LOCUS       NZ_AP025869             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80151 plasmid pFJMB80151_2,
            complete sequence.
ACCESSION   NZ_AP025869
VERSION     NZ_AP025869.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490877
            Assembly: GCF_023735695.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 106x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735695.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,280
            CDSs (total)                      :: 5,165
            Genes (coding)                    :: 5,066
            CDSs (with protein)               :: 5,066
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 30 of 99
            Pseudo Genes (incomplete)         :: 74 of 99
            Pseudo Genes (internal stop)      :: 12 of 99
            Pseudo Genes (multiple problems)  :: 15 of 99
            Pseudo Genes (short protein)      :: 1 of 99
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025869.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80151"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80151_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-04-20"
     gene            complement(229..1143)
                     /gene="lpxO"
                     /locus_tag="NGK26_RS26325"
     CDS             complement(229..1143)
                     /gene="lpxO"
                     /locus_tag="NGK26_RS26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(1414..1629)
                     /locus_tag="NGK26_RS26330"
                     /old_locus_tag="FJMB80151_51710"
     CDS             complement(1414..1629)
                     /locus_tag="NGK26_RS26330"
                     /old_locus_tag="FJMB80151_51710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1633..2118)
                     /locus_tag="NGK26_RS26335"
                     /old_locus_tag="FJMB80151_51720"
     CDS             complement(1633..2118)
                     /locus_tag="NGK26_RS26335"
                     /old_locus_tag="FJMB80151_51720"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2153..2857)
                     /locus_tag="NGK26_RS26440"
                     /pseudo=""
     CDS             complement(<2153..2857)
                     /locus_tag="NGK26_RS26440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2796..3119)
                     /locus_tag="NGK26_RS26345"
                     /old_locus_tag="FJMB80151_51730"
     CDS             complement(2796..3119)
                     /locus_tag="NGK26_RS26345"
                     /old_locus_tag="FJMB80151_51730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3763..3873
                     /locus_tag="NGK26_RS26350"
     CDS             3763..3873
                     /locus_tag="NGK26_RS26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(4128..4589)
                     /locus_tag="NGK26_RS26355"
     CDS             complement(4128..4589)
                     /locus_tag="NGK26_RS26355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     Typing          complement(3221..3407)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          3387..3873
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(1317..2806)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtgaaagct ccgaaagaaa cgcttaattg cacagcagca catgaacgtg atcagatgaa
       61 atctgtctac tgccgcactt cttcacgaaa ggcccgctga gcgtcagatt cgggcctttc
      121 ttactaaaaa gatttcaata gaatttttta gcaggcgatt tttatctata accatacatt
      181 gagcatagaa gacccaaatt tatgtatgac tcaatagaaa aaaacaaatc acagccatct
      241 aaacactgag acagcaagcc aaacaaaaat caaagaaata actccatttt taatcaaata
      301 atatagtttt cgattccttt tttttagctt ctgacctgca tcacgaatag cattaacata
      361 tttaaataaa tgatttatag caccagtcct gtcattatta tcgttgggtg atgctgccgc
      421 tgacattagc accccaccta tccacctatt tattttttgt gaccaccgcc acttcatcgg
      481 cctttctata tcacagaaca aaataatacg tgtctgctcg gtatcattct gagcccagtg
      541 aacaaaagtt tcgtcaaaaa ttatcacttc accatcatgc caactgcggc gtacaccatc
      601 aacttcaata aaacaccgtt catcatttgg tgttgataat cctaaatgat aacgaactga
      661 accggcataa gggtcacgat gctttcctaa atggcttcct gaaggcaatt cagcaaacat
      721 cgctgcttta atacagggaa tagagctaac cagttgggtt gttactgggc aaagccgctc
      781 cgctgatgga tgagcatctc cataccactt gaggtaaaag cgcttccaac cgcgcttaaa
      841 aaatgtatta aatcctgcat cattattttt ttcagatgat ttaatgtgcc cttgcaaatt
      901 aacagcttcg tctcttataa cctgccagtt atcaattaat ggttgaagtt caggaaatgt
      961 ttctggggga aaccatgcct gctttgacgg cagcttcgaa aagcctgtca taaacatatt
     1021 tatcggagcc ataaatgttg aatgatcaaa taactgcctg gaaaactttt gttttatttt
     1081 accgcgagaa tgcgcataca atacactgat aagaaaaaca aaaataataa taagtgacag
     1141 catgtgtata ttccttgttc acgccaataa taaaaatctc ttaaagataa cataatgaat
     1201 gcacttaaat tataatctaa atgctattta tcaatagtat aatcgaagct tcaccagaac
     1261 atatctacta cacaggcaga gaggatttca gcaggaatta tgctgaaatg aatcaaaggg
     1321 acggaccgtg atatacccgc tgtcgctgtg atcgttcgag ctccagcgct ttctgatgcc
     1381 gcgcctcccg cacctgctgc agctcgacgg cattcagccg ctcgacaacc ggcgcagctg
     1441 atcgctcaaa ctctgcacct gcctgctcaa gcccgtgacg cgctcgctca gcgccgcgtt
     1501 ctcccgttgc ataagaccag acatcgtccg ccattccgcg aaggcgctct cccactcgtc
     1561 cagccttttc gagtagtcct gctgtagctg ctctaatgcg ctcagcaact gtttttccag
     1621 ctctgtcatg tgctatttcc ccgccagtat catccagctc gaatcctctc cgaaccgtcc
     1681 cgcttccggg ttcaccctca cgcagcggcg tctctgctcg ccgcaggtcg agagctgcac
     1741 gccgctgttc cgctcggaca gcatcgcctg cgtgctcttc tgctcccgga tgctcacgta
     1801 attgtcgagt atctgctgac tctgccacca cagaatggcc gcgttcgagg cgatcagcag
     1861 cacggagacc aggacgatgg tcagccacgt ctggctgacc atgcgcagca tccctttcgt
     1921 acgctggctc atggctgagg acagcttccg gtcgtggtcg aggatggcgg cgctgattct
     1981 cttctcgctt tcgctcagtt ccgctctgac agacttctcg tgctcgctga atgcggcttt
     2041 cagcatctcg ccggtactct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag
     2101 cgttaaaaga ctgttcatag atggctccct tcagtcggat gtttcgcccc ccgtccgggt
     2161 cggcaatgct gatgctgctt tttgtggtgc gtaccacctc aaaacctgcg ctttccagcg
     2221 cctcagtgac gtcctggcgg gttttcagct ccccggacga ggccaaggcc agtaaacccc
     2281 gtgtaattgc ctgagcggct tcctgcttcg tttccggcag cgcggacggc gtgaccagcg
     2341 cccgccggtt ttcaggcgcg ttcgggtcat gcagccccag cctgccgttc acgatggtct
     2401 gccaggcatc aatgcgcgga cggtcggcgc ggtcgtaata cggctggaga cgcttgccag
     2461 tcagcagctc cgtgttcggg atcaggaagt tcagctccag ccgcccctta tcctggtgct
     2521 caacccacag cacgctgtac tggtctttgt cgagtccggg catcagaacc cgttcaaagc
     2581 tcgccatcag cttttcgcgc tgcccgggcg gtaaatcctt ttcggcaaag gacaggacgc
     2641 cggatgtgta cttttttgcg tagggtgagg cgtcgataag ctccctgact tcttccggct
     2701 tcccctgcag aacggtggcg ccctcgcgct gtcggtcttt ccccagcaga taatccaccg
     2761 gaccggcgcc accgccgcgc cctcgcgggt gaaacttaac gatcatcgtc tgcacccttt
     2821 tccagcacgg catgccgcag ccgctcgagt ccggcatcta tggccatcag cgcggcgacg
     2881 acctgcacgc ggtcgtgccc gctgccacca ccggcgttaa cctgccgggc aatctggtta
     2941 aggttgttcc ccatgccggc aagctgacga agcagcgccg gcgagatcga gggaagcttg
     3001 ccggcgcggg cgggtttctc gtccaggcac gtctggcgca tccaggctgc aagctgttta
     3061 ccgtcgcagc gctccagcag acgccggtgc tcatcctcgg tcacccacat cgtgagcatt
     3121 ttgctgcgtt tgtctgccaa tccggcctcc tgataacgcc gggacgggcg ggaaatcttt
     3181 tcccggtcgg cgcggtgttg gacaagccgc aggcgcgtca gtggttttag cgggtttcgg
     3241 ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggctt aatatggtaa
     3301 atcgaagtgg gaattcaggg aagtgcttca tgtggcaggt aaaaaatgcg tctgtggggc
     3361 gtaagtgcga aatgctgggg acgatgaaat gacgcctcct cgctcactcg gtcgctacgc
     3421 tcctgccgtg agactgcggc gggcgttacc ggctcacgaa tggcgggata aagagatggc
     3481 gctcaaatca ggaagggctg ggaaaaggat gcggcgttgc cgtttttcca taggctccgc
     3541 ccccctgaca agcatcacga aatatgacgc tcaaatcagt ggcggcgaaa cccgacagga
     3601 ctataaagat cccaggcgtt tccccctggt agctccctcg tgcgctctcc tgttcctgcc
     3661 tttcggttta ccggtgtcat tccgctgtta tgtggccgcg tttgtctcat tccacgcctg
     3721 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga cccccccgtt
     3781 cagtccgact accacgccca ttccggtaac tatcaacttg agtccaaccc ggaaagacac
     3841 gacaaaatcg ccattggcgg tagccactgg taagcaggat gtgcgagaga tgtatcgaga
     3901 gttcttgaag tggtggccgg actacggcta cactggaagg acagatttgg tgacacgtct
     3961 cgcacaagac tgttaccacg gttcagaagt tccccaactt actgaacctt cgaaaaacca
     4021 cctccccagg tggttttttc gttttcagag caagagatta cgcgcagagc aaaacgatct
     4081 caagaagatc attttacaaa ttggataata tccctccgcc cctaacacta atataaaatt
     4141 atcgggcttg catgcccctc accattttcc ccatatccac caccaaacaa tagttcgtcg
     4201 agttctttgc tagcttgatc aatacacgca gccacatgaa tagcagaagg gtactcatca
     4261 tactgaaaat tatcgctttt ttcaggaggg agtcgatcat aatacggatg acaatccaca
     4321 taataatgac gtattagcat cccagtttta tcttcgtact ctgatggctg caacgtactc
     4381 cctgggaaag cttttgcaac atgtatagca gcttgaaagg tatcaaaata tcgcccaaac
     4441 attggcgttt caaagctagg cgactctgtc gaaaaatccc ccataacttg cgccaaataa
     4501 ccattatcta tctgccattg aacatctgat cccatagccc actctcccag agttactcct
     4561 aataaataac cataatatgt atttaccata acgcttcttg gccgcacccc ctgagagccc
     4621 ctcagcgcgt ttctgcggcc tgagggccgt ttccggcacc tttccccggc ccaaaccctg
     4681 aaaaacgccc ctgataattt ctgcgcggct gcatggctat gcacaagagt gaatggcggc
     4741 cggtttcgtg aaggaaatac
//
