LOCUS       NZ_AP025878             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80153 plasmid pFJMB80153_3,
            complete sequence.
ACCESSION   NZ_AP025878
VERSION     NZ_AP025878.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490879
            Assembly: GCF_023735735.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 96x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735735.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:12:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,313
            CDSs (total)                      :: 5,198
            Genes (coding)                    :: 5,102
            CDSs (with protein)               :: 5,102
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 25 of 96
            Pseudo Genes (incomplete)         :: 74 of 96
            Pseudo Genes (internal stop)      :: 13 of 96
            Pseudo Genes (multiple problems)  :: 14 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025878.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80153"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80153_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-07-29"
     gene            complement(join(2453..2511,1..169))
                     /locus_tag="NGJ46_RS26515"
     CDS             complement(join(2453..2511,1..169))
                     /locus_tag="NGJ46_RS26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(597..740)
                     /locus_tag="NGJ46_RS26520"
                     /old_locus_tag="FJMB80153_52230"
     CDS             complement(597..740)
                     /locus_tag="NGJ46_RS26520"
                     /old_locus_tag="FJMB80153_52230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(1901..2086)
                     /locus_tag="NGJ46_RS26525"
                     /old_locus_tag="FJMB80153_52250"
     CDS             complement(1901..2086)
                     /locus_tag="NGJ46_RS26525"
                     /old_locus_tag="FJMB80153_52250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1775..1854
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(576..1136)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 cattcgcgtc tgtatagcca agcaggcctg agtctgattc tttaggtttg ccgtcgctga
       61 caatagtgcg cccgtcattg gtgtgtatgg catagcttgt gcgcgtgcag ccggtgaggg
      121 ctgaaagcgc aactgccgct gcgaacacgg aaacaaaaat ctttttcaaa gccagctcct
      181 tttatccata tcagttttgg tcactaacca tctaactata ggtcattttt tatgtgaaaa
      241 gaggcttgat ggtgggctgc atatcgagtt aggtgcgagg tgttatggtt aaagtgtggt
      301 gtctggcggg gagggggcag tgcgggctta tatctgatgt aatccgaccg atacatatac
      361 caagatgatt taagtattcc tctttgtgag gttggatgtc taatttataa aaggatcttc
      421 ttgagatcct tttttttcgc gcgtaatctc ttgccctgta aacgaaaaaa ccacccggca
      481 aggtggtttt tcgaaggttc agcaaaccgg caagtttctg aaccgtggta acagggtgta
      541 caagaccgct gccaccaaat ttgtccttct agtgtagccg cagtcaggcg tcaacttcaa
      601 gaactctgca ccagtaatct tgtatacccc ttaccagtgg ccgctgccag tggcgttttt
      661 acgtgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcagtcggac
      721 tgaacggggg gttcgtgcat acagtccatc ctggagcgaa ctgccttccc ggaaccgagt
      781 gtcaggcgtg gaatgagaaa ccgcggccat aacagcggag tgacaccggt aaaccgaaag
      841 gcaggaatgc gggggagcac gagggagcca tcagggggaa acgcctggta tctttaagcc
      901 gcatcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgctcg tcaggggggc
      961 ggagcctatg gaaaaacggc ttcggcgcgg ccttatgcct tcttcgctaa gtatcttcct
     1021 ggcatcccct agctggcatc ccctaggaaa tttctgctcc acccgtaagc ccgtaccgct
     1081 cgccgcagcc gaacgaccga gcgcagcgag tgagtgagcg aggaagcgga atatatcctg
     1141 tattacattt tctcctgacg cgttttcttt cattttctgc gcctgtctta tgtagcagtt
     1201 atgctatgcg ttactgcggt gttacatttt agccagtata cactccgcta gcgctgagtg
     1261 actggtccgg cgctgcgccc ggaacccgcc tgtaccggtt cagcagccgt tccgggctga
     1321 ctgcaatttt tttattttcc tgcccgctac cctgtaaacc tttcttctgc gttgccgtta
     1381 acaggaggta agccatgttt ggtttttctt ccggagacct gatgactctt gatgatgagc
     1441 gacacctgtt tgtgtctgtt gtttcgggct ggctccggcg tgccgggggg atgccggagc
     1501 catgatgctg gatgcatacc ggcagatttt gtctgaaact gaaccggccg taaggacggt
     1561 catgctcgaa tttcttgaat ccgttcgcat tcattacatt tcgtcctgag cattttctgt
     1621 atttctgtcc atacttaaac gaggcggccg tcacggacga tggtcgtcgt tgcatgatgc
     1681 tgttatggat gagtgaaagt aatctaatct aacttgactt accttccggg cagctccccg
     1741 gttttttttt gcctgttccc ggggaaccac tcaggacagg atgtgtattt gtaataccgc
     1801 gcacgcgccg gcatcacaaa tacccaggtc agggctgcgc cccgacaccc cgccatcgcc
     1861 tcacggccgc gcactgaatt gcgcacccgt tcaccgattt tcacgcatct tcctcgcctg
     1921 tctgtgcgtt cagcatggtg taaagtgctt cggcctgatc gtgcaggtgc tcacagcagc
     1981 cggcagcctc atccagatcc atctgttcga gccgctcgag gaggaggagt gtctgtgagc
     2041 ggataaattt ggccatgttg agggcggcgg attcctgtct ggtcactggt gattttcccc
     2101 gtctggtgat gatttttgcg tgaaaagagc atgccgtcat tctggtgcat agtcatgcat
     2161 gccgttaaaa tttaacagga gcgtttctgg cgggttccgg ggtggtttgt tgtggttttt
     2221 ggtcatggtt ccgtcagaaa agcgctgagt gcgtctgagg cggtgcgggc aacggatgtt
     2281 atggtaaatt cttggggggc ttgattataa aaaccgcact tcctctaatg tcttgtgctt
     2341 tttagacaac cctggaagct tttagctttg gtggtgcaat ttgctgggat tttggcggag
     2401 aaccccaggt aaagaaaaag gccacattag cggccttttt cggagaagat gctcagtgcg
     2461 gaatttcgga gacttcttta acgtcggttt tattgatctg ctgtttgaca c
//
