaggag taactctggg agagtgggct atgggatcag
     4681 atgttcaatg gcagatagat aatggttatt tggcgcaagt tatgggggat ttttcgacag
     4741 agtcgcctag ctttgaaacg
//
