LOCUS       NZ_AP025886             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80158 plasmid pFJMB80158_3,
            complete sequence.
ACCESSION   NZ_AP025886
VERSION     NZ_AP025886.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490881
            Assembly: GCF_023735775.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 143x
            Sequencing Technology             :: NextSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735775.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:11:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,193
            CDSs (total)                      :: 5,078
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 30 of 93
            Pseudo Genes (incomplete)         :: 67 of 93
            Pseudo Genes (internal stop)      :: 12 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            Pseudo Genes (short protein)      :: 1 of 93
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025886.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80158"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80158_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-05-23"
     gene            791..934
                     /locus_tag="NGK56_RS25905"
     CDS             791..934
                     /locus_tag="NGK56_RS25905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1362..1589
                     /locus_tag="NGK56_RS25910"
                     /old_locus_tag="FJMB80158_50980"
     CDS             1362..1589
                     /locus_tag="NGK56_RS25910"
                     /old_locus_tag="FJMB80158_50980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1956..2141
                     /locus_tag="NGK56_RS25915"
                     /old_locus_tag="FJMB80158_50990"
     CDS             1956..2141
                     /locus_tag="NGK56_RS25915"
                     /old_locus_tag="FJMB80158_50990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2189..2268)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          396..956
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 aaaatctgcc ggtatgcatc cagcatcatg gctccggcat ccccccggca cgccggagcc
       61 agcccgaaac aacagacaca aacaggtgtc gctcatcatc aagagtcatc aggtctccgg
      121 aagaaaaacc aaacatggct tacctcctgt taacggcaac gcagaagaaa ggtttacagg
      181 gtagcgggca ggaaaataaa aaaattgcag tcagcccgga acggctgctg aaccggtaca
      241 ggcgggttcc gggcgcagcg ccggaccagt cactcagcgc tagcggagtg tatactggct
      301 aaaatgtaac accgcagtaa cgcatagcat aactgctaca taagacaggc gcagaaaatg
      361 aaagaaaacg cgtcaggaga aaatgtaata caggatatat tccgcttcct cgctcactca
      421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtacgg gcttacgggt ggagcagaaa
      481 tttcctaggg gatgccagct aggggatgcc aggaagatac ttagcgaaga aggcataagg
      541 ccgcgccgaa gccgtttttc cataggctcc gcccccctga cgagcatcac gaaatctgac
      601 gctcaaatca gtggtggcga aacccgatgc ggcttaaaga taccaggcgt ttccccctga
      661 tggctccctc gtgctccccc gcattcctgc ctttcggttt accggtgtca ctccgctgtt
      721 atggccgcgg tttctcattc cacgcctgac actcggttcc gggaaggcag ttcgctccag
      781 gatggactgt atgcacgaac cccccgttca gtccgactgc tgcgccttat ccggtaacta
      841 tcgtcttgag tccaacccgg aaagacacgt aaaaacgcca ctggcagcgg ccactggtaa
      901 ggggtataca agattactgg tgcagagttc ttgaagttga cgcctgactg cggctacact
      961 agaaggacaa atttggtggc agcggtcttg tacaccctgt taccacggtt cagaaacttg
     1021 ccggtttgct gaaccttcga aaaaccacct tgccgggtgg ttttttcgtt tacagggcaa
     1081 gagattacgc gcgaaaaaaa aggatctcaa gaagatcctt ttataaatta gacatccaac
     1141 ctcacaaaga ggaatactta aatcatcttg gtatatgtat cggtcggatt acatcagata
     1201 taagcccgca ctgccccctc cccgccagac accacacttt aaccataaca cctcgcacct
     1261 aactcgatat gcagcccacc atcaagcctc ttttcacata aaaaatgacc tatagttaga
     1321 tggttagtga ccaaaactga tatggataaa aggagctggc tttgaaaaag atttttgttt
     1381 ccgtgttcgc agcggcagtt gcgctttcag ccctcaccgg ctgcacgcgc acaagctatg
     1441 ccatacacac caatgacggg cgcactattg tcagcgacgg caaacctaaa gaatcagact
     1501 caggcctgct tggctataca gacgcgaatg gtgtcaaaca gcagatcaat aaaaccgacg
     1561 ttaaagaagt ctccgaaatt ccgcactgag catcttctcc gaaaaaggcc gctaatgtgg
     1621 cctttttctt tacctggggt tctccgccaa aatcccagca aattgcacca ccaaagctaa
     1681 aagcttccag ggttgtctaa aaagcacaag acattagagg aagtgcggtt tttataatca
     1741 agccccccaa gaatttacca taacatccgt tgcccgcacc gcctcagacg cactcagcgc
     1801 ttttctgacg gaaccatgac caaaaaccac aacaaaccac cccggaaccc gccagaaacg
     1861 ctcctgttaa attttaacgg catgcatgac tatgcaccag aatgacggca tgctcttttc
     1921 acgcaaaaat catcaccaga cggggaaaat caccagtgac cagacaggaa tccgccgccc
     1981 tcaacatggc caaatttatc cgctcacaga cactcctcct cctcgagcgg ctcgaacaga
     2041 tggatctgga tgaggctgcc ggctgctgtg agcacctgca cgatcaggcc gaagcacttt
     2101 acaccatgct gaacgcacag acaggcgagg aagatgcgtg aaaatcggtg aacgggtgcg
     2161 caattcagtg cgcggccgtg aggcgatggc ggggtgtcgg ggcgcagccc tgacctgggt
     2221 atttgtgatg ccggcgcgtg cgcggtatta caaatacaca tcctgtcctg agtggttccc
     2281 cgggaacagg caaaaaaaaa ccggggagct gcccggaagg taagtcaagt tagattagat
     2341 tactttcact catccataac agcatcatgc aacgacgacc atcgtccgtg acggccgcct
     2401 cgtttaagta tggacagaaa tacagaaaat gctcaggacg aaatgtaatg aatgcgaacg
     2461 gattcaagaa attcgagcat gaccgtcctt acggccggtt cagtttcaga c
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