LOCUS       NZ_AP025914             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80376 plasmid pFJMB80376_3,
            complete sequence.
ACCESSION   NZ_AP025914
VERSION     NZ_AP025914.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490888
            Assembly: GCF_023735915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 147x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735915.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:21:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,309
            CDSs (total)                      :: 5,194
            Genes (coding)                    :: 5,099
            CDSs (with protein)               :: 5,099
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 27 of 95
            Pseudo Genes (incomplete)         :: 72 of 95
            Pseudo Genes (internal stop)      :: 12 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025914.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80376"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80376_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-02-03"
     gene            611..754
                     /locus_tag="NGK79_RS26495"
                     /old_locus_tag="FJMB80376_52170"
     CDS             611..754
                     /locus_tag="NGK79_RS26495"
                     /old_locus_tag="FJMB80376_52170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1182..1409
                     /locus_tag="NGK79_RS26500"
                     /old_locus_tag="FJMB80376_52180"
     CDS             1182..1409
                     /locus_tag="NGK79_RS26500"
                     /old_locus_tag="FJMB80376_52180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1776..1961
                     /locus_tag="NGK79_RS26505"
                     /old_locus_tag="FJMB80376_52190"
     CDS             1776..1961
                     /locus_tag="NGK79_RS26505"
                     /old_locus_tag="FJMB80376_52190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2009..2088)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          216..776
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gtagcgggca ggaaaataaa aaaattgcag tcagcccgga acggctgctg aaccggtaca
       61 ggcgggttcc gggcgcagcg ccggaccagt cactcagcgc tagcggagtg tatactggct
      121 aaaatgtaac accgcagtaa cgcatagcat aactgctaca taagacaggc gcagaaaatg
      181 aaagaaaacg cgtcaggaga aaatgtaata caggatatat tccgcttcct cgctcactca
      241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtacgg gcttacgggt ggagcagaaa
      301 tttcctaggg gatgccagct aggggatgcc aggaagatac ttagcgaaga aggcataagg
      361 ccgcgccgaa gccgtttttc cataggctcc gcccccctga cgagcatcac gaaatctgac
      421 gctcaaatca gtggtggcga aacccgatgc ggcttaaaga taccaggcgt ttccccctga
      481 tggctccctc gtgctccccc gcattcctgc ctttcggttt accggtgtca ctccgctgtt
      541 atggccgcgg tttctcattc cacgcctgac actcggttcc gggaaggcag ttcgctccag
      601 gatggactgt atgcacgaac cccccgttca gtccgactgc tgcgccttat ccggtaacta
      661 tcgtcttgag tccaacccgg aaagacacgt aaaaacgcca ctggcagcgg ccactggtaa
      721 ggggtataca agattactgg tgcagagttc ttgaagttga cgcctgactg cggctacact
      781 agaaggacaa atttggtggc agcggtcttg tacaccctgt taccacggtt cagaaacttg
      841 ccggtttgct gaaccttcga aaaaccacct tgccgggtgg ttttttcgtt tacagggcaa
      901 gagattacgc gcgaaaaaaa aggatctcaa gaagatcctt ttataaatta gacatccaac
      961 ctcacaaaga ggaatactta aatcatcttg gtatatgtat cggtcggatt acatcagata
     1021 taagcccgca ctgccccctc cccgccagac accacacttt aaccataaca cctcgcacct
     1081 aactcgatat gcagcccacc atcaagcctc ttttcacata aaaaatgacc tatagttaga
     1141 tggttagtga ccaaaactga tatggataaa aggagctggc tttgaaaaag atttttgttt
     1201 ccgtgttcgc agcggcagtt gcgctttcag ccctcaccgg ctgcacgcgc acaagctatg
     1261 ccatacacac caatgacggg cgcactattg tcagcgacgg caaacctaaa gaatcagact
     1321 caggcctgct tggctataca gacgcgaatg gtgtcaaaca gcagatcaat aaaaccgacg
     1381 ttaaagaagt ctccgaaatt ccgcactgag catcttctcc gaaaaaggcc gctaatgtgg
     1441 cctttttctt tacctggggt tctccgccaa aatcccagca aattgcacca ccaaagctaa
     1501 aagcttccag ggttgtctaa aaagcacaag acattagagg aagtgcggtt tttataatca
     1561 agccccccaa gaatttacca taacatccgt tgcccgcacc gcctcagacg cactcagcgc
     1621 ttttctgacg gaaccatgac caaaaaccac aacaaaccac cccggaaccc gccagaaacg
     1681 ctcctgttaa attttaacgg catgcatgac tatgcaccag aatgacggca tgctcttttc
     1741 acgcaaaaat catcaccaga cggggaaaat caccagtgac cagacaggaa tccgccgccc
     1801 tcaacatggc caaatttatc cgctcacaga cactcctcct cctcgagcgg ctcgaacaga
     1861 tggatctgga tgaggctgcc ggctgctgtg agcacctgca cgatcaggcc gaagcacttt
     1921 acaccatgct gaacgcacag acaggcgagg aagatgcgtg aaaatcggtg aacgggtgcg
     1981 caattcagtg cgcggccgtg aggcgatggc ggggtgtcgg ggcgcagccc tgacctgggt
     2041 atttgtgatg ccggcgcgtg cgcggtatta caaatacaca tcctgtcctg agtggttccc
     2101 cgggaacagg caaaaaaaaa ccggggagct gcccggaagg taagtcaagt tagattagat
     2161 tactttcact catccataac agcatcatgc aacgacgacc atcgtccgtg acggccgcct
     2221 cgtttaagta tggacagaaa tacagaaaat gctcaggacg aaatgtaatg aatgcgaacg
     2281 gattcaagaa attcgagcat gaccgtcctt acggccggtt cagtttcaga caaaatctgc
     2341 cggtatgcat ccagcatcat ggctccggca tccccccggc acgccggagc cagcccgaaa
     2401 caacagacac aaacaggtgt cgctcatcat caagagtcat caggtctccg gaagaaaaac
     2461 caaacatggc ttacctcctg ttaacggcaa cgcagaagaa aggtttacag g
//
