LOCUS       NZ_AP025905             4760 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80374 plasmid pFJMB80374_2,
            complete sequence.
ACCESSION   NZ_AP025905
VERSION     NZ_AP025905.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490886
            Assembly: GCF_023735875.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 154x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735875.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:29:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 5,091
            CDSs (with protein)               :: 5,091
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 27 of 100
            Pseudo Genes (incomplete)         :: 77 of 100
            Pseudo Genes (internal stop)      :: 12 of 100
            Pseudo Genes (multiple problems)  :: 14 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025905.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80374"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80374_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-04-24"
     gene            complement(3..488)
                     /locus_tag="NGK42_RS26445"
                     /old_locus_tag="FJMB80374_52090"
     CDS             complement(3..488)
                     /locus_tag="NGK42_RS26445"
                     /old_locus_tag="FJMB80374_52090"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<523..1227)
                     /locus_tag="NGK42_RS26565"
                     /pseudo=""
     CDS             complement(<523..1227)
                     /locus_tag="NGK42_RS26565"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1166..1489)
                     /locus_tag="NGK42_RS26455"
                     /old_locus_tag="FJMB80374_52100"
     CDS             complement(1166..1489)
                     /locus_tag="NGK42_RS26455"
                     /old_locus_tag="FJMB80374_52100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2133..2243
                     /locus_tag="NGK42_RS26460"
     CDS             2133..2243
                     /locus_tag="NGK42_RS26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(2498..2959)
                     /locus_tag="NGK42_RS26465"
     CDS             complement(2498..2959)
                     /locus_tag="NGK42_RS26465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(3359..4273)
                     /gene="lpxO"
                     /locus_tag="NGK42_RS26470"
     CDS             complement(3359..4273)
                     /gene="lpxO"
                     /locus_tag="NGK42_RS26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(4544..4759)
                     /locus_tag="NGK42_RS26475"
                     /old_locus_tag="FJMB80374_52120"
     CDS             complement(4544..4759)
                     /locus_tag="NGK42_RS26475"
                     /old_locus_tag="FJMB80374_52120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1591..1777)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          1757..2243
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(2..1176)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.622"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgctatttcc ccgccagtat catccagctc gaatcctctc cgaaccgtcc cgcttccggg
       61 ttcaccctca cgcagcggcg tctctgctcg ccgcaggtcg agagctgcac gccgctgttc
      121 cgctcggaca gcatcgcctg cgtgctcttc tgctcccgga tgctcacgta attgtcgagt
      181 atctgctgac tctgccacca cagaatggcc gcgttcgagg cgatcagcag cacggagacc
      241 aggacgatgg tcagccacgt ctggctgacc atgcgcagca tccctttcgt acgctggctc
      301 atggctgagg acagcttccg gtcgtggtcg aggatggcgg cgctgattct cttctcgctt
      361 tcgctcagtt ccgctctgac agacttctcg tgctcgctga atgcggcttt cagcatctcg
      421 ccggtactct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag cgttaaaaga
      481 ctgttcatag atggctccct tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct
      541 gatgctgctt tttgtggtgc gtaccacctc aaaacctgcg ctttccagcg cctcagtgac
      601 gtcctggcgg gttttcagct ccccggacga ggccaaggcc agtaaacccc gtgtaattgc
      661 ctgagcggct tcctgcttcg tttccggcag cgcggacggc gtgaccagcg cccgccggtt
      721 ttcaggcgcg ttcgggtcat gcagccccag cctgccgttc acgatggtct gccaggcatc
      781 aatgcgcgga cggtcggcgc ggtcgtaata cggctggaga cgcttgccag tcagcagctc
      841 cgtgttcggg atcaggaagt tcagctccag ccgcccctta tcctggtgct caacccacag
      901 cacgctgtac tggtctttgt cgagtccggg catcagaacc cgttcaaagc tcgccatcag
      961 cttttcgcgc tgcccgggcg gtaaatcctt ttcggcaaag gacaggacgc cggatgtgta
     1021 cttttttgcg tagggtgagg cgtcgataag ctccctgact tcttccggct tcccctgcag
     1081 aacggtggcg ccctcgcgct gtcggtcttt ccccagcaga taatccaccg gaccggcgcc
     1141 accgccgcgc cctcgcgggt gaaacttaac gatcatcgtc tgcacccttt tccagcacgg
     1201 catgccgcag ccgctcgagt ccggcatcta tggccatcag cgcggcgacg acctgcacgc
     1261 ggtcgtgccc gctgccacca ccggcgttaa cctgccgggc aatctggtta aggttgttcc
     1321 ccatgccggc aagctgacga agcagcgccg gcgagatcga gggaagcttg ccggcgcggg
     1381 cgggtttctc gtccaggcac gtctggcgca tccaggctgc aagctgttta ccgtcgcagc
     1441 gctccagcag acgccggtgc tcatcctcgg tcacccacat cgtgagcatt ttgctgcgtt
     1501 tgtctgccaa tccggcctcc tgataacgcc gggacgggcg ggaaatcttt tcccggtcgg
     1561 cgcggtgttg gacaagccgc aggcgcgtca gtggttttag cgggtttcgg ggcgcagccc
     1621 tgaaccagtc acgtagcgct agcggagtgt atactggctt aatatggtaa atcgaagtgg
     1681 gaattcaggg aagtgcttca tgtggcaggt aaaaaatgcg tctgtggggc gtaagtgcga
     1741 aatgctgggg acgatgaaat gacgcctcct cgctcactcg gtcgctacgc tcctgccgtg
     1801 agactgcggc gggcgttacc ggctcacgaa tggcgggata aagagatggc gctcaaatca
     1861 ggaagggctg ggaaaaggat gcggcgttgc cgtttttcca taggctccgc ccccctgaca
     1921 agcatcacga aatatgacgc tcaaatcagt ggcggcgaaa cccgacagga ctataaagat
     1981 cccaggcgtt tccccctggt agctccctcg tgcgctctcc tgttcctgcc tttcggttta
     2041 ccggtgtcat tccgctgtta tgtggccgcg tttgtctcat tccacgcctg acactcagtt
     2101 ccgggtaggc agttcgctcc aagctggact gtatgcacga cccccccgtt cagtccgact
     2161 accacgccca ttccggtaac tatcaacttg agtccaaccc ggaaagacac gacaaaatcg
     2221 ccattggcgg tagccactgg taagcaggat gtgcgagaga tgtatcgaga gttcttgaag
     2281 tggtggccgg actacggcta cactggaagg acagatttgg tgacacgtct cgcacaagac
     2341 tgttaccacg gttcagaagt tccccaactt actgaacctt cgaaaaacca cctccccagg
     2401 tggttttttc gttttcagag caagagatta cgcgcagagc aaaacgatct caagaagatc
     2461 attttacaaa ttggataata tccctccgcc cctaacacta atataaaatt atcgggcttg
     2521 catgcccctc accattttcc ccatatccac caccaaacaa tagttcgtcg agttctttgc
     2581 tagcttgatc aatacacgca gccacatgaa tagcagaagg gtactcatca tactgaaaat
     2641 tatcgctttt ttcaggaggg agtcgatcat aatacggatg acaatccaca taataatgac
     2701 gtattagcat cccagtttta tcttcgtact ctgatggctg caacgtactc cctgggaaag
     2761 cttttgcaac atgtatagca gcttgaaagg tatcaaaata tcgcccaaac attggcgttt
     2821 caaagctagg cgactctgtc gaaaaatccc ccataacttg cgccaaataa ccattatcta
     2881 tctgccattg aacatctgat cccatagccc actctcccag agttactcct aataaataac
     2941 cataatatgt atttaccata acgcttcttg gccgcacccc ctgagagccc ctcagcgcgt
     3001 ttctgcggcc tgagggccgt ttccggcacc tttccccggc ccaaaccctg aaaaacgccc
     3061 ctgataattt ctgcgcggct gcatggctat gcacaagagt gaatggcggc cggtttcgtg
     3121 aaggaaatac cgtgaaagct ccgaaagaaa cgcttaattg cacagcagca catgaacgtg
     3181 atcagatgaa atctgtctac tgccgcactt cttcacgaaa ggcccgctga gcgtcagatt
     3241 cgggcctttc ttactaaaaa gatttcaata gaatttttta gcaggcgatt tttatctata
     3301 accatacatt gagcatagaa gacccaaatt tatgtatgac tcaatagaaa aaaacaaatc
     3361 acagccatct aaacactgag acagcaagcc aaacaaaaat caaagaaata actccatttt
     3421 taatcaaata atatagtttt cgattccttt tttttagctt ctgacctgca tcacgaatag
     3481 cattaacata tttaaataaa tgatttatag caccagtcct gtcattatta tcgttgggtg
     3541 atgctgccgc tgacattagc accccaccta tccacctatt tattttttgt gaccaccgcc
     3601 acttcatcgg cctttctata tcacagaaca aaataatacg tgtctgctcg gtatcattct
     3661 gagcccagtg aacaaaagtt tcgtcaaaaa ttatcacttc accatcatgc caactgcggc
     3721 gtacaccatc aacttcaata aaacaccgtt catcatttgg tgttgataat cctaaatgat
     3781 aacgaactga accggcataa gggtcacgat gctttcctaa atggcttcct gaaggcaatt
     3841 cagcaaacat cgctgcttta atacagggaa tagagctaac cagttgggtt gttactgggc
     3901 aaagccgctc cgctgatgga tgagcatctc cataccactt gaggtaaaag cgcttccaac
     3961 cgcgcttaaa aaatgtatta aatcctgcat cattattttt ttcagatgat ttaatgtgcc
     4021 cttgcaaatt aacagcttcg tctcttataa cctgccagtt atcaattaat ggttgaagtt
     4081 caggaaatgt ttctggggga aaccatgcct gctttgacgg cagcttcgaa aagcctgtca
     4141 taaacatatt tatcggagcc ataaatgttg aatgatcaaa taactgcctg gaaaactttt
     4201 gttttatttt accgcgagaa tgcgcataca atacactgat aagaaaaaca aaaataataa
     4261 taagtgacag catgtgtata ttccttgttc acgccaataa taaaaatctc ttaaagataa
     4321 cataatgaat gcacttaaat tataatctaa atgctattta tcaatagtat aatcgaagct
     4381 tcaccagaac atatctacta cacaggcaga gaggatttca gcaggaatta tgctgaaatg
     4441 aatcaaaggg acggaccgtg atatacccgc tgtcgctgtg atcgttcgag ctccagcgct
     4501 ttctgatgcc gcgcctcccg cacctgctgc agctcgacgg cattcagccg ctcgacaacc
     4561 ggcgcagctg atcgctcaaa ctctgcacct gcctgctcaa gcccgtgacg cgctcgctca
     4621 gcgccgcgtt ctcccgttgc ataagaccag acatcgtccg ccattccgcg aaggcgctct
     4681 cccactcgtc cagccttttc gagtagtcct gctgtagctg ctctaatgcg ctcagcaact
     4741 gtttttccag ctctgtcatg
//
