LOCUS       NZ_AP025893             4760 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80182 plasmid pFJMB80182_2,
            complete sequence.
ACCESSION   NZ_AP025893
VERSION     NZ_AP025893.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490883
            Assembly: GCF_023735815.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 160x
            Sequencing Technology             :: NextSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735815.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:00:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,288
            CDSs (total)                      :: 5,173
            Genes (coding)                    :: 5,065
            CDSs (with protein)               :: 5,065
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 26 of 108
            Pseudo Genes (incomplete)         :: 85 of 108
            Pseudo Genes (internal stop)      :: 12 of 108
            Pseudo Genes (multiple problems)  :: 13 of 108
            Pseudo Genes (short protein)      :: 1 of 108
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025893.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80182"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80182_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-11-28"
     gene            complement(73..318)
                     /locus_tag="NGJ49_RS26355"
     CDS             complement(73..318)
                     /locus_tag="NGJ49_RS26355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023343077.1"
                     /translation="MQPQPAVHDGLPGINARTVGAVVIRLETLASQQLRVRDQEVQLQP
                     PLILVLNPQHAVLVFVESGHQNPFKARHQLFALPGR"
                     /name="hypothetical protein"
     gene            569..1054
                     /locus_tag="NGJ49_RS26360"
                     /old_locus_tag="FJMB80182_51970"
     CDS             569..1054
                     /locus_tag="NGJ49_RS26360"
                     /old_locus_tag="FJMB80182_51970"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1058..1273
                     /locus_tag="NGJ49_RS26365"
                     /old_locus_tag="FJMB80182_51980"
     CDS             1058..1273
                     /locus_tag="NGJ49_RS26365"
                     /old_locus_tag="FJMB80182_51980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1544..2458
                     /gene="lpxO"
                     /locus_tag="NGJ49_RS26370"
     CDS             1544..2458
                     /gene="lpxO"
                     /locus_tag="NGJ49_RS26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            2858..3319
                     /locus_tag="NGJ49_RS26375"
     CDS             2858..3319
                     /locus_tag="NGJ49_RS26375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(3574..3684)
                     /locus_tag="NGJ49_RS26380"
     CDS             complement(3574..3684)
                     /locus_tag="NGJ49_RS26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            4328..4651
                     /locus_tag="NGJ49_RS26385"
                     /old_locus_tag="FJMB80182_52000"
     CDS             4328..4651
                     /locus_tag="NGJ49_RS26385"
                     /old_locus_tag="FJMB80182_52000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4041..4224
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(3575..4058)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          2..1371
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.285"
                     /coverage="92.0"
                     /evalue="0.0"
     Typing          4329..4648
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="1.6200000000000003e-64"
ORIGIN
        1 agggagctta tcgacgcctc accctacgca aaaaagtaca catccggcgt cctgtccttt
       61 gccgaaaagg atttaccgcc cgggcagcgc gaaaagctga tggcgagctt tgaacgggtt
      121 ctgatgcccg gactcgacaa agaccagtac agcgtgctgt gggttgagca ccaggataag
      181 gggcggctgg agctgaactt cctgatcccg aacacggagc tgctgactgg caagcgtctc
      241 cagccgtatt acgaccgcgc cgaccgtccg cgcattgatg cctggcagac catcgtgaac
      301 ggcaggctgg ggctgcatga cccgaacgcg cctgaaaacc ggcgggcgct ggtcacgccg
      361 tccgcgctgc cggaaacgaa gcaggaagcc gctcaggcaa ttacacgggg tttactggcc
      421 ttggcctcgt ccggggagct gaaaacccgc caggacgtca ctgaggcgct ggaaagcgca
      481 ggttttgagg tggtacgcac cacaaaaagc agcatcagca ttgccgaccc ggacgggggg
      541 cgaaacatcc gactgaaggg agccatctat gaacagtctt ttaacgctgg cgaaggactt
      601 agagcagaaa tcgaaagcgc agcagcagag taccggcgag atgctgaaag ccgcattcag
      661 cgagcacgag aagtctgtca gagcggaact gagcgaaagc gagaagagaa tcagcgccgc
      721 catcctcgac cacgaccgga agctgtcctc agccatgagc cagcgtacga aagggatgct
      781 gcgcatggtc agccagacgt ggctgaccat cgtcctggtc tccgtgctgc tgatcgcctc
      841 gaacgcggcc attctgtggt ggcagagtca gcagatactc gacaattacg tgagcatccg
      901 ggagcagaag agcacgcagg cgatgctgtc cgagcggaac agcggcgtgc agctctcgac
      961 ctgcggcgag cagagacgcc gctgcgtgag ggtgaacccg gaagcgggac ggttcggaga
     1021 ggattcgagc tggatgatac tggcggggaa atagcacatg acagagctgg aaaaacagtt
     1081 gctgagcgca ttagagcagc tacagcagga ctactcgaaa aggctggacg agtgggagag
     1141 cgccttcgcg gaatggcgga cgatgtctgg tcttatgcaa cgggagaacg cggcgctgag
     1201 cgagcgcgtc acgggcttga gcaggcaggt gcagagtttg agcgatcagc tgcgccggtt
     1261 gtcgagcggc tgaatgccgt cgagctgcag caggtgcggg aggcgcggca tcagaaagcg
     1321 ctggagctcg aacgatcaca gcgacagcgg gtatatcacg gtccgtccct ttgattcatt
     1381 tcagcataat tcctgctgaa atcctctctg cctgtgtagt agatatgttc tggtgaagct
     1441 tcgattatac tattgataaa tagcatttag attataattt aagtgcattc attatgttat
     1501 ctttaagaga tttttattat tggcgtgaac aaggaatata cacatgctgt cacttattat
     1561 tatttttgtt tttcttatca gtgtattgta tgcgcattct cgcggtaaaa taaaacaaaa
     1621 gttttccagg cagttatttg atcattcaac atttatggct ccgataaata tgtttatgac
     1681 aggcttttcg aagctgccgt caaagcaggc atggtttccc ccagaaacat ttcctgaact
     1741 tcaaccatta attgataact ggcaggttat aagagacgaa gctgttaatt tgcaagggca
     1801 cattaaatca tctgaaaaaa ataatgatgc aggatttaat acatttttta agcgcggttg
     1861 gaagcgcttt tacctcaagt ggtatggaga tgctcatcca tcagcggagc ggctttgccc
     1921 agtaacaacc caactggtta gctctattcc ctgtattaaa gcagcgatgt ttgctgaatt
     1981 gccttcagga agccatttag gaaagcatcg tgacccttat gccggttcag ttcgttatca
     2041 tttaggatta tcaacaccaa atgatgaacg gtgttttatt gaagttgatg gtgtacgccg
     2101 cagttggcat gatggtgaag tgataatttt tgacgaaact tttgttcact gggctcagaa
     2161 tgataccgag cagacacgta ttattttgtt ctgtgatata gaaaggccga tgaagtggcg
     2221 gtggtcacaa aaaataaata ggtggatagg tggggtgcta atgtcagcgg cagcatcacc
     2281 caacgataat aatgacagga ctggtgctat aaatcattta tttaaatatg ttaatgctat
     2341 tcgtgatgca ggtcagaagc taaaaaaaag gaatcgaaaa ctatattatt tgattaaaaa
     2401 tggagttatt tctttgattt ttgtttggct tgctgtctca gtgtttagat ggctgtgatt
     2461 tgtttttttc tattgagtca tacataaatt tgggtcttct atgctcaatg tatggttata
     2521 gataaaaatc gcctgctaaa aaattctatt gaaatctttt tagtaagaaa ggcccgaatc
     2581 tgacgctcag cgggcctttc gtgaagaagt gcggcagtag acagatttca tctgatcacg
     2641 ttcatgtgct gctgtgcaat taagcgtttc tttcggagct ttcacggtat ttccttcacg
     2701 aaaccggccg ccattcactc ttgtgcatag ccatgcagcc gcgcagaaat tatcaggggc
     2761 gtttttcagg gtttgggccg gggaaaggtg ccggaaacgg ccctcaggcc gcagaaacgc
     2821 gctgaggggc tctcaggggg tgcggccaag aagcgttatg gtaaatacat attatggtta
     2881 tttattagga gtaactctgg gagagtgggc tatgggatca gatgttcaat ggcagataga
     2941 taatggttat ttggcgcaag ttatggggga tttttcgaca gagtcgccta gctttgaaac
     3001 gccaatgttt gggcgatatt ttgatacctt tcaagctgct atacatgttg caaaagcttt
     3061 cccagggagt acgttgcagc catcagagta cgaagataaa actgggatgc taatacgtca
     3121 ttattatgtg gattgtcatc cgtattatga tcgactccct cctgaaaaaa gcgataattt
     3181 tcagtatgat gagtaccctt ctgctattca tgtggctgcg tgtattgatc aagctagcaa
     3241 agaactcgac gaactattgt ttggtggtgg atatggggaa aatggtgagg ggcatgcaag
     3301 cccgataatt ttatattagt gttaggggcg gagggatatt atccaatttg taaaatgatc
     3361 ttcttgagat cgttttgctc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccacctg
     3421 gggaggtggt ttttcgaagg ttcagtaagt tggggaactt ctgaaccgtg gtaacagtct
     3481 tgtgcgagac gtgtcaccaa atctgtcctt ccagtgtagc cgtagtccgg ccaccacttc
     3541 aagaactctc gatacatctc tcgcacatcc tgcttaccag tggctaccgc caatggcgat
     3601 tttgtcgtgt ctttccgggt tggactcaag ttgatagtta ccggaatggg cgtggtagtc
     3661 ggactgaacg ggggggtcgt gcatacagtc cagcttggag cgaactgcct acccggaact
     3721 gagtgtcagg cgtggaatga gacaaacgcg gccacataac agcggaatga caccggtaaa
     3781 ccgaaaggca ggaacaggag agcgcacgag ggagctacca gggggaaacg cctgggatct
     3841 ttatagtcct gtcgggtttc gccgccactg atttgagcgt catatttcgt gatgcttgtc
     3901 aggggggcgg agcctatgga aaaacggcaa cgccgcatcc ttttcccagc ccttcctgat
     3961 ttgagcgcca tctctttatc ccgccattcg tgagccggta acgcccgccg cagtctcacg
     4021 gcaggagcgt agcgaccgag tgagcgagga ggcgtcattt catcgtcccc agcatttcgc
     4081 acttacgccc cacagacgca ttttttacct gccacatgaa gcacttccct gaattcccac
     4141 ttcgatttac catattaagc cagtatacac tccgctagcg ctacgtgact ggttcagggc
     4201 tgcgccccga aacccgctaa aaccactgac gcgcctgcgg cttgtccaac accgcgccga
     4261 ccgggaaaag atttcccgcc cgtcccggcg ttatcaggag gccggattgg cagacaaacg
     4321 cagcaaaatg ctcacgatgt gggtgaccga ggatgagcac cggcgtctgc tggagcgctg
     4381 cgacggtaaa cagcttgcag cctggatgcg ccagacgtgc ctggacgaga aacccgcccg
     4441 cgccggcaag cttccctcga tctcgccggc gctgcttcgt cagcttgccg gcatggggaa
     4501 caaccttaac cagattgccc ggcaggttaa cgccggtggt ggcagcgggc acgaccgcgt
     4561 gcaggtcgtc gccgcgctga tggccataga tgccggactc gagcggctgc ggcatgccgt
     4621 gctggaaaag ggtgcagacg atgatcgtta agtttcaccc gcgagggcgc ggcggtggcg
     4681 ccggtccggt ggattatctg ctggggaaag accgacagcg cgagggcgcc accgttctgc
     4741 aggggaagcc ggaagaagtc
//
