LOCUS       NZ_AP025865             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80146 plasmid pFJMB80146_2,
            complete sequence.
ACCESSION   NZ_AP025865
VERSION     NZ_AP025865.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490875
            Assembly: GCF_023735615.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 198x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735615.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:09:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,297
            CDSs (total)                      :: 5,182
            Genes (coding)                    :: 5,085
            CDSs (with protein)               :: 5,085
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 28 of 97
            Pseudo Genes (incomplete)         :: 73 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            Pseudo Genes (short protein)      :: 1 of 97
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025865.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80146"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80146_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-06-24"
     gene            217..432
                     /locus_tag="NGJ48_RS26405"
                     /old_locus_tag="FJMB80146_52060"
     CDS             217..432
                     /locus_tag="NGJ48_RS26405"
                     /old_locus_tag="FJMB80146_52060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            703..1617
                     /gene="lpxO"
                     /locus_tag="NGJ48_RS26410"
     CDS             703..1617
                     /gene="lpxO"
                     /locus_tag="NGJ48_RS26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            2017..2478
                     /locus_tag="NGJ48_RS26415"
     CDS             2017..2478
                     /locus_tag="NGJ48_RS26415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(2733..2843)
                     /locus_tag="NGJ48_RS26420"
     CDS             complement(2733..2843)
                     /locus_tag="NGJ48_RS26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            3487..3810
                     /locus_tag="NGJ48_RS26425"
                     /old_locus_tag="FJMB80146_52080"
     CDS             3487..3810
                     /locus_tag="NGJ48_RS26425"
                     /old_locus_tag="FJMB80146_52080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4019..>4453
                     /locus_tag="NGJ48_RS26430"
                     /pseudo=""
     CDS             4019..>4453
                     /locus_tag="NGJ48_RS26430"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3200..3383
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(2734..3217)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          4..530
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.523"
                     /coverage="99.0"
                     /evalue="2.79e-81"
     Typing          3801..4759
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.562"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aacgcggcca ttctgtggtg gcagagtcag cagatactcg acaattacgt gagcatccgg
       61 gagcagaaga gcacgcaggc gatgctgtcc gagcggaaca gcggcgtgca gctctcgacc
      121 tgcggcgagc agagacgccg ctgcgtgagg gtgaacccgg aagcgggacg gttcggagag
      181 gattcgagct ggatgatact ggcggggaaa tagcacatga cagagctgga aaaacagttg
      241 ctgagcgcat tagagcagct acagcaggac tactcgaaaa ggctggacga gtgggagagc
      301 gccttcgcgg aatggcggac gatgtctggt cttatgcaac gggagaacgc ggcgctgagc
      361 gagcgcgtca cgggcttgag caggcaggtg cagagtttga gcgatcagct gcgccggttg
      421 tcgagcggct gaatgccgtc gagctgcagc aggtgcggga ggcgcggcat cagaaagcgc
      481 tggagctcga acgatcacag cgacagcggg tatatcacgg tccgtccctt tgattcattt
      541 cagcataatt cctgctgaaa tcctctctgc ctgtgtagta gatatgttct ggtgaagctt
      601 cgattatact attgataaat agcatttaga ttataattta agtgcattca ttatgttatc
      661 tttaagagat ttttattatt ggcgtgaaca aggaatatac acatgctgtc acttattatt
      721 atttttgttt ttcttatcag tgtattgtat gcgcattctc gcggtaaaat aaaacaaaag
      781 ttttccaggc agttatttga tcattcaaca tttatggctc cgataaatat gtttatgaca
      841 ggcttttcga agctgccgtc aaagcaggca tggtttcccc cagaaacatt tcctgaactt
      901 caaccattaa ttgataactg gcaggttata agagacgaag ctgttaattt gcaagggcac
      961 attaaatcat ctgaaaaaaa taatgatgca ggatttaata cattttttaa gcgcggttgg
     1021 aagcgctttt acctcaagtg gtatggagat gctcatccat cagcggagcg gctttgccca
     1081 gtaacaaccc aactggttag ctctattccc tgtattaaag cagcgatgtt tgctgaattg
     1141 ccttcaggaa gccatttagg aaagcatcgt gacccttatg ccggttcagt tcgttatcat
     1201 ttaggattat caacaccaaa tgatgaacgg tgttttattg aagttgatgg tgtacgccgc
     1261 agttggcatg atggtgaagt gataattttt gacgaaactt ttgttcactg ggctcagaat
     1321 gataccgagc agacacgtat tattttgttc tgtgatatag aaaggccgat gaagtggcgg
     1381 tggtcacaaa aaataaatag gtggataggt ggggtgctaa tgtcagcggc agcatcaccc
     1441 aacgataata atgacaggac tggtgctata aatcatttat ttaaatatgt taatgctatt
     1501 cgtgatgcag gtcagaagct aaaaaaaagg aatcgaaaac tatattattt gattaaaaat
     1561 ggagttattt ctttgatttt tgtttggctt gctgtctcag tgtttagatg gctgtgattt
     1621 gtttttttct attgagtcat acataaattt gggtcttcta tgctcaatgt atggttatag
     1681 ataaaaatcg cctgctaaaa aattctattg aaatcttttt agtaagaaag gcccgaatct
     1741 gacgctcagc gggcctttcg tgaagaagtg cggcagtaga cagatttcat ctgatcacgt
     1801 tcatgtgctg ctgtgcaatt aagcgtttct ttcggagctt tcacggtatt tccttcacga
     1861 aaccggccgc cattcactct tgtgcatagc catgcagccg cgcagaaatt atcaggggcg
     1921 tttttcaggg tttgggccgg ggaaaggtgc cggaaacggc cctcaggccg cagaaacgcg
     1981 ctgaggggct ctcagggggt gcggccaaga agcgttatgg taaatacata ttatggttat
     2041 ttattaggag taactctggg agagtgggct atgggatcag atgttcaatg gcagatagat
     2101 aatggttatt tggcgcaagt tatgggggat ttttcgacag agtcgcctag ctttgaaacg
     2161 ccaatgtttg ggcgatattt tgataccttt caagctgcta tacatgttgc aaaagctttc
     2221 ccagggagta cgttgcagcc atcagagtac gaagataaaa ctgggatgct aatacgtcat
     2281 tattatgtgg attgtcatcc gtattatgat cgactccctc ctgaaaaaag cgataatttt
     2341 cagtatgatg agtacccttc tgctattcat gtggctgcgt gtattgatca agctagcaaa
     2401 gaactcgacg aactattgtt tggtggtgga tatggggaaa atggtgaggg gcatgcaagc
     2461 ccgataattt tatattagtg ttaggggcgg agggatatta tccaatttgt aaaatgatct
     2521 tcttgagatc gttttgctct gcgcgtaatc tcttgctctg aaaacgaaaa aaccacctgg
     2581 ggaggtggtt tttcgaaggt tcagtaagtt ggggaacttc tgaaccgtgg taacagtctt
     2641 gtgcgagacg tgtcaccaaa tctgtccttc cagtgtagcc gtagtccggc caccacttca
     2701 agaactctcg atacatctct cgcacatcct gcttaccagt ggctaccgcc aatggcgatt
     2761 ttgtcgtgtc tttccgggtt ggactcaagt tgatagttac cggaatgggc gtggtagtcg
     2821 gactgaacgg gggggtcgtg catacagtcc agcttggagc gaactgccta cccggaactg
     2881 agtgtcaggc gtggaatgag acaaacgcgg ccacataaca gcggaatgac accggtaaac
     2941 cgaaaggcag gaacaggaga gcgcacgagg gagctaccag ggggaaacgc ctgggatctt
     3001 tatagtcctg tcgggtttcg ccgccactga tttgagcgtc atatttcgtg atgcttgtca
     3061 ggggggcgga gcctatggaa aaacggcaac gccgcatcct tttcccagcc cttcctgatt
     3121 tgagcgccat ctctttatcc cgccattcgt gagccggtaa cgcccgccgc agtctcacgg
     3181 caggagcgta gcgaccgagt gagcgaggag gcgtcatttc atcgtcccca gcatttcgca
     3241 cttacgcccc acagacgcat tttttacctg ccacatgaag cacttccctg aattcccact
     3301 tcgatttacc atattaagcc agtatacact ccgctagcgc tacgtgactg gttcagggct
     3361 gcgccccgaa acccgctaaa accactgacg cgcctgcggc ttgtccaaca ccgcgccgac
     3421 cgggaaaaga tttcccgccc gtcccggcgt tatcaggagg ccggattggc agacaaacgc
     3481 agcaaaatgc tcacgatgtg ggtgaccgag gatgagcacc ggcgtctgct ggagcgctgc
     3541 gacggtaaac agcttgcagc ctggatgcgc cagacgtgcc tggacgagaa acccgcccgc
     3601 gccggcaagc ttccctcgat ctcgccggcg ctgcttcgtc agcttgccgg catggggaac
     3661 aaccttaacc agattgcccg gcaggttaac gccggtggtg gcagcgggca cgaccgcgtg
     3721 caggtcgtcg ccgcgctgat ggccatagat gccggactcg agcggctgcg gcatgccgtg
     3781 ctggaaaagg gtgcagacga tgatcgttaa gtttcacccg cgagggcgcg gcggtggcgc
     3841 cggtccggtg gattatctgc tggggaaaga ccgacagcgc gagggcgcca ccgttctgca
     3901 ggggaagccg gaagaagtca gggagcttat cgacgcctca ccctacgcaa aaaagtacac
     3961 atccggcgtc ctgtcctttg ccgaaaagga tttaccgccc gggcagcgcg aaaagctgat
     4021 ggcgagcttt gaacgggttc tgatgcccgg actcgacaaa gaccagtaca gcgtgctgtg
     4081 ggttgagcac caggataagg ggcggctgga gctgaacttc ctgatcccga acacggagct
     4141 gctgactggc aagcgtctcc agccgtatta cgaccgcgcc gaccgtccgc gcattgatgc
     4201 ctggcagacc atcgtgaacg gcaggctggg gctgcatgac ccgaacgcgc ctgaaaaccg
     4261 gcgggcgctg gtcacgccgt ccgcgctgcc ggaaacgaag caggaagccg ctcaggcaat
     4321 tacacggggt ttactggcct tggcctcgtc cggggagctg aaaacccgcc aggacgtcac
     4381 tgaggcgctg gaaagcgcag gttttgaggt ggtacgcacc acaaaaagca gcatcagcat
     4441 tgccgacccg gacggggggc gaaacatccg actgaaggga gccatctatg aacagtcttt
     4501 taacgctggc gaaggactta gagcagaaat cgaaagcgca gcagcagagt accggcgaga
     4561 tgctgaaagc cgcattcagc gagcacgaga agtctgtcag agcggaactg agcgaaagcg
     4621 agaagagaat cagcgccgcc atcctcgacc acgaccggaa gctgtcctca gccatgagcc
     4681 agcgtacgaa agggatgctg cgcatggtca gccagacgtg gctgaccatc gtcctggtct
     4741 ccgtgctgct gatcgcctcg
//
