LOCUS       NZ_AP025922             2511 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80378 plasmid pFJMB80378_3,
            complete sequence.
ACCESSION   NZ_AP025922
VERSION     NZ_AP025922.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490890
            Assembly: GCF_023735955.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 142x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735955.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:55:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,308
            CDSs (total)                      :: 5,193
            Genes (coding)                    :: 5,094
            CDSs (with protein)               :: 5,094
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 27 of 99
            Pseudo Genes (incomplete)         :: 75 of 99
            Pseudo Genes (internal stop)      :: 13 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            Pseudo Genes (short protein)      :: 1 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025922.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80378"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80378_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-04-17"
     gene            complement(394..537)
                     /locus_tag="NGK71_RS26490"
                     /old_locus_tag="FJMB80378_52250"
     CDS             complement(394..537)
                     /locus_tag="NGK71_RS26490"
                     /old_locus_tag="FJMB80378_52250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(1698..1883)
                     /locus_tag="NGK71_RS26495"
                     /old_locus_tag="FJMB80378_52270"
     CDS             complement(1698..1883)
                     /locus_tag="NGK71_RS26495"
                     /old_locus_tag="FJMB80378_52270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2250..2477)
                     /locus_tag="NGK71_RS26500"
                     /old_locus_tag="FJMB80378_52280"
     CDS             complement(2250..2477)
                     /locus_tag="NGK71_RS26500"
                     /old_locus_tag="FJMB80378_52280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1572..1651
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(373..933)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ctaaccatct aactataggt cattttttat gtgaaaagag gcttgatggt gggctgcata
       61 tcgagttagg tgcgaggtgt tatggttaaa gtgtggtgtc tggcggggag ggggcagtgc
      121 gggcttatat ctgatgtaat ccgaccgata catataccaa gatgatttaa gtattcctct
      181 ttgtgaggtt ggatgtctaa tttataaaag gatcttcttg agatcctttt ttttcgcgcg
      241 taatctcttg ccctgtaaac gaaaaaacca cccggcaagg tggtttttcg aaggttcagc
      301 aaaccggcaa gtttctgaac cgtggtaaca gggtgtacaa gaccgctgcc accaaatttg
      361 tccttctagt gtagccgcag tcaggcgtca acttcaagaa ctctgcacca gtaatcttgt
      421 atacccctta ccagtggccg ctgccagtgg cgtttttacg tgtctttccg ggttggactc
      481 aagacgatag ttaccggata aggcgcagca gtcggactga acggggggtt cgtgcataca
      541 gtccatcctg gagcgaactg ccttcccgga accgagtgtc aggcgtggaa tgagaaaccg
      601 cggccataac agcggagtga caccggtaaa ccgaaaggca ggaatgcggg ggagcacgag
      661 ggagccatca gggggaaacg cctggtatct ttaagccgca tcgggtttcg ccaccactga
      721 tttgagcgtc agatttcgtg atgctcgtca ggggggcgga gcctatggaa aaacggcttc
      781 ggcgcggcct tatgccttct tcgctaagta tcttcctggc atcccctagc tggcatcccc
      841 taggaaattt ctgctccacc cgtaagcccg taccgctcgc cgcagccgaa cgaccgagcg
      901 cagcgagtga gtgagcgagg aagcggaata tatcctgtat tacattttct cctgacgcgt
      961 tttctttcat tttctgcgcc tgtcttatgt agcagttatg ctatgcgtta ctgcggtgtt
     1021 acattttagc cagtatacac tccgctagcg ctgagtgact ggtccggcgc tgcgcccgga
     1081 acccgcctgt accggttcag cagccgttcc gggctgactg caattttttt attttcctgc
     1141 ccgctaccct gtaaaccttt cttctgcgtt gccgttaaca ggaggtaagc catgtttggt
     1201 ttttcttccg gagacctgat gactcttgat gatgagcgac acctgtttgt gtctgttgtt
     1261 tcgggctggc tccggcgtgc cggggggatg ccggagccat gatgctggat gcataccggc
     1321 agattttgtc tgaaactgaa ccggccgtaa ggacggtcat gctcgaattt cttgaatccg
     1381 ttcgcattca ttacatttcg tcctgagcat tttctgtatt tctgtccata cttaaacgag
     1441 gcggccgtca cggacgatgg tcgtcgttgc atgatgctgt tatggatgag tgaaagtaat
     1501 ctaatctaac ttgacttacc ttccgggcag ctccccggtt tttttttgcc tgttcccggg
     1561 gaaccactca ggacaggatg tgtatttgta ataccgcgca cgcgccggca tcacaaatac
     1621 ccaggtcagg gctgcgcccc gacaccccgc catcgcctca cggccgcgca ctgaattgcg
     1681 cacccgttca ccgattttca cgcatcttcc tcgcctgtct gtgcgttcag catggtgtaa
     1741 agtgcttcgg cctgatcgtg caggtgctca cagcagccgg cagcctcatc cagatccatc
     1801 tgttcgagcc gctcgaggag gaggagtgtc tgtgagcgga taaatttggc catgttgagg
     1861 gcggcggatt cctgtctggt cactggtgat tttccccgtc tggtgatgat ttttgcgtga
     1921 aaagagcatg ccgtcattct ggtgcatagt catgcatgcc gttaaaattt aacaggagcg
     1981 tttctggcgg gttccggggt ggtttgttgt ggtttttggt catggttccg tcagaaaagc
     2041 gctgagtgcg tctgaggcgg tgcgggcaac ggatgttatg gtaaattctt ggggggcttg
     2101 attataaaaa ccgcacttcc tctaatgtct tgtgcttttt agacaaccct ggaagctttt
     2161 agctttggtg gtgcaatttg ctgggatttt ggcggagaac cccaggtaaa gaaaaaggcc
     2221 acattagcgg cctttttcgg agaagatgct cagtgcggaa tttcggagac ttctttaacg
     2281 tcggttttat tgatctgctg tttgacacca ttcgcgtctg tatagccaag caggcctgag
     2341 tctgattctt taggtttgcc gtcgctgaca atagtgcgcc cgtcattggt gtgtatggca
     2401 tagcttgtgc gcgtgcagcc ggtgagggct gaaagcgcaa ctgccgctgc gaacacggaa
     2461 acaaaaatct ttttcaaagc cagctccttt tatccatatc agttttggtc a
//
