LOCUS       NZ_AP025877             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80153 plasmid pFJMB80153_2,
            complete sequence.
ACCESSION   NZ_AP025877
VERSION     NZ_AP025877.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490879
            Assembly: GCF_023735735.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 96x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735735.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:12:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,313
            CDSs (total)                      :: 5,198
            Genes (coding)                    :: 5,102
            CDSs (with protein)               :: 5,102
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 25 of 96
            Pseudo Genes (incomplete)         :: 74 of 96
            Pseudo Genes (internal stop)      :: 13 of 96
            Pseudo Genes (multiple problems)  :: 14 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025877.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80153"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80153_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-07-29"
     gene            join(4360..4760,1..61)
                     /locus_tag="NGJ46_RS26480"
     CDS             join(4360..4760,1..61)
                     /locus_tag="NGJ46_RS26480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(316..426)
                     /locus_tag="NGJ46_RS26485"
     CDS             complement(316..426)
                     /locus_tag="NGJ46_RS26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            1070..1393
                     /locus_tag="NGJ46_RS26490"
                     /old_locus_tag="FJMB80153_52200"
     CDS             1070..1393
                     /locus_tag="NGJ46_RS26490"
                     /old_locus_tag="FJMB80153_52200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1602..>2036
                     /locus_tag="NGJ46_RS26495"
                     /pseudo=""
     CDS             1602..>2036
                     /locus_tag="NGJ46_RS26495"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2071..2556
                     /locus_tag="NGJ46_RS26500"
                     /old_locus_tag="FJMB80153_52210"
     CDS             2071..2556
                     /locus_tag="NGJ46_RS26500"
                     /old_locus_tag="FJMB80153_52210"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2560..2775
                     /locus_tag="NGJ46_RS26505"
                     /old_locus_tag="FJMB80153_52220"
     CDS             2560..2775
                     /locus_tag="NGJ46_RS26505"
                     /old_locus_tag="FJMB80153_52220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3046..3960
                     /gene="lpxO"
                     /locus_tag="NGJ46_RS26510"
     CDS             3046..3960
                     /gene="lpxO"
                     /locus_tag="NGJ46_RS26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     Typing          783..966
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(317..800)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          1384..2873
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttggtggt ggatatgggg aaaatggtga ggggcatgca agcccgataa ttttatatta
       61 gtgttagggg cggagggata ttatccaatt tgtaaaatga tcttcttgag atcgttttgc
      121 tctgcgcgta atctcttgct ctgaaaacga aaaaaccacc tggggaggtg gtttttcgaa
      181 ggttcagtaa gttggggaac ttctgaaccg tggtaacagt cttgtgcgag acgtgtcacc
      241 aaatctgtcc ttccagtgta gccgtagtcc ggccaccact tcaagaactc tcgatacatc
      301 tctcgcacat cctgcttacc agtggctacc gccaatggcg attttgtcgt gtctttccgg
      361 gttggactca agttgatagt taccggaatg ggcgtggtag tcggactgaa cgggggggtc
      421 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
      481 gagacaaacg cggccacata acagcggaat gacaccggta aaccgaaagg caggaacagg
      541 agagcgcacg agggagctac cagggggaaa cgcctgggat ctttatagtc ctgtcgggtt
      601 tcgccgccac tgatttgagc gtcatatttc gtgatgcttg tcaggggggc ggagcctatg
      661 gaaaaacggc aacgccgcat ccttttccca gcccttcctg atttgagcgc catctcttta
      721 tcccgccatt cgtgagccgg taacgcccgc cgcagtctca cggcaggagc gtagcgaccg
      781 agtgagcgag gaggcgtcat ttcatcgtcc ccagcatttc gcacttacgc cccacagacg
      841 cattttttac ctgccacatg aagcacttcc ctgaattccc acttcgattt accatattaa
      901 gccagtatac actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgct
      961 aaaaccactg acgcgcctgc ggcttgtcca acaccgcgcc gaccgggaaa agatttcccg
     1021 cccgtcccgg cgttatcagg aggccggatt ggcagacaaa cgcagcaaaa tgctcacgat
     1081 gtgggtgacc gaggatgagc accggcgtct gctggagcgc tgcgacggta aacagcttgc
     1141 agcctggatg cgccagacgt gcctggacga gaaacccgcc cgcgccggca agcttccctc
     1201 gatctcgccg gcgctgcttc gtcagcttgc cggcatgggg aacaacctta accagattgc
     1261 ccggcaggtt aacgccggtg gtggcagcgg gcacgaccgc gtgcaggtcg tcgccgcgct
     1321 gatggccata gatgccggac tcgagcggct gcggcatgcc gtgctggaaa agggtgcaga
     1381 cgatgatcgt taagtttcac ccgcgagggc gcggcggtgg cgccggtccg gtggattatc
     1441 tgctggggaa agaccgacag cgcgagggcg ccaccgttct gcaggggaag ccggaagaag
     1501 tcagggagct tatcgacgcc tcaccctacg caaaaaagta cacatccggc gtcctgtcct
     1561 ttgccgaaaa ggatttaccg cccgggcagc gcgaaaagct gatggcgagc tttgaacggg
     1621 ttctgatgcc cggactcgac aaagaccagt acagcgtgct gtgggttgag caccaggata
     1681 aggggcggct ggagctgaac ttcctgatcc cgaacacgga gctgctgact ggcaagcgtc
     1741 tccagccgta ttacgaccgc gccgaccgtc cgcgcattga tgcctggcag accatcgtga
     1801 acggcaggct ggggctgcat gacccgaacg cgcctgaaaa ccggcgggcg ctggtcacgc
     1861 cgtccgcgct gccggaaacg aagcaggaag ccgctcaggc aattacacgg ggtttactgg
     1921 ccttggcctc gtccggggag ctgaaaaccc gccaggacgt cactgaggcg ctggaaagcg
     1981 caggttttga ggtggtacgc accacaaaaa gcagcatcag cattgccgac ccggacgggg
     2041 ggcgaaacat ccgactgaag ggagccatct atgaacagtc ttttaacgct ggcgaaggac
     2101 ttagagcaga aatcgaaagc gcagcagcag agtaccggcg agatgctgaa agccgcattc
     2161 agcgagcacg agaagtctgt cagagcggaa ctgagcgaaa gcgagaagag aatcagcgcc
     2221 gccatcctcg accacgaccg gaagctgtcc tcagccatga gccagcgtac gaaagggatg
     2281 ctgcgcatgg tcagccagac gtggctgacc atcgtcctgg tctccgtgct gctgatcgcc
     2341 tcgaacgcgg ccattctgtg gtggcagagt cagcagatac tcgacaatta cgtgagcatc
     2401 cgggagcaga agagcacgca ggcgatgctg tccgagcgga acagcggcgt gcagctctcg
     2461 acctgcggcg agcagagacg ccgctgcgtg agggtgaacc cggaagcggg acggttcgga
     2521 gaggattcga gctggatgat actggcgggg aaatagcaca tgacagagct ggaaaaacag
     2581 ttgctgagcg cattagagca gctacagcag gactactcga aaaggctgga cgagtgggag
     2641 agcgccttcg cggaatggcg gacgatgtct ggtcttatgc aacgggagaa cgcggcgctg
     2701 agcgagcgcg tcacgggctt gagcaggcag gtgcagagtt tgagcgatca gctgcgccgg
     2761 ttgtcgagcg gctgaatgcc gtcgagctgc agcaggtgcg ggaggcgcgg catcagaaag
     2821 cgctggagct cgaacgatca cagcgacagc gggtatatca cggtccgtcc ctttgattca
     2881 tttcagcata attcctgctg aaatcctctc tgcctgtgta gtagatatgt tctggtgaag
     2941 cttcgattat actattgata aatagcattt agattataat ttaagtgcat tcattatgtt
     3001 atctttaaga gatttttatt attggcgtga acaaggaata tacacatgct gtcacttatt
     3061 attatttttg tttttcttat cagtgtattg tatgcgcatt ctcgcggtaa aataaaacaa
     3121 aagttttcca ggcagttatt tgatcattca acatttatgg ctccgataaa tatgtttatg
     3181 acaggctttt cgaagctgcc gtcaaagcag gcatggtttc ccccagaaac atttcctgaa
     3241 cttcaaccat taattgataa ctggcaggtt ataagagacg aagctgttaa tttgcaaggg
     3301 cacattaaat catctgaaaa aaataatgat gcaggattta atacattttt taagcgcggt
     3361 tggaagcgct tttacctcaa gtggtatgga gatgctcatc catcagcgga gcggctttgc
     3421 ccagtaacaa cccaactggt tagctctatt ccctgtatta aagcagcgat gtttgctgaa
     3481 ttgccttcag gaagccattt aggaaagcat cgtgaccctt atgccggttc agttcgttat
     3541 catttaggat tatcaacacc aaatgatgaa cggtgtttta ttgaagttga tggtgtacgc
     3601 cgcagttggc atgatggtga agtgataatt tttgacgaaa cttttgttca ctgggctcag
     3661 aatgataccg agcagacacg tattattttg ttctgtgata tagaaaggcc gatgaagtgg
     3721 cggtggtcac aaaaaataaa taggtggata ggtggggtgc taatgtcagc ggcagcatca
     3781 cccaacgata ataatgacag gactggtgct ataaatcatt tatttaaata tgttaatgct
     3841 attcgtgatg caggtcagaa gctaaaaaaa aggaatcgaa aactatatta tttgattaaa
     3901 aatggagtta tttctttgat ttttgtttgg cttgctgtct cagtgtttag atggctgtga
     3961 tttgtttttt tctattgagt catacataaa tttgggtctt ctatgctcaa tgtatggtta
     4021 tagataaaaa tcgcctgcta aaaaattcta ttgaaatctt tttagtaaga aaggcccgaa
     4081 tctgacgctc agcgggcctt tcgtgaagaa gtgcggcagt agacagattt catctgatca
     4141 cgttcatgtg ctgctgtgca attaagcgtt tctttcggag ctttcacggt atttccttca
     4201 cgaaaccggc cgccattcac tcttgtgcat agccatgcag ccgcgcagaa attatcaggg
     4261 gcgtttttca gggtttgggc cggggaaagg tgccggaaac ggccctcagg ccgcagaaac
     4321 gcgctgaggg gctctcaggg ggtgcggcca agaagcgtta tggtaaatac atattatggt
     4381 tatttattag gagtaactct gggagagtgg gctatgggat cagatgttca atggcagata
     4441 gataatggtt atttggcgca agttatgggg gatttttcga cagagtcgcc tagctttgaa
     4501 acgccaatgt ttgggcgata ttttgatacc tttcaagctg ctatacatgt tgcaaaagct
     4561 ttcccaggga gtacgttgca gccatcagag tacgaagata aaactgggat gctaatacgt
     4621 cattattatg tggattgtca tccgtattat gatcgactcc ctcctgaaaa aagcgataat
     4681 tttcagtatg atgagtaccc ttctgctatt catgtggctg cgtgtattga tcaagctagc
     4741 aaagaactcg acgaactatt
//
