LOCUS       NZ_AP025890             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80178 plasmid pFJMB80178_3,
            complete sequence.
ACCESSION   NZ_AP025890
VERSION     NZ_AP025890.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490882
            Assembly: GCF_023735795.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 121x
            Sequencing Technology             :: NextSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735795.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:26:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,259
            CDSs (total)                      :: 5,144
            Genes (coding)                    :: 5,051
            CDSs (with protein)               :: 5,051
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 28 of 93
            Pseudo Genes (incomplete)         :: 68 of 93
            Pseudo Genes (internal stop)      :: 12 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            Pseudo Genes (short protein)      :: 1 of 93
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025890.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80178"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80178_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-11-01"
     gene            complement(join(2443..2511,1..75))
                     /locus_tag="NGK69_RS26235"
     CDS             complement(join(2443..2511,1..75))
                     /locus_tag="NGK69_RS26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(1236..1421)
                     /locus_tag="NGK69_RS26240"
                     /old_locus_tag="FJMB80178_51740"
     CDS             complement(1236..1421)
                     /locus_tag="NGK69_RS26240"
                     /old_locus_tag="FJMB80178_51740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1788..2015)
                     /locus_tag="NGK69_RS26245"
                     /old_locus_tag="FJMB80178_51750"
     CDS             complement(1788..2015)
                     /locus_tag="NGK69_RS26245"
                     /old_locus_tag="FJMB80178_51750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1110..1189
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(4..478)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.121"
                     /coverage="88.0"
                     /evalue="4.69e-163"
     Typing          complement(2261..2454)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.935"
                     /coverage="86.0"
                     /evalue="1.52e-53"
ORIGIN
        1 tctttccggg ttggactcaa gacgatagtt accggataag gcgcagcagt cggactgaac
       61 ggggggttcg tgcatacagt ccatcctgga gcgaactgcc ttcccggaac cgagtgtcag
      121 gcgtggaatg agaaaccgcg gccataacag cggagtgaca ccggtaaacc gaaaggcagg
      181 aatgcggggg agcacgaggg agccatcagg gggaaacgcc tggtatcttt aagccgcatc
      241 gggtttcgcc accactgatt tgagcgtcag atttcgtgat gctcgtcagg ggggcggagc
      301 ctatggaaaa acggcttcgg cgcggcctta tgccttcttc gctaagtatc ttcctggcat
      361 cccctagctg gcatccccta ggaaatttct gctccacccg taagcccgta ccgctcgccg
      421 cagccgaacg accgagcgca gcgagtgagt gagcgaggaa gcggaatata tcctgtatta
      481 cattttctcc tgacgcgttt tctttcattt tctgcgcctg tcttatgtag cagttatgct
      541 atgcgttact gcggtgttac attttagcca gtatacactc cgctagcgct gagtgactgg
      601 tccggcgctg cgcccggaac ccgcctgtac cggttcagca gccgttccgg gctgactgca
      661 atttttttat tttcctgccc gctaccctgt aaacctttct tctgcgttgc cgttaacagg
      721 aggtaagcca tgtttggttt ttcttccgga gacctgatga ctcttgatga tgagcgacac
      781 ctgtttgtgt ctgttgtttc gggctggctc cggcgtgccg gggggatgcc ggagccatga
      841 tgctggatgc ataccggcag attttgtctg aaactgaacc ggccgtaagg acggtcatgc
      901 tcgaatttct tgaatccgtt cgcattcatt acatttcgtc ctgagcattt tctgtatttc
      961 tgtccatact taaacgaggc ggccgtcacg gacgatggtc gtcgttgcat gatgctgtta
     1021 tggatgagtg aaagtaatct aatctaactt gacttacctt ccgggcagct ccccggtttt
     1081 tttttgcctg ttcccgggga accactcagg acaggatgtg tatttgtaat accgcgcacg
     1141 cgccggcatc acaaataccc aggtcagggc tgcgccccga caccccgcca tcgcctcacg
     1201 gccgcgcact gaattgcgca cccgttcacc gattttcacg catcttcctc gcctgtctgt
     1261 gcgttcagca tggtgtaaag tgcttcggcc tgatcgtgca ggtgctcaca gcagccggca
     1321 gcctcatcca gatccatctg ttcgagccgc tcgaggagga ggagtgtctg tgagcggata
     1381 aatttggcca tgttgagggc ggcggattcc tgtctggtca ctggtgattt tccccgtctg
     1441 gtgatgattt ttgcgtgaaa agagcatgcc gtcattctgg tgcatagtca tgcatgccgt
     1501 taaaatttaa caggagcgtt tctggcgggt tccggggtgg tttgttgtgg tttttggtca
     1561 tggttccgtc agaaaagcgc tgagtgcgtc tgaggcggtg cgggcaacgg atgttatggt
     1621 aaattcttgg ggggcttgat tataaaaacc gcacttcctc taatgtcttg tgctttttag
     1681 acaaccctgg aagcttttag ctttggtggt gcaatttgct gggattttgg cggagaaccc
     1741 caggtaaaga aaaaggccac attagcggcc tttttcggag aagatgctca gtgcggaatt
     1801 tcggagactt ctttaacgtc ggttttattg atctgctgtt tgacaccatt cgcgtctgta
     1861 tagccaagca ggcctgagtc tgattcttta ggtttgccgt cgctgacaat agtgcgcccg
     1921 tcattggtgt gtatggcata gcttgtgcgc gtgcagccgg tgagggctga aagcgcaact
     1981 gccgctgcga acacggaaac aaaaatcttt ttcaaagcca gctcctttta tccatatcag
     2041 ttttggtcac taaccatcta actataggtc attttttatg tgaaaagagg cttgatggtg
     2101 ggctgcatat cgagttaggt gcgaggtgtt atggttaaag tgtggtgtct ggcggggagg
     2161 gggcagtgcg ggcttatatc tgatgtaatc cgaccgatac atataccaag atgatttaag
     2221 tattcctctt tgtgaggttg gatgtctaat ttataaaagg atcttcttga gatccttttt
     2281 tttcgcgcgt aatctcttgc cctgtaaacg aaaaaaccac ccggcaaggt ggtttttcga
     2341 aggttcagca aaccggcaag tttctgaacc gtggtaacag ggtgtacaag accgctgcca
     2401 ccaaatttgt ccttctagtg tagccgcagt caggcgtcaa cttcaagaac tctgcaccag
     2461 taatcttgta taccccttac cagtggccgc tgccagtggc gtttttacgt g
//
