LOCUS       NZ_AP025918             2511 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80377 plasmid pFJMB80377_3,
            complete sequence.
ACCESSION   NZ_AP025918
VERSION     NZ_AP025918.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490889
            Assembly: GCF_023735935.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 159x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735935.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:02:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,308
            CDSs (total)                      :: 5,193
            Genes (coding)                    :: 5,093
            CDSs (with protein)               :: 5,093
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 28 of 100
            Pseudo Genes (incomplete)         :: 76 of 100
            Pseudo Genes (internal stop)      :: 12 of 100
            Pseudo Genes (multiple problems)  :: 14 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025918.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80377"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80377_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-03-04"
     gene            308..451
                     /locus_tag="NGK73_RS26490"
     CDS             308..451
                     /locus_tag="NGK73_RS26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            879..1106
                     /locus_tag="NGK73_RS26495"
                     /old_locus_tag="FJMB80377_52030"
     CDS             879..1106
                     /locus_tag="NGK73_RS26495"
                     /old_locus_tag="FJMB80377_52030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1473..1658
                     /locus_tag="NGK73_RS26500"
                     /old_locus_tag="FJMB80377_52040"
     CDS             1473..1658
                     /locus_tag="NGK73_RS26500"
                     /old_locus_tag="FJMB80377_52040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1706..1785)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          23..473
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.132"
                     /coverage="90.0"
                     /evalue="3.24e-165"
ORIGIN
        1 cctaggggat gccagctagg ggatgccagg aagatactta gcgaagaagg cataaggccg
       61 cgccgaagcc gtttttccat aggctccgcc cccctgacga gcatcacgaa atctgacgct
      121 caaatcagtg gtggcgaaac ccgatgcggc ttaaagatac caggcgtttc cccctgatgg
      181 ctccctcgtg ctcccccgca ttcctgcctt tcggtttacc ggtgtcactc cgctgttatg
      241 gccgcggttt ctcattccac gcctgacact cggttccggg aaggcagttc gctccaggat
      301 ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttatccg gtaactatcg
      361 tcttgagtcc aacccggaaa gacacgtaaa aacgccactg gcagcggcca ctggtaaggg
      421 gtatacaaga ttactggtgc agagttcttg aagttgacgc ctgactgcgg ctacactaga
      481 aggacaaatt tggtggcagc ggtcttgtac accctgttac cacggttcag aaacttgccg
      541 gtttgctgaa ccttcgaaaa accaccttgc cgggtggttt tttcgtttac agggcaagag
      601 attacgcgcg aaaaaaaagg atctcaagaa gatcctttta taaattagac atccaacctc
      661 acaaagagga atacttaaat catcttggta tatgtatcgg tcggattaca tcagatataa
      721 gcccgcactg ccccctcccc gccagacacc acactttaac cataacacct cgcacctaac
      781 tcgatatgca gcccaccatc aagcctcttt tcacataaaa aatgacctat agttagatgg
      841 ttagtgacca aaactgatat ggataaaagg agctggcttt gaaaaagatt tttgtttccg
      901 tgttcgcagc ggcagttgcg ctttcagccc tcaccggctg cacgcgcaca agctatgcca
      961 tacacaccaa tgacgggcgc actattgtca gcgacggcaa acctaaagaa tcagactcag
     1021 gcctgcttgg ctatacagac gcgaatggtg tcaaacagca gatcaataaa accgacgtta
     1081 aagaagtctc cgaaattccg cactgagcat cttctccgaa aaaggccgct aatgtggcct
     1141 ttttctttac ctggggttct ccgccaaaat cccagcaaat tgcaccacca aagctaaaag
     1201 cttccagggt tgtctaaaaa gcacaagaca ttagaggaag tgcggttttt ataatcaagc
     1261 cccccaagaa tttaccataa catccgttgc ccgcaccgcc tcagacgcac tcagcgcttt
     1321 tctgacggaa ccatgaccaa aaaccacaac aaaccacccc ggaacccgcc agaaacgctc
     1381 ctgttaaatt ttaacggcat gcatgactat gcaccagaat gacggcatgc tcttttcacg
     1441 caaaaatcat caccagacgg ggaaaatcac cagtgaccag acaggaatcc gccgccctca
     1501 acatggccaa atttatccgc tcacagacac tcctcctcct cgagcggctc gaacagatgg
     1561 atctggatga ggctgccggc tgctgtgagc acctgcacga tcaggccgaa gcactttaca
     1621 ccatgctgaa cgcacagaca ggcgaggaag atgcgtgaaa atcggtgaac gggtgcgcaa
     1681 ttcagtgcgc ggccgtgagg cgatggcggg gtgtcggggc gcagccctga cctgggtatt
     1741 tgtgatgccg gcgcgtgcgc ggtattacaa atacacatcc tgtcctgagt ggttccccgg
     1801 gaacaggcaa aaaaaaaccg gggagctgcc cggaaggtaa gtcaagttag attagattac
     1861 tttcactcat ccataacagc atcatgcaac gacgaccatc gtccgtgacg gccgcctcgt
     1921 ttaagtatgg acagaaatac agaaaatgct caggacgaaa tgtaatgaat gcgaacggat
     1981 tcaagaaatt cgagcatgac cgtccttacg gccggttcag tttcagacaa aatctgccgg
     2041 tatgcatcca gcatcatggc tccggcatcc ccccggcacg ccggagccag cccgaaacaa
     2101 cagacacaaa caggtgtcgc tcatcatcaa gagtcatcag gtctccggaa gaaaaaccaa
     2161 acatggctta cctcctgtta acggcaacgc agaagaaagg tttacagggt agcgggcagg
     2221 aaaataaaaa aattgcagtc agcccggaac ggctgctgaa ccggtacagg cgggttccgg
     2281 gcgcagcgcc ggaccagtca ctcagcgcta gcggagtgta tactggctaa aatgtaacac
     2341 cgcagtaacg catagcataa ctgctacata agacaggcgc agaaaatgaa agaaaacgcg
     2401 tcaggagaaa atgtaataca ggatatattc cgcttcctcg ctcactcact cgctgcgctc
     2461 ggtcgttcgg ctgcggcgag cggtacgggc ttacgggtgg agcagaaatt t
//
