LOCUS       NZ_AP025921             4760 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80378 plasmid pFJMB80378_2,
            complete sequence.
ACCESSION   NZ_AP025921
VERSION     NZ_AP025921.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490890
            Assembly: GCF_023735955.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 142x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735955.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:55:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,308
            CDSs (total)                      :: 5,193
            Genes (coding)                    :: 5,094
            CDSs (with protein)               :: 5,094
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 27 of 99
            Pseudo Genes (incomplete)         :: 75 of 99
            Pseudo Genes (internal stop)      :: 13 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            Pseudo Genes (short protein)      :: 1 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025921.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80378"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80378_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-04-17"
     gene            complement(join(4555..4760,1..256))
                     /locus_tag="NGK71_RS26455"
     CDS             complement(join(4555..4760,1..256))
                     /locus_tag="NGK71_RS26455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(656..1570)
                     /gene="lpxO"
                     /locus_tag="NGK71_RS26460"
     CDS             complement(656..1570)
                     /gene="lpxO"
                     /locus_tag="NGK71_RS26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(1841..2056)
                     /locus_tag="NGK71_RS26465"
                     /old_locus_tag="FJMB80378_52220"
     CDS             complement(1841..2056)
                     /locus_tag="NGK71_RS26465"
                     /old_locus_tag="FJMB80378_52220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2060..2545)
                     /locus_tag="NGK71_RS26470"
                     /old_locus_tag="FJMB80378_52230"
     CDS             complement(2060..2545)
                     /locus_tag="NGK71_RS26470"
                     /old_locus_tag="FJMB80378_52230"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2580..3284)
                     /locus_tag="NGK71_RS26575"
                     /pseudo=""
     CDS             complement(<2580..3284)
                     /locus_tag="NGK71_RS26575"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3223..3546)
                     /locus_tag="NGK71_RS26480"
                     /old_locus_tag="FJMB80378_52240"
     CDS             complement(3223..3546)
                     /locus_tag="NGK71_RS26480"
                     /old_locus_tag="FJMB80378_52240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4190..4300
                     /locus_tag="NGK71_RS26485"
     CDS             4190..4300
                     /locus_tag="NGK71_RS26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     Typing          complement(3648..3834)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          3814..4300
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(1744..3233)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttagcatccc agttttatct tcgtactctg atggctgcaa cgtactccct gggaaagctt
       61 ttgcaacatg tatagcagct tgaaaggtat caaaatatcg cccaaacatt ggcgtttcaa
      121 agctaggcga ctctgtcgaa aaatccccca taacttgcgc caaataacca ttatctatct
      181 gccattgaac atctgatccc atagcccact ctcccagagt tactcctaat aaataaccat
      241 aatatgtatt taccataacg cttcttggcc gcaccccctg agagcccctc agcgcgtttc
      301 tgcggcctga gggccgtttc cggcaccttt ccccggccca aaccctgaaa aacgcccctg
      361 ataatttctg cgcggctgca tggctatgca caagagtgaa tggcggccgg tttcgtgaag
      421 gaaataccgt gaaagctccg aaagaaacgc ttaattgcac agcagcacat gaacgtgatc
      481 agatgaaatc tgtctactgc cgcacttctt cacgaaaggc ccgctgagcg tcagattcgg
      541 gcctttctta ctaaaaagat ttcaatagaa ttttttagca ggcgattttt atctataacc
      601 atacattgag catagaagac ccaaatttat gtatgactca atagaaaaaa acaaatcaca
      661 gccatctaaa cactgagaca gcaagccaaa caaaaatcaa agaaataact ccatttttaa
      721 tcaaataata tagttttcga ttcctttttt ttagcttctg acctgcatca cgaatagcat
      781 taacatattt aaataaatga tttatagcac cagtcctgtc attattatcg ttgggtgatg
      841 ctgccgctga cattagcacc ccacctatcc acctatttat tttttgtgac caccgccact
      901 tcatcggcct ttctatatca cagaacaaaa taatacgtgt ctgctcggta tcattctgag
      961 cccagtgaac aaaagtttcg tcaaaaatta tcacttcacc atcatgccaa ctgcggcgta
     1021 caccatcaac ttcaataaaa caccgttcat catttggtgt tgataatcct aaatgataac
     1081 gaactgaacc ggcataaggg tcacgatgct ttcctaaatg gcttcctgaa ggcaattcag
     1141 caaacatcgc tgctttaata cagggaatag agctaaccag ttgggttgtt actgggcaaa
     1201 gccgctccgc tgatggatga gcatctccat accacttgag gtaaaagcgc ttccaaccgc
     1261 gcttaaaaaa tgtattaaat cctgcatcat tatttttttc agatgattta atgtgccctt
     1321 gcaaattaac agcttcgtct cttataacct gccagttatc aattaatggt tgaagttcag
     1381 gaaatgtttc tgggggaaac catgcctgct ttgacggcag cttcgaaaag cctgtcataa
     1441 acatatttat cggagccata aatgttgaat gatcaaataa ctgcctggaa aacttttgtt
     1501 ttattttacc gcgagaatgc gcatacaata cactgataag aaaaacaaaa ataataataa
     1561 gtgacagcat gtgtatattc cttgttcacg ccaataataa aaatctctta aagataacat
     1621 aatgaatgca cttaaattat aatctaaatg ctatttatca atagtataat cgaagcttca
     1681 ccagaacata tctactacac aggcagagag gatttcagca ggaattatgc tgaaatgaat
     1741 caaagggacg gaccgtgata tacccgctgt cgctgtgatc gttcgagctc cagcgctttc
     1801 tgatgccgcg cctcccgcac ctgctgcagc tcgacggcat tcagccgctc gacaaccggc
     1861 gcagctgatc gctcaaactc tgcacctgcc tgctcaagcc cgtgacgcgc tcgctcagcg
     1921 ccgcgttctc ccgttgcata agaccagaca tcgtccgcca ttccgcgaag gcgctctccc
     1981 actcgtccag ccttttcgag tagtcctgct gtagctgctc taatgcgctc agcaactgtt
     2041 tttccagctc tgtcatgtgc tatttccccg ccagtatcat ccagctcgaa tcctctccga
     2101 accgtcccgc ttccgggttc accctcacgc agcggcgtct ctgctcgccg caggtcgaga
     2161 gctgcacgcc gctgttccgc tcggacagca tcgcctgcgt gctcttctgc tcccggatgc
     2221 tcacgtaatt gtcgagtatc tgctgactct gccaccacag aatggccgcg ttcgaggcga
     2281 tcagcagcac ggagaccagg acgatggtca gccacgtctg gctgaccatg cgcagcatcc
     2341 ctttcgtacg ctggctcatg gctgaggaca gcttccggtc gtggtcgagg atggcggcgc
     2401 tgattctctt ctcgctttcg ctcagttccg ctctgacaga cttctcgtgc tcgctgaatg
     2461 cggctttcag catctcgccg gtactctgct gctgcgcttt cgatttctgc tctaagtcct
     2521 tcgccagcgt taaaagactg ttcatagatg gctcccttca gtcggatgtt tcgccccccg
     2581 tccgggtcgg caatgctgat gctgcttttt gtggtgcgta ccacctcaaa acctgcgctt
     2641 tccagcgcct cagtgacgtc ctggcgggtt ttcagctccc cggacgaggc caaggccagt
     2701 aaaccccgtg taattgcctg agcggcttcc tgcttcgttt ccggcagcgc ggacggcgtg
     2761 accagcgccc gccggttttc aggcgcgttc gggtcatgca gccccagcct gccgttcacg
     2821 atggtctgcc aggcatcaat gcgcggacgg tcggcgcggt cgtaatacgg ctggagacgc
     2881 ttgccagtca gcagctccgt gttcgggatc aggaagttca gctccagccg ccccttatcc
     2941 tggtgctcaa cccacagcac gctgtactgg tctttgtcga gtccgggcat cagaacccgt
     3001 tcaaagctcg ccatcagctt ttcgcgctgc ccgggcggta aatccttttc ggcaaaggac
     3061 aggacgccgg atgtgtactt ttttgcgtag ggtgaggcgt cgataagctc cctgacttct
     3121 tccggcttcc cctgcagaac ggtggcgccc tcgcgctgtc ggtctttccc cagcagataa
     3181 tccaccggac cggcgccacc gccgcgccct cgcgggtgaa acttaacgat catcgtctgc
     3241 acccttttcc agcacggcat gccgcagccg ctcgagtccg gcatctatgg ccatcagcgc
     3301 ggcgacgacc tgcacgcggt cgtgcccgct gccaccaccg gcgttaacct gccgggcaat
     3361 ctggttaagg ttgttcccca tgccggcaag ctgacgaagc agcgccggcg agatcgaggg
     3421 aagcttgccg gcgcgggcgg gtttctcgtc caggcacgtc tggcgcatcc aggctgcaag
     3481 ctgtttaccg tcgcagcgct ccagcagacg ccggtgctca tcctcggtca cccacatcgt
     3541 gagcattttg ctgcgtttgt ctgccaatcc ggcctcctga taacgccggg acgggcggga
     3601 aatcttttcc cggtcggcgc ggtgttggac aagccgcagg cgcgtcagtg gttttagcgg
     3661 gtttcggggc gcagccctga accagtcacg tagcgctagc ggagtgtata ctggcttaat
     3721 atggtaaatc gaagtgggaa ttcagggaag tgcttcatgt ggcaggtaaa aaatgcgtct
     3781 gtggggcgta agtgcgaaat gctggggacg atgaaatgac gcctcctcgc tcactcggtc
     3841 gctacgctcc tgccgtgaga ctgcggcggg cgttaccggc tcacgaatgg cgggataaag
     3901 agatggcgct caaatcagga agggctggga aaaggatgcg gcgttgccgt ttttccatag
     3961 gctccgcccc cctgacaagc atcacgaaat atgacgctca aatcagtggc ggcgaaaccc
     4021 gacaggacta taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt
     4081 tcctgccttt cggtttaccg gtgtcattcc gctgttatgt ggccgcgttt gtctcattcc
     4141 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaccc
     4201 ccccgttcag tccgactacc acgcccattc cggtaactat caacttgagt ccaacccgga
     4261 aagacacgac aaaatcgcca ttggcggtag ccactggtaa gcaggatgtg cgagagatgt
     4321 atcgagagtt cttgaagtgg tggccggact acggctacac tggaaggaca gatttggtga
     4381 cacgtctcgc acaagactgt taccacggtt cagaagttcc ccaacttact gaaccttcga
     4441 aaaaccacct ccccaggtgg ttttttcgtt ttcagagcaa gagattacgc gcagagcaaa
     4501 acgatctcaa gaagatcatt ttacaaattg gataatatcc ctccgcccct aacactaata
     4561 taaaattatc gggcttgcat gcccctcacc attttcccca tatccaccac caaacaatag
     4621 ttcgtcgagt tctttgctag cttgatcaat acacgcagcc acatgaatag cagaagggta
     4681 ctcatcatac tgaaaattat cgcttttttc aggagggagt cgatcataat acggatgaca
     4741 atccacataa taatgacgta
//
