LOCUS       NZ_AP025913             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80376 plasmid pFJMB80376_2,
            complete sequence.
ACCESSION   NZ_AP025913
VERSION     NZ_AP025913.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490888
            Assembly: GCF_023735915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 147x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735915.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:21:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,309
            CDSs (total)                      :: 5,194
            Genes (coding)                    :: 5,099
            CDSs (with protein)               :: 5,099
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 27 of 95
            Pseudo Genes (incomplete)         :: 72 of 95
            Pseudo Genes (internal stop)      :: 12 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025913.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80376"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80376_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-02-03"
     gene            join(4671..4760,1..255)
                     /locus_tag="NGK79_RS26460"
     CDS             join(4671..4760,1..255)
                     /locus_tag="NGK79_RS26460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000764078.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            464..>898
                     /locus_tag="NGK79_RS26465"
                     /pseudo=""
     CDS             464..>898
                     /locus_tag="NGK79_RS26465"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            933..1418
                     /locus_tag="NGK79_RS26470"
                     /old_locus_tag="FJMB80376_52140"
     CDS             933..1418
                     /locus_tag="NGK79_RS26470"
                     /old_locus_tag="FJMB80376_52140"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1422..1637
                     /locus_tag="NGK79_RS26475"
                     /old_locus_tag="FJMB80376_52150"
     CDS             1422..1637
                     /locus_tag="NGK79_RS26475"
                     /old_locus_tag="FJMB80376_52150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1908..2822
                     /gene="lpxO"
                     /locus_tag="NGK79_RS26480"
     CDS             1908..2822
                     /gene="lpxO"
                     /locus_tag="NGK79_RS26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            3222..3683
                     /locus_tag="NGK79_RS26485"
     CDS             3222..3683
                     /locus_tag="NGK79_RS26485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(3938..4048)
                     /locus_tag="NGK79_RS26490"
     CDS             complement(3938..4048)
                     /locus_tag="NGK79_RS26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     Typing          4405..4588
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(3939..4422)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          246..1735
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcagcctgga tgcgccagac gtgcctggac gagaaacccg cccgcgccgg caagcttccc
       61 tcgatctcgc cggcgctgct tcgtcagctt gccggcatgg ggaacaacct taaccagatt
      121 gcccggcagg ttaacgccgg tggtggcagc gggcacgacc gcgtgcaggt cgtcgccgcg
      181 ctgatggcca tagatgccgg actcgagcgg ctgcggcatg ccgtgctgga aaagggtgca
      241 gacgatgatc gttaagtttc acccgcgagg gcgcggcggt ggcgccggtc cggtggatta
      301 tctgctgggg aaagaccgac agcgcgaggg cgccaccgtt ctgcagggga agccggaaga
      361 agtcagggag cttatcgacg cctcacccta cgcaaaaaag tacacatccg gcgtcctgtc
      421 ctttgccgaa aaggatttac cgcccgggca gcgcgaaaag ctgatggcga gctttgaacg
      481 ggttctgatg cccggactcg acaaagacca gtacagcgtg ctgtgggttg agcaccagga
      541 taaggggcgg ctggagctga acttcctgat cccgaacacg gagctgctga ctggcaagcg
      601 tctccagccg tattacgacc gcgccgaccg tccgcgcatt gatgcctggc agaccatcgt
      661 gaacggcagg ctggggctgc atgacccgaa cgcgcctgaa aaccggcggg cgctggtcac
      721 gccgtccgcg ctgccggaaa cgaagcagga agccgctcag gcaattacac ggggtttact
      781 ggccttggcc tcgtccgggg agctgaaaac ccgccaggac gtcactgagg cgctggaaag
      841 cgcaggtttt gaggtggtac gcaccacaaa aagcagcatc agcattgccg acccggacgg
      901 ggggcgaaac atccgactga agggagccat ctatgaacag tcttttaacg ctggcgaagg
      961 acttagagca gaaatcgaaa gcgcagcagc agagtaccgg cgagatgctg aaagccgcat
     1021 tcagcgagca cgagaagtct gtcagagcgg aactgagcga aagcgagaag agaatcagcg
     1081 ccgccatcct cgaccacgac cggaagctgt cctcagccat gagccagcgt acgaaaggga
     1141 tgctgcgcat ggtcagccag acgtggctga ccatcgtcct ggtctccgtg ctgctgatcg
     1201 cctcgaacgc ggccattctg tggtggcaga gtcagcagat actcgacaat tacgtgagca
     1261 tccgggagca gaagagcacg caggcgatgc tgtccgagcg gaacagcggc gtgcagctct
     1321 cgacctgcgg cgagcagaga cgccgctgcg tgagggtgaa cccggaagcg ggacggttcg
     1381 gagaggattc gagctggatg atactggcgg ggaaatagca catgacagag ctggaaaaac
     1441 agttgctgag cgcattagag cagctacagc aggactactc gaaaaggctg gacgagtggg
     1501 agagcgcctt cgcggaatgg cggacgatgt ctggtcttat gcaacgggag aacgcggcgc
     1561 tgagcgagcg cgtcacgggc ttgagcaggc aggtgcagag tttgagcgat cagctgcgcc
     1621 ggttgtcgag cggctgaatg ccgtcgagct gcagcaggtg cgggaggcgc ggcatcagaa
     1681 agcgctggag ctcgaacgat cacagcgaca gcgggtatat cacggtccgt ccctttgatt
     1741 catttcagca taattcctgc tgaaatcctc tctgcctgtg tagtagatat gttctggtga
     1801 agcttcgatt atactattga taaatagcat ttagattata atttaagtgc attcattatg
     1861 ttatctttaa gagattttta ttattggcgt gaacaaggaa tatacacatg ctgtcactta
     1921 ttattatttt tgtttttctt atcagtgtat tgtatgcgca ttctcgcggt aaaataaaac
     1981 aaaagttttc caggcagtta tttgatcatt caacatttat ggctccgata aatatgttta
     2041 tgacaggctt ttcgaagctg ccgtcaaagc aggcatggtt tcccccagaa acatttcctg
     2101 aacttcaacc attaattgat aactggcagg ttataagaga cgaagctgtt aatttgcaag
     2161 ggcacattaa atcatctgaa aaaaataatg atgcaggatt taatacattt tttaagcgcg
     2221 gttggaagcg cttttacctc aagtggtatg gagatgctca tccatcagcg gagcggcttt
     2281 gcccagtaac aacccaactg gttagctcta ttccctgtat taaagcagcg atgtttgctg
     2341 aattgccttc aggaagccat ttaggaaagc atcgtgaccc ttatgccggt tcagttcgtt
     2401 atcatttagg attatcaaca ccaaatgatg aacggtgttt tattgaagtt gatggtgtac
     2461 gccgcagttg gcatgatggt gaagtgataa tttttgacga aacttttgtt cactgggctc
     2521 agaatgatac cgagcagaca cgtattattt tgttctgtga tatagaaagg ccgatgaagt
     2581 ggcggtggtc acaaaaaata aataggtgga taggtggggt gctaatgtca gcggcagcat
     2641 cacccaacga taataatgac aggactggtg ctataaatca tttatttaaa tatgttaatg
     2701 ctattcgtga tgcaggtcag aagctaaaaa aaaggaatcg aaaactatat tatttgatta
     2761 aaaatggagt tatttctttg atttttgttt ggcttgctgt ctcagtgttt agatggctgt
     2821 gatttgtttt tttctattga gtcatacata aatttgggtc ttctatgctc aatgtatggt
     2881 tatagataaa aatcgcctgc taaaaaattc tattgaaatc tttttagtaa gaaaggcccg
     2941 aatctgacgc tcagcgggcc tttcgtgaag aagtgcggca gtagacagat ttcatctgat
     3001 cacgttcatg tgctgctgtg caattaagcg tttctttcgg agctttcacg gtatttcctt
     3061 cacgaaaccg gccgccattc actcttgtgc atagccatgc agccgcgcag aaattatcag
     3121 gggcgttttt cagggtttgg gccggggaaa ggtgccggaa acggccctca ggccgcagaa
     3181 acgcgctgag gggctctcag ggggtgcggc caagaagcgt tatggtaaat acatattatg
     3241 gttatttatt aggagtaact ctgggagagt gggctatggg atcagatgtt caatggcaga
     3301 tagataatgg ttatttggcg caagttatgg gggatttttc gacagagtcg cctagctttg
     3361 aaacgccaat gtttgggcga tattttgata cctttcaagc tgctatacat gttgcaaaag
     3421 ctttcccagg gagtacgttg cagccatcag agtacgaaga taaaactggg atgctaatac
     3481 gtcattatta tgtggattgt catccgtatt atgatcgact ccctcctgaa aaaagcgata
     3541 attttcagta tgatgagtac ccttctgcta ttcatgtggc tgcgtgtatt gatcaagcta
     3601 gcaaagaact cgacgaacta ttgtttggtg gtggatatgg ggaaaatggt gaggggcatg
     3661 caagcccgat aattttatat tagtgttagg ggcggaggga tattatccaa tttgtaaaat
     3721 gatcttcttg agatcgtttt gctctgcgcg taatctcttg ctctgaaaac gaaaaaacca
     3781 cctggggagg tggtttttcg aaggttcagt aagttgggga acttctgaac cgtggtaaca
     3841 gtcttgtgcg agacgtgtca ccaaatctgt ccttccagtg tagccgtagt ccggccacca
     3901 cttcaagaac tctcgataca tctctcgcac atcctgctta ccagtggcta ccgccaatgg
     3961 cgattttgtc gtgtctttcc gggttggact caagttgata gttaccggaa tgggcgtggt
     4021 agtcggactg aacggggggg tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     4081 aactgagtgt caggcgtgga atgagacaaa cgcggccaca taacagcgga atgacaccgg
     4141 taaaccgaaa ggcaggaaca ggagagcgca cgagggagct accaggggga aacgcctggg
     4201 atctttatag tcctgtcggg tttcgccgcc actgatttga gcgtcatatt tcgtgatgct
     4261 tgtcaggggg gcggagccta tggaaaaacg gcaacgccgc atccttttcc cagcccttcc
     4321 tgatttgagc gccatctctt tatcccgcca ttcgtgagcc ggtaacgccc gccgcagtct
     4381 cacggcagga gcgtagcgac cgagtgagcg aggaggcgtc atttcatcgt ccccagcatt
     4441 tcgcacttac gccccacaga cgcatttttt acctgccaca tgaagcactt ccctgaattc
     4501 ccacttcgat ttaccatatt aagccagtat acactccgct agcgctacgt gactggttca
     4561 gggctgcgcc ccgaaacccg ctaaaaccac tgacgcgcct gcggcttgtc caacaccgcg
     4621 ccgaccggga aaagatttcc cgcccgtccc ggcgttatca ggaggccgga ttggcagaca
     4681 aacgcagcaa aatgctcacg atgtgggtga ccgaggatga gcaccggcgt ctgctggagc
     4741 gctgcgacgg taaacagctt
//
