LOCUS       NZ_AP025897             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80191 plasmid pFJMB80191_2,
            complete sequence.
ACCESSION   NZ_AP025897
VERSION     NZ_AP025897.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490884
            Assembly: GCF_023735835.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 171x
            Sequencing Technology             :: NextSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735835.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:20:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,267
            CDSs (total)                      :: 5,152
            Genes (coding)                    :: 5,052
            CDSs (with protein)               :: 5,052
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 26 of 100
            Pseudo Genes (incomplete)         :: 77 of 100
            Pseudo Genes (internal stop)      :: 12 of 100
            Pseudo Genes (multiple problems)  :: 13 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025897.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80191"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80191_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-06-08"
     gene            complement(209..319)
                     /locus_tag="NGK38_RS26240"
     CDS             complement(209..319)
                     /locus_tag="NGK38_RS26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            963..1286
                     /locus_tag="NGK38_RS26245"
                     /old_locus_tag="FJMB80191_51670"
     CDS             963..1286
                     /locus_tag="NGK38_RS26245"
                     /old_locus_tag="FJMB80191_51670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1495..>1929
                     /locus_tag="NGK38_RS26250"
                     /pseudo=""
     CDS             1495..>1929
                     /locus_tag="NGK38_RS26250"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1964..2449
                     /locus_tag="NGK38_RS26255"
                     /old_locus_tag="FJMB80191_51680"
     CDS             1964..2449
                     /locus_tag="NGK38_RS26255"
                     /old_locus_tag="FJMB80191_51680"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2453..2668
                     /locus_tag="NGK38_RS26260"
                     /old_locus_tag="FJMB80191_51690"
     CDS             2453..2668
                     /locus_tag="NGK38_RS26260"
                     /old_locus_tag="FJMB80191_51690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2939..3853
                     /gene="lpxO"
                     /locus_tag="NGK38_RS26265"
     CDS             2939..3853
                     /gene="lpxO"
                     /locus_tag="NGK38_RS26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            4253..4714
                     /locus_tag="NGK38_RS26270"
     CDS             4253..4714
                     /locus_tag="NGK38_RS26270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     Typing          676..859
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(210..693)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          1277..2766
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagatcgttt tgctctgcgc gtaatctctt gctctgaaaa cgaaaaaacc acctggggag
       61 gtggtttttc gaaggttcag taagttgggg aacttctgaa ccgtggtaac agtcttgtgc
      121 gagacgtgtc accaaatctg tccttccagt gtagccgtag tccggccacc acttcaagaa
      181 ctctcgatac atctctcgca catcctgctt accagtggct accgccaatg gcgattttgt
      241 cgtgtctttc cgggttggac tcaagttgat agttaccgga atgggcgtgg tagtcggact
      301 gaacgggggg gtcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
      361 tcaggcgtgg aatgagacaa acgcggccac ataacagcgg aatgacaccg gtaaaccgaa
      421 aggcaggaac aggagagcgc acgagggagc taccaggggg aaacgcctgg gatctttata
      481 gtcctgtcgg gtttcgccgc cactgatttg agcgtcatat ttcgtgatgc ttgtcagggg
      541 ggcggagcct atggaaaaac ggcaacgccg catccttttc ccagcccttc ctgatttgag
      601 cgccatctct ttatcccgcc attcgtgagc cggtaacgcc cgccgcagtc tcacggcagg
      661 agcgtagcga ccgagtgagc gaggaggcgt catttcatcg tccccagcat ttcgcactta
      721 cgccccacag acgcattttt tacctgccac atgaagcact tccctgaatt cccacttcga
      781 tttaccatat taagccagta tacactccgc tagcgctacg tgactggttc agggctgcgc
      841 cccgaaaccc gctaaaacca ctgacgcgcc tgcggcttgt ccaacaccgc gccgaccggg
      901 aaaagatttc ccgcccgtcc cggcgttatc aggaggccgg attggcagac aaacgcagca
      961 aaatgctcac gatgtgggtg accgaggatg agcaccggcg tctgctggag cgctgcgacg
     1021 gtaaacagct tgcagcctgg atgcgccaga cgtgcctgga cgagaaaccc gcccgcgccg
     1081 gcaagcttcc ctcgatctcg ccggcgctgc ttcgtcagct tgccggcatg gggaacaacc
     1141 ttaaccagat tgcccggcag gttaacgccg gtggtggcag cgggcacgac cgcgtgcagg
     1201 tcgtcgccgc gctgatggcc atagatgccg gactcgagcg gctgcggcat gccgtgctgg
     1261 aaaagggtgc agacgatgat cgttaagttt cacccgcgag ggcgcggcgg tggcgccggt
     1321 ccggtggatt atctgctggg gaaagaccga cagcgcgagg gcgccaccgt tctgcagggg
     1381 aagccggaag aagtcaggga gcttatcgac gcctcaccct acgcaaaaaa gtacacatcc
     1441 ggcgtcctgt cctttgccga aaaggattta ccgcccgggc agcgcgaaaa gctgatggcg
     1501 agctttgaac gggttctgat gcccggactc gacaaagacc agtacagcgt gctgtgggtt
     1561 gagcaccagg ataaggggcg gctggagctg aacttcctga tcccgaacac ggagctgctg
     1621 actggcaagc gtctccagcc gtattacgac cgcgccgacc gtccgcgcat tgatgcctgg
     1681 cagaccatcg tgaacggcag gctggggctg catgacccga acgcgcctga aaaccggcgg
     1741 gcgctggtca cgccgtccgc gctgccggaa acgaagcagg aagccgctca ggcaattaca
     1801 cggggtttac tggccttggc ctcgtccggg gagctgaaaa cccgccagga cgtcactgag
     1861 gcgctggaaa gcgcaggttt tgaggtggta cgcaccacaa aaagcagcat cagcattgcc
     1921 gacccggacg gggggcgaaa catccgactg aagggagcca tctatgaaca gtcttttaac
     1981 gctggcgaag gacttagagc agaaatcgaa agcgcagcag cagagtaccg gcgagatgct
     2041 gaaagccgca ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg aaagcgagaa
     2101 gagaatcagc gccgccatcc tcgaccacga ccggaagctg tcctcagcca tgagccagcg
     2161 tacgaaaggg atgctgcgca tggtcagcca gacgtggctg accatcgtcc tggtctccgt
     2221 gctgctgatc gcctcgaacg cggccattct gtggtggcag agtcagcaga tactcgacaa
     2281 ttacgtgagc atccgggagc agaagagcac gcaggcgatg ctgtccgagc ggaacagcgg
     2341 cgtgcagctc tcgacctgcg gcgagcagag acgccgctgc gtgagggtga acccggaagc
     2401 gggacggttc ggagaggatt cgagctggat gatactggcg gggaaatagc acatgacaga
     2461 gctggaaaaa cagttgctga gcgcattaga gcagctacag caggactact cgaaaaggct
     2521 ggacgagtgg gagagcgcct tcgcggaatg gcggacgatg tctggtctta tgcaacggga
     2581 gaacgcggcg ctgagcgagc gcgtcacggg cttgagcagg caggtgcaga gtttgagcga
     2641 tcagctgcgc cggttgtcga gcggctgaat gccgtcgagc tgcagcaggt gcgggaggcg
     2701 cggcatcaga aagcgctgga gctcgaacga tcacagcgac agcgggtata tcacggtccg
     2761 tccctttgat tcatttcagc ataattcctg ctgaaatcct ctctgcctgt gtagtagata
     2821 tgttctggtg aagcttcgat tatactattg ataaatagca tttagattat aatttaagtg
     2881 cattcattat gttatcttta agagattttt attattggcg tgaacaagga atatacacat
     2941 gctgtcactt attattattt ttgtttttct tatcagtgta ttgtatgcgc attctcgcgg
     3001 taaaataaaa caaaagtttt ccaggcagtt atttgatcat tcaacattta tggctccgat
     3061 aaatatgttt atgacaggct tttcgaagct gccgtcaaag caggcatggt ttcccccaga
     3121 aacatttcct gaacttcaac cattaattga taactggcag gttataagag acgaagctgt
     3181 taatttgcaa gggcacatta aatcatctga aaaaaataat gatgcaggat ttaatacatt
     3241 ttttaagcgc ggttggaagc gcttttacct caagtggtat ggagatgctc atccatcagc
     3301 ggagcggctt tgcccagtaa caacccaact ggttagctct attccctgta ttaaagcagc
     3361 gatgtttgct gaattgcctt caggaagcca tttaggaaag catcgtgacc cttatgccgg
     3421 ttcagttcgt tatcatttag gattatcaac accaaatgat gaacggtgtt ttattgaagt
     3481 tgatggtgta cgccgcagtt ggcatgatgg tgaagtgata atttttgacg aaacttttgt
     3541 tcactgggct cagaatgata ccgagcagac acgtattatt ttgttctgtg atatagaaag
     3601 gccgatgaag tggcggtggt cacaaaaaat aaataggtgg ataggtgggg tgctaatgtc
     3661 agcggcagca tcacccaacg ataataatga caggactggt gctataaatc atttatttaa
     3721 atatgttaat gctattcgtg atgcaggtca gaagctaaaa aaaaggaatc gaaaactata
     3781 ttatttgatt aaaaatggag ttatttcttt gatttttgtt tggcttgctg tctcagtgtt
     3841 tagatggctg tgatttgttt ttttctattg agtcatacat aaatttgggt cttctatgct
     3901 caatgtatgg ttatagataa aaatcgcctg ctaaaaaatt ctattgaaat ctttttagta
     3961 agaaaggccc gaatctgacg ctcagcgggc ctttcgtgaa gaagtgcggc agtagacaga
     4021 tttcatctga tcacgttcat gtgctgctgt gcaattaagc gtttctttcg gagctttcac
     4081 ggtatttcct tcacgaaacc ggccgccatt cactcttgtg catagccatg cagccgcgca
     4141 gaaattatca ggggcgtttt tcagggtttg ggccggggaa aggtgccgga aacggccctc
     4201 aggccgcaga aacgcgctga ggggctctca gggggtgcgg ccaagaagcg ttatggtaaa
     4261 tacatattat ggttatttat taggagtaac tctgggagag tgggctatgg gatcagatgt
     4321 tcaatggcag atagataatg gttatttggc gcaagttatg ggggattttt cgacagagtc
     4381 gcctagcttt gaaacgccaa tgtttgggcg atattttgat acctttcaag ctgctataca
     4441 tgttgcaaaa gctttcccag ggagtacgtt gcagccatca gagtacgaag ataaaactgg
     4501 gatgctaata cgtcattatt atgtggattg tcatccgtat tatgatcgac tccctcctga
     4561 aaaaagcgat aattttcagt atgatgagta cccttctgct attcatgtgg ctgcgtgtat
     4621 tgatcaagct agcaaagaac tcgacgaact attgtttggt ggtggatatg gggaaaatgg
     4681 tgaggggcat gcaagcccga taattttata ttagtgttag gggcggaggg atattatcca
     4741 atttgtaaaa tgatcttctt
//
