LOCUS       NZ_AP025906             2511 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80374 plasmid pFJMB80374_3,
            complete sequence.
ACCESSION   NZ_AP025906
VERSION     NZ_AP025906.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490886
            Assembly: GCF_023735875.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 154x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735875.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:29:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 5,091
            CDSs (with protein)               :: 5,091
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 27 of 100
            Pseudo Genes (incomplete)         :: 77 of 100
            Pseudo Genes (internal stop)      :: 12 of 100
            Pseudo Genes (multiple problems)  :: 14 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025906.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80374"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80374_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-04-24"
     gene            join(2394..2511,1..68)
                     /locus_tag="NGK42_RS26480"
     CDS             join(2394..2511,1..68)
                     /locus_tag="NGK42_RS26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1229..1372
                     /locus_tag="NGK42_RS26485"
                     /old_locus_tag="FJMB80374_52130"
     CDS             1229..1372
                     /locus_tag="NGK42_RS26485"
                     /old_locus_tag="FJMB80374_52130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1800..2027
                     /locus_tag="NGK42_RS26490"
                     /old_locus_tag="FJMB80374_52140"
     CDS             1800..2027
                     /locus_tag="NGK42_RS26490"
                     /old_locus_tag="FJMB80374_52140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(116..195)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          834..1394
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 acctgcacga tcaggccgaa gcactttaca ccatgctgaa cgcacagaca ggcgaggaag
       61 atgcgtgaaa atcggtgaac gggtgcgcaa ttcagtgcgc ggccgtgagg cgatggcggg
      121 gtgtcggggc gcagccctga cctgggtatt tgtgatgccg gcgcgtgcgc ggtattacaa
      181 atacacatcc tgtcctgagt ggttccccgg gaacaggcaa aaaaaaaccg gggagctgcc
      241 cggaaggtaa gtcaagttag attagattac tttcactcat ccataacagc atcatgcaac
      301 gacgaccatc gtccgtgacg gccgcctcgt ttaagtatgg acagaaatac agaaaatgct
      361 caggacgaaa tgtaatgaat gcgaacggat tcaagaaatt cgagcatgac cgtccttacg
      421 gccggttcag tttcagacaa aatctgccgg tatgcatcca gcatcatggc tccggcatcc
      481 ccccggcacg ccggagccag cccgaaacaa cagacacaaa caggtgtcgc tcatcatcaa
      541 gagtcatcag gtctccggaa gaaaaaccaa acatggctta cctcctgtta acggcaacgc
      601 agaagaaagg tttacagggt agcgggcagg aaaataaaaa aattgcagtc agcccggaac
      661 ggctgctgaa ccggtacagg cgggttccgg gcgcagcgcc ggaccagtca ctcagcgcta
      721 gcggagtgta tactggctaa aatgtaacac cgcagtaacg catagcataa ctgctacata
      781 agacaggcgc agaaaatgaa agaaaacgcg tcaggagaaa atgtaataca ggatatattc
      841 cgcttcctcg ctcactcact cgctgcgctc ggtcgttcgg ctgcggcgag cggtacgggc
      901 ttacgggtgg agcagaaatt tcctagggga tgccagctag gggatgccag gaagatactt
      961 agcgaagaag gcataaggcc gcgccgaagc cgtttttcca taggctccgc ccccctgacg
     1021 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgatgcgg cttaaagata
     1081 ccaggcgttt ccccctgatg gctccctcgt gctcccccgc attcctgcct ttcggtttac
     1141 cggtgtcact ccgctgttat ggccgcggtt tctcattcca cgcctgacac tcggttccgg
     1201 gaaggcagtt cgctccagga tggactgtat gcacgaaccc cccgttcagt ccgactgctg
     1261 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacacgtaa aaacgccact
     1321 ggcagcggcc actggtaagg ggtatacaag attactggtg cagagttctt gaagttgacg
     1381 cctgactgcg gctacactag aaggacaaat ttggtggcag cggtcttgta caccctgtta
     1441 ccacggttca gaaacttgcc ggtttgctga accttcgaaa aaccaccttg ccgggtggtt
     1501 ttttcgttta cagggcaaga gattacgcgc gaaaaaaaag gatctcaaga agatcctttt
     1561 ataaattaga catccaacct cacaaagagg aatacttaaa tcatcttggt atatgtatcg
     1621 gtcggattac atcagatata agcccgcact gccccctccc cgccagacac cacactttaa
     1681 ccataacacc tcgcacctaa ctcgatatgc agcccaccat caagcctctt ttcacataaa
     1741 aaatgaccta tagttagatg gttagtgacc aaaactgata tggataaaag gagctggctt
     1801 tgaaaaagat ttttgtttcc gtgttcgcag cggcagttgc gctttcagcc ctcaccggct
     1861 gcacgcgcac aagctatgcc atacacacca atgacgggcg cactattgtc agcgacggca
     1921 aacctaaaga atcagactca ggcctgcttg gctatacaga cgcgaatggt gtcaaacagc
     1981 agatcaataa aaccgacgtt aaagaagtct ccgaaattcc gcactgagca tcttctccga
     2041 aaaaggccgc taatgtggcc tttttcttta cctggggttc tccgccaaaa tcccagcaaa
     2101 ttgcaccacc aaagctaaaa gcttccaggg ttgtctaaaa agcacaagac attagaggaa
     2161 gtgcggtttt tataatcaag ccccccaaga atttaccata acatccgttg cccgcaccgc
     2221 ctcagacgca ctcagcgctt ttctgacgga accatgacca aaaaccacaa caaaccaccc
     2281 cggaacccgc cagaaacgct cctgttaaat tttaacggca tgcatgacta tgcaccagaa
     2341 tgacggcatg ctcttttcac gcaaaaatca tcaccagacg gggaaaatca ccagtgacca
     2401 gacaggaatc cgccgccctc aacatggcca aatttatccg ctcacagaca ctcctcctcc
     2461 tcgagcggct cgaacagatg gatctggatg aggctgccgg ctgctgtgag c
//
