LOCUS       NZ_AP025882             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80156 plasmid pFJMB80156_3,
            complete sequence.
ACCESSION   NZ_AP025882
VERSION     NZ_AP025882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490880
            Assembly: GCF_023735755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 200x
            Sequencing Technology             :: NextSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735755.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:13:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,272
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 26 of 95
            Pseudo Genes (incomplete)         :: 72 of 95
            Pseudo Genes (internal stop)      :: 12 of 95
            Pseudo Genes (multiple problems)  :: 13 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025882.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80156"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80156_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-05-21"
     gene            complement(253..480)
                     /locus_tag="NGJ59_RS26305"
                     /old_locus_tag="FJMB80156_51690"
     CDS             complement(253..480)
                     /locus_tag="NGJ59_RS26305"
                     /old_locus_tag="FJMB80156_51690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(908..1051)
                     /locus_tag="NGJ59_RS26310"
     CDS             complement(908..1051)
                     /locus_tag="NGJ59_RS26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(2212..2397)
                     /locus_tag="NGJ59_RS26315"
                     /old_locus_tag="FJMB80156_51720"
     CDS             complement(2212..2397)
                     /locus_tag="NGJ59_RS26315"
                     /old_locus_tag="FJMB80156_51720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2086..2165
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(887..1447)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ggtggtttgt tgtggttttt ggtcatggtt ccgtcagaaa agcgctgagt gcgtctgagg
       61 cggtgcgggc aacggatgtt atggtaaatt cttggggggc ttgattataa aaaccgcact
      121 tcctctaatg tcttgtgctt tttagacaac cctggaagct tttagctttg gtggtgcaat
      181 ttgctgggat tttggcggag aaccccaggt aaagaaaaag gccacattag cggccttttt
      241 cggagaagat gctcagtgcg gaatttcgga gacttcttta acgtcggttt tattgatctg
      301 ctgtttgaca ccattcgcgt ctgtatagcc aagcaggcct gagtctgatt ctttaggttt
      361 gccgtcgctg acaatagtgc gcccgtcatt ggtgtgtatg gcatagcttg tgcgcgtgca
      421 gccggtgagg gctgaaagcg caactgccgc tgcgaacacg gaaacaaaaa tctttttcaa
      481 agccagctcc ttttatccat atcagttttg gtcactaacc atctaactat aggtcatttt
      541 ttatgtgaaa agaggcttga tggtgggctg catatcgagt taggtgcgag gtgttatggt
      601 taaagtgtgg tgtctggcgg ggagggggca gtgcgggctt atatctgatg taatccgacc
      661 gatacatata ccaagatgat ttaagtattc ctctttgtga ggttggatgt ctaatttata
      721 aaaggatctt cttgagatcc ttttttttcg cgcgtaatct cttgccctgt aaacgaaaaa
      781 accacccggc aaggtggttt ttcgaaggtt cagcaaaccg gcaagtttct gaaccgtggt
      841 aacagggtgt acaagaccgc tgccaccaaa tttgtccttc tagtgtagcc gcagtcaggc
      901 gtcaacttca agaactctgc accagtaatc ttgtataccc cttaccagtg gccgctgcca
      961 gtggcgtttt tacgtgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc
     1021 agcagtcgga ctgaacgggg ggttcgtgca tacagtccat cctggagcga actgccttcc
     1081 cggaaccgag tgtcaggcgt ggaatgagaa accgcggcca taacagcgga gtgacaccgg
     1141 taaaccgaaa ggcaggaatg cgggggagca cgagggagcc atcaggggga aacgcctggt
     1201 atctttaagc cgcatcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctc
     1261 gtcagggggg cggagcctat ggaaaaacgg cttcggcgcg gccttatgcc ttcttcgcta
     1321 agtatcttcc tggcatcccc tagctggcat cccctaggaa atttctgctc cacccgtaag
     1381 cccgtaccgc tcgccgcagc cgaacgaccg agcgcagcga gtgagtgagc gaggaagcgg
     1441 aatatatcct gtattacatt ttctcctgac gcgttttctt tcattttctg cgcctgtctt
     1501 atgtagcagt tatgctatgc gttactgcgg tgttacattt tagccagtat acactccgct
     1561 agcgctgagt gactggtccg gcgctgcgcc cggaacccgc ctgtaccggt tcagcagccg
     1621 ttccgggctg actgcaattt ttttattttc ctgcccgcta ccctgtaaac ctttcttctg
     1681 cgttgccgtt aacaggaggt aagccatgtt tggtttttct tccggagacc tgatgactct
     1741 tgatgatgag cgacacctgt ttgtgtctgt tgtttcgggc tggctccggc gtgccggggg
     1801 gatgccggag ccatgatgct ggatgcatac cggcagattt tgtctgaaac tgaaccggcc
     1861 gtaaggacgg tcatgctcga atttcttgaa tccgttcgca ttcattacat ttcgtcctga
     1921 gcattttctg tatttctgtc catacttaaa cgaggcggcc gtcacggacg atggtcgtcg
     1981 ttgcatgatg ctgttatgga tgagtgaaag taatctaatc taacttgact taccttccgg
     2041 gcagctcccc ggtttttttt tgcctgttcc cggggaacca ctcaggacag gatgtgtatt
     2101 tgtaataccg cgcacgcgcc ggcatcacaa atacccaggt cagggctgcg ccccgacacc
     2161 ccgccatcgc ctcacggccg cgcactgaat tgcgcacccg ttcaccgatt ttcacgcatc
     2221 ttcctcgcct gtctgtgcgt tcagcatggt gtaaagtgct tcggcctgat cgtgcaggtg
     2281 ctcacagcag ccggcagcct catccagatc catctgttcg agccgctcga ggaggaggag
     2341 tgtctgtgag cggataaatt tggccatgtt gagggcggcg gattcctgtc tggtcactgg
     2401 tgattttccc cgtctggtga tgatttttgc gtgaaaagag catgccgtca ttctggtgca
     2461 tagtcatgca tgccgttaaa atttaacagg agcgtttctg gcgggttccg g
//
