LOCUS       NZ_AP025894             2511 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80182 plasmid pFJMB80182_3,
            complete sequence.
ACCESSION   NZ_AP025894
VERSION     NZ_AP025894.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490883
            Assembly: GCF_023735815.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 160x
            Sequencing Technology             :: NextSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735815.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:00:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,288
            CDSs (total)                      :: 5,173
            Genes (coding)                    :: 5,065
            CDSs (with protein)               :: 5,065
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 26 of 108
            Pseudo Genes (incomplete)         :: 85 of 108
            Pseudo Genes (internal stop)      :: 12 of 108
            Pseudo Genes (multiple problems)  :: 13 of 108
            Pseudo Genes (short protein)      :: 1 of 108
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025894.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80182"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80182_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-11-28"
     gene            complement(802..987)
                     /locus_tag="NGJ49_RS26390"
                     /old_locus_tag="FJMB80182_52020"
     CDS             complement(802..987)
                     /locus_tag="NGJ49_RS26390"
                     /old_locus_tag="FJMB80182_52020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1354..1581)
                     /locus_tag="NGJ49_RS26395"
                     /old_locus_tag="FJMB80182_52030"
     CDS             complement(1354..1581)
                     /locus_tag="NGJ49_RS26395"
                     /old_locus_tag="FJMB80182_52030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(2009..2152)
                     /locus_tag="NGJ49_RS26400"
                     /old_locus_tag="FJMB80182_52040"
     CDS             complement(2009..2152)
                     /locus_tag="NGJ49_RS26400"
                     /old_locus_tag="FJMB80182_52040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     Typing          676..755
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(1988..2511)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.713"
                     /coverage="85.0"
                     /evalue="1.5000000000000002e-168"
ORIGIN
        1 agcgcagcga gtgagtgagc gaggaagcgg aatatatcct gtattacatt ttctcctgac
       61 gcgttttctt tcattttctg cgcctgtctt atgtagcagt tatgctatgc gttactgcgg
      121 tgttacattt tagccagtat acactccgct agcgctgagt gactggtccg gcgctgcgcc
      181 cggaacccgc ctgtaccggt tcagcagccg ttccgggctg actgcaattt ttttattttc
      241 ctgcccgcta ccctgtaaac ctttcttctg cgttgccgtt aacaggaggt aagccatgtt
      301 tggtttttct tccggagacc tgatgactct tgatgatgag cgacacctgt ttgtgtctgt
      361 tgtttcgggc tggctccggc gtgccggggg gatgccggag ccatgatgct ggatgcatac
      421 cggcagattt tgtctgaaac tgaaccggcc gtaaggacgg tcatgctcga atttcttgaa
      481 tccgttcgca ttcattacat ttcgtcctga gcattttctg tatttctgtc catacttaaa
      541 cgaggcggcc gtcacggacg atggtcgtcg ttgcatgatg ctgttatgga tgagtgaaag
      601 taatctaatc taacttgact taccttccgg gcagctcccc ggtttttttt tgcctgttcc
      661 cggggaacca ctcaggacag gatgtgtatt tgtaataccg cgcacgcgcc ggcatcacaa
      721 atacccaggt cagggctgcg ccccgacacc ccgccatcgc ctcacggccg cgcactgaat
      781 tgcgcacccg ttcaccgatt ttcacgcatc ttcctcgcct gtctgtgcgt tcagcatggt
      841 gtaaagtgct tcggcctgat cgtgcaggtg ctcacagcag ccggcagcct catccagatc
      901 catctgttcg agccgctcga ggaggaggag tgtctgtgag cggataaatt tggccatgtt
      961 gagggcggcg gattcctgtc tggtcactgg tgattttccc cgtctggtga tgatttttgc
     1021 gtgaaaagag catgccgtca ttctggtgca tagtcatgca tgccgttaaa atttaacagg
     1081 agcgtttctg gcgggttccg gggtggtttg ttgtggtttt tggtcatggt tccgtcagaa
     1141 aagcgctgag tgcgtctgag gcggtgcggg caacggatgt tatggtaaat tcttgggggg
     1201 cttgattata aaaaccgcac ttcctctaat gtcttgtgct ttttagacaa ccctggaagc
     1261 ttttagcttt ggtggtgcaa tttgctggga ttttggcgga gaaccccagg taaagaaaaa
     1321 ggccacatta gcggcctttt tcggagaaga tgctcagtgc ggaatttcgg agacttcttt
     1381 aacgtcggtt ttattgatct gctgtttgac accattcgcg tctgtatagc caagcaggcc
     1441 tgagtctgat tctttaggtt tgccgtcgct gacaatagtg cgcccgtcat tggtgtgtat
     1501 ggcatagctt gtgcgcgtgc agccggtgag ggctgaaagc gcaactgccg ctgcgaacac
     1561 ggaaacaaaa atctttttca aagccagctc cttttatcca tatcagtttt ggtcactaac
     1621 catctaacta taggtcattt tttatgtgaa aagaggcttg atggtgggct gcatatcgag
     1681 ttaggtgcga ggtgttatgg ttaaagtgtg gtgtctggcg gggagggggc agtgcgggct
     1741 tatatctgat gtaatccgac cgatacatat accaagatga tttaagtatt cctctttgtg
     1801 aggttggatg tctaatttat aaaaggatct tcttgagatc cttttttttc gcgcgtaatc
     1861 tcttgccctg taaacgaaaa aaccacccgg caaggtggtt tttcgaaggt tcagcaaacc
     1921 ggcaagtttc tgaaccgtgg taacagggtg tacaagaccg ctgccaccaa atttgtcctt
     1981 ctagtgtagc cgcagtcagg cgtcaacttc aagaactctg caccagtaat cttgtatacc
     2041 ccttaccagt ggccgctgcc agtggcgttt ttacgtgtct ttccgggttg gactcaagac
     2101 gatagttacc ggataaggcg cagcagtcgg actgaacggg gggttcgtgc atacagtcca
     2161 tcctggagcg aactgccttc ccggaaccga gtgtcaggcg tggaatgaga aaccgcggcc
     2221 ataacagcgg agtgacaccg gtaaaccgaa aggcaggaat gcgggggagc acgagggagc
     2281 catcaggggg aaacgcctgg tatctttaag ccgcatcggg tttcgccacc actgatttga
     2341 gcgtcagatt tcgtgatgct cgtcaggggg gcggagccta tggaaaaacg gcttcggcgc
     2401 ggccttatgc cttcttcgct aagtatcttc ctggcatccc ctagctggca tcccctagga
     2461 aatttctgct ccacccgtaa gcccgtaccg ctcgccgcag ccgaacgacc g
//
