LOCUS       NZ_AP025881             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80156 plasmid pFJMB80156_2,
            complete sequence.
ACCESSION   NZ_AP025881
VERSION     NZ_AP025881.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490880
            Assembly: GCF_023735755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 200x
            Sequencing Technology             :: NextSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735755.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:13:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,272
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 26 of 95
            Pseudo Genes (incomplete)         :: 72 of 95
            Pseudo Genes (internal stop)      :: 12 of 95
            Pseudo Genes (multiple problems)  :: 13 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025881.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80156"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80156_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2018-05-21"
     gene            138..461
                     /locus_tag="NGJ59_RS26270"
                     /old_locus_tag="FJMB80156_51650"
     CDS             138..461
                     /locus_tag="NGJ59_RS26270"
                     /old_locus_tag="FJMB80156_51650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            670..>1104
                     /locus_tag="NGJ59_RS26275"
                     /pseudo=""
     CDS             670..>1104
                     /locus_tag="NGJ59_RS26275"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1139..1624
                     /locus_tag="NGJ59_RS26280"
                     /old_locus_tag="FJMB80156_51660"
     CDS             1139..1624
                     /locus_tag="NGJ59_RS26280"
                     /old_locus_tag="FJMB80156_51660"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1628..1843
                     /locus_tag="NGJ59_RS26285"
                     /old_locus_tag="FJMB80156_51670"
     CDS             1628..1843
                     /locus_tag="NGJ59_RS26285"
                     /old_locus_tag="FJMB80156_51670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2114..3028
                     /gene="lpxO"
                     /locus_tag="NGJ59_RS26290"
     CDS             2114..3028
                     /gene="lpxO"
                     /locus_tag="NGJ59_RS26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            3428..3889
                     /locus_tag="NGJ59_RS26295"
     CDS             3428..3889
                     /locus_tag="NGJ59_RS26295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(4144..4254)
                     /locus_tag="NGJ59_RS26300"
     CDS             complement(4144..4254)
                     /locus_tag="NGJ59_RS26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     Typing          4611..4760
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.211"
                     /coverage="86.0"
                     /evalue="7.700000000000001e-33"
     Typing          complement(4145..4628)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          452..1941
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggttcagggc tgcgccccga aacccgctaa aaccactgac gcgcctgcgg cttgtccaac
       61 accgcgccga ccgggaaaag atttcccgcc cgtcccggcg ttatcaggag gccggattgg
      121 cagacaaacg cagcaaaatg ctcacgatgt gggtgaccga ggatgagcac cggcgtctgc
      181 tggagcgctg cgacggtaaa cagcttgcag cctggatgcg ccagacgtgc ctggacgaga
      241 aacccgcccg cgccggcaag cttccctcga tctcgccggc gctgcttcgt cagcttgccg
      301 gcatggggaa caaccttaac cagattgccc ggcaggttaa cgccggtggt ggcagcgggc
      361 acgaccgcgt gcaggtcgtc gccgcgctga tggccataga tgccggactc gagcggctgc
      421 ggcatgccgt gctggaaaag ggtgcagacg atgatcgtta agtttcaccc gcgagggcgc
      481 ggcggtggcg ccggtccggt ggattatctg ctggggaaag accgacagcg cgagggcgcc
      541 accgttctgc aggggaagcc ggaagaagtc agggagctta tcgacgcctc accctacgca
      601 aaaaagtaca catccggcgt cctgtccttt gccgaaaagg atttaccgcc cgggcagcgc
      661 gaaaagctga tggcgagctt tgaacgggtt ctgatgcccg gactcgacaa agaccagtac
      721 agcgtgctgt gggttgagca ccaggataag gggcggctgg agctgaactt cctgatcccg
      781 aacacggagc tgctgactgg caagcgtctc cagccgtatt acgaccgcgc cgaccgtccg
      841 cgcattgatg cctggcagac catcgtgaac ggcaggctgg ggctgcatga cccgaacgcg
      901 cctgaaaacc ggcgggcgct ggtcacgccg tccgcgctgc cggaaacgaa gcaggaagcc
      961 gctcaggcaa ttacacgggg tttactggcc ttggcctcgt ccggggagct gaaaacccgc
     1021 caggacgtca ctgaggcgct ggaaagcgca ggttttgagg tggtacgcac cacaaaaagc
     1081 agcatcagca ttgccgaccc ggacgggggg cgaaacatcc gactgaaggg agccatctat
     1141 gaacagtctt ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagag
     1201 taccggcgag atgctgaaag ccgcattcag cgagcacgag aagtctgtca gagcggaact
     1261 gagcgaaagc gagaagagaa tcagcgccgc catcctcgac cacgaccgga agctgtcctc
     1321 agccatgagc cagcgtacga aagggatgct gcgcatggtc agccagacgt ggctgaccat
     1381 cgtcctggtc tccgtgctgc tgatcgcctc gaacgcggcc attctgtggt ggcagagtca
     1441 gcagatactc gacaattacg tgagcatccg ggagcagaag agcacgcagg cgatgctgtc
     1501 cgagcggaac agcggcgtgc agctctcgac ctgcggcgag cagagacgcc gctgcgtgag
     1561 ggtgaacccg gaagcgggac ggttcggaga ggattcgagc tggatgatac tggcggggaa
     1621 atagcacatg acagagctgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga
     1681 ctactcgaaa aggctggacg agtgggagag cgccttcgcg gaatggcgga cgatgtctgg
     1741 tcttatgcaa cgggagaacg cggcgctgag cgagcgcgtc acgggcttga gcaggcaggt
     1801 gcagagtttg agcgatcagc tgcgccggtt gtcgagcggc tgaatgccgt cgagctgcag
     1861 caggtgcggg aggcgcggca tcagaaagcg ctggagctcg aacgatcaca gcgacagcgg
     1921 gtatatcacg gtccgtccct ttgattcatt tcagcataat tcctgctgaa atcctctctg
     1981 cctgtgtagt agatatgttc tggtgaagct tcgattatac tattgataaa tagcatttag
     2041 attataattt aagtgcattc attatgttat ctttaagaga tttttattat tggcgtgaac
     2101 aaggaatata cacatgctgt cacttattat tatttttgtt tttcttatca gtgtattgta
     2161 tgcgcattct cgcggtaaaa taaaacaaaa gttttccagg cagttatttg atcattcaac
     2221 atttatggct ccgataaata tgtttatgac aggcttttcg aagctgccgt caaagcaggc
     2281 atggtttccc ccagaaacat ttcctgaact tcaaccatta attgataact ggcaggttat
     2341 aagagacgaa gctgttaatt tgcaagggca cattaaatca tctgaaaaaa ataatgatgc
     2401 aggatttaat acatttttta agcgcggttg gaagcgcttt tacctcaagt ggtatggaga
     2461 tgctcatcca tcagcggagc ggctttgccc agtaacaacc caactggtta gctctattcc
     2521 ctgtattaaa gcagcgatgt ttgctgaatt gccttcagga agccatttag gaaagcatcg
     2581 tgacccttat gccggttcag ttcgttatca tttaggatta tcaacaccaa atgatgaacg
     2641 gtgttttatt gaagttgatg gtgtacgccg cagttggcat gatggtgaag tgataatttt
     2701 tgacgaaact tttgttcact gggctcagaa tgataccgag cagacacgta ttattttgtt
     2761 ctgtgatata gaaaggccga tgaagtggcg gtggtcacaa aaaataaata ggtggatagg
     2821 tggggtgcta atgtcagcgg cagcatcacc caacgataat aatgacagga ctggtgctat
     2881 aaatcattta tttaaatatg ttaatgctat tcgtgatgca ggtcagaagc taaaaaaaag
     2941 gaatcgaaaa ctatattatt tgattaaaaa tggagttatt tctttgattt ttgtttggct
     3001 tgctgtctca gtgtttagat ggctgtgatt tgtttttttc tattgagtca tacataaatt
     3061 tgggtcttct atgctcaatg tatggttata gataaaaatc gcctgctaaa aaattctatt
     3121 gaaatctttt tagtaagaaa ggcccgaatc tgacgctcag cgggcctttc gtgaagaagt
     3181 gcggcagtag acagatttca tctgatcacg ttcatgtgct gctgtgcaat taagcgtttc
     3241 tttcggagct ttcacggtat ttccttcacg aaaccggccg ccattcactc ttgtgcatag
     3301 ccatgcagcc gcgcagaaat tatcaggggc gtttttcagg gtttgggccg gggaaaggtg
     3361 ccggaaacgg ccctcaggcc gcagaaacgc gctgaggggc tctcaggggg tgcggccaag
     3421 aagcgttatg gtaaatacat attatggtta tttattagga gtaactctgg gagagtgggc
     3481 tatgggatca gatgttcaat ggcagataga taatggttat ttggcgcaag ttatggggga
     3541 tttttcgaca gagtcgccta gctttgaaac gccaatgttt gggcgatatt ttgatacctt
     3601 tcaagctgct atacatgttg caaaagcttt cccagggagt acgttgcagc catcagagta
     3661 cgaagataaa actgggatgc taatacgtca ttattatgtg gattgtcatc cgtattatga
     3721 tcgactccct cctgaaaaaa gcgataattt tcagtatgat gagtaccctt ctgctattca
     3781 tgtggctgcg tgtattgatc aagctagcaa agaactcgac gaactattgt ttggtggtgg
     3841 atatggggaa aatggtgagg ggcatgcaag cccgataatt ttatattagt gttaggggcg
     3901 gagggatatt atccaatttg taaaatgatc ttcttgagat cgttttgctc tgcgcgtaat
     3961 ctcttgctct gaaaacgaaa aaaccacctg gggaggtggt ttttcgaagg ttcagtaagt
     4021 tggggaactt ctgaaccgtg gtaacagtct tgtgcgagac gtgtcaccaa atctgtcctt
     4081 ccagtgtagc cgtagtccgg ccaccacttc aagaactctc gatacatctc tcgcacatcc
     4141 tgcttaccag tggctaccgc caatggcgat tttgtcgtgt ctttccgggt tggactcaag
     4201 ttgatagtta ccggaatggg cgtggtagtc ggactgaacg ggggggtcgt gcatacagtc
     4261 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
     4321 gccacataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
     4381 ggagctacca gggggaaacg cctgggatct ttatagtcct gtcgggtttc gccgccactg
     4441 atttgagcgt catatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggcaa
     4501 cgccgcatcc ttttcccagc ccttcctgat ttgagcgcca tctctttatc ccgccattcg
     4561 tgagccggta acgcccgccg cagtctcacg gcaggagcgt agcgaccgag tgagcgagga
     4621 ggcgtcattt catcgtcccc agcatttcgc acttacgccc cacagacgca ttttttacct
     4681 gccacatgaa gcacttccct gaattcccac ttcgatttac catattaagc cagtatacac
     4741 tccgctagcg ctacgtgact
//
