LOCUS       NZ_AP025874             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80152 plasmid pFJMB80152_3,
            complete sequence.
ACCESSION   NZ_AP025874
VERSION     NZ_AP025874.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490878
            Assembly: GCF_023735715.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 115x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735715.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:10:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,292
            CDSs (total)                      :: 5,177
            Genes (coding)                    :: 5,082
            CDSs (with protein)               :: 5,082
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 28 of 95
            Pseudo Genes (incomplete)         :: 71 of 95
            Pseudo Genes (internal stop)      :: 12 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025874.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80152"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80152_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-04-20"
     gene            174..359
                     /locus_tag="NGJ83_RS26420"
                     /old_locus_tag="FJMB80152_51950"
     CDS             174..359
                     /locus_tag="NGJ83_RS26420"
                     /old_locus_tag="FJMB80152_51950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1520..1663
                     /locus_tag="NGJ83_RS26425"
     CDS             1520..1663
                     /locus_tag="NGJ83_RS26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            2091..2318
                     /locus_tag="NGJ83_RS26430"
                     /old_locus_tag="FJMB80152_51980"
     CDS             2091..2318
                     /locus_tag="NGJ83_RS26430"
                     /old_locus_tag="FJMB80152_51980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(407..486)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          1125..1685
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ctcagacgca ctcagcgctt ttctgacgga accatgacca aaaaccacaa caaaccaccc
       61 cggaacccgc cagaaacgct cctgttaaat tttaacggca tgcatgacta tgcaccagaa
      121 tgacggcatg ctcttttcac gcaaaaatca tcaccagacg gggaaaatca ccagtgacca
      181 gacaggaatc cgccgccctc aacatggcca aatttatccg ctcacagaca ctcctcctcc
      241 tcgagcggct cgaacagatg gatctggatg aggctgccgg ctgctgtgag cacctgcacg
      301 atcaggccga agcactttac accatgctga acgcacagac aggcgaggaa gatgcgtgaa
      361 aatcggtgaa cgggtgcgca attcagtgcg cggccgtgag gcgatggcgg ggtgtcgggg
      421 cgcagccctg acctgggtat ttgtgatgcc ggcgcgtgcg cggtattaca aatacacatc
      481 ctgtcctgag tggttccccg ggaacaggca aaaaaaaacc ggggagctgc ccggaaggta
      541 agtcaagtta gattagatta ctttcactca tccataacag catcatgcaa cgacgaccat
      601 cgtccgtgac ggccgcctcg tttaagtatg gacagaaata cagaaaatgc tcaggacgaa
      661 atgtaatgaa tgcgaacgga ttcaagaaat tcgagcatga ccgtccttac ggccggttca
      721 gtttcagaca aaatctgccg gtatgcatcc agcatcatgg ctccggcatc cccccggcac
      781 gccggagcca gcccgaaaca acagacacaa acaggtgtcg ctcatcatca agagtcatca
      841 ggtctccgga agaaaaacca aacatggctt acctcctgtt aacggcaacg cagaagaaag
      901 gtttacaggg tagcgggcag gaaaataaaa aaattgcagt cagcccggaa cggctgctga
      961 accggtacag gcgggttccg ggcgcagcgc cggaccagtc actcagcgct agcggagtgt
     1021 atactggcta aaatgtaaca ccgcagtaac gcatagcata actgctacat aagacaggcg
     1081 cagaaaatga aagaaaacgc gtcaggagaa aatgtaatac aggatatatt ccgcttcctc
     1141 gctcactcac tcgctgcgct cggtcgttcg gctgcggcga gcggtacggg cttacgggtg
     1201 gagcagaaat ttcctagggg atgccagcta ggggatgcca ggaagatact tagcgaagaa
     1261 ggcataaggc cgcgccgaag ccgtttttcc ataggctccg cccccctgac gagcatcacg
     1321 aaatctgacg ctcaaatcag tggtggcgaa acccgatgcg gcttaaagat accaggcgtt
     1381 tccccctgat ggctccctcg tgctcccccg cattcctgcc tttcggttta ccggtgtcac
     1441 tccgctgtta tggccgcggt ttctcattcc acgcctgaca ctcggttccg ggaaggcagt
     1501 tcgctccagg atggactgta tgcacgaacc ccccgttcag tccgactgct gcgccttatc
     1561 cggtaactat cgtcttgagt ccaacccgga aagacacgta aaaacgccac tggcagcggc
     1621 cactggtaag gggtatacaa gattactggt gcagagttct tgaagttgac gcctgactgc
     1681 ggctacacta gaaggacaaa tttggtggca gcggtcttgt acaccctgtt accacggttc
     1741 agaaacttgc cggtttgctg aaccttcgaa aaaccacctt gccgggtggt tttttcgttt
     1801 acagggcaag agattacgcg cgaaaaaaaa ggatctcaag aagatccttt tataaattag
     1861 acatccaacc tcacaaagag gaatacttaa atcatcttgg tatatgtatc ggtcggatta
     1921 catcagatat aagcccgcac tgccccctcc ccgccagaca ccacacttta accataacac
     1981 ctcgcaccta actcgatatg cagcccacca tcaagcctct tttcacataa aaaatgacct
     2041 atagttagat ggttagtgac caaaactgat atggataaaa ggagctggct ttgaaaaaga
     2101 tttttgtttc cgtgttcgca gcggcagttg cgctttcagc cctcaccggc tgcacgcgca
     2161 caagctatgc catacacacc aatgacgggc gcactattgt cagcgacggc aaacctaaag
     2221 aatcagactc aggcctgctt ggctatacag acgcgaatgg tgtcaaacag cagatcaata
     2281 aaaccgacgt taaagaagtc tccgaaattc cgcactgagc atcttctccg aaaaaggccg
     2341 ctaatgtggc ctttttcttt acctggggtt ctccgccaaa atcccagcaa attgcaccac
     2401 caaagctaaa agcttccagg gttgtctaaa aagcacaaga cattagagga agtgcggttt
     2461 ttataatcaa gccccccaag aatttaccat aacatccgtt gcccgcaccg c
//
