LOCUS       NZ_AP025885             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80158 plasmid pFJMB80158_2,
            complete sequence.
ACCESSION   NZ_AP025885
VERSION     NZ_AP025885.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490881
            Assembly: GCF_023735775.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 143x
            Sequencing Technology             :: NextSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735775.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:11:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,193
            CDSs (total)                      :: 5,078
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 30 of 93
            Pseudo Genes (incomplete)         :: 67 of 93
            Pseudo Genes (internal stop)      :: 12 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            Pseudo Genes (short protein)      :: 1 of 93
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025885.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80158"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80158_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-05-23"
     gene            join(4503..4760,1..228)
                     /locus_tag="NGK56_RS25870"
     CDS             join(4503..4760,1..228)
                     /locus_tag="NGK56_RS25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            232..447
                     /locus_tag="NGK56_RS25875"
                     /old_locus_tag="FJMB80158_50940"
     CDS             232..447
                     /locus_tag="NGK56_RS25875"
                     /old_locus_tag="FJMB80158_50940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            718..1632
                     /gene="lpxO"
                     /locus_tag="NGK56_RS25880"
     CDS             718..1632
                     /gene="lpxO"
                     /locus_tag="NGK56_RS25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            2032..2493
                     /locus_tag="NGK56_RS25885"
     CDS             2032..2493
                     /locus_tag="NGK56_RS25885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(2748..2858)
                     /locus_tag="NGK56_RS25890"
     CDS             complement(2748..2858)
                     /locus_tag="NGK56_RS25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            3502..3825
                     /locus_tag="NGK56_RS25895"
                     /old_locus_tag="FJMB80158_50960"
     CDS             3502..3825
                     /locus_tag="NGK56_RS25895"
                     /old_locus_tag="FJMB80158_50960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4034..>4468
                     /locus_tag="NGK56_RS25900"
                     /pseudo=""
     CDS             4034..>4468
                     /locus_tag="NGK56_RS25900"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3215..3398
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(2749..3232)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          4..545
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.425"
                     /coverage="99.0"
                     /evalue="1.65e-83"
     Typing          3816..4756
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.134"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgctgatcg cctcgaacgc ggccattctg tggtggcaga gtcagcagat actcgacaat
       61 tacgtgagca tccgggagca gaagagcacg caggcgatgc tgtccgagcg gaacagcggc
      121 gtgcagctct cgacctgcgg cgagcagaga cgccgctgcg tgagggtgaa cccggaagcg
      181 ggacggttcg gagaggattc gagctggatg atactggcgg ggaaatagca catgacagag
      241 ctggaaaaac agttgctgag cgcattagag cagctacagc aggactactc gaaaaggctg
      301 gacgagtggg agagcgcctt cgcggaatgg cggacgatgt ctggtcttat gcaacgggag
      361 aacgcggcgc tgagcgagcg cgtcacgggc ttgagcaggc aggtgcagag tttgagcgat
      421 cagctgcgcc ggttgtcgag cggctgaatg ccgtcgagct gcagcaggtg cgggaggcgc
      481 ggcatcagaa agcgctggag ctcgaacgat cacagcgaca gcgggtatat cacggtccgt
      541 ccctttgatt catttcagca taattcctgc tgaaatcctc tctgcctgtg tagtagatat
      601 gttctggtga agcttcgatt atactattga taaatagcat ttagattata atttaagtgc
      661 attcattatg ttatctttaa gagattttta ttattggcgt gaacaaggaa tatacacatg
      721 ctgtcactta ttattatttt tgtttttctt atcagtgtat tgtatgcgca ttctcgcggt
      781 aaaataaaac aaaagttttc caggcagtta tttgatcatt caacatttat ggctccgata
      841 aatatgttta tgacaggctt ttcgaagctg ccgtcaaagc aggcatggtt tcccccagaa
      901 acatttcctg aacttcaacc attaattgat aactggcagg ttataagaga cgaagctgtt
      961 aatttgcaag ggcacattaa atcatctgaa aaaaataatg atgcaggatt taatacattt
     1021 tttaagcgcg gttggaagcg cttttacctc aagtggtatg gagatgctca tccatcagcg
     1081 gagcggcttt gcccagtaac aacccaactg gttagctcta ttccctgtat taaagcagcg
     1141 atgtttgctg aattgccttc aggaagccat ttaggaaagc atcgtgaccc ttatgccggt
     1201 tcagttcgtt atcatttagg attatcaaca ccaaatgatg aacggtgttt tattgaagtt
     1261 gatggtgtac gccgcagttg gcatgatggt gaagtgataa tttttgacga aacttttgtt
     1321 cactgggctc agaatgatac cgagcagaca cgtattattt tgttctgtga tatagaaagg
     1381 ccgatgaagt ggcggtggtc acaaaaaata aataggtgga taggtggggt gctaatgtca
     1441 gcggcagcat cacccaacga taataatgac aggactggtg ctataaatca tttatttaaa
     1501 tatgttaatg ctattcgtga tgcaggtcag aagctaaaaa aaaggaatcg aaaactatat
     1561 tatttgatta aaaatggagt tatttctttg atttttgttt ggcttgctgt ctcagtgttt
     1621 agatggctgt gatttgtttt tttctattga gtcatacata aatttgggtc ttctatgctc
     1681 aatgtatggt tatagataaa aatcgcctgc taaaaaattc tattgaaatc tttttagtaa
     1741 gaaaggcccg aatctgacgc tcagcgggcc tttcgtgaag aagtgcggca gtagacagat
     1801 ttcatctgat cacgttcatg tgctgctgtg caattaagcg tttctttcgg agctttcacg
     1861 gtatttcctt cacgaaaccg gccgccattc actcttgtgc atagccatgc agccgcgcag
     1921 aaattatcag gggcgttttt cagggtttgg gccggggaaa ggtgccggaa acggccctca
     1981 ggccgcagaa acgcgctgag gggctctcag ggggtgcggc caagaagcgt tatggtaaat
     2041 acatattatg gttatttatt aggagtaact ctgggagagt gggctatggg atcagatgtt
     2101 caatggcaga tagataatgg ttatttggcg caagttatgg gggatttttc gacagagtcg
     2161 cctagctttg aaacgccaat gtttgggcga tattttgata cctttcaagc tgctatacat
     2221 gttgcaaaag ctttcccagg gagtacgttg cagccatcag agtacgaaga taaaactggg
     2281 atgctaatac gtcattatta tgtggattgt catccgtatt atgatcgact ccctcctgaa
     2341 aaaagcgata attttcagta tgatgagtac ccttctgcta ttcatgtggc tgcgtgtatt
     2401 gatcaagcta gcaaagaact cgacgaacta ttgtttggtg gtggatatgg ggaaaatggt
     2461 gaggggcatg caagcccgat aattttatat tagtgttagg ggcggaggga tattatccaa
     2521 tttgtaaaat gatcttcttg agatcgtttt gctctgcgcg taatctcttg ctctgaaaac
     2581 gaaaaaacca cctggggagg tggtttttcg aaggttcagt aagttgggga acttctgaac
     2641 cgtggtaaca gtcttgtgcg agacgtgtca ccaaatctgt ccttccagtg tagccgtagt
     2701 ccggccacca cttcaagaac tctcgataca tctctcgcac atcctgctta ccagtggcta
     2761 ccgccaatgg cgattttgtc gtgtctttcc gggttggact caagttgata gttaccggaa
     2821 tgggcgtggt agtcggactg aacggggggg tcgtgcatac agtccagctt ggagcgaact
     2881 gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccaca taacagcgga
     2941 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagct accaggggga
     3001 aacgcctggg atctttatag tcctgtcggg tttcgccgcc actgatttga gcgtcatatt
     3061 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gcaacgccgc atccttttcc
     3121 cagcccttcc tgatttgagc gccatctctt tatcccgcca ttcgtgagcc ggtaacgccc
     3181 gccgcagtct cacggcagga gcgtagcgac cgagtgagcg aggaggcgtc atttcatcgt
     3241 ccccagcatt tcgcacttac gccccacaga cgcatttttt acctgccaca tgaagcactt
     3301 ccctgaattc ccacttcgat ttaccatatt aagccagtat acactccgct agcgctacgt
     3361 gactggttca gggctgcgcc ccgaaacccg ctaaaaccac tgacgcgcct gcggcttgtc
     3421 caacaccgcg ccgaccggga aaagatttcc cgcccgtccc ggcgttatca ggaggccgga
     3481 ttggcagaca aacgcagcaa aatgctcacg atgtgggtga ccgaggatga gcaccggcgt
     3541 ctgctggagc gctgcgacgg taaacagctt gcagcctgga tgcgccagac gtgcctggac
     3601 gagaaacccg cccgcgccgg caagcttccc tcgatctcgc cggcgctgct tcgtcagctt
     3661 gccggcatgg ggaacaacct taaccagatt gcccggcagg ttaacgccgg tggtggcagc
     3721 gggcacgacc gcgtgcaggt cgtcgccgcg ctgatggcca tagatgccgg actcgagcgg
     3781 ctgcggcatg ccgtgctgga aaagggtgca gacgatgatc gttaagtttc acccgcgagg
     3841 gcgcggcggt ggcgccggtc cggtggatta tctgctgggg aaagaccgac agcgcgaggg
     3901 cgccaccgtt ctgcagggga agccggaaga agtcagggag cttatcgacg cctcacccta
     3961 cgcaaaaaag tacacatccg gcgtcctgtc ctttgccgaa aaggatttac cgcccgggca
     4021 gcgcgaaaag ctgatggcga gctttgaacg ggttctgatg cccggactcg acaaagacca
     4081 gtacagcgtg ctgtgggttg agcaccagga taaggggcgg ctggagctga acttcctgat
     4141 cccgaacacg gagctgctga ctggcaagcg tctccagccg tattacgacc gcgccgaccg
     4201 tccgcgcatt gatgcctggc agaccatcgt gaacggcagg ctggggctgc atgacccgaa
     4261 cgcgcctgaa aaccggcggg cgctggtcac gccgtccgcg ctgccggaaa cgaagcagga
     4321 agccgctcag gcaattacac ggggtttact ggccttggcc tcgtccgggg agctgaaaac
     4381 ccgccaggac gtcactgagg cgctggaaag cgcaggtttt gaggtggtac gcaccacaaa
     4441 aagcagcatc agcattgccg acccggacgg ggggcgaaac atccgactga agggagccat
     4501 ctatgaacag tcttttaacg ctggcgaagg acttagagca gaaatcgaaa gcgcagcagc
     4561 agagtaccgg cgagatgctg aaagccgcat tcagcgagca cgagaagtct gtcagagcgg
     4621 aactgagcga aagcgagaag agaatcagcg ccgccatcct cgaccacgac cggaagctgt
     4681 cctcagccat gagccagcgt acgaaaggga tgctgcgcat ggtcagccag acgtggctga
     4741 ccatcgtcct ggtctccgtg
//
