LOCUS       NZ_AP025902             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80334 plasmid pFJMB80334_3,
            complete sequence.
ACCESSION   NZ_AP025902
VERSION     NZ_AP025902.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490885
            Assembly: GCF_023735855.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 145x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735855.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:13:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,318
            CDSs (total)                      :: 5,203
            Genes (coding)                    :: 5,108
            CDSs (with protein)               :: 5,108
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 26 of 95
            Pseudo Genes (incomplete)         :: 73 of 95
            Pseudo Genes (internal stop)      :: 12 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025902.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80334"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80334_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-11-14"
     gene            800..943
                     /locus_tag="NGJ98_RS26540"
                     /old_locus_tag="FJMB80334_52230"
     CDS             800..943
                     /locus_tag="NGJ98_RS26540"
                     /old_locus_tag="FJMB80334_52230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1371..1598
                     /locus_tag="NGJ98_RS26545"
                     /old_locus_tag="FJMB80334_52240"
     CDS             1371..1598
                     /locus_tag="NGJ98_RS26545"
                     /old_locus_tag="FJMB80334_52240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1965..2150
                     /locus_tag="NGJ98_RS26550"
                     /old_locus_tag="FJMB80334_52250"
     CDS             1965..2150
                     /locus_tag="NGJ98_RS26550"
                     /old_locus_tag="FJMB80334_52250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2198..2277)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          405..965
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gtttcagaca aaatctgccg gtatgcatcc agcatcatgg ctccggcatc cccccggcac
       61 gccggagcca gcccgaaaca acagacacaa acaggtgtcg ctcatcatca agagtcatca
      121 ggtctccgga agaaaaacca aacatggctt acctcctgtt aacggcaacg cagaagaaag
      181 gtttacaggg tagcgggcag gaaaataaaa aaattgcagt cagcccggaa cggctgctga
      241 accggtacag gcgggttccg ggcgcagcgc cggaccagtc actcagcgct agcggagtgt
      301 atactggcta aaatgtaaca ccgcagtaac gcatagcata actgctacat aagacaggcg
      361 cagaaaatga aagaaaacgc gtcaggagaa aatgtaatac aggatatatt ccgcttcctc
      421 gctcactcac tcgctgcgct cggtcgttcg gctgcggcga gcggtacggg cttacgggtg
      481 gagcagaaat ttcctagggg atgccagcta ggggatgcca ggaagatact tagcgaagaa
      541 ggcataaggc cgcgccgaag ccgtttttcc ataggctccg cccccctgac gagcatcacg
      601 aaatctgacg ctcaaatcag tggtggcgaa acccgatgcg gcttaaagat accaggcgtt
      661 tccccctgat ggctccctcg tgctcccccg cattcctgcc tttcggttta ccggtgtcac
      721 tccgctgtta tggccgcggt ttctcattcc acgcctgaca ctcggttccg ggaaggcagt
      781 tcgctccagg atggactgta tgcacgaacc ccccgttcag tccgactgct gcgccttatc
      841 cggtaactat cgtcttgagt ccaacccgga aagacacgta aaaacgccac tggcagcggc
      901 cactggtaag gggtatacaa gattactggt gcagagttct tgaagttgac gcctgactgc
      961 ggctacacta gaaggacaaa tttggtggca gcggtcttgt acaccctgtt accacggttc
     1021 agaaacttgc cggtttgctg aaccttcgaa aaaccacctt gccgggtggt tttttcgttt
     1081 acagggcaag agattacgcg cgaaaaaaaa ggatctcaag aagatccttt tataaattag
     1141 acatccaacc tcacaaagag gaatacttaa atcatcttgg tatatgtatc ggtcggatta
     1201 catcagatat aagcccgcac tgccccctcc ccgccagaca ccacacttta accataacac
     1261 ctcgcaccta actcgatatg cagcccacca tcaagcctct tttcacataa aaaatgacct
     1321 atagttagat ggttagtgac caaaactgat atggataaaa ggagctggct ttgaaaaaga
     1381 tttttgtttc cgtgttcgca gcggcagttg cgctttcagc cctcaccggc tgcacgcgca
     1441 caagctatgc catacacacc aatgacgggc gcactattgt cagcgacggc aaacctaaag
     1501 aatcagactc aggcctgctt ggctatacag acgcgaatgg tgtcaaacag cagatcaata
     1561 aaaccgacgt taaagaagtc tccgaaattc cgcactgagc atcttctccg aaaaaggccg
     1621 ctaatgtggc ctttttcttt acctggggtt ctccgccaaa atcccagcaa attgcaccac
     1681 caaagctaaa agcttccagg gttgtctaaa aagcacaaga cattagagga agtgcggttt
     1741 ttataatcaa gccccccaag aatttaccat aacatccgtt gcccgcaccg cctcagacgc
     1801 actcagcgct tttctgacgg aaccatgacc aaaaaccaca acaaaccacc ccggaacccg
     1861 ccagaaacgc tcctgttaaa ttttaacggc atgcatgact atgcaccaga atgacggcat
     1921 gctcttttca cgcaaaaatc atcaccagac ggggaaaatc accagtgacc agacaggaat
     1981 ccgccgccct caacatggcc aaatttatcc gctcacagac actcctcctc ctcgagcggc
     2041 tcgaacagat ggatctggat gaggctgccg gctgctgtga gcacctgcac gatcaggccg
     2101 aagcacttta caccatgctg aacgcacaga caggcgagga agatgcgtga aaatcggtga
     2161 acgggtgcgc aattcagtgc gcggccgtga ggcgatggcg gggtgtcggg gcgcagccct
     2221 gacctgggta tttgtgatgc cggcgcgtgc gcggtattac aaatacacat cctgtcctga
     2281 gtggttcccc gggaacaggc aaaaaaaaac cggggagctg cccggaaggt aagtcaagtt
     2341 agattagatt actttcactc atccataaca gcatcatgca acgacgacca tcgtccgtga
     2401 cggccgcctc gtttaagtat ggacagaaat acagaaaatg ctcaggacga aatgtaatga
     2461 atgcgaacgg attcaagaaa ttcgagcatg accgtcctta cggccggttc a
//
