LOCUS       NZ_AP025866             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80146 plasmid pFJMB80146_3,
            complete sequence.
ACCESSION   NZ_AP025866
VERSION     NZ_AP025866.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490875
            Assembly: GCF_023735615.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 198x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735615.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:09:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,297
            CDSs (total)                      :: 5,182
            Genes (coding)                    :: 5,085
            CDSs (with protein)               :: 5,085
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 28 of 97
            Pseudo Genes (incomplete)         :: 73 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            Pseudo Genes (short protein)      :: 1 of 97
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025866.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80146"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80146_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-06-24"
     gene            complement(join(2379..2511,1..95))
                     /locus_tag="NGJ48_RS26435"
     CDS             complement(join(2379..2511,1..95))
                     /locus_tag="NGJ48_RS26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(523..666)
                     /locus_tag="NGJ48_RS26440"
                     /old_locus_tag="FJMB80146_52090"
     CDS             complement(523..666)
                     /locus_tag="NGJ48_RS26440"
                     /old_locus_tag="FJMB80146_52090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(1827..2012)
                     /locus_tag="NGJ48_RS26445"
                     /old_locus_tag="FJMB80146_52110"
     CDS             complement(1827..2012)
                     /locus_tag="NGJ48_RS26445"
                     /old_locus_tag="FJMB80146_52110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1701..1780
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(502..1062)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 tcattggtgt gtatggcata gcttgtgcgc gtgcagccgg tgagggctga aagcgcaact
       61 gccgctgcga acacggaaac aaaaatcttt ttcaaagcca gctcctttta tccatatcag
      121 ttttggtcac taaccatcta actataggtc attttttatg tgaaaagagg cttgatggtg
      181 ggctgcatat cgagttaggt gcgaggtgtt atggttaaag tgtggtgtct ggcggggagg
      241 gggcagtgcg ggcttatatc tgatgtaatc cgaccgatac atataccaag atgatttaag
      301 tattcctctt tgtgaggttg gatgtctaat ttataaaagg atcttcttga gatccttttt
      361 tttcgcgcgt aatctcttgc cctgtaaacg aaaaaaccac ccggcaaggt ggtttttcga
      421 aggttcagca aaccggcaag tttctgaacc gtggtaacag ggtgtacaag accgctgcca
      481 ccaaatttgt ccttctagtg tagccgcagt caggcgtcaa cttcaagaac tctgcaccag
      541 taatcttgta taccccttac cagtggccgc tgccagtggc gtttttacgt gtctttccgg
      601 gttggactca agacgatagt taccggataa ggcgcagcag tcggactgaa cggggggttc
      661 gtgcatacag tccatcctgg agcgaactgc cttcccggaa ccgagtgtca ggcgtggaat
      721 gagaaaccgc ggccataaca gcggagtgac accggtaaac cgaaaggcag gaatgcgggg
      781 gagcacgagg gagccatcag ggggaaacgc ctggtatctt taagccgcat cgggtttcgc
      841 caccactgat ttgagcgtca gatttcgtga tgctcgtcag gggggcggag cctatggaaa
      901 aacggcttcg gcgcggcctt atgccttctt cgctaagtat cttcctggca tcccctagct
      961 ggcatcccct aggaaatttc tgctccaccc gtaagcccgt accgctcgcc gcagccgaac
     1021 gaccgagcgc agcgagtgag tgagcgagga agcggaatat atcctgtatt acattttctc
     1081 ctgacgcgtt ttctttcatt ttctgcgcct gtcttatgta gcagttatgc tatgcgttac
     1141 tgcggtgtta cattttagcc agtatacact ccgctagcgc tgagtgactg gtccggcgct
     1201 gcgcccggaa cccgcctgta ccggttcagc agccgttccg ggctgactgc aattttttta
     1261 ttttcctgcc cgctaccctg taaacctttc ttctgcgttg ccgttaacag gaggtaagcc
     1321 atgtttggtt tttcttccgg agacctgatg actcttgatg atgagcgaca cctgtttgtg
     1381 tctgttgttt cgggctggct ccggcgtgcc ggggggatgc cggagccatg atgctggatg
     1441 cataccggca gattttgtct gaaactgaac cggccgtaag gacggtcatg ctcgaatttc
     1501 ttgaatccgt tcgcattcat tacatttcgt cctgagcatt ttctgtattt ctgtccatac
     1561 ttaaacgagg cggccgtcac ggacgatggt cgtcgttgca tgatgctgtt atggatgagt
     1621 gaaagtaatc taatctaact tgacttacct tccgggcagc tccccggttt ttttttgcct
     1681 gttcccgggg aaccactcag gacaggatgt gtatttgtaa taccgcgcac gcgccggcat
     1741 cacaaatacc caggtcaggg ctgcgccccg acaccccgcc atcgcctcac ggccgcgcac
     1801 tgaattgcgc acccgttcac cgattttcac gcatcttcct cgcctgtctg tgcgttcagc
     1861 atggtgtaaa gtgcttcggc ctgatcgtgc aggtgctcac agcagccggc agcctcatcc
     1921 agatccatct gttcgagccg ctcgaggagg aggagtgtct gtgagcggat aaatttggcc
     1981 atgttgaggg cggcggattc ctgtctggtc actggtgatt ttccccgtct ggtgatgatt
     2041 tttgcgtgaa aagagcatgc cgtcattctg gtgcatagtc atgcatgccg ttaaaattta
     2101 acaggagcgt ttctggcggg ttccggggtg gtttgttgtg gtttttggtc atggttccgt
     2161 cagaaaagcg ctgagtgcgt ctgaggcggt gcgggcaacg gatgttatgg taaattcttg
     2221 gggggcttga ttataaaaac cgcacttcct ctaatgtctt gtgcttttta gacaaccctg
     2281 gaagctttta gctttggtgg tgcaatttgc tgggattttg gcggagaacc ccaggtaaag
     2341 aaaaaggcca cattagcggc ctttttcgga gaagatgctc agtgcggaat ttcggagact
     2401 tctttaacgt cggttttatt gatctgctgt ttgacaccat tcgcgtctgt atagccaagc
     2461 aggcctgagt ctgattcttt aggtttgccg tcgctgacaa tagtgcgccc g
//
