LOCUS       NZ_AP025909             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80375 plasmid pFJMB80375_2,
            complete sequence.
ACCESSION   NZ_AP025909
VERSION     NZ_AP025909.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490887
            Assembly: GCF_023735895.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 146x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735895.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:11:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,260
            CDSs (total)                      :: 5,145
            Genes (coding)                    :: 5,054
            CDSs (with protein)               :: 5,054
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 26 of 91
            Pseudo Genes (incomplete)         :: 69 of 91
            Pseudo Genes (internal stop)      :: 12 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025909.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80375"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80375_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-11-01"
     gene            complement(join(4713..4760,1..276))
                     /locus_tag="NGK01_RS26210"
     CDS             complement(join(4713..4760,1..276))
                     /locus_tag="NGK01_RS26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            920..1030
                     /locus_tag="NGK01_RS26215"
     CDS             920..1030
                     /locus_tag="NGK01_RS26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(1285..1746)
                     /locus_tag="NGK01_RS26220"
     CDS             complement(1285..1746)
                     /locus_tag="NGK01_RS26220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(2146..3060)
                     /gene="lpxO"
                     /locus_tag="NGK01_RS26225"
     CDS             complement(2146..3060)
                     /gene="lpxO"
                     /locus_tag="NGK01_RS26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(3331..3546)
                     /locus_tag="NGK01_RS26230"
                     /old_locus_tag="FJMB80375_51590"
     CDS             complement(3331..3546)
                     /locus_tag="NGK01_RS26230"
                     /old_locus_tag="FJMB80375_51590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3550..4035)
                     /locus_tag="NGK01_RS26235"
                     /old_locus_tag="FJMB80375_51600"
     CDS             complement(3550..4035)
                     /locus_tag="NGK01_RS26235"
                     /old_locus_tag="FJMB80375_51600"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            4286..4531
                     /locus_tag="NGK01_RS26330"
     CDS             4286..4531
                     /locus_tag="NGK01_RS26330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023343077.1"
                     /translation="MQPQPAVHDGLPGINARTVGAVVIRLETLASQQLRVRDQEVQLQP
                     PLILVLNPQHAVLVFVESGHQNPFKARHQLFALPGR"
                     /name="hypothetical protein"
     Typing          complement(378..564)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          544..1030
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(2..276)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.913"
                     /coverage="86.0"
                     /evalue="1.4999999999999998e-54"
     Typing          complement(3234..4723)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcgagtccg gcatctatgg ccatcagcgc ggcgacgacc tgcacgcggt cgtgcccgct
       61 gccaccaccg gcgttaacct gccgggcaat ctggttaagg ttgttcccca tgccggcaag
      121 ctgacgaagc agcgccggcg agatcgaggg aagcttgccg gcgcgggcgg gtttctcgtc
      181 caggcacgtc tggcgcatcc aggctgcaag ctgtttaccg tcgcagcgct ccagcagacg
      241 ccggtgctca tcctcggtca cccacatcgt gagcattttg ctgcgtttgt ctgccaatcc
      301 ggcctcctga taacgccggg acgggcggga aatcttttcc cggtcggcgc ggtgttggac
      361 aagccgcagg cgcgtcagtg gttttagcgg gtttcggggc gcagccctga accagtcacg
      421 tagcgctagc ggagtgtata ctggcttaat atggtaaatc gaagtgggaa ttcagggaag
      481 tgcttcatgt ggcaggtaaa aaatgcgtct gtggggcgta agtgcgaaat gctggggacg
      541 atgaaatgac gcctcctcgc tcactcggtc gctacgctcc tgccgtgaga ctgcggcggg
      601 cgttaccggc tcacgaatgg cgggataaag agatggcgct caaatcagga agggctggga
      661 aaaggatgcg gcgttgccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
      721 atgacgctca aatcagtggc ggcgaaaccc gacaggacta taaagatccc aggcgtttcc
      781 ccctggtagc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
      841 gctgttatgt ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
      901 tcgctccaag ctggactgta tgcacgaccc ccccgttcag tccgactacc acgcccattc
      961 cggtaactat caacttgagt ccaacccgga aagacacgac aaaatcgcca ttggcggtag
     1021 ccactggtaa gcaggatgtg cgagagatgt atcgagagtt cttgaagtgg tggccggact
     1081 acggctacac tggaaggaca gatttggtga cacgtctcgc acaagactgt taccacggtt
     1141 cagaagttcc ccaacttact gaaccttcga aaaaccacct ccccaggtgg ttttttcgtt
     1201 ttcagagcaa gagattacgc gcagagcaaa acgatctcaa gaagatcatt ttacaaattg
     1261 gataatatcc ctccgcccct aacactaata taaaattatc gggcttgcat gcccctcacc
     1321 attttcccca tatccaccac caaacaatag ttcgtcgagt tctttgctag cttgatcaat
     1381 acacgcagcc acatgaatag cagaagggta ctcatcatac tgaaaattat cgcttttttc
     1441 aggagggagt cgatcataat acggatgaca atccacataa taatgacgta ttagcatccc
     1501 agttttatct tcgtactctg atggctgcaa cgtactccct gggaaagctt ttgcaacatg
     1561 tatagcagct tgaaaggtat caaaatatcg cccaaacatt ggcgtttcaa agctaggcga
     1621 ctctgtcgaa aaatccccca taacttgcgc caaataacca ttatctatct gccattgaac
     1681 atctgatccc atagcccact ctcccagagt tactcctaat aaataaccat aatatgtatt
     1741 taccataacg cttcttggcc gcaccccctg agagcccctc agcgcgtttc tgcggcctga
     1801 gggccgtttc cggcaccttt ccccggccca aaccctgaaa aacgcccctg ataatttctg
     1861 cgcggctgca tggctatgca caagagtgaa tggcggccgg tttcgtgaag gaaataccgt
     1921 gaaagctccg aaagaaacgc ttaattgcac agcagcacat gaacgtgatc agatgaaatc
     1981 tgtctactgc cgcacttctt cacgaaaggc ccgctgagcg tcagattcgg gcctttctta
     2041 ctaaaaagat ttcaatagaa ttttttagca ggcgattttt atctataacc atacattgag
     2101 catagaagac ccaaatttat gtatgactca atagaaaaaa acaaatcaca gccatctaaa
     2161 cactgagaca gcaagccaaa caaaaatcaa agaaataact ccatttttaa tcaaataata
     2221 tagttttcga ttcctttttt ttagcttctg acctgcatca cgaatagcat taacatattt
     2281 aaataaatga tttatagcac cagtcctgtc attattatcg ttgggtgatg ctgccgctga
     2341 cattagcacc ccacctatcc acctatttat tttttgtgac caccgccact tcatcggcct
     2401 ttctatatca cagaacaaaa taatacgtgt ctgctcggta tcattctgag cccagtgaac
     2461 aaaagtttcg tcaaaaatta tcacttcacc atcatgccaa ctgcggcgta caccatcaac
     2521 ttcaataaaa caccgttcat catttggtgt tgataatcct aaatgataac gaactgaacc
     2581 ggcataaggg tcacgatgct ttcctaaatg gcttcctgaa ggcaattcag caaacatcgc
     2641 tgctttaata cagggaatag agctaaccag ttgggttgtt actgggcaaa gccgctccgc
     2701 tgatggatga gcatctccat accacttgag gtaaaagcgc ttccaaccgc gcttaaaaaa
     2761 tgtattaaat cctgcatcat tatttttttc agatgattta atgtgccctt gcaaattaac
     2821 agcttcgtct cttataacct gccagttatc aattaatggt tgaagttcag gaaatgtttc
     2881 tgggggaaac catgcctgct ttgacggcag cttcgaaaag cctgtcataa acatatttat
     2941 cggagccata aatgttgaat gatcaaataa ctgcctggaa aacttttgtt ttattttacc
     3001 gcgagaatgc gcatacaata cactgataag aaaaacaaaa ataataataa gtgacagcat
     3061 gtgtatattc cttgttcacg ccaataataa aaatctctta aagataacat aatgaatgca
     3121 cttaaattat aatctaaatg ctatttatca atagtataat cgaagcttca ccagaacata
     3181 tctactacac aggcagagag gatttcagca ggaattatgc tgaaatgaat caaagggacg
     3241 gaccgtgata tacccgctgt cgctgtgatc gttcgagctc cagcgctttc tgatgccgcg
     3301 cctcccgcac ctgctgcagc tcgacggcat tcagccgctc gacaaccggc gcagctgatc
     3361 gctcaaactc tgcacctgcc tgctcaagcc cgtgacgcgc tcgctcagcg ccgcgttctc
     3421 ccgttgcata agaccagaca tcgtccgcca ttccgcgaag gcgctctccc actcgtccag
     3481 ccttttcgag tagtcctgct gtagctgctc taatgcgctc agcaactgtt tttccagctc
     3541 tgtcatgtgc tatttccccg ccagtatcat ccagctcgaa tcctctccga accgtcccgc
     3601 ttccgggttc accctcacgc agcggcgtct ctgctcgccg caggtcgaga gctgcacgcc
     3661 gctgttccgc tcggacagca tcgcctgcgt gctcttctgc tcccggatgc tcacgtaatt
     3721 gtcgagtatc tgctgactct gccaccacag aatggccgcg ttcgaggcga tcagcagcac
     3781 ggagaccagg acgatggtca gccacgtctg gctgaccatg cgcagcatcc ctttcgtacg
     3841 ctggctcatg gctgaggaca gcttccggtc gtggtcgagg atggcggcgc tgattctctt
     3901 ctcgctttcg ctcagttccg ctctgacaga cttctcgtgc tcgctgaatg cggctttcag
     3961 catctcgccg gtactctgct gctgcgcttt cgatttctgc tctaagtcct tcgccagcgt
     4021 taaaagactg ttcatagatg gctcccttca gtcggatgtt tcgccccccg tccgggtcgg
     4081 caatgctgat gctgcttttt gtggtgcgta ccacctcaaa acctgcgctt tccagcgcct
     4141 cagtgacgtc ctggcgggtt ttcagctccc cggacgaggc caaggccagt aaaccccgtg
     4201 taattgcctg agcggcttcc tgcttcgttt ccggcagcgc ggacggcgtg accagcgccc
     4261 gccggttttc aggcgcgttc gggtcatgca gccccagcct gccgttcacg atggtctgcc
     4321 aggcatcaat gcgcggacgg tcggcgcggt cgtaatacgg ctggagacgc ttgccagtca
     4381 gcagctccgt gttcgggatc aggaagttca gctccagccg ccccttatcc tggtgctcaa
     4441 cccacagcac gctgtactgg tctttgtcga gtccgggcat cagaacccgt tcaaagctcg
     4501 ccatcagctt ttcgcgctgc ccgggcggta aatccttttc ggcaaaggac aggacgccgg
     4561 atgtgtactt ttttgcgtag ggtgaggcgt cgataagctc cctgacttct tccggcttcc
     4621 cctgcagaac ggtggcgccc tcgcgctgtc ggtctttccc cagcagataa tccaccggac
     4681 cggcgccacc gccgcgccct cgcgggtgaa acttaacgat catcgtctgc acccttttcc
     4741 agcacggcat gccgcagccg
//
