LOCUS       NZ_AP025910             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80375 plasmid pFJMB80375_3,
            complete sequence.
ACCESSION   NZ_AP025910
VERSION     NZ_AP025910.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490887
            Assembly: GCF_023735895.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 146x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735895.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:11:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,260
            CDSs (total)                      :: 5,145
            Genes (coding)                    :: 5,054
            CDSs (with protein)               :: 5,054
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 26 of 91
            Pseudo Genes (incomplete)         :: 69 of 91
            Pseudo Genes (internal stop)      :: 12 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025910.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80375"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80375_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-11-01"
     gene            complement(129..272)
                     /locus_tag="NGK01_RS26245"
                     /old_locus_tag="FJMB80375_51610"
     CDS             complement(129..272)
                     /locus_tag="NGK01_RS26245"
                     /old_locus_tag="FJMB80375_51610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(1433..1618)
                     /locus_tag="NGK01_RS26250"
                     /old_locus_tag="FJMB80375_51630"
     CDS             complement(1433..1618)
                     /locus_tag="NGK01_RS26250"
                     /old_locus_tag="FJMB80375_51630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1985..2212)
                     /locus_tag="NGK01_RS26255"
                     /old_locus_tag="FJMB80375_51640"
     CDS             complement(1985..2212)
                     /locus_tag="NGK01_RS26255"
                     /old_locus_tag="FJMB80375_51640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1307..1386
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(108..668)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 aaccacccgg caaggtggtt tttcgaaggt tcagcaaacc ggcaagtttc tgaaccgtgg
       61 taacagggtg tacaagaccg ctgccaccaa atttgtcctt ctagtgtagc cgcagtcagg
      121 cgtcaacttc aagaactctg caccagtaat cttgtatacc ccttaccagt ggccgctgcc
      181 agtggcgttt ttacgtgtct ttccgggttg gactcaagac gatagttacc ggataaggcg
      241 cagcagtcgg actgaacggg gggttcgtgc atacagtcca tcctggagcg aactgccttc
      301 ccggaaccga gtgtcaggcg tggaatgaga aaccgcggcc ataacagcgg agtgacaccg
      361 gtaaaccgaa aggcaggaat gcgggggagc acgagggagc catcaggggg aaacgcctgg
      421 tatctttaag ccgcatcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
      481 cgtcaggggg gcggagccta tggaaaaacg gcttcggcgc ggccttatgc cttcttcgct
      541 aagtatcttc ctggcatccc ctagctggca tcccctagga aatttctgct ccacccgtaa
      601 gcccgtaccg ctcgccgcag ccgaacgacc gagcgcagcg agtgagtgag cgaggaagcg
      661 gaatatatcc tgtattacat tttctcctga cgcgttttct ttcattttct gcgcctgtct
      721 tatgtagcag ttatgctatg cgttactgcg gtgttacatt ttagccagta tacactccgc
      781 tagcgctgag tgactggtcc ggcgctgcgc ccggaacccg cctgtaccgg ttcagcagcc
      841 gttccgggct gactgcaatt tttttatttt cctgcccgct accctgtaaa cctttcttct
      901 gcgttgccgt taacaggagg taagccatgt ttggtttttc ttccggagac ctgatgactc
      961 ttgatgatga gcgacacctg tttgtgtctg ttgtttcggg ctggctccgg cgtgccgggg
     1021 ggatgccgga gccatgatgc tggatgcata ccggcagatt ttgtctgaaa ctgaaccggc
     1081 cgtaaggacg gtcatgctcg aatttcttga atccgttcgc attcattaca tttcgtcctg
     1141 agcattttct gtatttctgt ccatacttaa acgaggcggc cgtcacggac gatggtcgtc
     1201 gttgcatgat gctgttatgg atgagtgaaa gtaatctaat ctaacttgac ttaccttccg
     1261 ggcagctccc cggttttttt ttgcctgttc ccggggaacc actcaggaca ggatgtgtat
     1321 ttgtaatacc gcgcacgcgc cggcatcaca aatacccagg tcagggctgc gccccgacac
     1381 cccgccatcg cctcacggcc gcgcactgaa ttgcgcaccc gttcaccgat tttcacgcat
     1441 cttcctcgcc tgtctgtgcg ttcagcatgg tgtaaagtgc ttcggcctga tcgtgcaggt
     1501 gctcacagca gccggcagcc tcatccagat ccatctgttc gagccgctcg aggaggagga
     1561 gtgtctgtga gcggataaat ttggccatgt tgagggcggc ggattcctgt ctggtcactg
     1621 gtgattttcc ccgtctggtg atgatttttg cgtgaaaaga gcatgccgtc attctggtgc
     1681 atagtcatgc atgccgttaa aatttaacag gagcgtttct ggcgggttcc ggggtggttt
     1741 gttgtggttt ttggtcatgg ttccgtcaga aaagcgctga gtgcgtctga ggcggtgcgg
     1801 gcaacggatg ttatggtaaa ttcttggggg gcttgattat aaaaaccgca cttcctctaa
     1861 tgtcttgtgc tttttagaca accctggaag cttttagctt tggtggtgca atttgctggg
     1921 attttggcgg agaaccccag gtaaagaaaa aggccacatt agcggccttt ttcggagaag
     1981 atgctcagtg cggaatttcg gagacttctt taacgtcggt tttattgatc tgctgtttga
     2041 caccattcgc gtctgtatag ccaagcaggc ctgagtctga ttctttaggt ttgccgtcgc
     2101 tgacaatagt gcgcccgtca ttggtgtgta tggcatagct tgtgcgcgtg cagccggtga
     2161 gggctgaaag cgcaactgcc gctgcgaaca cggaaacaaa aatctttttc aaagccagct
     2221 ccttttatcc atatcagttt tggtcactaa ccatctaact ataggtcatt ttttatgtga
     2281 aaagaggctt gatggtgggc tgcatatcga gttaggtgcg aggtgttatg gttaaagtgt
     2341 ggtgtctggc ggggaggggg cagtgcgggc ttatatctga tgtaatccga ccgatacata
     2401 taccaagatg atttaagtat tcctctttgt gaggttggat gtctaattta taaaaggatc
     2461 ttcttgagat cctttttttt cgcgcgtaat ctcttgccct gtaaacgaaa a
//
