LOCUS       NZ_AP025917             4760 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80377 plasmid pFJMB80377_2,
            complete sequence.
ACCESSION   NZ_AP025917
VERSION     NZ_AP025917.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490889
            Assembly: GCF_023735935.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 159x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735935.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:02:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,308
            CDSs (total)                      :: 5,193
            Genes (coding)                    :: 5,093
            CDSs (with protein)               :: 5,093
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 28 of 100
            Pseudo Genes (incomplete)         :: 76 of 100
            Pseudo Genes (internal stop)      :: 12 of 100
            Pseudo Genes (multiple problems)  :: 14 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025917.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80377"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80377_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-03-04"
     gene            complement(join(4659..4760,1..93))
                     /locus_tag="NGK73_RS26445"
     CDS             complement(join(4659..4760,1..93))
                     /locus_tag="NGK73_RS26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_086623622.1"
                     /translation="MLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENAALSERVT
                     GLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(<56..142)
                     /locus_tag="NGK73_RS26450"
                     /pseudo=""
     CDS             complement(<56..142)
                     /locus_tag="NGK73_RS26450"
                     /inference="COORDINATES: protein motif:HMM:NF033829.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(118..603)
                     /locus_tag="NGK73_RS26455"
                     /old_locus_tag="FJMB80377_52000"
     CDS             complement(118..603)
                     /locus_tag="NGK73_RS26455"
                     /old_locus_tag="FJMB80377_52000"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<638..1342)
                     /locus_tag="NGK73_RS26575"
                     /pseudo=""
     CDS             complement(<638..1342)
                     /locus_tag="NGK73_RS26575"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1281..1604)
                     /locus_tag="NGK73_RS26465"
                     /old_locus_tag="FJMB80377_52010"
     CDS             complement(1281..1604)
                     /locus_tag="NGK73_RS26465"
                     /old_locus_tag="FJMB80377_52010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2248..2358
                     /locus_tag="NGK73_RS26470"
     CDS             2248..2358
                     /locus_tag="NGK73_RS26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(2613..3074)
                     /locus_tag="NGK73_RS26475"
     CDS             complement(2613..3074)
                     /locus_tag="NGK73_RS26475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(3474..4388)
                     /gene="lpxO"
                     /locus_tag="NGK73_RS26480"
     CDS             complement(3474..4388)
                     /gene="lpxO"
                     /locus_tag="NGK73_RS26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(4558..>4716)
                     /locus_tag="NGK73_RS26485"
                     /pseudo=""
     CDS             complement(4558..>4716)
                     /locus_tag="NGK73_RS26485"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     Typing          complement(1706..1892)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          1872..2358
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(3..1291)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.721"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 accagacatc gtccgccatt ccgcgaaggc gctctcccac tcgtccagcc ttttcgagta
       61 gtcctgctgt agctgctcta atgcgctcag caactgtttt tccagctctg tcatgtgcta
      121 tttccccgcc agtatcatcc agctcgaatc ctctccgaac cgtcccgctt ccgggttcac
      181 cctcacgcag cggcgtctct gctcgccgca ggtcgagagc tgcacgccgc tgttccgctc
      241 ggacagcatc gcctgcgtgc tcttctgctc ccggatgctc acgtaattgt cgagtatctg
      301 ctgactctgc caccacagaa tggccgcgtt cgaggcgatc agcagcacgg agaccaggac
      361 gatggtcagc cacgtctggc tgaccatgcg cagcatccct ttcgtacgct ggctcatggc
      421 tgaggacagc ttccggtcgt ggtcgaggat ggcggcgctg attctcttct cgctttcgct
      481 cagttccgct ctgacagact tctcgtgctc gctgaatgcg gctttcagca tctcgccggt
      541 actctgctgc tgcgctttcg atttctgctc taagtccttc gccagcgtta aaagactgtt
      601 catagatggc tcccttcagt cggatgtttc gccccccgtc cgggtcggca atgctgatgc
      661 tgctttttgt ggtgcgtacc acctcaaaac ctgcgctttc cagcgcctca gtgacgtcct
      721 ggcgggtttt cagctccccg gacgaggcca aggccagtaa accccgtgta attgcctgag
      781 cggcttcctg cttcgtttcc ggcagcgcgg acggcgtgac cagcgcccgc cggttttcag
      841 gcgcgttcgg gtcatgcagc cccagcctgc cgttcacgat ggtctgccag gcatcaatgc
      901 gcggacggtc ggcgcggtcg taatacggct ggagacgctt gccagtcagc agctccgtgt
      961 tcgggatcag gaagttcagc tccagccgcc ccttatcctg gtgctcaacc cacagcacgc
     1021 tgtactggtc tttgtcgagt ccgggcatca gaacccgttc aaagctcgcc atcagctttt
     1081 cgcgctgccc gggcggtaaa tccttttcgg caaaggacag gacgccggat gtgtactttt
     1141 ttgcgtaggg tgaggcgtcg ataagctccc tgacttcttc cggcttcccc tgcagaacgg
     1201 tggcgccctc gcgctgtcgg tctttcccca gcagataatc caccggaccg gcgccaccgc
     1261 cgcgccctcg cgggtgaaac ttaacgatca tcgtctgcac ccttttccag cacggcatgc
     1321 cgcagccgct cgagtccggc atctatggcc atcagcgcgg cgacgacctg cacgcggtcg
     1381 tgcccgctgc caccaccggc gttaacctgc cgggcaatct ggttaaggtt gttccccatg
     1441 ccggcaagct gacgaagcag cgccggcgag atcgagggaa gcttgccggc gcgggcgggt
     1501 ttctcgtcca ggcacgtctg gcgcatccag gctgcaagct gtttaccgtc gcagcgctcc
     1561 agcagacgcc ggtgctcatc ctcggtcacc cacatcgtga gcattttgct gcgtttgtct
     1621 gccaatccgg cctcctgata acgccgggac gggcgggaaa tcttttcccg gtcggcgcgg
     1681 tgttggacaa gccgcaggcg cgtcagtggt tttagcgggt ttcggggcgc agccctgaac
     1741 cagtcacgta gcgctagcgg agtgtatact ggcttaatat ggtaaatcga agtgggaatt
     1801 cagggaagtg cttcatgtgg caggtaaaaa atgcgtctgt ggggcgtaag tgcgaaatgc
     1861 tggggacgat gaaatgacgc ctcctcgctc actcggtcgc tacgctcctg ccgtgagact
     1921 gcggcgggcg ttaccggctc acgaatggcg ggataaagag atggcgctca aatcaggaag
     1981 ggctgggaaa aggatgcggc gttgccgttt ttccataggc tccgcccccc tgacaagcat
     2041 cacgaaatat gacgctcaaa tcagtggcgg cgaaacccga caggactata aagatcccag
     2101 gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     2161 gtcattccgc tgttatgtgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
     2221 taggcagttc gctccaagct ggactgtatg cacgaccccc ccgttcagtc cgactaccac
     2281 gcccattccg gtaactatca acttgagtcc aacccggaaa gacacgacaa aatcgccatt
     2341 ggcggtagcc actggtaagc aggatgtgcg agagatgtat cgagagttct tgaagtggtg
     2401 gccggactac ggctacactg gaaggacaga tttggtgaca cgtctcgcac aagactgtta
     2461 ccacggttca gaagttcccc aacttactga accttcgaaa aaccacctcc ccaggtggtt
     2521 ttttcgtttt cagagcaaga gattacgcgc agagcaaaac gatctcaaga agatcatttt
     2581 acaaattgga taatatccct ccgcccctaa cactaatata aaattatcgg gcttgcatgc
     2641 ccctcaccat tttccccata tccaccacca aacaatagtt cgtcgagttc tttgctagct
     2701 tgatcaatac acgcagccac atgaatagca gaagggtact catcatactg aaaattatcg
     2761 cttttttcag gagggagtcg atcataatac ggatgacaat ccacataata atgacgtatt
     2821 agcatcccag ttttatcttc gtactctgat ggctgcaacg tactccctgg gaaagctttt
     2881 gcaacatgta tagcagcttg aaaggtatca aaatatcgcc caaacattgg cgtttcaaag
     2941 ctaggcgact ctgtcgaaaa atcccccata acttgcgcca aataaccatt atctatctgc
     3001 cattgaacat ctgatcccat agcccactct cccagagtta ctcctaataa ataaccataa
     3061 tatgtattta ccataacgct tcttggccgc accccctgag agcccctcag cgcgtttctg
     3121 cggcctgagg gccgtttccg gcacctttcc ccggcccaaa ccctgaaaaa cgcccctgat
     3181 aatttctgcg cggctgcatg gctatgcaca agagtgaatg gcggccggtt tcgtgaagga
     3241 aataccgtga aagctccgaa agaaacgctt aattgcacag cagcacatga acgtgatcag
     3301 atgaaatctg tctactgccg cacttcttca cgaaaggccc gctgagcgtc agattcgggc
     3361 ctttcttact aaaaagattt caatagaatt ttttagcagg cgatttttat ctataaccat
     3421 acattgagca tagaagaccc aaatttatgt atgactcaat agaaaaaaac aaatcacagc
     3481 catctaaaca ctgagacagc aagccaaaca aaaatcaaag aaataactcc atttttaatc
     3541 aaataatata gttttcgatt cctttttttt agcttctgac ctgcatcacg aatagcatta
     3601 acatatttaa ataaatgatt tatagcacca gtcctgtcat tattatcgtt gggtgatgct
     3661 gccgctgaca ttagcacccc acctatccac ctatttattt tttgtgacca ccgccacttc
     3721 atcggccttt ctatatcaca gaacaaaata atacgtgtct gctcggtatc attctgagcc
     3781 cagtgaacaa aagtttcgtc aaaaattatc acttcaccat catgccaact gcggcgtaca
     3841 ccatcaactt caataaaaca ccgttcatca tttggtgttg ataatcctaa atgataacga
     3901 actgaaccgg cataagggtc acgatgcttt cctaaatggc ttcctgaagg caattcagca
     3961 aacatcgctg ctttaataca gggaatagag ctaaccagtt gggttgttac tgggcaaagc
     4021 cgctccgctg atggatgagc atctccatac cacttgaggt aaaagcgctt ccaaccgcgc
     4081 ttaaaaaatg tattaaatcc tgcatcatta tttttttcag atgatttaat gtgcccttgc
     4141 aaattaacag cttcgtctct tataacctgc cagttatcaa ttaatggttg aagttcagga
     4201 aatgtttctg ggggaaacca tgcctgcttt gacggcagct tcgaaaagcc tgtcataaac
     4261 atatttatcg gagccataaa tgttgaatga tcaaataact gcctggaaaa cttttgtttt
     4321 attttaccgc gagaatgcgc atacaataca ctgataagaa aaacaaaaat aataataagt
     4381 gacagcatgt gtatattcct tgttcacgcc aataataaaa atctcttaaa gataacataa
     4441 tgaatgcact taaattataa tctaaatgct atttatcaat agtataatcg aagcttcacc
     4501 agaacatatc tactacacag gcagagagga tttcagcagg aattatgctg aaatgaatca
     4561 aagggacgga ccgtgatata cccgctgtcg ctgtgatcgt tcgagctcca gcgctttctg
     4621 atgccgcgcc tcccgcacct gctgcagctc gacggcattc agccgctcga caaccggcgc
     4681 agctgatcgc tcaaactctg cacctgcctg ctcaagcccg tgacgcgctc gctcagcgcc
     4741 gcgttctccc gttgcataag
//
