LOCUS       NZ_AP025873             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80152 plasmid pFJMB80152_2,
            complete sequence.
ACCESSION   NZ_AP025873
VERSION     NZ_AP025873.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490878
            Assembly: GCF_023735715.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 115x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735715.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:10:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,292
            CDSs (total)                      :: 5,177
            Genes (coding)                    :: 5,082
            CDSs (with protein)               :: 5,082
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 28 of 95
            Pseudo Genes (incomplete)         :: 71 of 95
            Pseudo Genes (internal stop)      :: 12 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025873.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80152"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80152_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-04-20"
     gene            join(4604..4760,1..305)
                     /locus_tag="NGJ83_RS26385"
     CDS             join(4604..4760,1..305)
                     /locus_tag="NGJ83_RS26385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(560..670)
                     /locus_tag="NGJ83_RS26390"
     CDS             complement(560..670)
                     /locus_tag="NGJ83_RS26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            1314..1637
                     /locus_tag="NGJ83_RS26395"
                     /old_locus_tag="FJMB80152_51920"
     CDS             1314..1637
                     /locus_tag="NGJ83_RS26395"
                     /old_locus_tag="FJMB80152_51920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1846..>2280
                     /locus_tag="NGJ83_RS26400"
                     /pseudo=""
     CDS             1846..>2280
                     /locus_tag="NGJ83_RS26400"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2315..2800
                     /locus_tag="NGJ83_RS26405"
                     /old_locus_tag="FJMB80152_51930"
     CDS             2315..2800
                     /locus_tag="NGJ83_RS26405"
                     /old_locus_tag="FJMB80152_51930"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2804..3019
                     /locus_tag="NGJ83_RS26410"
                     /old_locus_tag="FJMB80152_51940"
     CDS             2804..3019
                     /locus_tag="NGJ83_RS26410"
                     /old_locus_tag="FJMB80152_51940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3290..4204
                     /gene="lpxO"
                     /locus_tag="NGJ83_RS26415"
     CDS             3290..4204
                     /gene="lpxO"
                     /locus_tag="NGJ83_RS26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     Typing          1027..1210
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(561..1044)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          1628..3117
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatattttga tacctttcaa gctgctatac atgttgcaaa agctttccca gggagtacgt
       61 tgcagccatc agagtacgaa gataaaactg ggatgctaat acgtcattat tatgtggatt
      121 gtcatccgta ttatgatcga ctccctcctg aaaaaagcga taattttcag tatgatgagt
      181 acccttctgc tattcatgtg gctgcgtgta ttgatcaagc tagcaaagaa ctcgacgaac
      241 tattgtttgg tggtggatat ggggaaaatg gtgaggggca tgcaagcccg ataattttat
      301 attagtgtta ggggcggagg gatattatcc aatttgtaaa atgatcttct tgagatcgtt
      361 ttgctctgcg cgtaatctct tgctctgaaa acgaaaaaac cacctgggga ggtggttttt
      421 cgaaggttca gtaagttggg gaacttctga accgtggtaa cagtcttgtg cgagacgtgt
      481 caccaaatct gtccttccag tgtagccgta gtccggccac cacttcaaga actctcgata
      541 catctctcgc acatcctgct taccagtggc taccgccaat ggcgattttg tcgtgtcttt
      601 ccgggttgga ctcaagttga tagttaccgg aatgggcgtg gtagtcggac tgaacggggg
      661 ggtcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      721 gaatgagaca aacgcggcca cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
      781 caggagagcg cacgagggag ctaccagggg gaaacgcctg ggatctttat agtcctgtcg
      841 ggtttcgccg ccactgattt gagcgtcata tttcgtgatg cttgtcaggg gggcggagcc
      901 tatggaaaaa cggcaacgcc gcatcctttt cccagccctt cctgatttga gcgccatctc
      961 tttatcccgc cattcgtgag ccggtaacgc ccgccgcagt ctcacggcag gagcgtagcg
     1021 accgagtgag cgaggaggcg tcatttcatc gtccccagca tttcgcactt acgccccaca
     1081 gacgcatttt ttacctgcca catgaagcac ttccctgaat tcccacttcg atttaccata
     1141 ttaagccagt atacactccg ctagcgctac gtgactggtt cagggctgcg ccccgaaacc
     1201 cgctaaaacc actgacgcgc ctgcggcttg tccaacaccg cgccgaccgg gaaaagattt
     1261 cccgcccgtc ccggcgttat caggaggccg gattggcaga caaacgcagc aaaatgctca
     1321 cgatgtgggt gaccgaggat gagcaccggc gtctgctgga gcgctgcgac ggtaaacagc
     1381 ttgcagcctg gatgcgccag acgtgcctgg acgagaaacc cgcccgcgcc ggcaagcttc
     1441 cctcgatctc gccggcgctg cttcgtcagc ttgccggcat ggggaacaac cttaaccaga
     1501 ttgcccggca ggttaacgcc ggtggtggca gcgggcacga ccgcgtgcag gtcgtcgccg
     1561 cgctgatggc catagatgcc ggactcgagc ggctgcggca tgccgtgctg gaaaagggtg
     1621 cagacgatga tcgttaagtt tcacccgcga gggcgcggcg gtggcgccgg tccggtggat
     1681 tatctgctgg ggaaagaccg acagcgcgag ggcgccaccg ttctgcaggg gaagccggaa
     1741 gaagtcaggg agcttatcga cgcctcaccc tacgcaaaaa agtacacatc cggcgtcctg
     1801 tcctttgccg aaaaggattt accgcccggg cagcgcgaaa agctgatggc gagctttgaa
     1861 cgggttctga tgcccggact cgacaaagac cagtacagcg tgctgtgggt tgagcaccag
     1921 gataaggggc ggctggagct gaacttcctg atcccgaaca cggagctgct gactggcaag
     1981 cgtctccagc cgtattacga ccgcgccgac cgtccgcgca ttgatgcctg gcagaccatc
     2041 gtgaacggca ggctggggct gcatgacccg aacgcgcctg aaaaccggcg ggcgctggtc
     2101 acgccgtccg cgctgccgga aacgaagcag gaagccgctc aggcaattac acggggttta
     2161 ctggccttgg cctcgtccgg ggagctgaaa acccgccagg acgtcactga ggcgctggaa
     2221 agcgcaggtt ttgaggtggt acgcaccaca aaaagcagca tcagcattgc cgacccggac
     2281 ggggggcgaa acatccgact gaagggagcc atctatgaac agtcttttaa cgctggcgaa
     2341 ggacttagag cagaaatcga aagcgcagca gcagagtacc ggcgagatgc tgaaagccgc
     2401 attcagcgag cacgagaagt ctgtcagagc ggaactgagc gaaagcgaga agagaatcag
     2461 cgccgccatc ctcgaccacg accggaagct gtcctcagcc atgagccagc gtacgaaagg
     2521 gatgctgcgc atggtcagcc agacgtggct gaccatcgtc ctggtctccg tgctgctgat
     2581 cgcctcgaac gcggccattc tgtggtggca gagtcagcag atactcgaca attacgtgag
     2641 catccgggag cagaagagca cgcaggcgat gctgtccgag cggaacagcg gcgtgcagct
     2701 ctcgacctgc ggcgagcaga gacgccgctg cgtgagggtg aacccggaag cgggacggtt
     2761 cggagaggat tcgagctgga tgatactggc ggggaaatag cacatgacag agctggaaaa
     2821 acagttgctg agcgcattag agcagctaca gcaggactac tcgaaaaggc tggacgagtg
     2881 ggagagcgcc ttcgcggaat ggcggacgat gtctggtctt atgcaacggg agaacgcggc
     2941 gctgagcgag cgcgtcacgg gcttgagcag gcaggtgcag agtttgagcg atcagctgcg
     3001 ccggttgtcg agcggctgaa tgccgtcgag ctgcagcagg tgcgggaggc gcggcatcag
     3061 aaagcgctgg agctcgaacg atcacagcga cagcgggtat atcacggtcc gtccctttga
     3121 ttcatttcag cataattcct gctgaaatcc tctctgcctg tgtagtagat atgttctggt
     3181 gaagcttcga ttatactatt gataaatagc atttagatta taatttaagt gcattcatta
     3241 tgttatcttt aagagatttt tattattggc gtgaacaagg aatatacaca tgctgtcact
     3301 tattattatt tttgtttttc ttatcagtgt attgtatgcg cattctcgcg gtaaaataaa
     3361 acaaaagttt tccaggcagt tatttgatca ttcaacattt atggctccga taaatatgtt
     3421 tatgacaggc ttttcgaagc tgccgtcaaa gcaggcatgg tttcccccag aaacatttcc
     3481 tgaacttcaa ccattaattg ataactggca ggttataaga gacgaagctg ttaatttgca
     3541 agggcacatt aaatcatctg aaaaaaataa tgatgcagga tttaatacat tttttaagcg
     3601 cggttggaag cgcttttacc tcaagtggta tggagatgct catccatcag cggagcggct
     3661 ttgcccagta acaacccaac tggttagctc tattccctgt attaaagcag cgatgtttgc
     3721 tgaattgcct tcaggaagcc atttaggaaa gcatcgtgac ccttatgccg gttcagttcg
     3781 ttatcattta ggattatcaa caccaaatga tgaacggtgt tttattgaag ttgatggtgt
     3841 acgccgcagt tggcatgatg gtgaagtgat aatttttgac gaaacttttg ttcactgggc
     3901 tcagaatgat accgagcaga cacgtattat tttgttctgt gatatagaaa ggccgatgaa
     3961 gtggcggtgg tcacaaaaaa taaataggtg gataggtggg gtgctaatgt cagcggcagc
     4021 atcacccaac gataataatg acaggactgg tgctataaat catttattta aatatgttaa
     4081 tgctattcgt gatgcaggtc agaagctaaa aaaaaggaat cgaaaactat attatttgat
     4141 taaaaatgga gttatttctt tgatttttgt ttggcttgct gtctcagtgt ttagatggct
     4201 gtgatttgtt tttttctatt gagtcataca taaatttggg tcttctatgc tcaatgtatg
     4261 gttatagata aaaatcgcct gctaaaaaat tctattgaaa tctttttagt aagaaaggcc
     4321 cgaatctgac gctcagcggg cctttcgtga agaagtgcgg cagtagacag atttcatctg
     4381 atcacgttca tgtgctgctg tgcaattaag cgtttctttc ggagctttca cggtatttcc
     4441 ttcacgaaac cggccgccat tcactcttgt gcatagccat gcagccgcgc agaaattatc
     4501 aggggcgttt ttcagggttt gggccgggga aaggtgccgg aaacggccct caggccgcag
     4561 aaacgcgctg aggggctctc agggggtgcg gccaagaagc gttatggtaa atacatatta
     4621 tggttattta ttaggagtaa ctctgggaga gtgggctatg ggatcagatg ttcaatggca
     4681 gatagataat ggttatttgg cgcaagttat gggggatttt tcgacagagt cgcctagctt
     4741 tgaaacgcca atgtttgggc
//
