LOCUS       NZ_AP025898             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80191 plasmid pFJMB80191_3,
            complete sequence.
ACCESSION   NZ_AP025898
VERSION     NZ_AP025898.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490884
            Assembly: GCF_023735835.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 171x
            Sequencing Technology             :: NextSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735835.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:20:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,267
            CDSs (total)                      :: 5,152
            Genes (coding)                    :: 5,052
            CDSs (with protein)               :: 5,052
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 26 of 100
            Pseudo Genes (incomplete)         :: 77 of 100
            Pseudo Genes (internal stop)      :: 12 of 100
            Pseudo Genes (multiple problems)  :: 13 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025898.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80191"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80191_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-06-08"
     gene            801..944
                     /locus_tag="NGK38_RS26275"
                     /old_locus_tag="FJMB80191_51710"
     CDS             801..944
                     /locus_tag="NGK38_RS26275"
                     /old_locus_tag="FJMB80191_51710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1372..1599
                     /locus_tag="NGK38_RS26280"
                     /old_locus_tag="FJMB80191_51720"
     CDS             1372..1599
                     /locus_tag="NGK38_RS26280"
                     /old_locus_tag="FJMB80191_51720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1966..2151
                     /locus_tag="NGK38_RS26285"
                     /old_locus_tag="FJMB80191_51730"
     CDS             1966..2151
                     /locus_tag="NGK38_RS26285"
                     /old_locus_tag="FJMB80191_51730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2199..2278)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          406..966
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 agtttcagac aaaatctgcc ggtatgcatc cagcatcatg gctccggcat ccccccggca
       61 cgccggagcc agcccgaaac aacagacaca aacaggtgtc gctcatcatc aagagtcatc
      121 aggtctccgg aagaaaaacc aaacatggct tacctcctgt taacggcaac gcagaagaaa
      181 ggtttacagg gtagcgggca ggaaaataaa aaaattgcag tcagcccgga acggctgctg
      241 aaccggtaca ggcgggttcc gggcgcagcg ccggaccagt cactcagcgc tagcggagtg
      301 tatactggct aaaatgtaac accgcagtaa cgcatagcat aactgctaca taagacaggc
      361 gcagaaaatg aaagaaaacg cgtcaggaga aaatgtaata caggatatat tccgcttcct
      421 cgctcactca ctcgctgcgc tcggtcgttc ggctgcggcg agcggtacgg gcttacgggt
      481 ggagcagaaa tttcctaggg gatgccagct aggggatgcc aggaagatac ttagcgaaga
      541 aggcataagg ccgcgccgaa gccgtttttc cataggctcc gcccccctga cgagcatcac
      601 gaaatctgac gctcaaatca gtggtggcga aacccgatgc ggcttaaaga taccaggcgt
      661 ttccccctga tggctccctc gtgctccccc gcattcctgc ctttcggttt accggtgtca
      721 ctccgctgtt atggccgcgg tttctcattc cacgcctgac actcggttcc gggaaggcag
      781 ttcgctccag gatggactgt atgcacgaac cccccgttca gtccgactgc tgcgccttat
      841 ccggtaacta tcgtcttgag tccaacccgg aaagacacgt aaaaacgcca ctggcagcgg
      901 ccactggtaa ggggtataca agattactgg tgcagagttc ttgaagttga cgcctgactg
      961 cggctacact agaaggacaa atttggtggc agcggtcttg tacaccctgt taccacggtt
     1021 cagaaacttg ccggtttgct gaaccttcga aaaaccacct tgccgggtgg ttttttcgtt
     1081 tacagggcaa gagattacgc gcgaaaaaaa aggatctcaa gaagatcctt ttataaatta
     1141 gacatccaac ctcacaaaga ggaatactta aatcatcttg gtatatgtat cggtcggatt
     1201 acatcagata taagcccgca ctgccccctc cccgccagac accacacttt aaccataaca
     1261 cctcgcacct aactcgatat gcagcccacc atcaagcctc ttttcacata aaaaatgacc
     1321 tatagttaga tggttagtga ccaaaactga tatggataaa aggagctggc tttgaaaaag
     1381 atttttgttt ccgtgttcgc agcggcagtt gcgctttcag ccctcaccgg ctgcacgcgc
     1441 acaagctatg ccatacacac caatgacggg cgcactattg tcagcgacgg caaacctaaa
     1501 gaatcagact caggcctgct tggctataca gacgcgaatg gtgtcaaaca gcagatcaat
     1561 aaaaccgacg ttaaagaagt ctccgaaatt ccgcactgag catcttctcc gaaaaaggcc
     1621 gctaatgtgg cctttttctt tacctggggt tctccgccaa aatcccagca aattgcacca
     1681 ccaaagctaa aagcttccag ggttgtctaa aaagcacaag acattagagg aagtgcggtt
     1741 tttataatca agccccccaa gaatttacca taacatccgt tgcccgcacc gcctcagacg
     1801 cactcagcgc ttttctgacg gaaccatgac caaaaaccac aacaaaccac cccggaaccc
     1861 gccagaaacg ctcctgttaa attttaacgg catgcatgac tatgcaccag aatgacggca
     1921 tgctcttttc acgcaaaaat catcaccaga cggggaaaat caccagtgac cagacaggaa
     1981 tccgccgccc tcaacatggc caaatttatc cgctcacaga cactcctcct cctcgagcgg
     2041 ctcgaacaga tggatctgga tgaggctgcc ggctgctgtg agcacctgca cgatcaggcc
     2101 gaagcacttt acaccatgct gaacgcacag acaggcgagg aagatgcgtg aaaatcggtg
     2161 aacgggtgcg caattcagtg cgcggccgtg aggcgatggc ggggtgtcgg ggcgcagccc
     2221 tgacctgggt atttgtgatg ccggcgcgtg cgcggtatta caaatacaca tcctgtcctg
     2281 agtggttccc cgggaacagg caaaaaaaaa ccggggagct gcccggaagg taagtcaagt
     2341 tagattagat tactttcact catccataac agcatcatgc aacgacgacc atcgtccgtg
     2401 acggccgcct cgtttaagta tggacagaaa tacagaaaat gctcaggacg aaatgtaatg
     2461 aatgcgaacg gattcaagaa attcgagcat gaccgtcctt acggccggtt c
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