LOCUS       NZ_AP025870             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80151 plasmid pFJMB80151_3,
            complete sequence.
ACCESSION   NZ_AP025870
VERSION     NZ_AP025870.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490877
            Assembly: GCF_023735695.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 106x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735695.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,280
            CDSs (total)                      :: 5,165
            Genes (coding)                    :: 5,066
            CDSs (with protein)               :: 5,066
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 30 of 99
            Pseudo Genes (incomplete)         :: 74 of 99
            Pseudo Genes (internal stop)      :: 12 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            Pseudo Genes (short protein)      :: 1 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025870.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80151"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80151_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-04-20"
     gene            complement(210..353)
                     /locus_tag="NGK26_RS26360"
                     /old_locus_tag="FJMB80151_51750"
     CDS             complement(210..353)
                     /locus_tag="NGK26_RS26360"
                     /old_locus_tag="FJMB80151_51750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(1514..1699)
                     /locus_tag="NGK26_RS26365"
                     /old_locus_tag="FJMB80151_51770"
     CDS             complement(1514..1699)
                     /locus_tag="NGK26_RS26365"
                     /old_locus_tag="FJMB80151_51770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2066..2293)
                     /locus_tag="NGK26_RS26370"
                     /old_locus_tag="FJMB80151_51780"
     CDS             complement(2066..2293)
                     /locus_tag="NGK26_RS26370"
                     /old_locus_tag="FJMB80151_51780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1388..1467
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(189..749)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gaggttggat gtctaattta taaaaggatc ttcttgagat cctttttttt cgcgcgtaat
       61 ctcttgccct gtaaacgaaa aaaccacccg gcaaggtggt ttttcgaagg ttcagcaaac
      121 cggcaagttt ctgaaccgtg gtaacagggt gtacaagacc gctgccacca aatttgtcct
      181 tctagtgtag ccgcagtcag gcgtcaactt caagaactct gcaccagtaa tcttgtatac
      241 cccttaccag tggccgctgc cagtggcgtt tttacgtgtc tttccgggtt ggactcaaga
      301 cgatagttac cggataaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc
      361 atcctggagc gaactgcctt cccggaaccg agtgtcaggc gtggaatgag aaaccgcggc
      421 cataacagcg gagtgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag
      481 ccatcagggg gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg
      541 agcgtcagat ttcgtgatgc tcgtcagggg ggcggagcct atggaaaaac ggcttcggcg
      601 cggccttatg ccttcttcgc taagtatctt cctggcatcc cctagctggc atcccctagg
      661 aaatttctgc tccacccgta agcccgtacc gctcgccgca gccgaacgac cgagcgcagc
      721 gagtgagtga gcgaggaagc ggaatatatc ctgtattaca ttttctcctg acgcgttttc
      781 tttcattttc tgcgcctgtc ttatgtagca gttatgctat gcgttactgc ggtgttacat
      841 tttagccagt atacactccg ctagcgctga gtgactggtc cggcgctgcg cccggaaccc
      901 gcctgtaccg gttcagcagc cgttccgggc tgactgcaat ttttttattt tcctgcccgc
      961 taccctgtaa acctttcttc tgcgttgccg ttaacaggag gtaagccatg tttggttttt
     1021 cttccggaga cctgatgact cttgatgatg agcgacacct gtttgtgtct gttgtttcgg
     1081 gctggctccg gcgtgccggg gggatgccgg agccatgatg ctggatgcat accggcagat
     1141 tttgtctgaa actgaaccgg ccgtaaggac ggtcatgctc gaatttcttg aatccgttcg
     1201 cattcattac atttcgtcct gagcattttc tgtatttctg tccatactta aacgaggcgg
     1261 ccgtcacgga cgatggtcgt cgttgcatga tgctgttatg gatgagtgaa agtaatctaa
     1321 tctaacttga cttaccttcc gggcagctcc ccggtttttt tttgcctgtt cccggggaac
     1381 cactcaggac aggatgtgta tttgtaatac cgcgcacgcg ccggcatcac aaatacccag
     1441 gtcagggctg cgccccgaca ccccgccatc gcctcacggc cgcgcactga attgcgcacc
     1501 cgttcaccga ttttcacgca tcttcctcgc ctgtctgtgc gttcagcatg gtgtaaagtg
     1561 cttcggcctg atcgtgcagg tgctcacagc agccggcagc ctcatccaga tccatctgtt
     1621 cgagccgctc gaggaggagg agtgtctgtg agcggataaa tttggccatg ttgagggcgg
     1681 cggattcctg tctggtcact ggtgattttc cccgtctggt gatgattttt gcgtgaaaag
     1741 agcatgccgt cattctggtg catagtcatg catgccgtta aaatttaaca ggagcgtttc
     1801 tggcgggttc cggggtggtt tgttgtggtt tttggtcatg gttccgtcag aaaagcgctg
     1861 agtgcgtctg aggcggtgcg ggcaacggat gttatggtaa attcttgggg ggcttgatta
     1921 taaaaaccgc acttcctcta atgtcttgtg ctttttagac aaccctggaa gcttttagct
     1981 ttggtggtgc aatttgctgg gattttggcg gagaacccca ggtaaagaaa aaggccacat
     2041 tagcggcctt tttcggagaa gatgctcagt gcggaatttc ggagacttct ttaacgtcgg
     2101 ttttattgat ctgctgtttg acaccattcg cgtctgtata gccaagcagg cctgagtctg
     2161 attctttagg tttgccgtcg ctgacaatag tgcgcccgtc attggtgtgt atggcatagc
     2221 ttgtgcgcgt gcagccggtg agggctgaaa gcgcaactgc cgctgcgaac acggaaacaa
     2281 aaatcttttt caaagccagc tccttttatc catatcagtt ttggtcacta accatctaac
     2341 tataggtcat tttttatgtg aaaagaggct tgatggtggg ctgcatatcg agttaggtgc
     2401 gaggtgttat ggttaaagtg tggtgtctgg cggggagggg gcagtgcggg cttatatctg
     2461 atgtaatccg accgatacat ataccaagat gatttaagta ttcctctttg t
//
